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Citations for BioLegend :
8251 - 8300 of 8821 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... 500ul of fresh cell culture media for each respective cell type were combined with 10 µl of 0.5ug/µl purified anti-human ICAM-1 blocking antibody (Clone HA58, BioLegend), added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were blocked in 5% BSA in Tris-buffered saline/0.05% Tween-20 and proteins were detected by specific primary antibodies against MBP (#SMI 99, 1:1000; Biolegend), MOBP (bs-11184R ...
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bioRxiv - Neuroscience 2024Quote: ... isolated cells were stained with the following antibodies: CD11b-FITC (1:200 dilution, cat# 101205, clone M1/70, BioLegend), ACSA-2-PE (1:200 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... followed by an overnight incubation with the second set of primary antibodies [1/100 anti-CD9-biotin (312112, Biolegend) and anti-P2RY12)] ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were resuspended and blocked with 100 μL per well of 1:50 Mouse-Fc block (BioLegend #101320) in FACS buffer for 5 min ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies were added at the following concentrations: mouse anti a-syn antibody 1:750 (SIG-39730; BioLegend) and rabbit anti-LAMP1 1:2000 (ab278043 ...
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bioRxiv - Cancer Biology 2024Quote: ... and labeled with anti-MHC-I fluorescent antibody (anti-mouse MHCH-2kd/H-2Dd, BioLegend, Clone: 34-1-25). Cells were then washed with PBS and analyzed with the NovoCyte Advanteon Flow Cytometer (Agilent ...
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bioRxiv - Cell Biology 2024Quote: ... each well was coated with 70 µL of a 1:500 dilution of a biotinylated 6E10 antibody (#803008, BioLegend) in 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained with Pacific Blue™ (PB)-conjugated anti-CD45 antibody (#103126, BioLegend, CA, US; dilution 1:200), PB-conjugated anti-CD31 antibody (#102422 ...
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bioRxiv - Biochemistry 2024Quote: ... were pre-treated with the BLUE inhibitor panel (5 µM) or DMSO vehicle control for 1 hour followed by addition of recombinant IFNα2 (50 ng/mL, 592702, BioLegend). Following 1 hour incubation with IFNα2 ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to a 96-well plate and stained with zombie red (BioLegend, 1:1000 dilution in FACS wash buffer) for 30 min at room temperature in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... The expression of immune checkpoint receptors was determined using the following antibodies: PD-1 PE-Dazzle 594 (329940, Biolegend), VISTA BV421 (566750 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were filtered through 70μm and 40μm strainers and cells were stained with CD45-APC-Cy7 (Biolegend, 1:1000) and EpCAM-APC (Biolegend ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Unlabeled cells were then centrifuged at 300 x g and labeled with a cocktail of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer.
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Genetics 2021Quote: ... in 1 × phosphate-buffered saline with 0.1% Tween 20 (PBST) before being incubated with a 1:5000 dilution of anti-GAPDH-HRP (Biolegend, 649203) or 1:2000 dilution of anti-ACTIN-HRP (5125)
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to a 2 mL Eppendorf tube for immunostaining for 30 minutes on ice for the immune cells (CD45-PE/Cy7, 1:250, 103114, BioLegend), endothelial cells (ICAM2-A647 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in staining buffer (1% BGS-supplemented PBS) and incubated with anti-Flag-PE (clone L5; BioLegend #637310) and Ghost Dye Violet 510 (Tonbo Biosciences #13-0870-T100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
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bioRxiv - Immunology 2022Quote: ... CD3 localization was detected using FITC conjugated streptavidin (CALTAG Laboratories) and PSGL-1 localization was detected using anti-Rat IgG-PE (BioLegend) following a 30 minute incubation at 4°C ...
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bioRxiv - Immunology 2022Quote: ... CD8+ T cells were enriched via negative selection using biotinylated antibodies at the concentration indicated in Table 1 and using MojoSort Streptavidin Nanobeads (BioLegend). Enrichments were completed in 1X HBSS (Ca2+ and Mg2+ free ...
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bioRxiv - Immunology 2021Quote: Cells were stained with the following surface antibodies: Anti-human CD8a FITC (1:100, clone RPA-T8, BioLegend, cat # 301050), Anti-human TCRgd FITC (1:100 ...
