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Citations for Promega :
5401 - 5450 of 5567 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: TdT-mediated dUTP nick-end labeling (TUNEL) or EdU assays were performed by using in situ cell death detection kit (TUNEL, Promega Corporation, G3250) or EdU Click-iT® Assay Kit obtained from Thermo Fisher (C10639) ...
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bioRxiv - Cancer Biology 2020Quote: ... The membrane was then carefully cut and the numbers of PBMCs that had migrated towards the bottom chamber were quantified using a Cell Titer Glo assay (Promega, Madison, WI) using a standard curve to calculate cell numbers.
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bioRxiv - Cell Biology 2021Quote: ... Briefly 70-90% confluent cells in 6-well plated were transfected with 1 µg CRISPR/Cas9 plasmids per well by using FuGENE 6 (Promega, Madison, Wisconsin). After 24 h post-transfection ...
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bioRxiv - Biochemistry 2020Quote: ... The mutation was confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Microbiology 2021Quote: Cytotoxicity in Huh7 knock-out cell lines and upon inhibitor treatment of Huh7 and VeroE6 cell lines was monitored using CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). Relative cytotoxicity compared to lysed control cells was analyzed ...
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bioRxiv - Biophysics 2021Quote: ... DNA constructs of full-length wt and T654A EGFR (1 μg) were transiently transfected into CHO-K1 cells using FuGENE HD Transfection Reagent (Promega, Madison, WI). For single-molecule measurements ...
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bioRxiv - Microbiology 2022Quote: ... subjected to a freeze-thaw cycle to lyse cells and luciferase activity was measured using the Luciferase Assay System (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: Apoptosis induction in response to SRRM1 silencing in leukemia cell lines (25,000 cells/well onto white-walled multiwell luminometer plates) was performed by using Caspase-Glo® 3/7 Assay (Promega Corporation, #G8091) as previously reported (67) ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was extracted from approximately 106 cells via the Maxwell 16 LEV simply RNA Tissue Kit (Cat. #AS1280, Promega, Madison, WI) on a Maxwell 16 Instrument (Cat ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was assessed using the 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) CellTiter 96 Aqueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... The cell survival ratio was determined by quantifying the reduction in growth compared with mock-treated cells at 48 hours using a standard MTT colorimetric assay (Promega AQueous One). The assay was repeated four times.
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bioRxiv - Bioengineering 2019Quote: ... gels were homogenized in lysis buffer using a hand grinder and were measured using the CellTiter-Glo luminescent Cell Viability Assay (Promega, United States) and the Quant-iT PicoGreen dsDNA assay (ThermoFisher ...
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bioRxiv - Microbiology 2019Quote: ... antibody-dependent cell-mediated cytotoxicity (ADCC) bioassays (29) were performed as prescribed by the manufacturer (ADCC reporter bioassay, complete kit, Promega, cat # G7010). In brief ...
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Measuring adaptation dynamics to hydrogen peroxide in single human cells using fluorescent reportersbioRxiv - Cell Biology 2020Quote: ... Wild-type MCF7 cells (MCF7 WT) were transfected with a plasmid expressing the sensor of interest using FuGENE HD transfection reagent (Promega, Charbonnières, France) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were incubated with chemical treatments for 18 hours at 37°C prior to rinsing with PBS and lysing with 1x cell lysis buffer (Promega, Madison, WI). Luciferase activity was measured using a Berthold Centro XS3 LB 960 microplate luminometer with automatic injection of Luciferase Assay Reagent (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 10×106 HEK293T/17 cells were transfected with a proviral backbone plasmid and an envelope expression vector by using FuGENE6 (Promega, Madison, WI) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... HEK293 cells were transfected with a PPRE-firefly luciferase reporter plasmid together with phRL-TK Renilla luciferase control vector (Promega, Madison, WI), pCMX-PPARα ...
