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Citations for BioLegend :
1201 - 1221 of 1221 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Serum interferon alpha and beta were analyzed by ELISA (LEGEND MAX™ Mouse IFN-β ELISA Kit – BioLegend) (ELISA MAX™ Deluxe Set Mouse IFN-α1 – BioLegend).
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bioRxiv - Immunology 2024Quote: Concentrations of IFN-γ in mouse plasma and ex vivo stimulation supernatants were determined using the ELISA MAX Deluxe Set Human IFN-γ Kit (BioLegend, cat #430104) according to the manufacturer’s recommendations ...
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bioRxiv - Immunology 2024Quote: ... CD8 T cells from the CNS were enriched from brain cell pellets post Percoll gradient centrifugation using MojoSort Mouse CD8 T Cell Isolation Kit (BioLegend, cat. no. 480035). For Cre- and Cre+ mice ...
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bioRxiv - Immunology 2024Quote: ... and Propidium Iodide (PI) (1 μg/ml) according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI, BioLegend, San Diego, CA, USA). The staining of Annexin V requires the usage of a binding buffer for Annexin V as a staining buffer ...
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bioRxiv - Immunology 2021Quote: ... were determined using bead based immunoassays (CBA kit, Cat. 560484, BD PharMingen, San Diego, CA, USA; and LEGENDplex, Cat. 740003, BioLegend, San Diego, CA, USA). Log-transformed data were used to construct standard curves fitted to 10 discrete points using a 4-parameter logistic model ...
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bioRxiv - Cancer Biology 2021Quote: ... Further preparation for FACS measurement was following the protocol of the BioLegend APC Annexin V Apoptosis Detection Kit and DNA-staining with DAPI Reagent (25 μg/mL) (Biolegend, San Diego, CA, USA). 20 000 cells were assayed using a flow cytometer FACSCantoII (Becton Dickinson ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were resuspended in PBS with 2% FBS containing anti-mouse CD16/CD32 antibody (table 1) and 1:1000 Fixable Viability Dye eFluor™ 506 (eBioscience, Cat#65-0866) or Zombie UV™ Fixable Viability Kit (BioLegend, Cat#423108). Staining with primary fluorophore-conjugated antibodies directed against cell surface markers (Additional file 4 ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with DPBS and harvested with trypsin-EDTA and the viability analyzed by flow cytometry using the Apoptosis Detection Kit with propidium iodide (PI, BioLegend, San Diego, CA, USA) in a Cytoflex flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: Flow cytometry viability staining was conducted by suspending murine spleen and tumor single cell suspensions in Zombie Violet fixable viability kit (Cat # 423114, BioLegend, San Diego, CA, USA) according to manufacturer instructions for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... Transduced T cells were positively selected with a PE selection kit (EasySep, ref: 17684) and an anti-HA PE-conjugated antibody (Biolegend, ref: 901518, clone: 16B12) at a 1:50 dilution to enrich transduced T cells ...
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bioRxiv - Immunology 2023Quote: Plasma and culture supernatants were collected and frozen at -80°C until cytokines measurement by commercial ELISA kits (human IL-1ß and IL-18 R&D Systems; human TNF-α and IFN-γ, Biolegend).
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bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Genomics 2023Quote: ... Test wells and the Zombie-only control wells were incubated with Zombie Violet Fixable Dye diluted in PBS (Zombie Violet™ Fixable Viability Kit (423113, BioLegend, San Diego, CA, USA) for 15 min at 4°C in the dark ...
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bioRxiv - Developmental Biology 2021Quote: ... then stained for one hour at 4°C in the dark with a mix of rat anti-mouse CD117/c-kit (labelled with PE) (BioLegend – 105808; 0.4 µg per million cells), rat anti-mouse CD34 (labelled with AF700 ...
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bioRxiv - Cancer Biology 2022Quote: ... and double knockdown cells were treated with the IC50 value of cisplatin and viability was assessed via Annexin V and propidium iodide staining by flow cytometry (Biolegend APC Annexin V Apoptosis Detection Kit).
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 900 x g for 5 minutes at room temperature and stained with Zombie Aqua Fixable viability kit (Biolegend, 1:1000 diluted in PBS, 100µL) for 20 minutes at room temperature in the dark to enable exclusion of dead cells ...
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bioRxiv - Immunology 2024Quote: ... was sorted on the BD FACS Symphony S6 5L after excluding the dead cells by using a Fixable Viability Kit (Zombie Nir™, Biolegend, Cat# 423105, dilution 1:400) and the stromal cell populations (TER-119−CD31−CD45−) ...