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Citations for Promega :
5501 - 5550 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Concentrated viruses were titrated in serial dilutions with the respective target cell lines and the luciferase reporter signal was determined 48 h later using Luciferase Assay System (Promega, catalog no. E1501) on Cytation 3 (BioTek) ...
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bioRxiv - Pathology 2019Quote: ... Cells were then infected with adenovirus as aforementioned and cultured for additional 24 hr before lysis in Bright-Glo (Promega; catalog no. 2610). Luminescence signals were measured on a FlexStation 3 Benchtop Multi-Mode Microplate Reader (Molecular Devices ...
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bioRxiv - Plant Biology 2019Quote: ... 2 μL of each ligation reaction were used for heat-shock transformation of 50 μL of JM109 High-Efficiency Competent Cells (Promega, Madison, WI, USA), following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were authenticated at the Duke University DNA Analysis Facility by analyzing DNA samples from each cell lines for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA). HCT116 cell line was a gift from Dr ...
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bioRxiv - Cancer Biology 2019Quote: ... a proliferation rate was calculated using the data obtained from MTT colorimetric assays (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega; Madison, WI). Data from five replicates for at each time point were analyzed using Graphpad Prism software with a p value less than 0.05 considered significant.
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bioRxiv - Immunology 2020Quote: ... ExpiCHO cells then were incubated with Jurkat cells expressing FcγRIIIa receptor or FcγRIIa on their surface and stably transfected with NFAT-driven luciferase gene (Promega, Cat. Nr.: G9798 and G7018) at an effector to target ratio of 6:1 for FcγRIIIa and 5:1 for FcγRIIa ...
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bioRxiv - Pathology 2021Quote: ... Transient knockdowns were performed with mycoplasma free cells using 100 nM siRNAs comprising of three different probes (Gene Pharma, Shanghai, China) and FugeneHD (Promega, Madison, WI, USA) for 24 hours ...
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bioRxiv - Microbiology 2020Quote: ... Cells were lysed 48 hr post-transfection in co-IP buffer supplemented with both protease inhibitors and RNAsin (Promega N2515, 100 units/mL). Lysates were processed and immunoprecipitations were performed as described above for the polymerase formation assay ...
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bioRxiv - Microbiology 2021Quote: ... A 2 ml aliquot of the overnight enrichment was centrifuged at 6000 RPM for 10 minutes and then DNA was extracted using the Maxwell RSC Cultured Cells DNA Kit (Promega, Madison, WI, USA). Extraction was performed according to manufacturer’s recommendations with the addition of RNase A after lysis ...
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bioRxiv - Microbiology 2021Quote: ... Host cell apoptosis was measured in rabbit lung sections using the DeadEnd Colorimetric TUNEL System following the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Apoptotic and non-apoptotic cells were counted manually using the EVOS FL microscope (ThermoFisher Scientific ...
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bioRxiv - Genomics 2020Quote: ... Another 1 ml portion of each spiked enrichment sample was extracted using the Maxwell RSC Cultured Cells DNA kit with a Maxwell RSC Instrument (Promega Corporation, Madison, WI) according to manufacturer’s instructions for Gram-negative bacteria with additional RNase treatment ...
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bioRxiv - Cancer Biology 2020Quote: ... 1000 cells were seeded in 96-well plates with five replicates and the cell growth capacity was measured every day with the CellTiterBlue assay (#G8080, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were seeded on poly-lysine coated glass coverslips at a concentration of 1 × 105 cells/ml and transiently transfected using the FuGENE® HD Transfection Reagent (Promega, Madison, WI) two days before measurement.
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bioRxiv - Cancer Biology 2019Quote: ... 5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay (Celltiter 96 Aqueous One-solution Cell Proliferation Assay, Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We extracted genomic DNA from 400 µL of these cultures with the Maxwell® RSC Cultured Cells DNA Kits using a Maxwell® RSC Instrument (Promega, Switzerland), at the Functional Genomics Centre Zurich (FGCZ) ...
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bioRxiv - Physiology 2021Quote: ... islets or cells were washed with PBS and the bioluminescence reaction was initiated by addition of BacTiter-Glo™ reagent (Promega, Madison, WI) and maintained for 5 min at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... Single-cell clones were obtained and the genomic DNA was then isolated using a Wizard SV genomic DNA purification kit (Promega, Madison, WI, USA). The target gene was amplified by PCR using pMXs vector forward (5’GGTGGACCATCCTCTAGA3’ ...
