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Citations for BioLegend :
6751 - 6800 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... The frequency of CD8+ CD57+ population in freshly isolated T cells was assessed by using PE-conjugated anti-CD8 (clone RPA-T8, Biolegend) and PerCP-conjugated anti-CD57 (clone HNK- ...
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bioRxiv - Immunology 2023Quote: ... Sip-T or single cell suspensions of tumor-infiltrating lymphocytes (TILs) were resuspended in PBS as 1-3×106 cells/ml and stained with fixable Zombie Aqua viability dye (BioLegend) as per manufacturer’s recommendation ...
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bioRxiv - Immunology 2023Quote: ... 5000 cells of each compartment were labeled with anti-mouse hashtag oligonucleotide (HTO) antibodies (TotalSeq™-C0301/C0302/C0303, Biolegend) and pooled together ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were we pelleted and washed once with AutoMACS buffer before resuspending AutoMACS with in DAPI (dead cell dye DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 422801, BioLegend) and DRAQ5 (DRAQ™ Live cell dye ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Bioengineering 2023Quote: ... whereas binding of T cell-secreted A4 monomer on OT-I T cell CD47 was assessed indirectly by staining with anti-mouse CD47 (miap301, Biolegend) at 4°C for 30min.
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bioRxiv - Cancer Biology 2023Quote: Secretion of CCL5 and CXCL10 by MSI and CIN CRCs into cell supernatants was analyzed using a custom LegendPlex cytokine bead array (BioLegend). Data was acquired on a CytoFlex S cytometer and analyzed in FlowJo.
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bioRxiv - Immunology 2023Quote: ... HLA-expressing cells were enriched with a positive magnetic sort using the biotinylated pan-HLA antibody (clone W6/32, Biolegend) in conjunction with the ThermoFisher CELLection Dynabead cell isolation kit ...
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bioRxiv - Synthetic Biology 2023Quote: ... OD600 of induced cultures was taken and used to determine approximate volumes of culture to stain two million cells with fluorescently labeled antibodies (anti-FLAG PE, Biolegend, cat# 637309 ...
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bioRxiv - Immunology 2023Quote: ... with the exception of CD62L which was replaced by a Zombie Yellow cell viability stain (BioLegend, San Diego, CA, USA) (see Sup ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor of the cells was blocked using an anti-mouse CD16/32 antibody (Fcγ R III/II, Ly-17; BioLegend). After 30 min of incubation at 4°C cells were washed with FACS Buffer (PBS containing 1% FBS ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions from the spleen were prepared as described above and splenocytes were exposed to 2-μM Tag-it VioletTM Proliferation and Cell Tracking Dye (Biolegend) diluted in PBS supplemented with 0.1% bovine serum albumin (BSA ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: Harvested lungs and spleen immune cells were incubated with recommended dilutions of antibodies conjugated to fluorophores: CD3-BV510 (BioLegend, #100234), CD4-BV785 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were activated for 3 h in a round-bottom 96-well plate using Cell Activation Cocktail (with Brefeldin A) (BioLegend). Plates were then centrifuged at 300 x g for 5 min and the pellets resuspended in 50 µL of Cell-ID™ Cisplatin-195Pt viability reagent (Standard BioTools) ...
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bioRxiv - Immunology 2023Quote: ... electroporated Jurkat cells were interrogated by pMHC-multimer staining with the following surface panel: anti-CD3 APC Fire 50 (SK7, Biolegend), anti-CD8 Pacific Blue™ (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... Stained T-IEL and unstained lymph node T cells were mixed at a 1:1 ratio before stimulation with 30 μg/ml anti-CD3 (Clone 145-2C11, Biolegend) crosslinked with 5 μg/ml anti-Hamster (Jackson Immunoresearch) ...
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bioRxiv - Immunology 2023Quote: ... CD4 and CD8β cells were depleted from the T-IEL compartment using CD4 and CD8β biotinylated antibodies and magnetic streptavidin nanobeads (MojoSort) (Biolegend). CD8α+ cells were purified from CD4 and CD8β depleted T-IEL and lymph node T cells using the CD8α Positive Selection Kit (Stemcell Technologies) ...
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bioRxiv - Immunology 2023Quote: ... the cells were stained as described for dSTORM analysis with antibodies against scFv-Linker (evitria) or CD20 antibody (clone 2H7, Biolegend). Samples were stored at 4°C until imaging.
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bioRxiv - Immunology 2023Quote: ... plus eBioscience Fixable Viability Dye eFluor 780 or eFluor 506 to distinguish live and dead cells and with anti-CD16/CD32 (clone 93, BioLegend) to prevent non-specific antibody binding ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Cancer Biology 2023Quote: ... EMT phenotype of in vivo isolated tumor cells was analyzed using the same antibody panel implemented with anti-mouse CD45 (30-F11; Biolegend), CD90.1 (OX-7 ...
