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Citations for BioLegend :
7401 - 7450 of 8186 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: After 7 days of shRNA-mediated knockdown 1 x 105 cells were wash once in PBS and resuspend in Annexin-V Binding Buffer (422201, BioLegend) with 3 μl of Annexin-V-APC (640941 ...
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bioRxiv - Immunology 2020Quote: ... Antibody dilutions in cell staining buffer containing 1 % TruStain fcX™ (anti-mouse CD16/32) Antibody (both BioLegend, London, UK) were used to stain according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... The sections were washed again 3 × 5 minutes in PBS and incubated overnight with rat anti-phospho-TDP primary antibody (1:100, 829901, BioLegend), in a dilution buffer (0.05% Tween ...
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bioRxiv - Cell Biology 2021Quote: ... Populations of interest based on DAPI negativity expression and lack of lineage markers (APC-Cy7; CD31, Ter119, CD45 at 1 μL/106 cells; Biolegend) expression were directly sorted into DMEM/F12 containing 10% FBS with 1% P/S/A ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 0.5 % BSA/PBS and incubated with 200 µL of a 1:50 dilution of hCD4-BV711 (OKT4, BioLegend, UK) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Effector function was assessed by stimulating cells for 4 hours with PMA (20 ng/ml) and Ionomycin (1 μg/ml) with GolgiStopTM and monitored for CD8-PerCP (RPA-T8, Biolegend), CD4-AF700 (RPA-T4 ...
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bioRxiv - Developmental Biology 2022Quote: ... MGs were localized using the mT/mG reporter or stained using a rabbit anti-K14 polyclonal antibody (1:500, 905301, BioLegend). Nuclei were counterstained with DAPI ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... to block nonspecific binding and then cells were stained using a master mix of CD45.1 (Biolegend Cat # 110728, 1:1000), F4/80 (Biolegend Cat # 123110 ...
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bioRxiv - Immunology 2022Quote: ... On day 6 BMDM were replated at 1 × 106 cells per well in 24-well plates and stimulated with 10 µg/ml Imiquimod (Biolegend) with 10 µg/ml anti-CD200R1 (OX131 ...
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bioRxiv - Immunology 2022Quote: ... The BM cells per animal were spined down and then resuspended in 1 mL Red Blood Cell Lysis (RBCL) buffer (Biolegend) at room temperature for 1 min to lyse the red blood cells ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2022Quote: ... at a 1:200 dilution for 30 mins at room temperature and was detected with anti-rabbit BV421 (Dnk, BioLegend). Flow was performed on Cytek Aurora (Cytek ...
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bioRxiv - Immunology 2022Quote: ... Subsequent iterative cycles of standard immunohistochemistry were performed using the following anti-human primary antibodies: podoplanin (D240, BioLegend; 1:100), LYVE-1 (rabbit polyclonal ...
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Orchestration of alternative splicing regulates bone marrow mesenchymal stem cells fate during agingbioRxiv - Cell Biology 2022Quote: ... We flushed bone marrow cells from the tibia and femora of male mice and incubated the cells with anti-Sca-1-PE (108108; BioLegend), anti-CD29-FITC (102206 ...
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bioRxiv - Systems Biology 2022Quote: ... The population corresponding to cluster B was sorted by top 1% of CD49c abundance via red florescent antibody (Biolegend 343808). Cells corresponding to cluster A were sorted from 25-75% abundance range ...
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bioRxiv - Cancer Biology 2022Quote: Cells were collected in suspension post digest and at each passage and were stained with a live/dead marker Zombie UV (1:1000, Biolegend) for 30 min at room temperature in DPBS (Gibco) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor sections were then incubated with fluorochrome-conjugated antibody (1:100, Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40 ...
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bioRxiv - Cancer Biology 2020Quote: ... Tumor sections were then incubated with fluorochrome-conjugated antibody (1:100, Alexa Fluor 488 anti-mouse F4/80, #123120, BioLegend; or PE anti-mouse CD40, #124610, BioLegend; or PE anti-iNOS ...
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bioRxiv - Bioengineering 2020Quote: ... Unbound cells were collected and cultured for 24 hours in the presence of 1 μg/ml anti-mouse CD3ε (145-2C11, BioLegend) and 0.5 μg/ml CD28 (37.51 ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Immunology 2021Quote: ... REA159), Brilliant Violet 421 (BV421)-conjugated anti-pSTAT3 (pY705, 13A3-1) or BV421-conjugated anti-pAKT (pS473, M89-61) (Biolegend) Abs.
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bioRxiv - Neuroscience 2020Quote: ... Cells were blocked in 10% BSA in PBS for 1 h at room temperature and then incubated with a MAP2 antibody diluted in 3% BSA (MAP2 chicken, 1:10000, Biolegend) for 60 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... and subsequently stained for 1 hr at room temperature with anti-H2AX-Phosphorylated (Ser139) antibody conjugated with Alexa Fluor 647 (Biolegend), or Anti-p-CHK1 antibody conjugated with PE (Cell Signaling ...
