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Citations for BioLegend :
1101 - 1150 of 1221 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Mouse IL-10 levels were measured using a commercial ELISA quantification kit according to the manufacturer’s instructions (BioLegend, Cat# 431411).
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bioRxiv - Cell Biology 2024Quote: ... and the supernatant was used for ELISA analysis of IL-2 and latent TGF-beta secretion levels by the kits from Biolegend according to manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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bioRxiv - Molecular Biology 2020Quote: ... and LCL A382T TDP-43 mutated patients (LCL-TDP382) were incubated with a vitality dye for 15 minutes (Zombie Violet™ Fixable Viability Kit, BioLegend). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Cancer Biology 2020Quote: ... fixed and stained for intracellular Ki67 following BD Pharmingen’s procedure for FITC BrdU Flow Kit (Cat# 559619) with two modifications: DNase treatment step was omitted and Ki67-AlexaFluor488 (Biolegend #350507) was used instead of anti-BrdU FITC.
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bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Microbiology 2020Quote: Levels of cytokines and soluble mediators present in plasma of infected mice were quantified using LEGENDplex™ Mouse Inflammatory Panel kit (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Microbiology 2020Quote: ... The levels of murine IL-6 and TNF-α and human IL-6 were measured using ELISA MAX™ kits (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2020Quote: Active TGF-β1 serum levels in patients and healthy donors were measured using LEGEND MAX™ Free Active TGF-β1 ELISA Kit with Pre-coated Plates (Biolegend). The assay was run according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Bioengineering 2021Quote: ... and IL-10 were analyzed using a human COVID-19 Cytokine Storm Panel 1 (13-plex) kit (Cat. No. 741091; BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated in 200 μl FACS buffer containing 1 μl Zombie NIR fixable dye for 30 min to determine cell viability (Zombie Green™ Fixable Viability Kit, BioLegend). Cells were fixed for 15 min in 200 μl PFA 2 % ...
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bioRxiv - Immunology 2023Quote: ... Ex vivo CD8+ T cells or in vitro stimulated (IVS) cultures were labeled with the Zombie Aqua™ Fixable Viability Kit (BioLegend) to exclude dead cells in all flow cytometry experiments ...
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Microbiology 2023Quote: ... cells were incubated in 100 µl PBS 1X containing 1.5 μL Zombie NIR Fixable Viability kit (BioLegend, San Diego, CA, USA) at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: OVA-specific IgE levels were measured using a LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit (BioLegend, San Diego, CA). The plate was washed 4 times with wash buffer followed by addition of Matrix A to standard wells and Assay Buffer to sample wells ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Immunology 2023Quote: ... CD3+ CAR-T cells were then purified from lymphocytes using MojoSort™ Mouse CD3 T Cell Isolation Kit according to the manufacturer’s instructions (BioLegend, 480031). CAR-T cells were then activated for 2 days at 1 × 106 cells per 24-well flat-bottomed plates with an equivalent number of CD3/CD28 washed Dynabeads (Gibco ...
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bioRxiv - Developmental Biology 2023Quote: The flow cytometry staining for necrosis and apoptosis was was performed with the FITC Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend, 640922). Once the cells were incubated in 10 µM of EdU ...
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bioRxiv - Immunology 2023Quote: Proliferation of blood ASC and BM LLPC was evaluated on the basis of bromodeoxyuridine (BrdU) incorporation using a phase-flow BrdU commercial kit (BioLegend, Inc.) in accordance with the manufacturer’s recommendations ...
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bioRxiv - Immunology 2023Quote: ... For adoptive transfer and co-culture experiments naïve OT-II cells were magnetically enriched with MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit (BioLegend Cat: 480040) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with PBS buffer and stained with a kit of Zombie Aqua™ Fixable Viability (Biolegend, cat.423101) for 20 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were stained with Annexin V/PI using the Apoptosis Detection Kit according to the manufacturer’s protocol (BioLegend, Cat# 640945). Cells were acquired on a BD LSR Fortessa ...
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bioRxiv - Immunology 2022Quote: ... True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 (clone MF-14) with CD25 and CD4) was used with FOXP3 Fix/Perm Buffer from BioLegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Microbiology 2022Quote: The level of TNF-α protein expression was evaluated after cell transduction with TPA-TNFα in the cultured medium using human TNFα ELISA kit (Biolegend, UK). First ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Twenty four hours after irradiation cells were trypsinized and stained using the FITC Annexin V Apoptosis Detection Kit with PI (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2024Quote: ... Naive CD4+ cells were isolated by negative selection using the MojoSort CD4+ naïve T-cell isolation kit from Biolegend (Catalog 480040) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were negatively selected from the lung single-cell suspensions utilizing the MojoSort™ Mouse CD4 T Cell Isolation Kit (BioLegend) in alignment with the manufacturer’s guidelines.
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bioRxiv - Immunology 2024Quote: Production of IFNγ and IL-10 from T cell culture supernatant was measured using Legend Max ELISA Kit Mouse IFNγ (BioLegend, 430807) and Mouse IL-10 (BioLegend ...
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bioRxiv - Cell Biology 2024Quote: hiPSC-CMs were dissociated into single cells with 0.05% trypsin-EDTA and stained with the fixable Zombie NIR™ Viability Kit (BioLegend®) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... peritoneal lavage and culture medium of primary BMDMs were determined by a LEGENDplex™ bead-based multiplex ELISA kit (Biolegend, USA). The procedures were performed fully according to the product manuals ...
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bioRxiv - Bioengineering 2024Quote: ... supernatants were drawn off the cells and analyzed for cytokine content (i.e., IL-2, IFN-γ, and TNF-α) by ELISA kits (Biolegend).