Labshake search
Citations for BioLegend :
7451 - 7500 of 8186 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2023Quote: ... for 30 minutes at 37°C and then incubated with 10 µg/ml of αASC TMS-1 (clone HASC-71, Biolegend) in blocking buffer ...
-
bioRxiv - Immunology 2022Quote: T cells were adjusted at a concentration of 1 × 106 cells/100 μl CTL medium including Golgi-Stop (Biolegend, 420701) and Golgi-Plug (Biolegend ...
-
bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
-
bioRxiv - Microbiology 2023Quote: ... percoll isolated cells were activated for 5 hours at 37 °C using a Cell Activation Cocktail (1:500, Biolegend, USA). The cells were washed with PBS once and stained with surface staining antibody for 40min at 4 °C ...
-
bioRxiv - Developmental Biology 2023Quote: ... An aliquot of the resulting suspensions in PBS were immediately stained for viability with Zombie Aqua diluted 1:1000 (BioLegend) for 20 min at 4°C ...
-
bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
-
bioRxiv - Microbiology 2023Quote: ... Adjacent sections were deparaffinized in citrate and stained with monoclonal mouse anti-F4/80 antibody at a 1:400 dilution (BioLegend), or polyclonal goat anti-MPO antibody at a 1:500 dilution (R&D Systems) ...
-
bioRxiv - Cancer Biology 2023Quote: ... Wells of a 24-well plate were coated with 1 μg/mL anti-CD3 (#317326) and anti-CD28 antibodies (#302934, both BioLegend). For activation of T-cells ...
-
bioRxiv - Immunology 2023Quote: ... the slides were incubated for 1 hour in the dark at room temperature with an Alexa 488-conjugated goat anti-mouse IgG antibody (BioLegend). Following three washing steps ...
-
bioRxiv - Immunology 2023Quote: ... stained with a mixture of fluorochrome-conjugated antibodies (1:200 FITC anti-mouse/human CD11b M1/70, BioLegend, California, USA), (1:200 APC/Cyanine7 anti-mouse F4/80 ...
-
bioRxiv - Developmental Biology 2023Quote: ... When staining for SLAM markers the same lineage cocktail was used (FITC) with the addition of the following antibodies: anti-CD48 (FITC, clone HM48-1, BioLegend), anti-CD41 (FITC ...
-
bioRxiv - Immunology 2023Quote: ... Pacific Blue (or v450), and APC-Cy7 (or Alexa Fluor-e780)-conjugated antibodies (1:100, CD4, CD8) purchased from Biolegend. After cell surface marker staining ...
-
bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45lowSiglec-H+ microglia were labeled with rat anti-ICAM1 BV711 (1:100, catalog no. 116143; BioLegend), rat anti- Ly6A/E FITC (1:100 ...
-
bioRxiv - Immunology 2023Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
-
bioRxiv - Immunology 2023Quote: ... Cells were then permeabilized using BD Fix/Perm for 20 min at RT and stained intracellularly with anti-IFN-γ (1:100 dilution; clone XMG1.2, Biolegend), anti-TNF-α (1:100 dilution ...
-
bioRxiv - Microbiology 2023Quote: ... for 1 hour at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
-
bioRxiv - Immunology 2023Quote: ... washed FACS buffer and stained with a 1:100 dilution of anti-mF4/80 or anti-m/hCD11b antibody (Biolegend) FACS buffer for 30 min at 4 °C ...
-
bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were restimulated at 1×106 cells/mL concentration for 48 h on day 8-10 with 1 µg/mL plate-bound anti-CD3ε antibody (clone UCHT1, Biolegend). BiTE-mediated killing assays were carried out on day 14-16 of culture.
-
bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
-
bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
-
bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
-
bioRxiv - Cell Biology 2024Quote: ... Cells from the epithelial layer were sorted gating on single positive for CD326-PE/Cy7 (BioLegend, clone DECMA-1, #147310) and CD104-AF647 (BioLegend ...
-
bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
-
bioRxiv - Genomics 2024Quote: ... amplification and sequencing libraries were generated using the Next GEM Single Cell 3’ Kit v3.1 (10X Genomics) with the recommended modifications for compatibility with the TotalSeq-A cell hashing and CITE-seq reagents (BioLegend). Organ donor GEX libraries were sequenced on a NovaSeq 6000 (Illumina ...
-
bioRxiv - Cancer Biology 2024Quote: ... Mouse lung single cells were similarly stained but with a cocktail of antibodies (1:250 each) against CD45-PE/Cy7 (103114, BioLegend), Icam2-A647 (A15452 ...
-
bioRxiv - Neuroscience 2024Quote: ... FluoSphere- containing media was then removed from the wells and cells were processed for flow cytometry using markers for CD11b (1:200, 101206, Biolegend) and CD45 (1:200,103132 ...
