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Citations for BioLegend :
1151 - 1200 of 1221 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... the following fluorophore-conjugated antibodies were used at the indicated dilutions: CD117 (c-Kit) BV785 (clone 2B8, BioLegend; 1:200 dilution), Ly-6A/E (Sca-1 ...
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bioRxiv - Cancer Biology 2024Quote: ... KRASG12D and KRASG12D/C185D were stained with FITC-Annexin V and 7-AAD following the instruction of the FITC-Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend; # 640922) and the samples were run on a BD LSRFortessa cell analyzer.
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bioRxiv - Cancer Biology 2024Quote: Spleen was isolated from C57BL/6 mice and CD8 T cells were isolated using MojoSort™ Mouse CD8 T Cell Isolation Kit (Biolegend). The cells were stimulated with Dynabeads™ Mouse T-Activator CD3/CD8 kit (Gibco) ...
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bioRxiv - Cancer Biology 2024Quote: ... on 96-well conical bottom plate and incubated for 15 min in dark at RT in 50 µL of Zombie NIR™ Fixable Viability Kit (Biolegend). After that ...
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bioRxiv - Immunology 2021Quote: The IFN-β levels in cell supernatants and mouse sera were measured with ELISA kits purchased from BioLegend (San Diego, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2020Quote: ... KC and IL-6 in the cell culture supernatants and lung homogenates were determined by commercialized ELISA kit (IL-1β and KC were from R&D Systems BD, IL-6 was from Biolegend) and SAA3 in the lung homogenates and plasma were determined by ELISA kit (MilliporeSigma ...
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bioRxiv - Neuroscience 2021Quote: ... assays were performed using the in situ red starter kit according to the manufacture’s protocol with JADE1 antisera (1:20) and 0N tau antisera (1:500; BioLegend, San Diego, CA). Two control assays were also performed ...
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bioRxiv - Cell Biology 2021Quote: ... Dead cell compensation and gating was completed using a 50 % mixture of live and dead mESCs stained with Zombie Aqua™ Fixable Viability Kit (BioLegend 423101) and incubated in the light-tight container at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... naïve CD4+ T cells were isolated from the spleens of C57BL/6 mice or IL17-GFP mice using a MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend cat. 480040). T cells from C57BL/6 mice were further labeled with CellTrace™ Violet (Invitrogen cat ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.
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bioRxiv - Cancer Biology 2021Quote: ... and early/late apoptosis was measured by using the PE Annexin V apoptosis detection kit with 7-AAD (Biolegend, San Diego, USA) according to the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: ... Triplicate wells were cultured in complete media or switched to serum free DMEM for 24 hours before screening with Annexin V APC/PI using the Apoptosis detection kit (Biolegend; cat 640932) according to the manufacturer’s instructions ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... A custom rat multi-analyte LEGENDplex bead-based immunoassay kit was used to examine cytokine concentrations in plasma samples (LEGENDplex; BioLegend, CA, USA) that targeted six cytokines concurrently (IL-6 ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: Cytotoxicity of PE-coated and uncoated MNPs on various DC subsets was evaluated using the Zombie Aqua Viability Kit (BioLegend, Fell, Germany) according to manufacturer’ s protocol ...
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bioRxiv - Immunology 2022Quote: ... The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet™ fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11 ...
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Neuroscience 2023Quote: ... conjugated using Thermo Fisher Alexa Fluor Kits (AF-488 catalog #A20181, AF-555 catalog #A20187, AF-647 catalog #A20186) to PrP (3F4) (BioLegend catalog #800301) or CD47 (MIAP410 ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: CD4+ cells were isolated from pooled splenocytes and lymph node cells with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... density centrifugation or 1X RBC Lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and stained using the Zombie NIR Fixable Viability Kit (Biolegend, 423106, diluted 1:500 in PBS) for 30 min at 4°C prior to Fc blocking and staining of surface antigens ...
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bioRxiv - Immunology 2023Quote: Cytokine production was quantified from cell supernatants or serum using the following ELISA kits: ELISA MAX Deluxe Set Mouse IL-10 (BioLegend, Cat# 431414), ELISA MAX Deluxe Set Human IL-10 (BioLegend ...
