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Citations for BioLegend :
8701 - 8750 of 8821 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... 100 μL of PRP was incubated with 1 μg/mL AF647-conjugated CD42b rabbit anti-human antibody (BioLegend, San Diego, CA, USA) to label the platelets ...
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bioRxiv - Immunology 2022Quote: ... CD3ε clone 145-2C11; Ly-6G/Ly-6C clone RB6-8C5; CD11b clone M1/70; CD45R/B220 clone RA3-6B2; TER-119, Biolegend, 1/200). The c-kit− fraction was stained with antibodies against CD11b (PercPCy5.5 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed twice with PBS and stained using the Zombie NIR Fixable Viability Kit (Biolegend, 423106, diluted 1:500 in PBS) for 30 min at 4°C prior to Fc blocking and staining of surface antigens ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or TRIM5α-HA expression (using the CytoFix/Cytoperm kit [BD] according to manufacturer’s instructions and staining with anti-HA-PE [Biolegend] diluted 1:200). Cells were gated on size (FSC vs ...
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bioRxiv - Microbiology 2024Quote: Suspensions of ∼106 HIV-infected TMCs in PBS with 1% FBS were incubated with BV711 anti-human CD3 antibody (Biolegend, catalog # 300464) at 4°C for 30 min ...
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bioRxiv - Bioengineering 2024Quote: Supernatants were collected after a 48-hour PBMCs and TCM incubation and immediately assessed for cytokine secretion using LEGENDplex Human Inflammation Panel 1 (Biolegend catalog #740809) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... Fixation and cryostat sectioning of 3D organoids was performed as previously described.47 Immunostaining was done using antibodies to PAX6 (BioLegend, 1:200), SOX2 (Santa Cruz ...
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bioRxiv - Cancer Biology 2024Quote: ... for 2 minutes at room temperature and then cells were washed in PBS and incubated for 15 minutes at room temperature with a PBS solution containing the fixable viability dye Zombie VioletTM (Biolegend, 1:100) and CD16/CD32 Fc Block (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Zombie Nir™, Biolegend, Cat# 423105, dilution 1:400).
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bioRxiv - Immunology 2024Quote: ... 2×104 cells in 200 μL complete medium were stimulated with plate-coated OKT3 (as described above) and soluble 1 μg/mL anti-CD28 (BioLegend, clone CD28.2) for 24 hours ...
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bioRxiv - Immunology 2024Quote: ... Cryopreserved PBMC were thawed on ice and incubated with 100 μL of Zombie Live/Dead dye (diluted 1:400 in PBS) (Biolegend, London, UK) for every 5×106 cells at room temperature for 20 minutes ...
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bioRxiv - Immunology 2024Quote: ... T cells (both CD4+ and CD8+) within spleen cells were stimulated with 1 µg/ml of anti-CD3 (Biolegend, clone 145-2C11) and 5 µg/ml of anti-CD28 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... PBMC collected at baseline and 17 weeks after boosting were incubated for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Immunology 2024Quote: ... lymph node FNA samples collected 8 weeks post- boosting were incubated for 30 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Pathology 2024Quote: ... Sup-T1s were washed twice with FACS wash (PBS supplemented with 1% FCS; Capricorn Scientific) and subsequently stained with a cocktail of anti-human CD4-APC (BioLegend; clone OKT4) and anti-human HLA-A,B,C-BV605 (BioLegend ...
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bioRxiv - Pathology 2024Quote: ... BV785 CD45 (#103149) antibodies in blocking buffer at RT for 30 min (all from Biolegend and used at a 1:100 dilution). Cells were DAPI stained before sorting through BD FACSAria IIu Cell Sorter ...
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bioRxiv - Microbiology 2024Quote: ... buffer (PBS supplemented with 1% heat inactivated fetal bovine serum and 0.1% sodium azide) and blocked with TruStain FcX PLUS (BioLegend, San Diego, CA) for 15 min at 4°C ...
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bioRxiv - Bioengineering 2024Quote: Suspended cells prepared as above were stained for live/dead cell quantification with the Zombie NIR Fixable Viability Kit following with the manufacture’s protocol (1:100 dilution, 423111; BioLegend, San Diego, CA). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1:200 dilution of fluorochrome-conjugated antibodies for specific cell surface markers were used as follows: CD45 (Biolegend, clone: 30-F11), CD3ε (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once with flow buffer and incubated with TruStain FcX (anti-mouse CD16/32) Antibody (BioLegend, 101319; 1 μg/sample) in 50 μL volume for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed again with 1×PBS and then resuspended in 100 µl of PBS with Zombie NIR (for OT-I; Biolegend Cat#423105) and 7-AAD (for OT-II ...