-
bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
-
bioRxiv - Immunology 2023Quote: ... samples from animals that were vaccinated with the recombinant GPCysR4-I53-50A.30 To exclude I53-50A-binding B cells we included a SARS-CoV-1 Spike probe containing I53-50A-biotin which was labeled with streptavidin AF647 and streptavidin BV421 (both Biolegend). After a 2 day culture with interleukin (IL)-21 and irradiated mouse fibroblast (L cells ...
-
bioRxiv - Neuroscience 2024Quote: ... and heavy non-phosphorylated neurofilament (NF-H) enriched in large pyramidal neurons (Anti-NF-H mouse monoclonal antibody, clone SMI32, 1:2000, Biolegend). All sections were counterstained with hematoxylin for further visualization of cell organization used in cytoarchitectonic type identification explained below and in Supplementary Table 1.
-
bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
-
bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBST and streptavidin-conjugated to horseradish peroxidase was added (1:2000 in PBS, BioLegend) for 30 minutes at RT ...
-
bioRxiv - Immunology 2024Quote: ... anti-SHIP-1 (P1C1-A5, PE), human IgE (MHE-18, Alexa Fluor 647), HLA-DR (L243, APC-Cy7) (all from Biolegend); and phospho-p65 NF-κB (K10-895.12.50 ...
-
bioRxiv - Immunology 2024Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
-
bioRxiv - Cell Biology 2024Quote: ... collected in PBS + 1% FBS and stained for ICAM-1 expression on the endothelial cell surface using APC anti-human CD54 antibody (353111, Biolegend). Flow cytometry analysis was performed and confirmed that ICAM-1 expression was upregulated following overnight stimulation with TNF in each of the co-culture experiments.
-
bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: APC anti-mouse I-A/I-E (MHC II) (clone M5/114.15.2, BioLegend, 1:500), FITC anti-mouse I-A/I-E (MHC II ...
-
bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: Alexa Fluor 488 anti-mouse CD3 (17A2) (1:100) (BioLegend, San Diego, CA), PE anti-mouse CD4 (GK1.5 ...
-
bioRxiv - Immunology 2024Quote: γδ IELs were cultured ex vivo as previously described (33) in which 100,000 γδ IELs were activated with 1 ÿg/mL anti-CD3 (2C11, BioLegend) in the presence of 10 U/mL IL-2 ...
-
bioRxiv - Immunology 2024Quote: ... and anti-dsDNA antibodies were detected with a 1/1000 dilution of alkaline phosphatase–conjugated goat anti-mouse IgG (BioLegend) for 1 hour at 37°C and developed with a phosphatase substrate for 30 min at 37°C.
-
bioRxiv - Immunology 2024Quote: ... and stained with mouse anti-human HLA-E primary antibody or mouse anti-human HLA class I primary antibody (clone W6/32, purified from hybridoma cells) in 1:100 dilution with cell staining buffer (BioLegend) and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend) ...
-
bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
-
bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
-
bioRxiv - Immunology 2023Quote: ... 200 μl for 96-well plates) supplemented with 1 μg/ml of recombinant human B cell-activating factor (BAFF, BioLegend). 1 µg/ml rHA from H1N1 (PR8 ...
-
bioRxiv - Immunology 2024Quote: ... Cells were then washed and stained for 30min at 4°C in the dark with Fc block diluted 1:200 and subsets of the following antibodies: anti-CD103 (2E7, Biolegend), anti-CD11b (M1/70 ...
-
bioRxiv - Cell Biology 2023Quote: ... Neurons were then maintained for 10 min at 37°C in the presence of AlexaFluor-647-conjugated AnxA5 (1/50, Biolegend) in resting solution (Locke’s solution:140 mM NaCl ...
-
bioRxiv - Molecular Biology 2023Quote: Cells were incubated for 30 minutes at room temperature in 0.5% BSA PBS with 1:50 CD81-FITC antibody (Biolegend, 349504) or mouse IgG1 FITC isotype control antibody (Biolegend ...
-
bioRxiv - Physiology 2023Quote: ... Serum samples were prepared for Legendplex assays by diluting 1:50000 for the detection of immunoglobulin isotypes using the mouse immunoglobulin isotyping kit (BioLegend). The assay was performed in a V-bottom plate and data was acquired on a BD Fortessa X-30 cytometer (BD Biosciences) ...
-
bioRxiv - Microbiology 2023Quote: ... Dead cells were stained using 40 µl/well of a cocktail of Annexin V – FITC (#640906; Biolegend, 1:50 dilution) and 7 AAD (#420404 ...
-
bioRxiv - Immunology 2023Quote: ... B cells were gated for using 1:20 dilution commercial mouse anti-human CD19-Alexa488 (BioLegend, San Diego, CA, USA) and 1:20 dilution mouse anti-human CD27-PE (stock 50 ug/mL ...