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Fatty acid binding proteins shape the cellular response to activation of the glucocorticoid receptorbioRxiv - Pharmacology and Toxicology 2021Quote: ... Media samples were taken to detect the secreted alkaline phosphatase reporter (GRE-SEAP) before the cells were lysed and assayed for β-galactosidase activity using the β-galactosidase enzyme assay system (Promega) to correct for differences in transfection efficiency ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: To determine Caspase 3/7 activity in THP-1 SAMHD1 KO and CTRL cells the Caspase-Glo 3/7 assay (Promega, Walldorf, Germany) was used according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: The effect of neutralization capacity of Multivalent DARPin was evaluated by exposing serial dilutions of the DARPin candidates to increasing titers of SARS-CoV-2 and determining cell protection by CellTiter-Glo assay (Promega, Madison, USA). Serial dilution of DARPin candidates were prepared in 96 well plates in 100 µl cell culture medium (2%-FBS-MEM + HSA ...
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bioRxiv - Cell Biology 2020Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection® Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
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bioRxiv - Microbiology 2021Quote: ... Luciferase activity in the infected cells was measured by removing the culture supernatant and adding 50 μL Luciferase assay reagent (Firefly luciferase reagent, Promega. Madison, WI). Luminescence was recorded using the luminescence plate reader (Clariostar plate reader ...
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bioRxiv - Biochemistry 2021Quote: ... 20 μl of the cell lysate was used for luciferase reading using 50 μl of Renilla luciferase reagent (Promega, Madison, WI, USA). Relative light units (RLU ...
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bioRxiv - Cancer Biology 2021Quote: ... Plasmids were packaged with the third-generation lentiviral packaging system (VSV-G, pMDLg, and pRSV-REV) and expressed in HEK293T cells via transient transfection with FuGENE 6 (Promega, Madison, WI). Virus-containing supernatants were harvested after 24 and 48 hours ...
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bioRxiv - Cancer Biology 2021Quote: ... with target sequence inserts were cotransfected into HEK293T and MCF7 cell lines with 200 ng of Control/YAP1-S127A construct and 10 ng of Renilla luciferase pRL-TK plasmid (Promega, Cat. #E2241) using Lipofectamine®2000 transfection reagent (Thermofisher ...
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bioRxiv - Microbiology 2021Quote: ... Primary human airway epithelial cells were homogenized in homogenization solution supplemented with 1-thioglycerol and lysed by addition of lysis buffer (Maxwell 16 LEV simplyRNA cells kit; Promega #AS 1270). Prior to RNA extraction ...
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bioRxiv - Immunology 2021Quote: ... The mutation was confirmed by full-length spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega, Catalog #E2692). Pseudovirions for 293T/ACE2 infection were produced by co-transfection with a lentiviral backbone (pCMV ΔR8.2 ...
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bioRxiv - Immunology 2021Quote: ... All mutations were confirmed by full-length Spike gene sequencing. Pseudovirions were produced in HEK 293T/17 cells (ATCC cat. no. CRL-11268) by transfection using Fugene 6 (Promega Cat#E2692) and a combination of Spike plasmid ...
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bioRxiv - Microbiology 2021Quote: ... was performed using the CellTiter 96® AQueous One Solution Cell Proliferation Assay kit using manufacturer’s instructions from Promega (Madison, WI, USA). Vero E6 cells were seeded in 96-well plates at a density of 10,000 cells/well ...
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus titers were measured by infecting different cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (51) ...
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bioRxiv - Immunology 2021Quote: ... H1299 cells were incubated with the pseudovirus for 24 h followed by luciferase assays performed using NanoGlo Luciferase Assay System (Promega, Madison, WI). In one set of experiments ...
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bioRxiv - Developmental Biology 2021Quote: ... in media containing 1% FBS and tested for expression of luciferase using the ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega #E7120). Luciferase expression was measured using a Flexstation 3 plate reader (Molecular Devices) ...
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bioRxiv - Cancer Biology 2021Quote: ... and viability of the spheroids was determined after 7 days of treatment with the CellTiter-Glo® 3D Cell Viability Assay (Promega G9682) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the cells were collected and assayed for Firefly and Renilla Luciferase activities using the Dual-Luciferase Reporter Assay System (Promega, WI, USA) and measured in a Genius luminometer (TECAN ...
