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Citations for Promega :
5651 - 5700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
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bioRxiv - Immunology 2023Quote: ... Promega GoTaq qRT-PCR kit (Promega, Fitchburg USA) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl of Bright-Glo™ Luciferase Assay lysis/substrate buffer (Promega # E2620) were added and luminescence was measured after 10 min using a Synergy H1 plate reader (Bio-Tek ...
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bioRxiv - Microbiology 2023Quote: ... 200 ml of media was treated with 4 U of RQ1 RNase-free DNase (Promega) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... goat α-mouse IgG antibodies (Promega) and donkey α-goat IgG (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... lactate dehydrogenase (LDH) was measured from leukocyte supernatants using CytoTox 96 Non-Radioactive Cytotoxicity kit (Promega; Cat. No. g1780) per manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... single cell clones were first screened for insertion of an in-frame repair template using the Nano-Glo HiBit Lytic Detection System (Promega). HiBit-positive clones were then expanded and screened by anti-CD19 Western blot and genomic DNA PCR for genotyping.
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bioRxiv - Microbiology 2023Quote: ... Plates were washed in PBS/0.05% Tween 20 (Promega) and then blocked with blocking buffer solution (PBS/0.05% Tween 20 /3% non-fat milk (AmericanBio ...
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bioRxiv - Microbiology 2023Quote: ... Fugene (HD) (Promega, #E2311); Opti-MEM (Gibco™ ...
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bioRxiv - Microbiology 2023Quote: ... Biotin-oligo d(T) (Promega, #Z5261, 0.2 μmol/L for mRNA FISH); Diethyl Pyrocarbonate (DEPC)-treated water (Invitrogen™ ...
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bioRxiv - Microbiology 2023Quote: ... M-MLV (Promega, #M1701); Random primers (Invitrogen™ ...
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bioRxiv - Microbiology 2023Quote: DNA was extracted using the Maxwell® RSC Viral Total Nucleic Acid Purification kit (Promega), as per the manufacturer’s instructions into nuclease-free water ...
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bioRxiv - Immunology 2023Quote: ... Each reaction was run in duplicate and prepared with GoTaq master mix (Promega Corporation, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega, M0482S) with qTOWER3 (Analytik Jena) ...
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bioRxiv - Immunology 2023Quote: ... total RNA was purified from 3-4 tissues of female flies using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). Quantitative RT-PCR was performed using a OneTaq RT‒PCR kit (Promega ...
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bioRxiv - Immunology 2023Quote: ... Total RNA was purified using a ReliaPrep RNA Tissue Miniprep kit (Promega, z6112). The RNA was sent to Kazusa Genome Technologies to perform the library preparation and sequencing ...
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bioRxiv - Immunology 2023Quote: ... which was isolated from BMDCs using the ReliaPrep RNA Cell Miniprep System (#Z76012, Promega, Madison, USA), using ReverTra Ace qPCR Master Mix (#FSQ-201 ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed once with PBS and lysed for at least 1 h with 40 μl 1x passive lysis buffer (Promega) at RT ...
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bioRxiv - Immunology 2023Quote: ... and luciferase activity was measured in a Tecan infinite 200Pro by automatic injection of 50 μl luciferase assay substrate (Promega) and 10 sec integration time.
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bioRxiv - Molecular Biology 2023Quote: ... NanoBRET NanoGlo Substrate (Promega) solution was added to each well ...
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bioRxiv - Molecular Biology 2023Quote: ... penicillin (100 U/ml) and streptomycin (100 μg/ml) in the presence or absence of compounds and HaloTag NanoBRET 618 Ligand (Promega) for 4 h ...
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bioRxiv - Molecular Biology 2023Quote: ... The released peptides were then transferred over to a new tube and a 4 h digest using 0.5 ug mass spectrometry-grade trypsin (V5111, Promega) at 37 C for 4 hours was conducted ...
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bioRxiv - Molecular Biology 2023Quote: ... they were multiplexed with the CellTiter-Glo 2.0 Cell Viability Assay (G9241, Promega) following the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection was carried out using the FuGENE HD Transfection Reagent (E2312, Promega) according to the manufacturer’s guidelines in the Opti-MEM I Reduced-Serum Medium (31985047 ...
