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Citations for BioLegend :
851 - 900 of 1221 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... A panel of 13 cytokines was quantified with a bead-based ELISA kit (BioLegend, San Diego, CA) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... Live-dead cell discrimination was performed using Zombie Aqua™ Fixable Viability Kit (1:1000, 423102, Biolegend) according to manufacturer instructions ...
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bioRxiv - Immunology 2024Quote: Single-cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kits (Biolegend®), followed by cell surface staining and measurements using a BD LSRFortessa™ or LSRII high-parameter cell analyzer ...
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bioRxiv - Microbiology 2024Quote: ... and growth factors measurement using LegendPlex bead-based multiplex technology and NHP inflammation 13-plex kits (BioLegend). Serum samples were processed in duplicate at a 1:4 dilution according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... splenic B cells were isolated according to the MojoSort™ Mouse Pan B Cell Isolation Kit (BioLegend) protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Following stimulation cells were collected and fixed with a Cyto-Fast fixation and permeabilization kit (Biolegend, 426803) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: Mouse IL-10 and IL-1β proteins were measured in cell culture supernatants using kits from Biolegend and Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Secreted IL-2 in the supernatant was measured using Human or Mouse IL-2 ELISA Kit (BioLegend). All incubations were performed at 37 °C / 5% CO2.
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bioRxiv - Immunology 2024Quote: ... Naïve CD8+ T cells were then enriched using Mojosort mouse CD8+ naïve T cell isolation kit (Biolegend). Purified naïve CD8+ T cells were resuspended in RPMI media supplemented with 5% FBS ...
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bioRxiv - Immunology 2024Quote: ... the amount of IL-2 in the medium was quantified using Human IL-2 ELISA kit (Biolegend) or CD69 expression on Jurkat cells was measured by FACS ...
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bioRxiv - Cancer Biology 2021Quote: ... MHCll-APC/Cy7 (clone M5/114.15.2) and Live/Dead Zombie UVTM Fixable viability kit were purchased from Biolegend. All antibodies were tested with their isotype controls ...
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bioRxiv - Immunology 2021Quote: ... Staining for FOXP3 followed the instruction provided by True-Nuclear Transcription Factor Buffer Set kit (BioLegend, Cat#: 424401). For apoptosis analysis ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from bone marrow chimeras (MojoSort™ Mouse Pan B Cell Isolation Kit; Biolegend). Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC ...
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bioRxiv - Developmental Biology 2020Quote: ... and labeled using a FITC Annexin V and propidium iodide staining kit as per manufacturer’s protocol (BioLegend, 640914). Both EdU- and Annexin V-labeled samples were analyzed on a LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... and about 2×105 cells were resuspended in 100μl binding buffer (FITC Annexin V Apoptosis Detection Kit, Biolegend). Subsequently ...
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bioRxiv - Immunology 2021Quote: IL-21 concentration from tonsil mono-cultures was measured using Human IL-21 ELISA MAX Deluxe kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2021Quote: The Annexin V staining was performed using the FITC Annexin V apoptosis detection kit with PI (BioLegend, USA). The cells were washed with PBS followed by washing with staining buffer and resuspension in binding buffer containing anti-Annexin V and PI and incubation for fifteen minutes at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed with 1xPBS and fixed using buffer in True-Nuclear Transcription Factor Staining kit (Biolegend). After fixing ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence labeled antibodies (CD209,CD206 et al) and multi cytokines kit were from Biolegend (San Diego, CA, USA). Specific inhibitors were acquired from MedChemExpress (Monmouth Junction ...
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bioRxiv - Systems Biology 2021Quote: ... The levels of interleukin (IL)-6 and tumor necrosis factor (TNF)-α were detected using ELISA kits (Biolegend) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2020Quote: ... fixed and permeabilized using BD Cytofix/Cytoperm kit and then stained for IL-10 expression (JES3-19F1, Biolegend). Flow cytometry was performed on LSR Fortessa (BD Biosciences ...
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bioRxiv - Bioengineering 2022Quote: ... IFNγ concentrations were determined via standard curve using a commercial ELISA kit according to the manufacturer’s instructions (Biolegend). Absorbance was measured on a multi-mode plate reader (Tecan).
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (31,50).
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bioRxiv - Cell Biology 2021Quote: ... After a quick wash with DPBS cells were stained with Zombie Red™ Fixable Viability Kit (#423110, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Apoptotic analysis was determined using the FITC Annexin V apoptosis detection kit with PI (Propidium Iodide) (Biolegend 640914) and performed according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with the appropriate surface markers [dead cell marker-Zombie Aqua™ Fixable Viability Kit (Biolegend)] and CD8 (APC conjugated ...
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bioRxiv - Immunology 2021Quote: ... Lung cytokine levels were assessed from the supernatant samples via LEGENDplex kit (mouse inflammation panel 13-plex; BioLegend) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were isolated from spleens and lymph nodes of BDC-2.5 transgenic NOD mice using the Mouse CD4 Naïve T Cell Isolation Kit (BioLegend). Naïve T cells were further selected positively by treatment with anti-CD62L biotinylated antibodies (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... Sera were assayed for cytokines using a 13-plex LEGENDplex Mouse Inflammation Panel kit (Biolegend, San Diego, CA) following the manufacturer’s instructions using an Accuri C6 flow cytometer (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... Cell death was analysed 18h later by FITC Annexin V/PI Apoptosis Detection Kit with PI (640914; Biolegend).
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: ... The concentration of CXCL10 in the media was determined using a Human CXCL10 ELISA MAX kit (BioLegend 439904).
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Systems Biology 2023Quote: ... Dead cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) or Zombie Violet Fixable Viability Kit (BioLegend). Cells were stained with the following fluorochrome-conjugated anti-human Abs ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Immunology 2022Quote: ... Twelve days post-treatment tumors were dissociated and subjected to CD45.1 positive selection (MojoSort mouse CD45.1 selection kit, Biolegend) per the manufacterer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution (pH 7.2S ...
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bioRxiv - Immunology 2023Quote: TNFα and CCL5/RANTES were quantified in cell culture supernatants using mouse ELISA kits (BioLegend, R&D Systems) according to manufacturers’ instructions ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Release of IL-6 and IL-8 were measured using commercial ELISA kits (Biolegend, San Diego, CA, USA) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Supernatant were collected and measured by ELISA kits according to the manufacturer’s instructions (catalog 438707, 440507, 431414; BioLegend). Absorbance was measured at 450 nm with a microplate reader (Molecular Devices).
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
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bioRxiv - Immunology 2023Quote: ... The amount of secreted IFN-γ was determined the LEGEND MAX™ Human IFN-γ ELISA Kit (BioLegend) according to the manufacturer’s instructions.