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Citations for Promega :
5701 - 5750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The pGL3 vector-based constructs (Promega, E1751) described earlier (17 ...
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bioRxiv - Molecular Biology 2023Quote: ... and the SV40pro+SV40e (Promega, E1741) vectors were used to evaluate promoter and enhancer activity ...
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bioRxiv - Molecular Biology 2023Quote: ... gene activity was assessed according to the supplied Luciferase Assay System Kit protocol (Promega). Luminescence detection was performed using the Microlumat LB96p device (EGG Berthold ...
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bioRxiv - Molecular Biology 2023Quote: ... the SV40pro (Promega, E1761) and the SV40pro+SV40e (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... Gel slices were pre-incubated with 20 μl of pre-activated trypsin (Promega France, Charbonnières) at room temperature for 15-20 min ...
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bioRxiv - Molecular Biology 2023Quote: The synthesis of double-stranded RNA (dsRNA) was followed by the manufacturer’s instructions of the T7 RiboMAXTM Express RNAi System (Promega, USA). Briefly ...
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bioRxiv - Molecular Biology 2023Quote: ... digested with SacI (Promega) and separated by electrophoresis in 0.8% agarose (Methods S1) ...
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bioRxiv - Molecular Biology 2023Quote: ... provided by the Nano-Glo® HiBiT Blotting System (PROMEGA N2410). Readout was carried out in a ChemiDoc XRS+ (BioRad).
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bioRxiv - Molecular Biology 2023Quote: ... prepared following the FuGENE HD (PROMEGA) protocol was added to 50 μl of seeded cells ...
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bioRxiv - Molecular Biology 2023Quote: PROTAC toxicity was determined by using the CellTiter-Glo® 2.0 Cell Viability Assay (PROMEGA G9241). For this ...
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bioRxiv - Molecular Biology 2023Quote: Full-length VHL was obtained as plasmids cloned in frame with a terminal NanoLuc-fusion (Promega, ND2700). The plasmid was transfected into HEK293T cells using FuGENE HD (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Nano-Glo® HiBiT Lytic Detection System (PROMEGA N3030) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Serially diluted inhibitor and NanoBRET CRBN Tracer (Promega) at a concentration previously determined (1 μM ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 ml FuGENE HD (Promega, E2312) transfection mix containing 30 μl FuGENE HD and 8 μl of each plasmid was prepared per plate ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation at 37 °C/5 % CO2 for 20 h 40 nl HaloTag® NanoBRET™ 618 Ligand (PROMEGA) was added to the cells using an Echo acoustic dispenser (Labcyte ...
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bioRxiv - Molecular Biology 2023Quote: ... NanoBRET NanoGlo Substrate + Extracellular NanoLuc Inhibitor (Promega, N2540) were added as per the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... using the Nano-Glo® HiBiT Blotting System (PROMEGA N2410). Here ...
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bioRxiv - Molecular Biology 2023Quote: ... The plasmid was transfected into HEK293T cells using FuGENE HD (Promega, E2312) and proteins were allowed to express for 20h ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Molecular Biology 2023Quote: ... The 3,199-bp pGEM3 Zf(+) was used as a source of circular ssDNA and dsDNA (Promega Biotech, Spain). DNA concentrations were established using the molar extinction coefficients of 8780 and 6500 M-1 cm-1 at 260 nm for ssDNA and dsDNA ...
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bioRxiv - Molecular Biology 2023Quote: ... together with dNTP mix containing 10 mM of each nucleotide (Promega). Combined reaction mixtures were incubated at 50°C for 10 minutes prior to inactivation at 85°C for 10 minutes.
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bioRxiv - Molecular Biology 2023Quote: ... in the presence of 1 mM ATP and 1 unit/uL RNasin® (Promega, N2515) for 30 minutes at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Linear plasmids were transcribed in vitro using the T7 RiboMAX Express Large Scale RNA Production System (Promega) using ‘half sized’ reactions whereby 250 ng linear plasmid was added to a reaction mixture using half the volume suggested for all reagents in the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL 10 mM dNTPs (Promega, #C1141), 10 µL 5X LongAmp Taq Reaction Buffer in 50 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL 10 mM dNTPs (Promega, #C1141), 10 µL 5X LongAmp Taq Reaction Buffer in 50 µL ...
