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Citations for BioLegend :
7601 - 7650 of 8186 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The cell pellets were resuspended in 50 µL FACS buffer with antibodies targeting vimentin (VIM) (677804, Biolegend, dilution 1:400), Zinc finger E-box-binding homeobox 1 (ZEB1 ...
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bioRxiv - Cancer Biology 2024Quote: ... at a dilution of 1:5000 followed by Fc receptor blocking using a combination of 0.5 µL per test of Purified Anti-Mouse CD16/CD32 Fc block (Tonbo) and 1 µL per test of Human Trustain FcX (Biolegend). Samples and fluorescence minus one controls were stained using antibodies at the dilutions listed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). The PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 stimulation group (LN stim) with hashtag 4 (BioLegend, Cat# 155807); lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend) monoclonal anti-CD10 ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were cultured for 4 hours in the presence of PMA and Ionomycin (Cell Stimulation Cocktail, Biolegend) and Brefeldin A (Biolegend) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse CD8a (Biolegend, PE/Cy5), CD4 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... stained for 30 minutes at 4℃ with the following antibody: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend), PE/Cyanine7 anti-mouse CD4 antibody (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...
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bioRxiv - Immunology 2020Quote: The isolated cells were then stained for 30 min at 4°C with the following antibodies (Biolegend): APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Cell Biology 2021Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...
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bioRxiv - Immunology 2022Quote: ... Samples were incubated overnight at 4°C with anti-CD15(HI90)-Bi209 (primary antibody purchased from Biolegend; conjugated with Fluidigm’s MaxPar labeling kit ...
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bioRxiv - Immunology 2024Quote: ... Staining with primary antibodies was performed overnight at 4°C in blocking buffer (LN: IgD (Biolegend, 405705), and CD8 (Abcam ...
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bioRxiv - Immunology 2024Quote: ... Cells were fixed for 5 to 10 min at room temperature in 4% paraformaldehyde fixation buffer (BioLegend). Cells were permeabilized for 30 min at 4°C in PhosFlow Perm Buffer III (BD Biosciences) ...
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bioRxiv - Immunology 2023Quote: ... for 10min at 4°C, then FcR (Miltenyi, Cat. 130-092-575) and monocyte (Biolegend, Cat. 2000135006) blocking for 10min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... at 4°C) and stained with a fixable viability dye (Zombie NIR™ Fixable Viability Kit, Biolegend) in PBS for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11 ...
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bioRxiv - Immunology 2023Quote: ... 4°C) the cells were stained with fluorochrome-conjugated antibodies for FACS Analysis (all antibodies from Biolegend) for 15 min ...
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bioRxiv - Bioengineering 2023Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse GL7 (Biolegend, Pacific Blue), IgM (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained for 5 min at 4°C with biotinylated anti-CD19 antibody (1D3; BioLegend) to exclude CD19-positive B cells in the cell suspension using anti-biotin microbeads and LS columns ...
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bioRxiv - Immunology 2024Quote: ... ELISA plates were coated with purified antibody (3 µg/ml) overnight at 4°C (BioLegend, catalog 519202). The next day ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes at 4 °C and then stained with FITC - anti-human EPCAM (BioLegend CAT #32404) and PE - anti-CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... and permeabilized for 20 min at 4℃ with intracellular staining permeabilization wash buffer and fixation buffer (Biolegend). Next ...
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bioRxiv - Cell Biology 2024Quote: Cells were stained at 4°C for 20 min with lineage markers including CD45-BV421 (Biolegend, 103134), Ter119-BV421 (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Then 1×106 cells were washed again with 1x Hanks buffer and stained with 5µl of Alexa Fluor® 647 anti-mouse/human CD15 (SSEA-1) Antibody (Biolegend#125608) in100µl BD Pharmingen™ Stain Buffer (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... Staining of the red blood cell membrane was performed with the anti-Ter119::Alexa647 antibody (Biolegend, 0.5 mg/ml; 1/1000) for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... and cells were stained with 1.5 μg/ml Brilliant Violet 605™ anti-CD16 (clone: 3G8) and 1 μg/ml APC anti-CD18 (clone: 1B4/CD18) (Biolegend). Cell data was collected using an Attune flow cytometer (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2021Quote: ... ENR media was supplemented with 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were pelleted by centrifugation and then resuspended in 100 μL FACs buffer (PBS with 10 % FCS) with PE-conjugated anti-CD61 (RRID:AB_313084, Biolegend, 104307, 1:200) and Alexa Fluor 647-conjugated anti-SSEA1 (RRID:AB_1210551 ...
