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Citations for BioLegend :
5751 - 5800 of 7138 citations for Primary Human Melanocyte Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... after which time cells were stained with 5 μg/ml CD8-APC (clone SK1; Biolegend, UK), harvested and CSFE dilution measured by flow cytometry ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were analyzed by fixing and staining with intranuclear antihuman mAb FOXP3-PE antibody (BioLegend, CA) and counted for cells that are positive for CFSE dye intensity ...
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bioRxiv - Genomics 2019Quote: ... Stained cells were washed thrice with Flow buffer and 7-AAD viability dye (3-5µl, BioLegend) was added before quantification ...
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bioRxiv - Molecular Biology 2021Quote: ... Cells were harvested and immediately processed with the Biolegend Phase-Flow Alexa Fluor 647 kit (BioLegend). Cells were counterstained with DAPI and at least 10 000 events were acquired on a BD Fortessa (Becton Dickinson).
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bioRxiv - Cell Biology 2020Quote: ... Single-cell WBM suspensions were surface-stained with fluorophore-conjugated antibodies raised against CD31 (390; Biolegend) and CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed twice with PBS before staining with viability dyes Zombie NIR (BioLegend, catalogue# 423105) or LIVE/DEAD Fixable Blue (Thermo Fisher ...
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bioRxiv - Systems Biology 2021Quote: ... centrifuged and resuspended in 5 ml of 1X red blood cell (RBC) lysis buffer (420301, BioLegend) for 5 min on ice ...
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bioRxiv - Cell Biology 2020Quote: ... cells were stained in PBS containing 2% (w/v) BSA and the appropriate antibodies from Biolegend. Foxp3 expression was performed using the Foxp3 staining kit from eBioscience ...
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bioRxiv - Cell Biology 2020Quote: Cell death was detected with the FITC Annexin V Apoptosis Detection kit (BioLegend, Cat No. 640922). Infections were performed as described above with mCherry-Stm at an MOI of 100 ...
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bioRxiv - Immunology 2021Quote: ... and T cell phenotype was assessed by antibodies against CD25 (1:100, AF488, BC96, 302616, BioLegend), CD127 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Cells were immunostained for 25 minutes at room temperature with anti-CD56 (BV421; clone HCD56, Biolegend) or anti-CD55 (PE ...
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The skin environment controls local dendritic cell differentiation and function through innate IL-13bioRxiv - Immunology 2021Quote: ... Cells were then fixed and permeabilized using a True-Nuclear™ Transcription Factor Buffer Set (BioLegend) and stained with anti-RORγt (Q31-378 ...
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bioRxiv - Immunology 2020Quote: ... Single-cell suspensions were treated with CD16/32 Fc blocking antibodies (Biolegend; catalog 101310; clone 93) to prevent non-specific antibody binding and stained with the fluorescent-labeled antibodies for 20 minutes followed by fixation using 1% paraformaldehyde ...
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bioRxiv - Immunology 2021Quote: ... with half undergoing naïve CD4+ T cells isolation using MoJo magnetic bead negative selection kits (BioLegend), and the other half undergoing CD90+ cell depletion (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... the cells were incubated for 15min on ice with MojoSortTM Streptavidin Nanobeads (50µl/10×107, BioLegend). After an additional washing step ...
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bioRxiv - Immunology 2021Quote: ... cells were first washed in PBS and then incubated with Zombie NIR fixable viability dye (Biolegend) for 10mins at room temperature protected from light before following the approach detailed above ...
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bioRxiv - Immunology 2020Quote: ... T cells were stained with anti-CD4 Alexa 700 (clone OKT4, Biolegend, Cat. 317626, 1:100), anti-CD27 PerCP5.5 (clone M-T271 ...
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bioRxiv - Immunology 2021Quote: ... an equal number of cells were first incubated with 0.025 μg anti-CD16/32 (2.4G2; Biolegend) for 10mins on ice to block Fc receptors and then stained with a combination of the antibodies detailed in Supplementary Table 4 ...
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bioRxiv - Immunology 2021Quote: ... The antibodies used for identification of CD8 T-cells were anti-CD3 (APC, clone UCHT1, Biolegend) and CD8 (PerCP/Cyanine5.5 ...
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bioRxiv - Immunology 2021Quote: ... cells were stained in FACS-PBS with anti-CD8-BV711 (1:300, clone 53-6.7, BioLegend), anti-CD4-SB600 (1:1000 ...
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bioRxiv - Genetics 2020Quote: ... Cells were stimulated for 3 hours with 2.5 μg/ml of anti-CD3 (Biolegend, catalog 317315) and anti-CD28 (Biolegend ...
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bioRxiv - Genetics 2020Quote: ... For staining cells were washed twice with Intracellular Staining Permeabilization Wash Buffer (Biolegend catalog No. 421002), and re-suspended in same buffer with anti-VDAC-1 (abcam catalog No.15895 ...