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bioRxiv - Cancer Biology 2020Quote: ... service by analyzing DNA samples from each individual cell line for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA).
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bioRxiv - Cancer Biology 2022Quote: ... the cells were harvested in the lysis buffer and the fluorescence value was measured by a dual luciferase assay kit (Promega, Madison, WI, USA).
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bioRxiv - Microbiology 2022Quote: ... genomic DNA extraction was performed using a lysozyme/mutanolysin-based cell lysis followed by purification using the Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA). Library preparation on extracted DNA was conducted using a Kapa kit (Roche ...
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bioRxiv - Molecular Biology 2022Quote: These recombinant plasmids were co-transfected with the components of Dharmacon™ Trans-Lentiviral packaging kit into HEK293FT cells using FuGENE® HD (Promega Corp.) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: Cell growth and survival was measured using a CellTiter 96® Non-Radioactive Cell Proliferation Assay at an absorbance of 570 nm using (Promega, Mannheim, Germany). Each assay was performed in triplicates and repeated at least 3 times ...
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bioRxiv - Molecular Biology 2024Quote: ... A total of 350 µL of this aqueous phase underwent automated extraction and cleaning using a Tecan Freedom EVO liquid handler with Promega Purefood protocol and reagents (cell lysis buffer, ReliaPrep™ resin, prepared wash buffer, isopropanol, 50% ethanol and Promega elution buffer). The total extracted and washed DNA was eluted in a final volume of 150 µL.
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bioRxiv - Microbiology 2024Quote: ... the viability of HAEs was assessed by a MTS assay (CellTiter 96® AQueous One Solution Cell Proliferation Assay; Promega, Madison, WI, USA) by addition of 20 µl of MTS solution with 180 µl of Opti-MEM on the apical pole of HAEs ...
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bioRxiv - Microbiology 2024Quote: HeLa cells were transfected with plasmids encoding ByeA or ByeAC197A according to the FuGENE transfection protocol (FuGENE® HD Transfection Reagent, Promega Cat. E2311) as advised by the manufacturer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: The cytotoxicity of NBCoV small molecules in the different cell types was performed in parallel with the neutralization assay and evaluated using the colorimetric CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Madison, WI) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Luciferase expression in cells was measured by lysing the cells in Passive Lysis Buffer and incubating the lysis supernatant with the Luciferase Assay System (Promega, Madison, WI, USA). Luciferase activity was read on an Enspire Multimode Plate Reader (Perkin Elmer) ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were resuspended in PBS and dispensed in a white opaque 96-well microplate containing BacTiter-Glo reagent (Promega, Madison, WI, USA). The resulting 1:1 mixture was incubated for 5 min and luminescence was measured as a function of ATP content using a microplate reader.
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bioRxiv - Biochemistry 2023Quote: The cell free protein expression assays were performed by using the TnT® Quick Coupled Transcription/Translation System (Cat#L1171, Promega, Madison, USA), according to the manufacturer’s guideline with minor modification ...
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bioRxiv - Cancer Biology 2023Quote: ... viable cells were quantified in a FLUOstar OPTIMA ELISA reader (550/590 nm) about 2 h after CellTiter-Blue staining (Promega, Cat No. G8081). Mean values +/-standard deviations (SD ...
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bioRxiv - Cancer Biology 2022Quote: ... and West African ancestry HCC1500 (ATCC, CAT: CRL-2329) was assessed utilizing CellTiter-Glo 2.0™ Luminescent Cell Viability Assay (Promega™, CAT: G9242). West African ancestry of the HCC1500 cell line was determined using established Ancestry Informative Markers [38] ...
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bioRxiv - Immunology 2023Quote: T-cell and prodrug cytotoxicity assays with secreted enzymes were performed after 24 to 48 hours using CellTiter-Glo (Promega, Madison, Wisconsin, USA) or luminescence using firefly (ffLuc ...
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bioRxiv - Cancer Biology 2023Quote: ... Authentication of cell lines was confirmed regularly by short tandem repeat (STR) profiling by using the GenePrint 10 kit (Promega, Madison, WI, USA) on an ABI 3730 DNA analyzer (Applied Biosystems ...
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bioRxiv - Biochemistry 2023Quote: ... AGO1/2/3-/- cells were transfected with WT-AGO2-GFP or NES-AGO2-GFP plasmids with OptiMEM and FuGENE HD Transfection Reagent (Promega, Cat. No. E2311) with media change to full media at 24 hours post-transfection ...