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bioRxiv - Cancer Biology 2023Quote: ... The FCM panel used to analyze EMT phenotype of in vitro tumor cells relied on the use of the anti-mouse pancytokeratins (C-11; Biolegend), CD73 (TY/11.8 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then diluted with 50 μL PBS + 2% FBS and FITC anti-CD45 (BioLegend, 304005; 1:1,000 final dilution) and incubated for 20 min on ice in the dark ...
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bioRxiv - Immunology 2023Quote: ... Cells were collected and stained with fluorescently labeled antibodies against CD45 PE-Cy 7 (clone: 30-F11, cat # 103114: Biolegend), TCRβ APC (clone ...
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bioRxiv - Immunology 2023Quote: ... cells were stained with Zombie Near IR at a 1:1000 dilution in PBS following the manufacturer’s instructions (Biolegend, #423117). Cells were then fixed ...
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bioRxiv - Genomics 2023Quote: ... anti-CD6 or anti-CD27) were sorted on the basis of live single cells that are CD69+ (PE-Dazzle 594, Biolegend). Sorted post-activation naïve and memory T cells were counted.
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bioRxiv - Cell Biology 2023Quote: ... Long bones and iliac crests were isolated and bone marrow was obtained by centrifugation at 2500g for 40s as previously described.41 Hematopoietic stem and progenitor cells (HSPCs) were separated by lineage depletion using an antibody mixture (Lineage depletion panel, 133307, Biolegend) and magnetic beads (CD4 untouched ...
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bioRxiv - Biochemistry 2023Quote: ... was added 1 day after serum deprivation and cell viability was assessed the following day by using Zombie Fixable Viability Kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... Isolated nuclei were loaded on a Chromium 10X genomics controller following the manufacturer protocol using the chromium single-cell v3 chemistry and single indexing and the adapted protocol by Biolegend for the HTO library preparation ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... 0.5-1×106 bone marrow cells were stained with the following surface antibodies (2 panels) against: CD14 (Biolegend, M5E2, AF700), HLA-DR (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Immunology 2023Quote: ... and cells without either EpCam or CD45 markers were sorted using antibodies against EpCam (clone G8.8, Biolegend, San Diego, CA) or CD45 (clone 30-F11 ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were first processed for surface marker labeling by incubation with anti-CD8-BV421 and anti-CD4-BV650 (Biolegend) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Cells were then pelleted and resuspended in 100 µL of Zombie Green viability dye (1:500 in PBS; BioLegend, 423111). After 15 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and resuspended in flow buffer for intracellular staining (buffer stock diluted in deionized water (Biolegend, 421002-BL) supplemented with 0.3% v/v BSA (Sigma-Aldrich ...
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bioRxiv - Genomics 2023Quote: ... Fluorophore conjugated antibodies against the following antigens were used for input and day 3 cells (All Biolegend unless otherwise noted): panel 1 ...
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bioRxiv - Immunology 2023Quote: ... The cells were resuspended in 1 ml R10 and 1-2 million cells were plated in 1ml R10 with 1 μl Brefeldin A (BioLegend) for 4 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Cancer Biology 2023Quote: ... PD-L1 expression on KPC cells was assessed with an allophycocyanin-conjugated anti-mouse PD-L1 Ab (clone 10F.9G2; BioLegend). Flow cytometry was performed using a Cytek Aurora (Cytek ...
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bioRxiv - Cancer Biology 2023Quote: ... The collected cells were fixed with 70% ethanol and stained with 50 μg/mL propidium iodide (BioLegend, San Diego, CA) and 100 μg/mL RNAse A (Thermo Fisher) ...
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bioRxiv - Cancer Biology 2023Quote: ... The isolated single-cell suspension and peripheral leukocytes were fluorescently stained with anti-mouse CD11b-FITC (1:200, BioLegend, 101205) and Ly6G-PE (1:80 ...
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bioRxiv - Cancer Biology 2023Quote: ... 90% of the thawed cells (>200 x 106) were stained with biotinylated antibodies against CD45 (1:50, clone HI30; Biolegend) and CD235a (1:50 ...
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bioRxiv - Immunology 2023Quote: ... Supernatants were collected at d5 of the culture for the analysis of T cell cytokine production using a combination of commercially available antibodies (Biolegend). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Dead cells were stained using 40 µl/well of a cocktail of Annexin V – FITC (#640906; Biolegend, 1:50 dilution) and 7 AAD (#420404 ...