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Citations for BioLegend :
8501 - 8550 of 8821 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The following antibodies were used at a 1:100 dilution in Flow Cytometry Staining Buffer (eBiosciences #00-4222-26): CD45-PerCP Cy5.5 (Biolegend 103132), F4/80-FITC (Biolegend 157310) ...
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bioRxiv - Immunology 2024Quote: Aliquots of 5-10 x 106 cells were first assessed for viability staining using eFlour780 (eBioscience) fixable viability dye (1:1000) or 7AAD (Biolegend) viability dye (1:100) ...
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bioRxiv - Immunology 2024Quote: Cytokine levels were measured from culture media supernatants harvested 24 hours after plating using LegendPlexTM Human Inflammation Panel 1 (Biolegend), per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then washed twice with PBS before being incubated with Zombie NIR Fixable Viability dye (1:1000, BioLegend) for 15 min at RT ...
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bioRxiv - Physiology 2024Quote: ... The cell pellet was re-suspended in staining buffer supplemented with 1:100 anti-mouse CD16/32 (Fc block, BioLegend) and incubated on ice for 10 min ...
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bioRxiv - Bioengineering 2024Quote: Proapoptotic protein and cytokine release was measured in cell-free supernatants from 16h co-cultures of CAR-T and tumor cell lines at 1:1 E:T ratio and from 48h co-cultures of CAR-T and PDO at 10:1 E:T ratio using the Legendplex™ multi-plex bead-based immunoassay (cat. # 741187, Biolegend). For cytokine measurement ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining 80% cleared supernatant was transferred to a clean RNase-free tube and incubated with mouse anti-HA antibody (1:250; clone 16B12, Biolegend) on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked using 1% FBS diluted in permeabilization buffer for 15 mins and then stained with IFN-γ (Biolegend, clone: XMG1.2) and TNF-α (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: Lung cell suspensions prepared above were resuspended in 1 mL of PBS containing Zombie Green Viability Dye (0.1% Biolegend, 423112) and incubated at 4°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 250,000 cells were plated in 1 ml IMDM media with 20% FBS and 1% Penicillin Streptomycin containing 50 ng/ml recombinant mouse M-CSF (cat # 576406, Biolegend) in a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... hemolyzed lineage-positive and Sca-1-positive cells were labeled with biotin-conjugated antibodies and removed using MojoSort streptavidin nanobeads (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated on ice in the dark with APC anti-mouse Ly-6A/E (Sca-1) Antibody (clone D7, 108111, BioLegend) antibody for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... media was removed from cells and 45 µl of EMEM+1/100 Human TruStain FcX blocking solution (Fc Block, BioLegend) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were collected and washed three times with PBS and stained with 1:500 of FITC anti-γH2AX Phospho (Ser139) (BioLegend) antibody and Propidium iodide (1 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... were seeded in 48-well plate pre-coated with Purified anti-mouse CD3 (1:50 in PBS; clone 145-2c11; BioLegend) in RPMI supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... at a dilution of 1:5000 followed by Fc receptor blocking using a combination of 0.5 µL per test of Purified Anti-Mouse CD16/CD32 Fc block (Tonbo) and 1 µL per test of Human Trustain FcX (Biolegend). Samples and fluorescence minus one controls were stained using antibodies at the dilutions listed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). The PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...
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bioRxiv - Cancer Biology 2024Quote: ... 0.5 – 1 x 105 cells were washed with CSM and incubated with 100 µl antibody mix (1 µl APC anti-human CD19 (clone: HIB19, BioLegend); or 1 µl APC anti-human CD22 (clone ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed twice with CSM and incubated with 1 µl Pacific Blue anti-human CD19 antibody (clone: HIB19, BioLegend) in 100 µl CSM at RT for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellets were resuspended in 50 µL FACS buffer with antibodies targeting vimentin (VIM) (677804, Biolegend, dilution 1:400), Zinc finger E-box-binding homeobox 1 (ZEB1 ...
