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Citations for BioLegend :
8601 - 8650 of 8821 citations for 1 6 ANHYDRO β D GLUCOSE 2 3 4 TRI O ACETATE since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cryopreserved PBMCs from blood collected 1 and 2 weeks after the H2-F boost were stained with anti-human monoclonal antibodies CD3 BV510 (OKT3, 1:400 dilution, BioLegend, RRID:AB_2561376), CD56 BV510 (HCD56 ...
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bioRxiv - Cancer Biology 2023Quote: ... FFPE tissue sections of PDX tumours and their equivalent primary tumour region were subjected to hematoxylin and eosin (H&E) staining or immunohistochemistry using the following antibodies: anti-human CD45 (Clone: HI30; 1:200; BioLegend, #304002), anti-keratin (Clone ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... cells were resuspended in FACS buffer and incubated with Alexa Fluor® 488 anti-CD31 ([390] 102414) and APC/Cyanine7 anti-CD45 ([30-F11] 103116) antibodies (1/100, BioLegend) and mouse TruStain FcXTM (1/200 ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used at a concentration of 1 µg/mL except where otherwise indicated: APC-Cy7-CD117 (clone 2B8; Biolegend #105826), PE-FceRI (clone MAR-1 ...
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bioRxiv - Immunology 2023Quote: Ligand-independent reporter cell activation was assessed by overnight stimulation with 6.25 µg mL-1 Ultra-LEAF™ Purified anti-human CD3 (OKT3, BioLegend, #317326). Cells were incubated in antibody-coated wells and TCR responses were measured by flow cytometric analysis of GFP expression after 16h ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants were at 8 h post-infection and used fresh or stored at -20°C for up to 1 week and TNF was quantified using mouse TNF-α ELISA MAX kit (BioLegend) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Tyrosine hydroxylase was visualized using an Alexa Fluor® 594 conjugated mouse monoclonal anti-TH antibody at a 1:500 concentration (818004; Biolegend). Glutamine synthetase was visualized using a mouse monoclonal anti-GS antibody at a 1:100 concentration (MA5-27750 ...
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bioRxiv - Immunology 2023Quote: The following list of antibodies and their concentration against mouse proteins were employed: αCD45 (1:400; BioLegend Cat# 103147, RRID:AB 2564383), αCD3ε (1:300 ...
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bioRxiv - Cancer Biology 2023Quote: Cells were stimulated with IFNγ for 4h and the trypizinized cells were immunostained with VCAM1 antibody (1:100 dilution, BV421; BioLegend #305815). eBioscience™ Fixable Viability Dye eFluor™ 780 was used for discriminating dead cells ...
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bioRxiv - Bioengineering 2023Quote: ... Cells pre-treated with anti-mouse TfR-1 antibodies (human constant regions) were incubated with 50 nM of a second TfR-1 antibody for detection (R17, rat anti-mouse antibody; BioLegend, 113802). Cells pre-treated with anti-mouse CD98hc antibodies (human constant regions ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions of samples incubated with myeloid antibody cocktail were washed and resuspended in PBS for flow cytometry run whereas lymphoid antibody cocktail-stained cells were incubated overnight with 1 mL perm buffer (Biolegend; 421402) containing 10 μL of anti-FoxP3 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were then incubated in 1×PBST with either rabbit anti-flagellin (kindly given by Dr Monica Rolando) or mouse anti-RNAPα (BioLegend #663104) diluted 1/5,000 for 1h at room temperature with agitation ...
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bioRxiv - Cell Biology 2023Quote: ... cell suspensions were incubated for 20 min on ice with TotalSeq™-C anti-human Hashtag oligos (HTOs) (1:500, Biolegend, 394661 ...
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bioRxiv - Cancer Biology 2023Quote: ... Phenotyping of AML patient samples used a 1:50 dilution of the PE-Cy7-conjugated anti-human CD34 antibody (clone 581, Biolegend, UK). Samples were stained ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 million cells per condition were then stained with phycoerythrin-(PE)-conjugated anti-human CD147 antibody diluted 1:1000 in flow buffer (BioLegend #306211) for 20 min on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed in 1X PBS and stained with Alexa Fluor 700 anti-human B2M antibody (BioLegend, 395708; 1:20 dilution) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then stained with standard antibodies for immune and vascular lineage exclusion: anti-CD45-PE/Cy7 (BioLegend, 304016, 1:200), anti-CD31-PE/Cy7 (BioLegend ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: The resultant single cells were stained with cocktails of antibodies diluted with FACS buffer listed as follows: PE-conjugated anti-mouse/human CD324 (E-Cadherin) antibody (1:500, Biolegend, 147303), PE/Cy7-conjugated anti-mouse CD117 (c-Kit ...
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bioRxiv - Immunology 2023Quote: ... Cells were then stained with the following antibodies on ice for 30 minutes: Alexa Fluor 647 anti-human CD3 (1:100 dilution, BioLegend, 300321), Alexa Fluor 488 anti-human CD4 (1:200 dilution ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Maxpar Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) for FC blocking (10 min ...
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Treg cells drive MYCN-mediated immunosuppression and tumor aggressiveness in high-risk neuroblastomabioRxiv - Cancer Biology 2023Quote: ... cells from both upper and lower chambers were collected and stained with BV421-conjugated CD4 (1:100, Clone RPA-TA, BioLegend, 300501) and APC/CY7-conjugated CD8 (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... in phenol-red free DMEM-F12 in combination with live-dead stain Zombie NIR (1: 10,000 dilution; Biolegend, cat. no 423106) and incubated for 25 minutes at 37 °C and 5% CO2 conditions ...
