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Citations for BioLegend :
7551 - 7600 of 8186 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... Cells were then stained with Brilliant Violet (BV) 421TM anti-human CD138 (Syndecan-1) antibody (BioLegend catalog #356516, clone MI15), FITC anti-human HLA-A ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339; 1 µg/ml anti-CD28 mAb, BioLegend, #102115) in the presence of 5 μg/mL Brefeldin A (BFA ...
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bioRxiv - Cancer Biology 2024Quote: ... The viability of T-ALL#1 cell suspension was assessed by flow cytometry with Zombie Aqua Viable dye (423102, BioLegend) prior to mouse injection ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 500 xg for 7 minutes and resuspended in 1 mL ice cold Cell Stain Buffer (CSB, BioLegend). Samples were filtered through a sterile pre-wet 70 μm mesh ...
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bioRxiv - Immunology 2024Quote: ... Cellular debris and red blood cells were removed by the Debris Removal Solution (Miltenyi) and 1× RBC Lysis Buffer (Biolegend), respectively ...
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bioRxiv - Immunology 2024Quote: ... and 0.75 ug of GAD and Empty tetramers in addition to 1:200 anti-human CD19-BV421 (BioLegend, Cat#363017) and 1:100 anti-human CD3-FITC (BioLegend ...
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bioRxiv - Developmental Biology 2024Quote: ... Single-cell suspensions were generated as described above with an additional incubation with anti-CD11b-BV421 (1:200, Biolegend #101251) and anti-Ly6g-AF647 (1:200 ...
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bioRxiv - Immunology 2024Quote: ... in the presence of anti-Ly6G AF488 (1A8) and anti-CD68 AF647 (FA-11) or their isotypes (1/100 each, Biolegend), and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... peptide-specific T cells were evaluated by FCM by staining with PE-conjugated MART-1(A27L) tetramer and anti-CD8 FITC (BioLegend) (Supplementary Table 1).
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bioRxiv - Immunology 2024Quote: ... It exhibits monocyte morphology and was originally derived from malignant cells obtained from the pleural effusion from a patient with histiocytic lymphoma.77 LFA-1 (CD11a/CD18) was assessed with a FITC anti-human antibody (clone m24, Biolegend), and MAC-1 (CD11b/CD18 ...
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bioRxiv - Microbiology 2024Quote: ... The cells were then incubated 0.1 % BSA in PBS with or without 1/40 dilution of PE/Cyanine7-conjugated anti-human IL6 (Biolegend, #501119) or 1/80 dilution of PE-conjugated anti-human IL8 (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in 100 µL of FACS Buffer and stained for 15 minutes at room temperature with an antibody cocktail containing anti-CD71 (1:50; PerCP/Cyanine 5.5; BioLegend, 334113), anti-CD235a (1:500 ...
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bioRxiv - Immunology 2024Quote: ... splenocyte and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 minutes at 4°C ...
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bioRxiv - Immunology 2024Quote: White blood cell and microglial cell Fc-receptors were blocked using a purified anti-CD16/CD32 antibody (1:200, clone 93, BioLegend) for 20 min at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 1 U/ml penicillin/streptomycin) with 3 ng/ml recombinant mouse GM-CSF (rGM-CSF; R&D Systems or BioLegend) at a concentration of 2.5 x 105 cells/ml in 150 x 15 mm petri plates at 37°C with 5% CO2 ...
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bioRxiv - Neuroscience 2024Quote: ... Slices were stained with Slices were stained with mouse anti-human Aβ1-16 antibody (6E10, Biolegend SIG-39320, 1:200) and detected using an IVIEW DAB Detection Kit (Ventana) ...
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bioRxiv - Immunology 2024Quote: Immune cells isolated above were stained with the following antibodies (Live/Dead Fixable NIR, Invitrogen, #L34975, 1:500; CD45, Pacific Blue, Biolegend, #103126 ...
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bioRxiv - Immunology 2024Quote: ... CD8+ T-cells from the mouse ear were sorted with a FACSAria sorter from samples treated with BHI-tween or HSV-1 and labeled with TotalSeqC hashtags antibodies (Biolegend). All samples were pooled together ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions were washed with PBS and resuspended in 100 μL Zombie aqua cell viability dye solution (1:400 prepared in PBS, Cat. No. 423101, BioLegend) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Supernatants were diluted 1:10 with 1% BSA ELISA assay diluent and ELISA was performed according to manufacturer’s instruction (ELISA MAX™ Standard Sets, BioLegend).
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bioRxiv - Cancer Biology 2024Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 µM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... Surface staining of the MDSC panel consisted of the following primary antibodies diluted in FACS buffer: anti-mouse CD45-Brilliant Violet 650 (1:200, 103151, Biolegend), anti-mouse F4/80-Brilliant Violet 785 (1:200 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cell suspension was blocked by incubating with Fc Block and immunostained with CD11b-Brilliant Violet 785 (1:1000; Cat# 101243, BioLegend), CD206-APC (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with a mouse anti-LAMP1 conjugated with Alexa fluor 647 (BioLegend, clone H4A3, Cat #328609, 1:500) for 1h ...
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bioRxiv - Immunology 2024Quote: ... The remaining cells were then plated at 1×106/mL density in IMDM medium containing 20 ng/mL mouse M-CSF (BioLegend) and cultured for 7-9 days without medium change to generate BMDMs.