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bioRxiv - Cell Biology 2021Quote: ... Then 1×106 cells were washed again with 1x Hanks buffer and stained with 5µl of Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody (Biolegend#125608) in100µl BD Pharmingen™ Stain Buffer (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Staining of the red blood cell membrane was performed with the anti-Ter119::Alexa647 antibody (Biolegend, 0.5 mg/ml; 1/1000) for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone: 3G8) and 1 μg/ml APC anti-CD18 (clone: 1B4/CD18) (Biolegend). Cell data was collected using an Attune flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... ENR media was supplemented with 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of supernatants from cultures of T cell clones were collected after 48 hrs of stimulation and added to each well of 96-well round-bottom plates precoated with IFN-γ (clone MD-1) capturing antibodies (BioLegend). After overnight incubation ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked using PBS/1% BSA/0.5% Tween-20 and incubated with monoclonal mouse FITC anti BrdU (Biolegend, clone 3D4) at 1:50 dilution for 20 minutes ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in a solution of FACS buffer with a 1:100 dilution of PE anti-human B2-microglobin (Biolegend 316306). Cells were incubated for 30 mins in the dark at 4 °C ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and stained in 100 μl for 30 minutes on ice using an anti-mouse IgG-Brilliant Violet421 secondary antibody at 1:90 (BioLegend, 405317). Cells were again washed twice ...
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bioRxiv - Immunology 2021Quote: ... Samples were then washed twice with 1 mL of Intracellular Staining Perm Wash buffer (Cat: 421002, BioLegend, San Diego, CA, USA); after the second wash ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells are permeabilized with 0.1% Triton-X 100 for 10min at RT and stained with anti-vimentin (1:200, Biolegend, CA, USA) for 30 min in PBS containing 5% normal goat serum (RT) ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen-independent activation-marker expression of CAR-T cells was evaluated by antibody staining for CD137 (PE/Cy7, clone 4B4-1, BioLegend #309818) and CTLA-4 (PE/Cy7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was repeated on cultured CAFs and PVL cells using the previously described experimental conditions with anti-PDGFRα (1:100; BioLegend #323507), anti-CD146 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Systems Biology 2021Quote: Single nuclei from each PDX condition were labeled with 1 μl condition-specific hashtag oligonucleotide-labeled antibodies (BioLegend, TotalSeq A0541-A0545) according to manufacturer’s protocol prior to pooling and loading on a single lane of the 10X Chromium v2 system ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1-PE-Cy7 and FcγR-PE (eBioscience, 12-0161-83) for myeloid differentiation, or Mac-1-APC (eBioscience, 17-0112-82) and CD19-PB (BioLegend, 115523) for lymphoid differentiation on OP9 cells ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was removed and approximately 106 cells were resuspended in FACS buffer containing an anti-HA AF488 conjugate (1:1000, BioLegend, 901509) and an eFluor 780 fixable viability dye (Thermo Fischer Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in Cyto-Fast Wash Buffer and incubated in Cyto-Fast Wash Buffer containing an anti-HA AF647 conjugate (1:1000, BioLegend, 682404) and an anti-FLAG AF549 conjugate (for cells expressing FLAG-tagged M ...
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bioRxiv - Molecular Biology 2022Quote: ... Whole-lung suspension was then repelleted and resuspended in SB + 1:50 TruStain FcX™ antibody (anti-mouse CD16/32, BioLegend) for a 10-minute blocking period at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Antibodies used for removal of mouse stromal cells included: anti-mouse H-2Kd-biotin (BioLegend, 116604, clone SF1.1, dilution 1:20) and anti-mouse Cd45-PE/Cyanine5 (BioLegend 103110 ...
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bioRxiv - Immunology 2022Quote: ... 0.0032 μg SARS-CoV-1 biotinylated RBD protein (His & AVI Tag) (SinoBiological, 40634-V27H-B) conjugated with PE-streptavidin (BioLegend, 405204), 0.0032 μg SARS-CoV-2 biotinylated RBD protein (His & AVI Tag ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...