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Citations for BioLegend :
8451 - 8500 of 8821 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The isolated single-cell suspension and peripheral leukocytes were fluorescently stained with anti-mouse CD11b-FITC (1:200, BioLegend, 101205) and Ly6G-PE (1:80 ...
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bioRxiv - Cancer Biology 2023Quote: ... 90% of the thawed cells (>200 x 106) were stained with biotinylated antibodies against CD45 (1:50, clone HI30; Biolegend) and CD235a (1:50 ...
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bioRxiv - Immunology 2023Quote: ... followed by staining with a premixed cocktail of the indicated tetramers at a final concentration of 10 nM for 1 hour at RT in the presence of anti-mouse CD16/32 antibody (clone 93, BioLegend). SLO preparations were magnetically enriched for tetramer-specific cells as previously described64 while lung samples were magnetically enriched for CD90.2 expression (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... Cell staining followed a protocol developed in-house consisting of two separate panels of antibodies [Panel 1: CD14 (FITC) (#367116 BioLegend), CX3CR1 (PE ...
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bioRxiv - Microbiology 2023Quote: ... 100,000 cells were plated in 96-well round bottom plate and blocked with 1:1000 Human TruStain FcX™ (Biolegend) diluted in washing buffer for 10 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... An aliquot of the resulting suspensions in PBS were immediately stained for viability with Zombie Aqua diluted 1:1000 (BioLegend) for 20 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Molecular Biology 2023Quote: Cells were incubated for 30 minutes at room temperature in 0.5% BSA PBS with 1:50 CD81-FITC antibody (Biolegend, 349504) or mouse IgG1 FITC isotype control antibody (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 1 mM EDTA in HBSS) at 1 x 107 cells/mL and incubated with Fc block (anti-mouse CD16/32; clone 93. BioLegend) for 10 minutes before incubation with specific antibodies listed in Table S1 ...
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bioRxiv - Immunology 2023Quote: Peyer’s patch leukocytes from 10 WT B6 mice de novo colonized for 21 days with SFB were separately stained with oligo-hashtagged antibodies (TotalSeqC 1 – 10, Biolegend), prior to pooling ...
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bioRxiv - Neuroscience 2023Quote: Primary antibodies and dilutions used for western blot were: Mouse Anti-Aβ (6E10 clone, 1:1,000, Biolegend, Cat# 803001, RRID:AB_2564653), goat anti-Mouse DKK-3 (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Neurons were then maintained for 10 min at 37°C in the presence of AlexaFluor-647-conjugated AnxA5 (1/50, Biolegend) in resting solution (Locke’s solution:140 mM NaCl ...
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bioRxiv - Physiology 2023Quote: ... Serum samples were prepared for Legendplex assays by diluting 1:50000 for the detection of immunoglobulin isotypes using the mouse immunoglobulin isotyping kit (BioLegend). The assay was performed in a V-bottom plate and data was acquired on a BD Fortessa X-30 cytometer (BD Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
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bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...
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bioRxiv - Genomics 2023Quote: ... Many of the iPSC lines were evaluated by flow cytometry for expression of two pluripotent markers: Tra-1-81 (Alexa Fluor 488 anti-human, Biolegend) and SSEA-4 (PE anti-human ...
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bioRxiv - Immunology 2024Quote: ... and 2.5 mM HEPES into 6-well plates pre-coated for more than 1 h with 5.4 µg of Ultra-LEAFTM purified anti-mouse CD3e antibody (clone 145-2C11, Biolegend, # 100340) and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51 ...
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bioRxiv - Plant Biology 2024Quote: ... which were then probed with the following primary and secondary antibodies with the indicated dilutions: αHemagglutinin (HA, 1:2000, BioLegend), αGFP (1:5000 ...
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bioRxiv - Cancer Biology 2024Quote: Single cell suspensions from tumors were washed with HBSS containing 2% FBS and 1 mM EDTA and stained with the following antibody cocktails for detecting lymphoid populations: anti-CD45-PerCP/Cy5.5 (BioLegend 103132), anti-NK1.1-FITC (BioLegend 108706) ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with Brilliant Violet (BV) 421TM anti-human CD138 (Syndecan-1) antibody (BioLegend catalog #356516, clone MI15), FITC anti-human HLA-A ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Cancer Biology 2024Quote: ... The viability of T-ALL#1 cell suspension was assessed by flow cytometry with Zombie Aqua Viable dye (423102, BioLegend) prior to mouse injection ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 500 xg for 7 minutes and resuspended in 1 mL ice cold Cell Stain Buffer (CSB, BioLegend). Samples were filtered through a sterile pre-wet 70 μm mesh ...
