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Citations for BioLegend :
6701 - 6750 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were washed two times with PBS-Tw and 3,3’,5,5’-tetramethylbenzidine peroxidase (TMB) substrate (BioLegend; San Diego, CA, USA) was added ...
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bioRxiv - Cancer Biology 2024Quote: ... tumour cell lines were stained with Zombie Aqua Fixable viability dye (1:1000 in PBS) and for NK ligand (Biolegend) expression in PBS containing 5% FBS and 0.1% sodium azide for 20-30 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... The BALF was processed to isolate the cells upon RBC lysis and stained with PE anti-mouse Ly6G clone 1A8 (Biolegend) and FITC anti-mouse F4/80 clone BM8 (Biolegend ...
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bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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bioRxiv - Immunology 2023Quote: ... CD4+ and CD8+ T cells from PBMC were stained with the following cell surface markers in MACS buffer at room temperature for 20 mins: CD3-AF488 (clone OKT3, Biolegend), CD14-eFluor450 (clone 61D3 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were counted and then incubated at 4 °C for 25 min with FITC anti-mouse CD45 (Biolegend, 103107, USA), Brilliant Violet 650 Ly6G (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... FL or bone marrow HSPC were cultured on OP9-DL1 cells in the presence of 1 ng/mL IL-7 and 5 ng/mL FLT3L (BioLegend) as previously described 70 ...
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bioRxiv - Neuroscience 2024Quote: ... FluoSphere- containing media was then removed from the wells and cells were processed for flow cytometry using markers for CD11b (1:200, 101206, Biolegend) and CD45 (1:200,103132 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells from the epithelial layer were sorted gating on single positive for CD326-PE/Cy7 (BioLegend, clone DECMA-1, #147310) and CD104-AF647 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were then washed twice in FACS buffer (PBS + 2% FBS + 1mM EDTA) before staining with anti-pERK1/2 (Biolegend 369506 100x ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed twice with flow buffer prior to staining with BV421 Annexin V (BioLegend, Cat#141703; 2.5 μl/sample) in Annexin binding buffer (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... 106 of the CTV labeled T cells were stimulated in vitro for 3 or 5 days in cultures containing 100ng/ml IL-15 (BioLegend) or a combination of 100ng/ml IL-7 (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... Cell Hashing was used to label samples from distinct samples.46 Single cell suspensions were labeled with the TotalSeq Hashtag Antibodies (BioLegend) for 30 min on ice ...
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bioRxiv - Genomics 2024Quote: ... amplification and sequencing libraries were generated using the Next GEM Single Cell 3’ Kit v3.1 (10X Genomics) with the recommended modifications for compatibility with the TotalSeq-A cell hashing and CITE-seq reagents (BioLegend). Organ donor GEX libraries were sequenced on a NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2024Quote: Secretion of soluble cytokines and chemokines were assayed from cell-free culture supernatants of in vitro stimulated neutrophils and monocytes using bead-based immunoassays (LegendPlex, Biolegend). Two assays were used allowing the quantification of IL-1β ...
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bioRxiv - Immunology 2024Quote: ... the cells were stained with a live/dead fixable dye (Zombie Red dye, 1/800 dilution – BioLegend, San Diego, CA) for 20 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... cells were washed again and stained with anti-mouse antibodies to intracellular markers anti-TNF-α-Alexa Flour 700 (Biolegend, Alexa Fluor® 700 anti-mouse TNF-α Antibody ...
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bioRxiv - Immunology 2024Quote: ... Live cells or nuclei were gated based on their forward and side scatter followed by Zombie NIR fixable (Biolegend 423106), Fixable Viability Dye eF780 (eBioScience 65086514) ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... then cells were stained with monoclonal antibodies for 30 min at 4°C in Brilliant Stain Buffer (BioLegend, cat. #563794). See supplementary information for details on flow cytometry antibodies ...
