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Citations for BioLegend :
951 - 1000 of 1221 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202 ...
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bioRxiv - Cancer Biology 2021Quote: Trypsinized and washed Panc02 cells were stained with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend) and analyzed using the CytoflexS Flow Cytometer (Beckman Coulter).
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bioRxiv - Immunology 2020Quote: CD4+ T cells were harvested from the lymph nodes and spleens of naive NR1 mice and enriched with a mouse naïve CD4 negative isolation kit (BioLegend) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... cells were fixed and permeabilized with a Transcription Factor Staining Kit (eBioscience) and stained using anti-IFNγ (XMG1.2; 200x; BioLegend) and recombinant mouse IL21R Fc Chimera (600x ...
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bioRxiv - Immunology 2020Quote: CD8 T cells were isolated from an OT1+ mouse using the mojosort mouse CD8 T cell isolation kit (Biolegend) and labeled with violet proliferation dye (BD Biosciences cat# 562158) ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were isolated from spleen using MojoSort™ Mouse CD3 T Cell Isolation Kit (Biolegend Cat no; 480024) as per the manufacturers protocol ...
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bioRxiv - Immunology 2021Quote: For differentiation of live and dead cells we stained cells with the Zombie Violet Fixable Viability Kit according to the manufacturer’s instructions (BioLegend). For surface marker analysis ...
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were first stained with viability stain (Zombie NIR fixable viability kit, BioLegend, San Diego, California, USA) followed by either BV510-αCD45 (clone ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated from the spleen using Mojosort Magnetic Separation System CD8 Naïve T cell Isolation Kit (BioLegend). Anti-CD3ε Ab (clone 145-2111C ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was harvested and cytokines were quantified using a Legendplex Mix and Match Kit according to manufacturer instructions (Biolegend).
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Bioengineering 2023Quote: Lung single-cell suspensions were stained for 30 min at RT with Zombie Red™ Fixable Viability Kit (BioLegend) and were blocked with anti-mouse CD16/32 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... tissues were blocked for endogenous avidin and biotin using an avidin biotin blocking kit according to the manufacturer’s instructions (BioLegend). Sections were then incubated with biotin conjugated goat anti-chicken (Vector Labs ...
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bioRxiv - Neuroscience 2023Quote: ... Single cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution pH 7.2 (PBS ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Immunology 2023Quote: Appropriate ELISA kits were used to quantify human or murine IL-6 and/or TNF concentrations according to manufacturer’s recommendations (Biolegend). The 450 nm absorbance was measured by a SpectraMax® plate reader.
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bioRxiv - Microbiology 2022Quote: ... Apoptotic cells were detected using the PE Annexin V Apoptosis Detection Kit with 7-AAD according to the manufacturer’s protocol (BioLegend) and analyzed using an LSRII flow cytometer.
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bioRxiv - Cancer Biology 2024Quote: ... IFN-γ secretion to validate CAR-T cell activation was measured using the Human INF-γ ELISA kit (Biolegend) or Human IFN-γ DuoSet ELISA kit (R&D Systems) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and labeled with propidium iodide (PI) and Annexin-V-FITC (Annexin V FITC Apoptosis Detection Kit with PI, Biolegend) for the detection of necrosis and apoptosis ...
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bioRxiv - Immunology 2024Quote: ... Naïve CD4+ T cells isolated from the mouse spleen using the MojoSort Mouse Naïve CD4 T Cell Isolation Kit (#480040, BioLegend) were stimulated in a plate coated with LEAF purified anti-mouse CD3ε antibody (clone 145-2111C ...
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bioRxiv - Immunology 2024Quote: ... coupled with the protocol TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 or v3.1 (Single Index) from BioLegend.
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bioRxiv - Immunology 2023Quote: ... CD4+ OT-II KN2 cells were purified from CD45.1.2 OT-II KN2 donor mice with the MojoSort™ Mouse CD4 T Cell Isolation Kit (Biolegend) and labeled with cell proliferation dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were purified from spleens and lymph nodes using MojoSort™ CD4 isolation kit and magnet (Biolegend). Purities of cells were determined for each experiment using flow cytometry ...
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bioRxiv - Cell Biology 2023Quote: ... The cell pellet was resuspended in 50 µl of the Annexin V Apoptosis Detection Kit/7-AAD solution (BioLegend) and incubated for 15 min ...
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bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated from the spleen by using the MojoSort Mouse Naïve CD4+ T Cell Isolation Kit (#480040, BioLegend). CD4+ T cells isolated from the OT-II spleen were cocultured with BMDCs ...