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bioRxiv - Immunology 2024Quote: ... Cellular debris and red blood cells were removed by the Debris Removal Solution (Miltenyi) and 1× RBC Lysis Buffer (Biolegend), respectively ...
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bioRxiv - Immunology 2024Quote: ... and 0.75 ug of GAD and Empty tetramers in addition to 1:200 anti-human CD19-BV421 (BioLegend, Cat#363017) and 1:100 anti-human CD3-FITC (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Single-cell suspensions were generated as described above with an additional incubation with anti-CD11b-BV421 (1:200, Biolegend #101251) and anti-Ly6g-AF647 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... in the presence of anti-Ly6G AF488 (1A8) and anti-CD68 AF647 (FA-11) or their isotypes (1/100 each, Biolegend), and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then incubated 0.1 % BSA in PBS with or without 1/40 dilution of PE/Cyanine7-conjugated anti-human IL6 (Biolegend, #501119) or 1/80 dilution of PE-conjugated anti-human IL8 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in 100 µL of FACS Buffer and stained for 15 minutes at room temperature with an antibody cocktail containing anti-CD71 (1:50; PerCP/Cyanine 5.5; BioLegend, 334113), anti-CD235a (1:500 ...
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bioRxiv - Immunology 2024Quote: ... splenocyte and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: White blood cell and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 min at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were stained with Slices were stained with mouse anti-human Aβ1-16 antibody (6E10, Biolegend SIG-39320, 1:200) and detected using an IVIEW DAB Detection Kit (Ventana) ...
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bioRxiv - Immunology 2024Quote: Immune cells isolated above were stained with the following antibodies (Live/Dead Fixable NIR, Invitrogen, #L34975, 1:500; CD45, Pacific Blue, Biolegend, #103126 ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells from the mouse ear were sorted with a FACSAria sorter from samples treated with BHI-tween or HSV-1 and labeled with TotalSeqC hashtags antibodies (Biolegend). All samples were pooled together ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were washed with PBS and resuspended in 100 μL Zombie aqua cell viability dye solution (1:400 prepared in PBS, Cat. No. 423101, BioLegend) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were diluted 1:10 with 1% BSA ELISA assay diluent and ELISA was performed according to manufacturer’s instruction (ELISA MAX™ Standard Sets, BioLegend).
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the MDSC panel consisted of the following primary antibodies diluted in FACS buffer: anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell suspension was blocked by incubating with Fc Block and immunostained with CD11b-Brilliant Violet 785 (1:1000; Cat# 101243, BioLegend), CD206-APC (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with a mouse anti-LAMP1 conjugated with Alexa fluor 647 (BioLegend, clone H4A3, Cat #328609, 1:500) for 1h ...
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bioRxiv - Immunology 2024Quote: ... The remaining cells were then plated at 1×106/mL density in IMDM medium containing 20 ng/mL mouse M-CSF (BioLegend) and cultured for 7-9 days without medium change to generate BMDMs.
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bioRxiv - Immunology 2024Quote: Single cell suspensions were washed with PBS and resuspended in 100 μL Zombie aqua cell viability dye solution (1:400 prepared in PBS, Cat. No. 423101, BioLegend) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Then cells were incubated in the dark for 20 minutes with 50µL of the antibody mixture: 1:50 each of anti-CD3-AF488 (BioLegend 100210), anti-CD4-BV510 (BioLegend 100559) ...
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bioRxiv - Bioengineering 2024Quote: ... and vendor information of antibodies used for the flow panel in this study was as follows: (i) Live/Dead (BV510, 1:100, 423111; BioLegend), (ii ...
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bioRxiv - Systems Biology 2024Quote: ... after which the supernatant was discarded and 100µL of MojoSortTM Streptavidin Nanobeads (1:10 diluted in FACS buffer, Biolegend, 480016) was added to the mixture ...
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bioRxiv - Bioengineering 2024Quote: ... The following antibodies were used at a 1:100 dilution in Flow Cytometry Staining Buffer (eBiosciences #00-4222-26): CD45-PerCP Cy5.5 (Biolegend 103132), CD11b-Alexafluor 488 (thermofisher 53-0112-82) ...