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bioRxiv - Immunology 2024Quote: To differentiate between live and dead cells were firstly stained with the Zombie NIR™ Fixable Viability Kit (BioLegend, 423106), the Zombie Yellow™ Fixable Viability Kit (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2023Quote: ... The hCMV-peptide pool stimulated cells from each donor were washed and individually stained with Cell-hashtag (HTO:Hash Tag Oligo) TotalSeq C antibody (0.5 µg/condition; Biolegend, Table S2B) as per manufacturer’s recommendations for 30 minutes on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... mesothelioma cell lines were further pre-treated with anti-MHC class I antibody (10 μg/ml) (Clone W6/32, Biolegend) for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tumor cells were stained with AF488 anti-CD8 antibody and APC anti-CD11c antibody (BioLegend, San Diego, CA) and subjected to flow cytometry analysis to access infiltrated immune cells in the tumor microenvironment ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse lung single cells were similarly stained but with a cocktail of antibodies (1:250 each) against CD45-PE/Cy7 (103114, BioLegend), Icam2-A647 (A15452 ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... Dead cells were stained using 40 µl/well of a cocktail of Annexin V – FITC (#640906; Biolegend, 1:50 dilution) and 7 AAD (#420404 ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained for sgRNA expression with an APC conjugated anti-mouse CD90.1/Thy1.1 antibody (no. 202526, BioLegend; 1:400) and Human TruStain FcX Fc receptor blocking solution (no ...
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bioRxiv - Immunology 2022Quote: ... Cells were stained with a staining mix containing Fc receptor blocker (TruStain fcX™, BioLegend, Amsterdam, The Netherlands, 1:100), viability stain (Zombie Aqua™ and Zombie Green™ Fixable Viability Kit ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...
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bioRxiv - Genomics 2023Quote: ... control samples (Mouse IgG2a) were sorted on the basis of live single cells that are CD69− (PE-Dazzle 594, Biolegend) and the stimulated samples (anti-CD3 plus either anti-CD28 ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were centrifuged and resuspended in flow buffer for intracellular staining (buffer stock diluted in deionized water (Biolegend, 421002-BL) supplemented with 0.3% v/v BSA (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... The phenotype of differentiated HL-60 cells was assessed by flow cytometry using mouse antihuman CD35 PE conjugated antibody (BioLegend) and mouse anti-human CD71 APC conjugated antibody (BioLegend) ...
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bioRxiv - Immunology 2023Quote: ... Peripheral blood was collected via retro-orbital sinus sampling from mice anesthetized with inhalational isoflurane and red blood cells were lysed with RBC Lysis Buffer (BioLegend) according to the manufacturer’s protocols ...
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Neuroscience 2023Quote: Differentiated cortical cells were validated for neuronal and cortical markers by immunostaining using antibodies for TUBB3 (BioLegend, Cat#MMS-435P), TBR1 (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...
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bioRxiv - Immunology 2023Quote: ... Murine cells were stained for 30 min at 4°C with the following antibodies (all from Biolegend, unless otherwise stated): CD3 APC(Clone:17A2 Cat:100236 ...
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bioRxiv - Pathology 2023Quote: ... Single cell suspension was stained with antiCD45:Pacific blue (368539, clone 2D1, Biolegend, San Diego, CA, USA, 1:200 dilution), anti-EpCAM:BV650 (324225 ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed 2 times then resuspended in 100 μL wash buffer with 0.1 μg/mL streptavidin-PE (Biolegend 405203) and incubated on ice for 15 min ...
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bioRxiv - Immunology 2023Quote: CAR-NK92 cells and NK92 cells were suspended with fresh culture medium supplied with 2 nM monensin and anti-CD107a-PE antibody (BioLegend) (1:1000 dilution) ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were cocultured with NALM6GL cells at indicate E/T ratio for 4 hours with the presence of 1x Brefeldin A (Biolegend). Cells were first stained with the Live/Dead-NearIR dye before fixed ...
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bioRxiv - Immunology 2023Quote: ... Intracellular cytokine stain was performed following restimulation of pmel-1 T cells as above with the addition of 1X Brefeldin A (Biolegend) and 1X Monensin (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... 2 % FCS (1 to 5 x107 cells/500 μl) and incubated with the following mAbs: CD45 AF700 (Biolegend, clone HI30), CD34 PB (Biolegend ...