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bioRxiv - Cancer Biology 2021Quote: ... then cells were stained with antibodies for 30 mins on ice: biotinylated anti-PDPN (BioLegend 127404), biotinylated anti-PDGFRα (anti-CD140a ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were assessed using the APC AnnexinV Apoptosis Detection Kit with PI (BioLegend, CA, USA; #640932) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: Reagents and antibodies used for flow cytometry and cell sorting: Zombie Violet™ fixable dye (BioLegend), CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2022Quote: ... Cells from different mice were stained with the following TotalSeq-A hashtag antibodies (all from BioLegend): Hashtag 1 (A0301) ...
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bioRxiv - Immunology 2022Quote: ... The cells were then washed in cold PBS and resuspended in RBC lysis buffer (BioLegend, 420301) for 1 min at 37°C ...
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bioRxiv - Neuroscience 2022Quote: ... The resuspended cell pellet was stained for anti-mouse CD3-PE (1:200, 145-2C11 Biolegend), anti-mouse CD45-APC/Cy7 (1:200 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were either first stained with either Zombie UV or Zombie NIR fixable viability dyes (BioLegend) (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... in B cell medium in the presence or absence of 10 μg/ml anti-CD86 (BioLegend) or 1 μg/ml CD40L (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated in PBS containing Zombie UV™ Fixable Viability Dye (BioLegend, San Diego, CA) at 4 °C for 20 min.
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bioRxiv - Cancer Biology 2022Quote: ... B cell depletion was done by a monthly intravenous injection of anti-CD20 (Clone SA271G2, BioLegend), starting one week prior to orthotopic transplantation of cells.
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bioRxiv - Developmental Biology 2022Quote: ... These suspended single cells were stained with Ter119-Alexafluor-647 (Biolegend, Catalog# 116218, 1:100 dilution) for 30 minutes at 4° Celsius ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for cell sorting experiments: anti-CD49f-APC (1:300, 313615, BioLegend), anti-CD49f-PercP/Cy5.5 (1:300 ...
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bioRxiv - Cell Biology 2022Quote: ... The following antibodies were used for cell cycle analysis: anti-CD49f-FITC (1:200, 313606, BioLegend), anti-Sca1-Ly-6A/E-Violet 605 (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... lysed for 30sec at room temperature in red blood cell lysis buffer (Biolegend, catalog no. 420301), washed with ice-cold PBS-EDTA and centrifuged at 1300rpm for 10min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... Negative cells were sorted based on the absence of B2M-PE antibody staining (clone 2M2, BioLegend) using magnetic separation with PE-beads (Miltenyi) ...
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bioRxiv - Developmental Biology 2022Quote: ... live GFP+ cells (Extended Data Fig.1; live stain 1:500 propidium iodide solution (BioLegend #421301)) were sorted on an FACSAria III (BD ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions were treated with CD16/32 Fc blocking antibodies (Biolegend; catalog 101310; clone 93) to prevent non-specific antibody binding and stained with the fluorescent-labeled antibodies for 20 minutes ...
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bioRxiv - Immunology 2022Quote: ... cells were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (Biolegend) for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cells were filtered through a 70 µm strainer and stained with the following antibodies from Biolegend: α6-integrin-PE (1:1,000) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were stained for 15 min in the dark with 5 μL APC Annexin V (Biolegend) and 5 μL 7-AAD Viability Staining Solution (Biolegend) ...
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bioRxiv - Immunology 2022Quote: ... CAR CD8+ T cells were identified with CD3-APC/Cy7 or CD8-Alexa 700 antibodies (Biolegend). Activation of CAR-MAIT and CAR CD8+ T cells were determined with CD25 staining using CD25-APC antibody (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... GC B cells stained using rat anti-GL7 antibody (FITC; BioLegend, catalog no. 144604, 1:250), Tfh cells stained using anti-CD4 antibody (BioLegend ...
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bioRxiv - Neuroscience 2022Quote: ... red blood cells (RBCs) were removed by lysis in 2 mL of RBC lysis buffer (BioLegend) for 3 minutes ...
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bioRxiv - Immunology 2022Quote: ... Isolated cells were stained with Zombie NIR and CD4 Pacific Blue (both Biolegend, San Diego, USA) and were acquired on BD LSRFortessa (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... Cells were activated using Dynabeads M-280 Tosylactivated magnetic beads coated with αCD3 (clone OKT3, Biolegend) and either αCD28 (clone CD28.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were stained with antibodies of cytokines (anti-IFN-gamma [Biolegend, 505808], anti-IL-2 [Biolegend, 503822] ...
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bioRxiv - Immunology 2022Quote: ... Colon cells were either untreated or treated with 25 µg/ml recombinant mouse IFN-β1 (Biolegend.) Supernatant was collected for ELISA and cells were stained for flow.
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: Cells were counted and stained with anti-mouse Epcam CD326-AlexaFluor647 antibody (1:100, Biolegend, 118212), CD31-APC (1:250 ...