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bioRxiv - Immunology 2022Quote: ... 50 μl of supernatants from cultures of T cell clones were collected after 48 hrs of stimulation and added to each well of 96-well round-bottom plates precoated with IFN-γ (clone MD-1) capturing antibodies (BioLegend). After overnight incubation ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were blocked using PBS/1% BSA/0.5% Tween-20 and incubated with monoclonal mouse FITC anti BrdU (Biolegend, clone 3D4) at 1:50 dilution for 20 minutes ...
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bioRxiv - Genomics 2020Quote: ... and resuspended in a solution of FACS buffer with a 1:100 dilution of PE anti-human B2-microglobin (Biolegend 316306). Cells were incubated for 30 mins in the dark at 4 °C ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3–8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... This was followed by an incubation period with Anti-Aβ mouse monoclonal antibody 6E10 (1:1000, epitope: Aβ3-8, Biolegend, San Diego) and peroxidase-conjugated anti-mouse IgG (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice and stained in 100 μl for 30 minutes on ice using an anti-mouse IgG-Brilliant Violet421 secondary antibody at 1:90 (BioLegend, 405317). Cells were again washed twice ...
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bioRxiv - Immunology 2021Quote: ... Samples were then washed twice with 1 mL of Intracellular Staining Perm Wash buffer (Cat: 421002, BioLegend, San Diego, CA, USA); after the second wash ...
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bioRxiv - Cell Biology 2021Quote: ... The fixed cells are permeabilized with 0.1% Triton-X 100 for 10min at RT and stained with anti-vimentin (1:200, Biolegend, CA, USA) for 30 min in PBS containing 5% normal goat serum (RT) ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Bioengineering 2020Quote: ... Antigen-independent activation-marker expression of CAR-T cells was evaluated by antibody staining for CD137 (PE/Cy7, clone 4B4-1, BioLegend #309818) and CTLA-4 (PE/Cy7 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell sorting was repeated on cultured CAFs and PVL cells using the previously described experimental conditions with anti-PDGFRα (1:100; BioLegend #323507), anti-CD146 (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-DnaK 4RA2 at 1□:□10,□000 dilution (Enzo, ADI-SPA-880) and HRP Goat anti-mouse IgG (BioLegend, 405306) at 1□:□10,□000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... PBMCs were collected and stained using APC/Fire anti-CD4 and APC anti-CD8 (Supplemental Table 1) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supplemental Table 1 ...
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bioRxiv - Cell Biology 2021Quote: ... CD59 (PE, 143103), PD-1 (BV605, 135220), CD44 (BV711, 103057), CD69 (PE-Cy7, 104512), IFN-γ (APC, 505810) (all from Biolegend). The Foxp3 Fix/Perm Kit was used for intracellular staining (421403 ...
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bioRxiv - Microbiology 2021Quote: We performed western blotting as in (Polino et al., 2020) with primary antibodies mouse anti-HA diluted 1:1000 (clone 16B12; Biolegend #901501), rabbit anti-PfAldolase diluted 1:2000 (abcam #ab207494 ...
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bioRxiv - Systems Biology 2021Quote: Single nuclei from each PDX condition were labeled with 1 μl condition-specific hashtag oligonucleotide-labeled antibodies (BioLegend, TotalSeq A0541-A0545) according to manufacturer’s protocol prior to pooling and loading on a single lane of the 10X Chromium v2 system ...