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bioRxiv - Immunology 2021Quote: ... Nunc ELISA plates were coated with 1 μg/ml Spike S1+S2ECD-His recombinant protein (SinoBiologicals) in ELISA coating buffer (Biolegend) overnight at 4°C ...
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Immunology 2021Quote: ... 211-11A; Pepro Tech), IL-1β (1 ng/ml, 211-11B; Pepro Tech) or IFNα (100 ng/ml, 752802; Biolegend). Cytokines used in Figure 1 - figure supplement 1D were added at the concertation shown in Supplementary Table 1.
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BET protein inhibition regulates macrophage chromatin accessibility and microbiota-dependent colitisbioRxiv - Immunology 2021Quote: ... LPMCs and splenocytes were washed with cold 1x PBS and stained for viability followed by cell-surface marker staining for CD45 (1:100; Clone 30-F11, BioLegend), CD3ε (1:300 ...
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bioRxiv - Immunology 2021Quote: ... containing fluorescently labelled pMHC tetramers and/or fluorophore-conjugated antibodies (Table) at previously titrated working concentrations (usually 1:200 for commercially available antibodies from BioLegend). After 20 min ...
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bioRxiv - Cell Biology 2020Quote: ... LT-HSCs were stained with conjugated γH2AX antibody (FITC-conjugated anti-phospho-H2A.X antibody, Biolegend, clone 2F3, 1:50 dilution) for 2 hrs at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... Platelets were fixed with 1% formalin in phosphate buffered saline and incubated with phycoerythrin-labelled anti-CD62P (P-selectin; Biolegend) antibody following manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked in 10% BSA for 15 min and stained with 1:20 APC-anti-mouse IgD Ab (clone 11-26c.2a; 405713, BioLegend), FITC-anti-mouse IgM mAb (11-5790-81 ...
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bioRxiv - Immunology 2021Quote: ... Wells were cultured for 1 hour in a 10:1 ratio prior to being washed and stained with fluorochrome-conjugated Abs: anti-CD41 (clone MWReg- Biolegend) and anti-CD8 (clone 53.67- Biolegend) ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2021Quote: ... A maximum of 1×106 cells/donor were stained by oligo-barcoded antibodies (TotalSeq-A or TotalSeq-C; Biolegend, Expedeon) for 30 min on ice ...
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bioRxiv - Bioengineering 2020Quote: ... the single cell pellets were resuspended in 1mL FACS buffer containing 1:300 dilution of anti-EpCam-AF467 (Biolegend, 118211) (for lung and kidney epithelial cell ...
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bioRxiv - Bioengineering 2020Quote: ... Staining buffer used was a 1:1000 dilution of stock antibody solution (APC anti-mouse CD45; Alexa Fluor 488 anti-mouse Ly6G, Biolegend) into FACS buffer ...
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bioRxiv - Biochemistry 2021Quote: ... with remaining cells washed and resuspended in 1x PBS with 1% BSA and stained for 15 minutes with the following antibodies: human CD45-APC (Clone 2D1) (Biolegend), human CD33-PE-Cy7 (Clone P67.6 ...
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bioRxiv - Neuroscience 2021Quote: ... CD8+ T cells and CD45/CD11b+ cells (macrophages/microglia) was performed using the following antibodies in a 1:200 dilution: anti-CD3e (clone 145-2C11, BioLegend), anti-CD4 (clone GK 1.5 ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with 1.5% normal serum followed by incubation with the primary antibody (biotinylated anti-Aβ – 1:100, BioLegend, USA) for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were cleared by centrifugation and β1 integrin was immunoprecipitated with anti-β1 integrin (clone HMb1-1, BioLegend, #102202) and Dynabeads Protein G (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel Parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Cell Biology 2021Quote: ... Tissue sections were then washed and stained with an AF647-directly conjugated antibody towards CCR6 (1:200, clone G034E3, BioLegend) at 37 ° C for one hour ...
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bioRxiv - Immunology 2020Quote: A total of 1 x 106 mononuclear cells suspended in PBS were stained using fixable viability dye (Zombie NIR-Biolegend), and incubated for 15 minutes at room temperature following manufacturer’s recommendation ...