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from in vitro treated CAR-T cells using Mojosort™ Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). After washing cells with PBS twice ...
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bioRxiv - Genetics 2023Quote: ... were isolated by 1X Red Blood Cell lysis Buffer (eBioscience) and stained with a combination of fluorochrome-conjugated antibodies: c-Kit (BD Biosciences, BioLegend clone 2B8), Sca-1 (BioLegend clone D7) ...
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bioRxiv - Bioengineering 2023Quote: ... the serum samples collected at 6 hours and 48 hours after administration were diluted 2-fold for ELISA analysis using the LEGEND MAX™ Rat IL-6 ELISA Kit with pre-coated plate (Biolegend, USA) according to the manufacturer’s recommendations with a little modification as described below ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or TRIM5α-HA expression (using the CytoFix/Cytoperm kit [BD] according to manufacturer’s instructions and staining with anti-HA-PE [Biolegend] diluted 1:200). Cells were gated on size (FSC vs ...
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bioRxiv - Immunology 2024Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Zombie Nir™, Biolegend, Cat# 423105, dilution 1:400).
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A resource for extracellular vesicles from activated CD4 T cells that relay pro-inflammatory signalsbioRxiv - Immunology 2024Quote: ... Animals were euthanised using CO2 inhalation and their spleens were aseptically extracted to isolate TH cells employing MojoSort™ Mouse CD4+ T Cell Isolation Kit (Biolegend, #480033). Isolated T cells were cultured in complete RPMI-1640 medium and activated using CD3/CD28 Dynabeads to induce activation ...
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bioRxiv - Bioengineering 2024Quote: Suspended cells prepared as above were stained for live/dead cell quantification with the Zombie NIR Fixable Viability Kit following with the manufacture’s protocol (1:100 dilution, 423111; BioLegend, San Diego, CA). Subsequently ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Immunology 2020Quote: ... We also isolated plasma from GVHD patients and healthy donors and performed cytokine ELISAs on these plasma samples using multiplex ELISA kits (Biolegend, San Diego, CA).
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bioRxiv - Cancer Biology 2021Quote: ... and subjected to the staining of 332 surface antigens using LEGENDScreen Human Cell Screening PE Kit (cat.no. 700001; Biolegend, San Diego, CA, USA). Data acquisition was performed on FACSVerse (Becton Dickinson (BD) ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Physiology 2022Quote: Apoptotic cells were analyzed by flow cytometry using the FITC Annexin V Apoptosis Detection Kit with propidium iodide (BioLegend, San Diego, CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... and proceeded to Annexin V-fluorescein isothiocyanate (FITC)/PI staining according to the manufacturer’s instructions (FITC Annexin V Apoptosis Detection Kit with PI; BioLegend, San Diego, CA, USA). Cells stained with Annexin V (both Annexin V+- and Annexin V+/PI+-cells ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then stained for viability using Zombie NIR™ fixable viability kit for 15 min at room temperature (Cat # BioLegend, CA, USA), followed by fluorescently labeled high- or low-molecular weight HA (2.5mg/ml in PBS ...
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bioRxiv - Physiology 2024Quote: IL-1β concentrations in the conditioned medium from murine splenic cells were measured by ELISA kits (mouse IL-1β, 432604, BioLegend, San Diego, USA) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... naïve CD4 and CD8 T-cells were isolated from syngeneic cryopreserved PBMC aliquots using the MojoSort magnetic negative selection kits (BioLegend, 480041 and 480045). Naïve T-cells were incubated in complete medium supplemented with 10 IU/mL recombinant human IL-2 (R&D Systems ...
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Physiology 2023Quote: CD8+ cells were isolated from the spleens of four 8-week-old diabetic female NY8.3 NOD mice using a MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017 ...
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bioRxiv - Immunology 2023Quote: Cytokine concentrations in supernatants harvested from CAR T cell: target cell co-cultures were determined by ELISA utilizing IFNγ and IL2 Deluxe kits (Biolegend; 431806 and 430106) according to the manufacturer’s instructions.