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bioRxiv - Immunology 2023Quote: ... cells were further cultivated for 36 h and harvested for luciferase assay using the Nano-Glo luciferase assay system (Promega; cat. no. N1120) and the GloMax Navigator Microplate Luminometer (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... was amplified from complementary DNA synthesized by reverse transcriptase starting from RNA of HAP1 WT cells (GoScriptTM Reverse Transcriptase, Promega, Madison WI, USA). HMBS_232 was cloned into pCMV-puroR lentiviral plasmid using the primers listed in Table S6 and NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs) ...
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bioRxiv - Synthetic Biology 2024Quote: Genomic DNA was isolated from plant cells using the Promega Wizard® SV 96 Genomic DNA Purification System (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol using the Tecan Fluent (Supplementary Figure S2E).
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bioRxiv - Cell Biology 2024Quote: ... An additional 8 µl of cell lysate was transferred to a 384-well LV plate and ATP was measured by adding 8 µl CellTiter-Glo® Luminescent Cell Viability Assay (Promega Cat. #G7571).
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bioRxiv - Immunology 2024Quote: Cytokine or chemical inhibitor doses were selected based on available EC50 data and toxicity profiles with untreated macrophages (Trypan Blue staining or MTS assay; CellTiter 96 Aqueous One Solution Cell Proliferation Assay Promega, Madison, WI, USA). Only inhibitor doses resulting in >90% cell viability were utilized ...
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bioRxiv - Cancer Biology 2024Quote: ... Luciferase activities were conducted 24 hours after transfection in the cells treated with or without the TEAD inhibitor CP1 using the dual-luciferase reporter kit (cat. E1910, Promega, Madison, WI, USA). Assays were conducted in triplicates and quantified using PerkinElmer EnVision plate reader ...
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bioRxiv - Microbiology 2024Quote: Supernatants from non-infected and infected cell cultures were harvested from all the incubation time points and used for determination of the percentage of cell survival using LDH-Glo™ Cytotoxicity Assay (Promega – cat #J2381) kit following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... 2 hours after bizine treatment cells were exposed to 250μM or 500μM hydrogen peroxide for 2 min after which glutathione (GSH) levels in cells were assayed according to the manufacturer’s instructions for GSH-Glo™ Glutathione Assay (Promega, Madison, WI, USA).
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bioRxiv - Bioengineering 2022Quote: ... was performed to measure the specific killing activity of anti-HIV CAR-T cells toward LHL2/3 cells and LEL6 cells at different ratios from 1:1 to 10:1 by using the CytoTox 96 non-radioactive cytotoxicity kit (Promega, Madison, WI, United States) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: Viability upon treatment with the drugs employed was measured through the CellTiter 96® Non-Radioactive Cell Proliferation Assay (MTT) assay (Promega; Madison, WI, USA) similarly to (Shytaj et al ...
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bioRxiv - Microbiology 2021Quote: ... Viral supernatant was then used to transduce each cell line of interest and readout measured by Luciferase activity 48 hours later (Promega Steady-Glo® (E2550)).
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... COS-7 cells were plated on 10 cm plates and transfected 24h prior to lysis using FuGENE 6 (Promega; 6-12 µg total DNA). For siRNA tests ...
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bioRxiv - Cancer Biology 2020Quote: Molecular fingerprinting for PDOXs and cell lines was performed with Promega PowerPlex® 16 HS or PowerPlex Fusion® System (Promega Corporation, Madison, WI).
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bioRxiv - Pathology 2020Quote: Messenger RNA was isolated from cells or slices (three slices per sample, pooled, homogenized prior to extraction) using Maxwell® LEV Simply RNA Cells/Tissue kit (Promega, Madison, Wisconsin, US). A NanoDrop® ND-1000 Spectrophotometer (Thermo Scientific ...
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bioRxiv - Physiology 2020Quote: ... were isolated from cycling Pdgfrb-BAC-eGFP mice by FACS and RNA extracted using the Maxwell® SimplyRNA cells kit (Promega, Cat. No. AS1270). cDNA was amplified using the NuGEN Ovation V2 system and all purified samples were judged to be of high quality and sufficient quantity for next generation mRNA sequencing using the LabChip method (Perkin Elmer) ...
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bioRxiv - Immunology 2020Quote: Cell viability upon treatment with auranofin and/or BSO was measured using the CellTiter 96®Non□Radioactive Cell Proliferation Assay (MTT) (Promega; Madison, WI, USA) according to the manufacturer’s instructions ...