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Citations for BioLegend :
7301 - 7350 of 8186 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... followed by an overnight incubation with the second set of primary antibodies [1/100 anti-CD9-biotin (312112, Biolegend) and anti-P2RY12)] ...
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bioRxiv - Immunology 2024Quote: ... brefeldin A (1 μg/mL; eBioscience, Cat. No. 00-4506-51) and monensin (0.5 μM; Biolegend, Cat. No. 420701) were added as well ...
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bioRxiv - Immunology 2024Quote: ... + 5 ml FBS + 2mM Ethylenediaminetetraacetic acid [EDTA]) with Human TruStain FcX™ Fc Receptor Blocking Solution (1:20; BioLegend) for 15 minutes at room temperature in foil ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with a cocktail of antibodies and the Zombie NIR fixable viability kit (1:1000, Biolegend, 423105) prepared in PBS for 20 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were resuspended in cell staining buffer (PBS + 5 mM EDTA + 1% BSA + 1.6 mM NaOH + 0.01% sodium azide) and incubated in 1:100 diluted Zombie UV viability dye (Biolegend, 423107) or Zombie Violet viability dye (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: The Chemokine concentrations in cell culture media were assessed using the 13-plex LEGENDplex Human Inflammation Panel 1 (Biolegend), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed three times in PBS and stained with 1:50 anti-human IgG APC (Biolegend, cat# 409306) and 1:2000 SYBR green (Invitrogen cat # S7563 ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were resuspended and blocked with 100 μL per well of 1:50 Mouse-Fc block (BioLegend #101320) in FACS buffer for 5 min ...
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bioRxiv - Immunology 2024Quote: ... and individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
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bioRxiv - Immunology 2024Quote: ... individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...
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bioRxiv - Immunology 2024Quote: ... live cells were counted and cultured in complete medium with anti-CD3 (1 μg/ml, clone 30-F11; Biolegend) and anti-CD28 (1 μg/ml ...
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bioRxiv - Immunology 2024Quote: ... with the addition of either DCs (1 cell:3 Tresp) with 0.5 μg/ml purified anti-CD3 (145-2C11, Biolegend), or Dynabeads Mouse T-Activator CD3/CD28 for T-Cell Expansion and Activation (Gibco ...
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bioRxiv - Neuroscience 2024Quote: ... The primary antibodies were added at the following concentrations: mouse anti a-syn antibody 1:750 (SIG-39730; BioLegend) and rabbit anti-LAMP1 1:2000 (ab278043 ...
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bioRxiv - Cancer Biology 2024Quote: ... and labeled with anti-MHC-I fluorescent antibody (anti-mouse MHCH-2kd/H-2Dd, BioLegend, Clone: 34-1-25). Cells were then washed with PBS and analyzed with the NovoCyte Advanteon Flow Cytometer (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stained with Pacific Blue™ (PB)-conjugated anti-CD45 antibody (#103126, BioLegend, CA, US; dilution 1:200), PB-conjugated anti-CD31 antibody (#102422 ...
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bioRxiv - Cell Biology 2024Quote: ... each well was coated with 70 µL of a 1:500 dilution of a biotinylated 6E10 antibody (#803008, BioLegend) in 0.05% Tween-20 ...
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bioRxiv - Cancer Biology 2024Quote: ... The expression of immune checkpoint receptors was determined using the following antibodies: PD-1 PE-Dazzle 594 (329940, Biolegend), VISTA BV421 (566750 ...
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bioRxiv - Cancer Biology 2024Quote: ... transferred to a 96-well plate and stained with zombie red (BioLegend, 1:1000 dilution in FACS wash buffer) for 30 min at room temperature in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... were pre-treated with the BLUE inhibitor panel (5 µM) or DMSO vehicle control for 1 hour followed by addition of recombinant IFNα2 (50 ng/mL, 592702, BioLegend). Following 1 hour incubation with IFNα2 ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were filtered through 70μm and 40μm strainers and cells were stained with CD45-APC-Cy7 (Biolegend, 1:1000) and EpCAM-APC (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated overnight at 4 °C with the primary antibodies against ASC (HASC-71, BioLegend). Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20 ...