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bioRxiv - Immunology 2024Quote: Single cell suspensions were washed with PBS and resuspended in 100 μL Zombie aqua cell viability dye solution (1:400 prepared in PBS, Cat. No. 423101, BioLegend) and incubated at room temperature for 15-30 minutes ...
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bioRxiv - Immunology 2024Quote: ... Then cells were incubated in the dark for 20 minutes with 50µL of the antibody mixture: 1:50 each of anti-CD3-AF488 (BioLegend 100210), anti-CD4-BV510 (BioLegend 100559) ...
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bioRxiv - Bioengineering 2024Quote: ... and vendor information of antibodies used for the flow panel in this study was as follows: (i) Live/Dead (BV510, 1:100, 423111; BioLegend), (ii ...
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bioRxiv - Immunology 2024Quote: ... Staining was performed by washing with 0.1% Triton-X 100 in PBS for 3 x 15 minutes then staining for CD3-PE (1:100, Biolegend, 100205) overnight at room temp ...
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bioRxiv - Systems Biology 2024Quote: ... after which the supernatant was discarded and 100µL of MojoSortTM Streptavidin Nanobeads (1:10 diluted in FACS buffer, Biolegend, 480016) was added to the mixture ...
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bioRxiv - Bioengineering 2024Quote: ... The following antibodies were used at a 1:100 dilution in Flow Cytometry Staining Buffer (eBiosciences #00-4222-26): CD45-PerCP Cy5.5 (Biolegend 103132), CD11b-Alexafluor 488 (thermofisher 53-0112-82) ...
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bioRxiv - Bioengineering 2024Quote: ... The following antibodies were used at a 1:100 dilution in Flow Cytometry Staining Buffer (eBiosciences #00-4222-26): CD45-PerCP Cy5.5 (Biolegend 103132), F4/80-FITC (Biolegend 157310) ...
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bioRxiv - Immunology 2024Quote: Aliquots of 5-10 x 106 cells were first assessed for viability staining using eFlour780 (eBioscience) fixable viability dye (1:1000) or 7AAD (Biolegend) viability dye (1:100) ...
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bioRxiv - Immunology 2024Quote: Cytokine levels were measured from culture media supernatants harvested 24 hours after plating using LegendPlexTM Human Inflammation Panel 1 (Biolegend), per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The cells were then washed twice with PBS before being incubated with Zombie NIR Fixable Viability dye (1:1000, BioLegend) for 15 min at RT ...
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bioRxiv - Physiology 2024Quote: ... The cell pellet was re-suspended in staining buffer supplemented with 1:100 anti-mouse CD16/32 (Fc block, BioLegend) and incubated on ice for 10 min ...
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bioRxiv - Bioengineering 2024Quote: Proapoptotic protein and cytokine release was measured in cell-free supernatants from 16h co-cultures of CAR-T and tumor cell lines at 1:1 E:T ratio and from 48h co-cultures of CAR-T and PDO at 10:1 E:T ratio using the Legendplex™ multi-plex bead-based immunoassay (cat. # 741187, Biolegend). For cytokine measurement ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining 80% cleared supernatant was transferred to a clean RNase-free tube and incubated with mouse anti-HA antibody (1:250; clone 16B12, Biolegend) on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads ...
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bioRxiv - Cancer Biology 2024Quote: ... blocked using 1% FBS diluted in permeabilization buffer for 15 mins and then stained with IFN-γ (Biolegend, clone: XMG1.2) and TNF-α (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: Lung cell suspensions prepared above were resuspended in 1 mL of PBS containing Zombie Green Viability Dye (0.1% Biolegend, 423112) and incubated at 4°C for 15 min ...
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bioRxiv - Cancer Biology 2024Quote: ... 250,000 cells were plated in 1 ml IMDM media with 20% FBS and 1% Penicillin Streptomycin containing 50 ng/ml recombinant mouse M-CSF (cat # 576406, Biolegend) in a 12-well plate ...
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bioRxiv - Cell Biology 2024Quote: ... hemolyzed lineage-positive and Sca-1-positive cells were labeled with biotin-conjugated antibodies and removed using MojoSort streptavidin nanobeads (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were then incubated on ice in the dark with APC anti-mouse Ly-6A/E (Sca-1) Antibody (clone D7, 108111, BioLegend) antibody for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... media was removed from cells and 45 µl of EMEM+1/100 Human TruStain FcX blocking solution (Fc Block, BioLegend) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were collected and washed three times with PBS and stained with 1:500 of FITC anti-γH2AX Phospho (Ser139) (BioLegend) antibody and Propidium iodide (1 mg/ml ...
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bioRxiv - Cancer Biology 2024Quote: ... were seeded in 48-well plate pre-coated with Purified anti-mouse CD3 (1:50 in PBS; clone 145-2c11; BioLegend) in RPMI supplemented with 10% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... at a dilution of 1:5000 followed by Fc receptor blocking using a combination of 0.5 µL per test of Purified Anti-Mouse CD16/CD32 Fc block (Tonbo) and 1 µL per test of Human Trustain FcX (Biolegend). Samples and fluorescence minus one controls were stained using antibodies at the dilutions listed in Table S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). The PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...