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bioRxiv - Synthetic Biology 2024Quote: ... the cell pellet was resuspended in 100 µL of 1X Permeabilization Buffer and incubated with 1 µg of Alexa Fluor® 488 anti-human Ki-67 Antibody (BioLegend, 350532) for at least 30 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were washed 3x in FACS buffer than resuspended in 100 μL of FACS buffer with 1 μg of secondary antibody Goat anti-rat APC (Biolegend cat#405407) and incubated for 30 min in the dark on ice ...
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bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...
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bioRxiv - Neuroscience 2021Quote: ... The cell pellets were washed once with 15 mL PBS and resuspended in 1 mL of red blood cell (RBC) lysis buffer (BioLegend, San Diego, CA) for 2 min at room temperature to lyse RBCs ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed with FACs buffer (1X PBS, 1% FBS) and fixed at room temperature for 20 minutes with Intracellular Staining Fixation Buffer (BioLegend, San Diego, CA). Cells were washed with 1X Cyto-Fast™ Perm Wash solution (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... 20 μl cells were removed for flow analysis and remaining cells were incubated for 30 min on ice with 1 μg each of TotalSeq™-A0441 anti-mouse CD71 Antibody (Biolegend, Cat# 113824) and TotalSeq™-A0073 anti-mouse/human CD44 Antibody (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... Axons were identified using a staining against the phosphorylated neurofilaments (SMI31) stained with the PAB mouse-anti-SMI31 (Biolegend, cat. 801601, 1:250) and the SAB GAM-STAR580 (Abberior ...
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bioRxiv - Cell Biology 2021Quote: ... Immunostaining was performed with biotinylated monoclonal mouse antitubulin (clone 236-10501, Thermo, 1 μg/ml) then Brilliant Violet 421-conjugated streptavidin (Biolegend, 0.1 μg/ml), Alexa Fluor 488-conjugated mouse anti-perforin (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Splenocytes were then filtered through a 40μM filter and stained with an antibody cocktail [Pacific Blue anti-CD45.1 (Biolegend no. 110722; 1:100 dilution), APC anti-CD45.2 (Biolegend no ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Microbiology 2022Quote: ... single cell suspensions from mouse spleens were stained with fluorescein isothiocyanate (FITC)-conjugated anti-Ly-6A/E (Sca-1) mAb (Biolegend; San Diego, CA). To analyze erythrocyte maturation ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed in PBS/1% BSA buffer and incubated for 30 minutes on ice with the following antibodies from Biolegend (San Diego, USA): FITC-anti-human CD38 (#303503) ...
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bioRxiv - Neuroscience 2020Quote: ... and were stained at 20 μl of FACS buffer per 1×106 cells containing the first set of following antibodies (all from Biolegend, unless specified otherwise): biotinylated hematopoietic lineage antibodies against NK1.1 (clone PK136) ...
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bioRxiv - Immunology 2020Quote: A total of 1×106 splenocytes or liver lymphocytes were surface stained with Brilliant Violet (BV) 421-conjugated anti-CD107a (clone 1D4B, BioLegend, San Diego, CA) during the 24 h stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... and the nerve sections were incubated for 24 hours at room temperature with gentle rocking: mouse anti-TUJ1 for microtubules in the axons (1:1000, Biolegend Catalog No. 801202), rat anti-PECAM-1 for endothelial cells (1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: Single-cell suspensions were obtained as described above and subsequently blocked for 5-minutes on ice with Fc block CD16/32 (Biolegend, #101302, 1:100), followed by the addition of antibodies for 30-minutes on ice ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs for whole mount samples and cryosections were stained with a rabbit anti-keratin 14 (K14) polyclonal antibody (1:1000, 905301, BioLegend, San Diego, CA). MG whole mounts and cryosections were imaged with a Zeiss LSM880 confocal microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... freshly harvested PDX or EHS tumors were processed to single cells47 and stained with PE-conjugated anti-human CD298 PE (BioLegend; 1:50 dilution) and FITC-conjugated anti-mouse CD45 FITC (BioLegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated with Fc-blocking anti-CD16/32 anti-bodies followed by cell hashing antibody (1 μg/ml, BioLegend Cell Hashing A) (Stoeckius et al. ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed with FACS buffer followed by PBS wash and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) dye for 20 minutes at 4°C in dark ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed once in PBS and then fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) before staining with PU.1-PE (BioLegend 7C2C34 cat #681307) or rat IgG2a-PE isotype (BioLegend RTK2758 ...
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bioRxiv - Neuroscience 2023Quote: ... The following monoclonal antibodies were used for flow cytometry analyses at a dilution of 1/700: anti-CD45 (BioLegend, clone 30-F11, 103147), anti-CD11b (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... All cytokine antibodies were used at 1:100 dilution in 1x BD Perm/Wash buffer (anti-IFN-γ (APC/Cyanine7 or PE, Biolegend, #505849, #505808) and anti-TNF-ɑ (BV711 Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...