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bioRxiv - Molecular Biology 2023Quote: ... the cell viability was assessed using the CellTiter Blue kit (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 500 ng of Trypsin (V5111, Promega) for an additional 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... HiBiT fusion vectors were constructed using the parental vector pBiT3.1-N (N2361, Promega). To generate the HiBiT-VHL vector ...
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bioRxiv - Molecular Biology 2023Quote: The bioluminescence resonance energy transfer (BRET) ubiquitination assay was performed using the NanoBRET Nano-Glo Detection Systems (Standard) (N1661, Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... and pNLF1-C (N1361, Promega) to obtain N-terminal and C- terminal NanoLuc-tagged protein fusions ...
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bioRxiv - Molecular Biology 2023Quote: ... Transfection was carried out using the FuGENE HD Transfection Reagent (E2312, Promega) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... NanoLuc fusion vectors were constructed using the parental vectors pNLF1-N (N1351, Promega) and pNLF1-C (N1361 ...
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bioRxiv - Molecular Biology 2023Quote: ... The cold mixture was quickly transferred to a PCR-clean up column (Promega, A9282) and centrifuged for 30 s at 14,000g ...
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bioRxiv - Molecular Biology 2023Quote: ... using 2 µl of Fugene HD transfection reagent (Promega). The control reporter plasmid was transfected in parallel in equimolar amounts with pEGFP or pPRKRA ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR amplifications were performed using GoTaq DNA Polymerase (Promega, M3001), according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... RNasin Ribonuclease Inhibitor (Promega, N2515) in nuclease-free water ...
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bioRxiv - Molecular Biology 2023Quote: ... RNasin Ribonuclease Inhibitor (Promega, N2515) in nuclease-free water.
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bioRxiv - Molecular Biology 2023Quote: ... Luminescence was measured for each N1-X/N2-Y pairwise combination by auto-injecting 50 µL Renilla luciferase substrate (Renilla Luciferase Assay system, catalog No. E2820, Promega) per well and integrating light output for 4 s using a TriStar luminometer (Berthold) ...
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bioRxiv - Molecular Biology 2023Quote: ... 50 µL/well of the Renilla luciferase substrate (Renilla Luciferase Assay system, cat#E2820, Promega) were then added and luminescence was read approximately two minutes after injection using a Centro XS3 LB960 plate reader (3 s integration) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured using a BrightGlo kit from Promega (Madison, WI).
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bioRxiv - Molecular Biology 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) was added as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... the Nano-Glo HiBiT Lytic Detection Assay (N3040, Promega) was performed according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2023Quote: ... then 500 ng of Trypsin/LysC (V5071, Promega, Madison, WI, USA) for 16 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length GID4 N or C-terminal NanoLuc-fusion was transfected into HEK293T cells using FuGENE HD (Promega, E2312) according to manufacturer’s instructions and proteins were allowed to express for 20 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were washed three times with PBSTR (PBS containing 0.1% Tween-20 (Promega, 0000395426), 0.1 U/µL RRI (Vazyme ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase assays were conducted using the Dual-Luciferase Reporter Assay System (Promega). HeLa cells were seeded at 70,000 cells per well of a 24-well plate approximately 24 hours prior to transfection ...
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bioRxiv - Molecular Biology 2023Quote: ... and another 10 μl the NanoLuc substrate (furimazine, 1:25 dilution, Promega) diluted in the detection buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... the proteins were digested in-gel using trypsin (modified, sequencing purity, Promega), as previously described71 ...
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bioRxiv - Molecular Biology 2023Quote: ... or empty vector control (400ng) with 2.4 ul Fugene HD (Promega, E2311) in 43 ul Opti-MEM media (Gibco) ...
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bioRxiv - Molecular Biology 2023Quote: The human MEOX1 enhancer syntenic to the mouse Meox1 peak9/10 enhancer3 fragment along with the mouse Il1b peak5 and peak6 fragments were PCR amplified and cloned into the XhoI and HindIII sites in the Firefly luciferase plasmid pGL4.23 (Promega) using the Cold Fusion kit (System Biosciences Inc ...