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bioRxiv - Molecular Biology 2023Quote: ... 1.5 µL 10 mM dNTPs (Promega, #C1141), 10 µL 5X LongAmp Taq Reaction Buffer in 50 µL ...
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bioRxiv - Molecular Biology 2023Quote: Protein samples were treated with Endoprotease Lys-C (Fujifilm Wako chemicals, Osaka, Japan) and Trypsin (Trypsin Gold Mass Spec Grade, Promega). Peptide samples were desalted using OMIX C18 pipette tips (Agilent Technologies) ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activity was measured with the Luciferase Reporter Assay Kit (Promega).
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bioRxiv - Molecular Biology 2023Quote: ... HAP1 and HAP1-dEGFPY66S cells were imaged in imaging medium (FluoroBrite™ DMEM, 10% FBS, 2.5 mM CaCl2) supplemented with 2 µL CellTox™ Green (Promega, G8741) and 5 µL Annexin V-AV647 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... qPCR was performed using GoTaq qPCR Master Mix (Promega). Reaction mixes were made up in 10 μL total volume containing 500 nM forward and reverse primers (Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... Luciferase activities were measured by Dual-Glo luciferase assays (Promega) and normalized to BSA.
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bioRxiv - Molecular Biology 2023Quote: In vitro translation was performed using a rabbit reticulocyte lysate system (Promega, L4960). 1.5 μL of purified eIF2A protein or BSA was added to 10 μL rabbit reticulocyte lysate reaction mix ...
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bioRxiv - Molecular Biology 2023Quote: ... 1 µL of 100 ng/µL random hexamer primers (Promega: C1181) was added to each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... or the control vector pHTN HaloTag CMV-neo (G7721, Promega) and 0.01 or 0.02 µg NanoLuc-tagged SOCS1 or VHL expression constructs ...
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bioRxiv - Microbiology 2023Quote: The genes were cloned one by one into in pGEM-T Easy Vector (Promega, Madison, USA) and finally in pMG36e vector (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... The amplified products were stained with a mix of 2X Blue/Orange Loading Dye (Promega, Madison, USA), loading buffer (1X ...
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bioRxiv - Microbiology 2023Quote: ... lactis strains were extracted using a “Wizard Genomic DNA Purification” kit (Promega, Madison, USA). Real-time PCR was done using a Rotor-Gene Q machine (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... The pGEM-T Easy Vector (Promega, Madison, USA) and pMG36e vector (Addgene ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Molecular Biology 2023Quote: ... 1.5μg of plasmid was transfected into 6-well dishes using Fugene HD (Promega, Madison, WI, USA) with 3:1 transfection reagent to DNA for 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... 8μl of Trypsin/LysC solution (100ng/μl 1:1 Trypsin (Promega) and LysC (FujiFilm ...
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bioRxiv - Microbiology 2023Quote: ... 100 μl CellTiter-Glo Reagent (CellTiter-Glo Luminescent Cell Viability Assay, Promega, cat. G7571) was added to each well for 10 min before luminescence was measured with PerkinElmer EnSight microplate reader ...
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bioRxiv - Microbiology 2023Quote: DNA of selected phage-resistant mutants was isolated using a Wizard Genomic DNA purification kit (Promega Corp.) according to the guidelines provided by the manufacturer ...
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bioRxiv - Microbiology 2023Quote: ... Cell supernatants were assayed for glutamine consumption (Promega #J8021) and Lactate production (Promega #J5921 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was quantified using the Quantus system (Promega Corp.). The integrity of samples was analyzed with TapeStation 2200 (Agilent) ...
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bioRxiv - Microbiology 2023Quote: ... The samples were then cleaned up using the ProNex Size-Selective Purification System (Promega, Wisconsin, USA) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and Lactate production (Promega #J5921) according to the kit specifications ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were digested with MS Grade trypsin (Promega, Madison, WI, USA) on 10 kDa Microcons (Merck-Milipore ...
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bioRxiv - Microbiology 2023Quote: ... A 1 kb DNA ladder (Promega) was used as a size marker ...
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bioRxiv - Microbiology 2023Quote: ... All diagnostic PCR analyses were performed using GoTaq Green (Promega) under the following conditions ...