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bioRxiv - Immunology 2021Quote: ... After 4 hours BMDMs were harvested and stained with an antibody against F4/80 (BM8, Biolegend) and propidium iodide (BD Biosciences) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse B220 (Biolegend, APC), GL7 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were incubated for 15 minutes at 4°C in streptavidin-PE/Cy7 (BioLegend) to detect NCAM-biotin ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were first incubated for 1h at 4°C with 2μl of anti- HA antibody (BioLegend) and then for another hr after adding 30μl of washed magnetic ProteinG beads (Cytiva) ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Cells were then incubated for 10 minutes at 4°C with True stain Fc block (BioLegend) and washed ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight staining at 4°C with rat anti-mouse CD45 primary antibody (103102, Biolegend) diluted 1:500 in 1x D-PBS ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend) were added to the neutralized FA fraction ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was removed by aspiration and 500 microliters of fixation buffer (4% paraformaldehyde in PBS, Biolegend) added to each well and the slide kept in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and stained (30 min at 4°C) with PE anti-TLR4 (Biolegend; clone Sa15-21; 145404) in blocking buffer (PBS with 2% rat serum) ...
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bioRxiv - Immunology 2024Quote: ... 10 µg/ml of Ultra-LEAFTM purified anti-mouse IL-4 antibody (clone 11B11, Biolegend, #504122), 10 ng/ml animal-free recombinant murine IFNγ (PeproTech ...
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bioRxiv - Microbiology 2024Quote: ... at 10 ng/mL (growth media) and 4 µg/mL of anti-CD28 antibody (Biolegend, #302934). Cells were plated in non-TC treated 24-well plates previously coated with 2 µg/mL of anti-CD3 antibody (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... cells were stained for 30 min at 4°C with antibodies (from BioLegend or BD Biosciences) against CD3 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The knock-out of the T cell receptor (TCR) was assessed with the absence of signal after staining 1:200 with APC-labeled anti-CD3ε (Biolegend). Each CAR construct contained a Strep tag which allowed for a two-step staining to validate successful knock-in ...
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bioRxiv - Cell Biology 2020Quote: ... Primary NK cells from lineage-depleted apheresis or NK92 cells were incubated on cells or cell-free matrix for 30 minutes in the presence of directly conjugated antibodies to surface antigens CD56 (1:50, clone HCD56, Biolegend) and CD29 (1:50 ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Unlabeled cells were then centrifuged at 300 x g and labeled with a cocktail of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer.
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bioRxiv - Cell Biology 2020Quote: ... followed by incubation on ice with labeling cocktail consisting of 1 μg/ml of PerCP-Cy5.5 anti-EPCAM antibody and Zombie Aqua viability stain (Biolegend 423101) diluted 1:400 from stock concentration in FACS buffer ...
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bioRxiv - Immunology 2021Quote: PBMCs were stained with the following antibodies and proteins for flow sorting: anti-CD19-APC (1:10 dilution, clone HIB19, cat. no. 982406, BioLegend), anti-IgD-FITC (1:20 dilution ...
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bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
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bioRxiv - Genetics 2021Quote: ... in 1 × phosphate-buffered saline with 0.1% Tween 20 (PBST) before being incubated with a 1:5000 dilution of anti-GAPDH-HRP (Biolegend, 649203) or 1:2000 dilution of anti-ACTIN-HRP (5125)
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bioRxiv - Microbiology 2021Quote: ... the cells were incubated in PBS-5% FCS on ice for 30 min with rabbit anti-K10 (1:1000) (Biolegend), and K10 was visualized with anti-rabbit AF488 (Life Technologies ...
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bioRxiv - Developmental Biology 2021Quote: ... and transferred to a 2 mL Eppendorf tube for immunostaining for 30 minutes on ice for the immune cells (CD45-PE/Cy7, 1:250, 103114, BioLegend), endothelial cells (ICAM2-A647 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in staining buffer (1% BGS-supplemented PBS) and incubated with anti-Flag-PE (clone L5; BioLegend #637310) and Ghost Dye Violet 510 (Tonbo Biosciences #13-0870-T100 ...
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...