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bioRxiv - Genomics 2021Quote: Cells from the completed cTSA-seq reaction were resuspended in PBS containing 0.001% Tween-20 and Streptavidin conjugated to Alexa 488 (1:200, Biolegend, #405235) and an anti-rabbit secondary conjugated (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... was detected using biotin labelled anti-mouse IgG1 and IgG2a while total IgG (dilution 1:1000) was detected using anti-mouse HRP conjugated IgG followed by anti-mouse streptavidin-HRP (Biolegend). The plates were further incubated at 37°C for 1h and washed 7 times with a washing buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were resuspended for 30 min in 100 μL of flow cytometry buffer and anti-ICAM-1 (Pe-Cy7, Biolegend) and anti-VCAM (APC ...
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bioRxiv - Cancer Biology 2021Quote: ... in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11, PercPcy55, 1/300, Biolegend). Tumor cells (for RNA-seq ...
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bioRxiv - Cancer Biology 2020Quote: ... and labelled via incubation with 1:100 dilution of Alexa Fluor 488 conjugated anti-CD45 antibody (Cat. no. 304017, BioLegend) and 1:100 dilution of PECy7-conjugated anti-CD31 antibody (Cat ...
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bioRxiv - Cell Biology 2020Quote: Single cell suspension was generated from adult lungs and stained with antibodies: anti-EpCAM (APC-Cy7-conjugated, Biolegend,1:50), CD49F (APC-conjugated ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: Bone marrow cells from two 8-week-old wild-type mice were each stained with 1 μg of different hashtag antibodies (TotalSeq A0301 and TotalSeqA0302, respectively; BioLegend). At the same time ...
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with bead/Ab mixture for 1 hr at 37°C and then stained with PacificBlue-conjugated anti-CD66b (Biolegend) in PBS ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed and stained with 100 μl of 1:1000 dilution of fixable Zombie Aqua (BioLegend, San Diego, CA) for 20 minutes at 4°C in dark ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of PE-conjugated mouse anti-human IgG Fc antibody (BioLegend) on ice in dark for 1h ...
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bioRxiv - Immunology 2020Quote: ... buffer containing 1:100 dilution of target-specific antibodies (anti PD1:cat≠329905; Biolegend, and anti-PDL1: Cat≠329705; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2020Quote: ... The pellet was resuspended in flow cytometry staining buffer (FACS) buffer containing 1:100 dilution of CD3 antibody (Cat≠317307; Biolegend) and incubated for 30 minutes at 4°C in the dark ...
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bioRxiv - Immunology 2021Quote: ... and finally with a mixture of AlexaFluor 647-conjugated rat anti-mouse Ly6C antibody (BioLegend, clone HK1.4; diluted 1:500) and DAPI (Molecular Probes ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend), and intracellular staining of anti-human Arginase1 FITC (Ref ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation for 1h at RT in a 1:1000 dilution in blocking buffer of primary Ab (purified anti-Filaggrin polyclonal Ab, clone Poly19058, Biolegend). After washing ...
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bioRxiv - Immunology 2020Quote: ... lymph nodes were removed from Myc-eGFP mice and mashed and cell suspension activated with anti-CD3 (1 µg/mL, clone 145-2C11, Biolegend) and anti-CD28 (3 µg/mL ...
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bioRxiv - Immunology 2021Quote: ... at 4°C overnight with 70 µl/well of 1 µg/ml of a relevant capture mAb: purified anti-human IFN-γ (Clone:NIB42, BioLegend); Purified anti-human TNF-α (Clone:MAb1 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were transferred to a round-bottomed 96-well plate and were incubated with an AF647-conjugated anti-GFP antibody (1:20, BioLegend) and fixable viability dye eFluor 780 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... washed twice with True Nuclear Permeabilization/Wash buffer and then stained for 1 hour at room temperature with FoxP3 BV421 (206D, BioLegend), Ki-67 BV711 (Ki-67 ...
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bioRxiv - Genomics 2021Quote: ... Then cells were stained with the following cell surface marker antibodies for 30 min on ice (1:100 dilution, all from BioLegend): CD11b-PE (301306 ...
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bioRxiv - Immunology 2022Quote: ... propidium iodide (eBioscience) was added to a final dilution of 1:250 and Annexin V Alexa Fluor™ 647 (Biolegend) was used at 1:125 dilution ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
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bioRxiv - Immunology 2022Quote: ... PBMC collected 17 weeks post-boost were incubated for 10 min on ice with purified CD16 (3G8, BioLegend, 1:100), CD32 (FUN-2 ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in FACS buffer (1% BSA in PBS) at a concentration of 108 cells/mL and 1 μL negative CD8+ T cell biotin-antibody cocktail (Biolegend)/million cells was added and allowed to incubate for 15 min on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... is the complete list of antibodies used for experiments of T cell activation or exhaustion against primary AML: Anti-CD279 (PD-1) FITC (cat#329904 from Biolegend), CD366 (TIM-3 ...