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bioRxiv - Molecular Biology 2019Quote: All sorted cell fractions as well as unsorted mixture samples were extracted manually with the DNA IQ™ System (Promega, Madison, WI) following the Virginia Department of Forensic Science’s protocol (17) ...
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bioRxiv - Microbiology 2022Quote: ... 100 μl of these monocultures was added to the wells of an opaqued walled 96 well plate and ATP was measured using a bioluminescent kit as recommended by the manufacturer (BacTiter-Glo Microbial Cell Viability Assay, Promega, Madison, WI). An ATP standard curve was prepared using purified ATP (Sigma Aldrich) ...
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bioRxiv - Cancer Biology 2022Quote: ... 1000 and 5000 µM) or remained untreated for 6 days and viability was analyzed using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Dübendorf, Switzerland) to determine the adenosine triphosphate (ATP ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were incubated for an additional 2 – 6 days and cell growth was quantified using CellTiter-Glo® 2.0 Reagent (Promega, Cat. #924C). For immunoblot analysis ...
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bioRxiv - Cancer Biology 2022Quote: ... Impact of treatments on intracellular ATP content was measured using the CellTiter-Glo® 3D Cell Viability Assay (Promega, cat. no. G9681) at 48 hours (Erastin/YM155 ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were seeded on poly-lysin coated coverslips at a concentration of 0.75 × 105 cells/ml and transiently transfected using the FuGENE® HD Transfection Reagent (Promega, Madison, WI) 28–48 h before measurement.
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bioRxiv - Neuroscience 2024Quote: ... the transfected cells on a 96-well plate were measured for the luciferase activities using Dual-Glo® Luciferase Assay System (Promega, E2920), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ATP levels and the NADH/NAD+ ratio were determined using the BacTiter-Glo™ Microbial cell Viability assay or the NAD/NADH-Glo™ assay (Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and incubated at room temperature with reagent for determination of ATP using BacTiter-Glo Microbial Cell Viability Assay (cat. no. G8232; Promega, Madison, WI), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: BAC-derived MCMV was reconstituted by transfection of BAC DNA into NIH-3T3 cells (ATCC CRL-1658) using FuGENE HD transfection reagent (Promega, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 6 μg of each vector was transfected into tumor cells plated on a 6-well plate using FuGENE6 transfection reagent (Promega, cat. E2691) and OPTI-MEM (GIBCO ...
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bioRxiv - Biochemistry 2024Quote: ... containing NanoLuc substrate was added to the living cells (40 μl/well, containing 1.0 μl of NanoLuc substrate stock from Promega, Madison, WI, USA). Thereafter ...
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bioRxiv - Cancer Biology 2024Quote: ... measurements 1×105 T cells were cultured in white 96 well plate and analysed using NAD/NADH-Glo™ luminescent assay (Promega, G9071), according to the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 cells were seeded in 24-well plates at a density of 4 × 104 or 8 × 104 cells/well and co-transfected 24 hours later with 200 ng of the PsiCheck2 plasmid (Promega, cat #C8021) with the human dystrophin 3′ UTR or mutant 3′ UTR and with 50 nM miR-146a mimic (Life Technologies ...
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Mucociliary Clearance Augmenting Drugs Block SARS-Cov-2 Replication in Human Airway Epithelial CellsbioRxiv - Microbiology 2023Quote: ... The plates were incubated at 37°C under 5% CO2 for 144 hour and virus cytopathic effect was measured using the CellTiter-Glo® 2.0 Cell Viability Assay (Promega, Cat. # G9241) following the manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from 1×106 cryopreserved lymph node (LN) mononuclear cells (LNMC) isolated from LN biopsies using the SimplyRNA Tissue kit for the Maxwell RSC instrument (Promega, Madison, WI). RNA was then tested for both SIV gag and a cellular gene ...