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bioRxiv - Immunology 2022Quote: ... cells were washed again using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Biolegend; 423101) for 20 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: Cytokine concentration of the basolateral compartments were measured using the human antivirus response panel (13-plex) kit (LEGENDplex BioLegend). The kit was used according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2023Quote: Single-cell suspensions from tumors or CD3 T cells isolated using MojoSort Mouse CD3 T Cell Isolation Kit (Biolegend) following the manufacture’s protocols ...
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bioRxiv - Immunology 2023Quote: ... cells treated with a Foxp3/Transcription factor staining buffer kit (Cat. TNB-0607, TOMBO Bioscience) were stained with FITC-labeled anti-mouse CD4 Ab (GK1.5, BioLegend) and allophycocyanin-labeled anti-mouse Foxp3 Ab (3G3 ...
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bioRxiv - Immunology 2023Quote: ... and IL-22 were measured in the culture supernatants using the LEGENDPlex Mouse T Helper Cytokine Panel kit (BioLegend) following manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... The last four dilutions were used for ELISA with Legend Max Human alpha-synuclein (colormetric) ELISA kit (Biolegend Inc.) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Human CD4+ T lymphocytes were isolated from PBMCs using the MojoSort Human CD4+ T Cell Isolation Kit (BioLegend, 480010) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The amount of IL-2 in the culture medium was measured using a mouse IL-2 ELISA kit (Biolegend).
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bioRxiv - Genomics 2024Quote: Resuspend the isolated PBMCs in the recommended buffer provided with the MojoSort Human CD4 T Cell Isolation Kit (Biolegend). Add a biotin-conjugated antibody cocktail specific for non-CD4+ cells (e.g. ...
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bioRxiv - Immunology 2024Quote: Naive CD4+ T cells were isolated from mouse spleens by negative selection using MojoSort Mouse CD4 Naïve T Cell Isolation Kits (Biolegend). Plates were coated with 3µg/ml anti-CD3 and 3 µg/ml of anti-CD28 antibody (Biolegend clones 145-2C11 and 37.51 ...
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bioRxiv - Microbiology 2024Quote: ... then stained for extracellular marks (as above) and then fixed and permeabilized with Cyto-Fast Fix/Perm kit (BioLegend) or Mouse Foxp3 fixation and permeabilization solutions (BD Bioscience ...
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bioRxiv - Immunology 2024Quote: ... and CD3 or CD8 cells were isolated negative selection following the protocol of MojoSort kits (BioLegend 480024; BioLengend 480008). Isolated mouse T cells were cultured in T cell media ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with a cocktail of antibodies and the Zombie NIR fixable viability kit (1:1000, Biolegend, 423105) prepared in PBS for 20 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... anti-CD16/CD32 (93) and Zombie Aqua™ Fixable Viability Kit were purchased from BioLegend (San Diego, CA, USA). Recombinant murine granulocyte macrophage-colony stimulating factor (rmGM-CSF ...
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bioRxiv - Immunology 2024Quote: ... CXCL10 in supernatant was measured using the ELISA MAX™ Deluxe Set Human CXCL10 (IP-10) kit (Biolegend, 439904). OD450nm and OD570 as background were measured using Multiskan SkyHigh plate reader (Thermo ScientificTM).
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the blood of MMTV-PyMT tumour bearing mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Neuroscience 2024Quote: Cytokine levels in supernatant collected from BALF or cell culture were determined with commercially available ELISA kits from Biolegend, R&D systems ...
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bioRxiv - Biochemistry 2024Quote: ... We determined the amounts of IL-2 in the culture supernatant of activated or naïve CD4+ T cells with an ELISA kit (BioLegend). We measured the amounts of IgE in the serum with a commercially available ELISA kit (Sigma-Aldrich).
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bioRxiv - Immunology 2024Quote: Skin cytokines or growth factors were quantified using LegendPlex Mouse Cytokine release syndrome and Hematopoietic stem cell kits (Biolegend). Briefly ...
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bioRxiv - Immunology 2024Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, # 423107)) and incubated on ice for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: Neutrophils were isolated from the femurs of MMTV-PyMT tumour bearing mice using MojoSort Mouse Neutrophil Isolation Kit (Biolegend), with neutrophil retention via negative selection strategy ...
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bioRxiv - Immunology 2024Quote: ... The first block was for 30 min with endogenous biotin and avidin with an Avidin/Biotin Blocking Kit (BioLegend) and the second block was for 1 hr with 1× TBS IHC Wash Buffer with Tween 20 with 3% (v/v ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained for surface markers and then prepared for intracellular staining using True-Nuclear Transcription Factor staining kits (Biolegend). For experiments demonstrating stimulation response ...