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bioRxiv - Biochemistry 2023Quote: ... FoxP3-transduced CD4+ T cells were stained with antibodies targeting the cell surface antigens CD25 (Biolegend, Cat#102022, 1:1000 dilution) and Thy1.1 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Following incubation on ice for 30 min., cells were pelleted, washed, and resuspended in PBS/BSA containing streptavidin-APC (1:2000 dilution in PBS/BSA, (BioLegend #405207)) and incubated for 30 min on ice prior to washing ...
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bioRxiv - Immunology 2023Quote: ... 6 x 106 cells were stained with the appropriate cocktail of cell-surface conjugated antibodies in 200µL FWB with the fixable viability dye Zombie Red (BioLegend; 1:1000) for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2023Quote: ... About 1*106 live cells were then blocked with TruStain FcXTM anti-mouse CD16/32 antibody (Clone 93; Biolegend; Cat #101320) on ice for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... and incubated in 100 μl of Cell Staining Buffer with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:100; 101320, Biolegend) and True-Stain Monocyte Blocker™ (1:20 ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected on day 6 and immediately assessed for cytokine and chemokine secretion by LEGENDplex™ using LEGENDplex Human Inflammation Panel 1 (13-plex) with V-bottom Plate (Biolegend catalog #740809 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained with the following conjugated cell-surface marker antibodies at 1:50 dilutions: CD326 (EpCAM) PE (Biolegend, 324206) and CD45 BV786 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were permeabilized for intra-cellular staining with a saponin-based buffer (1% saponin, 10% BSA in PBS) and stained with an antibody for Cytochrome C AlexaFluor 647 (Biolegend, 612310) used at 1:2000 dilution and DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were washed with DPBS three times and stained with 1 µg/mL Streptavidin-Alexa Fluor 647 (AF647) (BioLegend, #405237) for another 30 min ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Cell Biology 2024Quote: ... The live mCherry+ or mCherry- Epcam+ miPSC or GFP+ hiPSC were sorted using the following staining: anti-Epcam antibody (1:50) (Biolegend; 118225). Cells were loaded on a 10x Genomics Chromium controller.
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated for 25 m on ice in a cocktail of fluorochrome-conjugated antibodies (B220 (RA3-6B2-BV605; 1:200; BioLegend #103244), TCRβ (H57-597-PE-Cy7 ...
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bioRxiv - Developmental Biology 2024Quote: ... or with an anti-integrin ß4 antibody coupled to R-phycoerythrin (ITGß4-PE, 1:500, clone 58XB4, BioLegend, San Diego CA) for isolation of CTB ...
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bioRxiv - Microbiology 2024Quote: ... each sample was counterstained with the desired concentration (0.5ul/1 × 106 cells) of 7AAD viability staining solution (Biolegend; Cat. No. 420404) before analysis ...
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bioRxiv - Immunology 2024Quote: ... cell pellets resuspended in 100 μl FACS buffer (phosphate buffer saline containing 1% BSA and 0.1% sodium azide) were treated with human TruStain FcX™ (Biolegend #422302) for 15 min on ice followed by incubation with rabbit anti-human LBR antibody (Abcam #32535 ...
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bioRxiv - Immunology 2024Quote: 2×106 cells per sample were incubated at room temperature with 50 μL of TruStain FcX (αCD16/CD32, 1:100, Biolegend #101319) for 20 minutes before staining with the Zombie UV Fixable Viability Stain (1:1000 ...
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Langerhans cells regulate immunity in adulthood by regulating postnatal dermal lymphatic developmentbioRxiv - Immunology 2024Quote: ... The media was changed with new serum free media and incubated with his tagged recombinant mouse VEGF-C (10 μg/mL) for 30 min before staining in PBS with anti-his PE (1:100, BioLegend, #362603) and cell surface markers.
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bioRxiv - Neuroscience 2024Quote: ... and the cell pellet was resuspended at the density of 1 × 106 cells/ml in 100 µl Annexin V binding buffer (BioLegend, #422201). All the following steps were performed on ice ...
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bioRxiv - Synthetic Biology 2024Quote: ... washed once with 1X PBS and incubated with Zombie NIR viability dye (1:750, Biolegend, San Diego, CA, USA; cat#423105) in PBS for 20 min ...
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bioRxiv - Systems Biology 2024Quote: ... cells were passaged on geltrex-coated wells with the 1 μL/cm2 and were repeatedly passaged by dissociation with Accutase (Biolegend, 423201) every 3-5 days for five successive passages.
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bioRxiv - Immunology 2024Quote: ... Cells were stained with Zombie NIR viability dye for 10-15 minutes at room temperature (Biolegend, Cat. 423106, 1:1000 dilution), followed by quenching and washing with FACS buffer ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Cell Biology 2024Quote: ... Cell pellets were gently resuspended in 100 µl FACS buffer and stained with antibodies (CD38-APC, Clone: HIT2 (Biolegend, 1:100), CD45RA-PE-Cy7 ...
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bioRxiv - Cancer Biology 2024Quote: ... single cells isolated from tissue were labeled for 30 min at room temperature with Alexa Fluor 647-conjugated anti-EpCAM (1:50, Biolegend 324212), PE-conjugated anti-CD49f (1:100 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Anti-mouse primary antibodies were used at the following dilutions to define immune cell populations (Fig. S3): Ly6G (1:100; Biolegend, 127628), CD45 (1:100 ...
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bioRxiv - Neuroscience 2024Quote: A combination of the following primary antibodies was used for immunostaining of whole-mount meninges: anti-Ly6G (1:200 BioLegend 127626), anti-CD11b (1:200 BioLegend 101220 ...