Labshake search
Citations for Roche :
4851 - 4900 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... all multiplexed single-cell libraries were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche, 50-196-5234). For each processing batch (n = 2) ...
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bioRxiv - Physiology 2024Quote: ... cDNA was synthesized using the qRT-PCR Kit (Toyobo, FSQ-101) and the quantitative reverse-transcriptase PCR (qRT-PCR) was conducted using FastStart SYBR Green Master (Roche) on a LightCycler qPCR system (Bio-Rad) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis and RNA-seq library preparation were constructed either using the KAPA stranded RNA-seq library preparation kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Nanopore compatible libraries were constructed using either the ONT SQK-LSK109 or ONT SQK-LSK114 kit (Oxford Nanopore Technologies, Oxford, UK) according to the manufacturer’s instructions except that Kapa pure beads (Roche, UK) were used to perform the clean up steps rather than Ampure XP beads and without applying any fragmentation step to the DNA.
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bioRxiv - Bioengineering 2024Quote: ... Only RNA samples with an RNA Integrity Number (RIN) above 8.0 were selected for library construction using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche Diagnostics). The adapter ligation step of library preparation involved a 1:10 dilution of NEBNext® Multiplex Oligos for Illumina® Sets 1 ...
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bioRxiv - Immunology 2024Quote: ... Quantitative PCR (qPCR) was performed using a SensiFAST™ SYBR® No-ROX Kit (Roche Bioline, cat. no. BIO-98020). Expression levels of all genes were normalized to the geometric mean of housekeeping genes ...
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bioRxiv - Developmental Biology 2024Quote: ... and 7SL were amplified from RT-PCR of testicular RNA using the primers containing T7 and T3 promoter sequence (Supplemental Table S5) and used for cRNA probe synthesis with T3 RNA polymerase using DIG RNA labeling kit (Roche). Signals of 45S pre-rRNA ...
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bioRxiv - Biophysics 2024Quote: ... genomic DNA from individual larvae or fin-clipped tail fins were extracted using the Kapa Express Extract kit (Kapa Biosystems) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of total RNA from each sample was converted into cDNA with First Strand cDNA Synthesis Kit (Roche, #04897030001). The qPCR was performed using LightCycler 480 SYBR Green I Master (Roche ...
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bioRxiv - Genomics 2024Quote: ... Library preparation was performed using the Kapa Hyper Prep library preparation reagents following the manufacturer’s protocol (Kapa Hyper Prep Kit, Roche 07962363001). ChIP libraries were sequenced by the McGill University Genome Centre using the Illumina HiSeq 4000 at 50 bp single reads or NovaSeq 6000 at 100 bp single reads.
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bioRxiv - Genomics 2024Quote: ... and subsequent buffer (Reagent W2, Buffer W3 and Enzyme W4) were replaced with KAPA HiFi HotStart Uracil+ Ready Mix Kit (Roche), which included an uracil tolerant polymerase ...
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bioRxiv - Genomics 2024Quote: ... or the MagNA Pure 96 DNA and Viral NA Small Volume Kit automated on the Magna Pure 96 (Roche, Switzerland) (SSI and L&F) ...
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bioRxiv - Developmental Biology 2024Quote: Retinal and corneal cell death was assessed using the TUNEL Fluorescent In Situ Cell Death Detection Kit (Roche, Mannheim, Germany) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... 20-250 ng of genomic DNA was prepared for sequencing using the KAPA HyperPrep Kit with Library Amplification (Roche, KK8504). Target enrichment with a customized bait panel targeting 538 cancer-relevant genes (Table S4 ...
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bioRxiv - Zoology 2024Quote: Virus nucleic acid was isolated using the Roche High Pure viral nucleic acid extraction kit (Roche Diagnostics GmbH, Manheim, Germany). The extraction process involved adding 200 μl of the working binding buffer (binding buffer mixed with poly[A] carrier RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic libraries from approximately 500 ng gDNA were prepared using the standard KAPA paired-end sample preparation kit (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... gene expression (5’Gex) and TCR libraries were generated then quantified via Agilent Technologies 4200 Tapestation and KAPA library quantification kit (Roche). Targeted read depth included 25,000 reads per cell for gene expression libraries and 5,000 reads per cell for TCR libraries ...
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bioRxiv - Genetics 2024Quote: ... the origin of replication and the ampicillin resistance gene from pUC19 and the terminator from the pCAG mammalian expression vector were PCR amplified with KAPA HiFi HotStart ReadyMix PCR Kit (Roche). The lentiviral pLG1 library vector (Addgene #217306 ...
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bioRxiv - Cell Biology 2024Quote: ... The reaction was performed by using using SYBR green kit (11201ES03, Yeasen) on a LightCycler 480 system (Roche, Penzberg, Germany). The primers used were as follows ...
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bioRxiv - Biochemistry 2024Quote: IL-2 plasmids were mutated by PCR amplification using primers containing the mutations of interest the KAPA HiFi HotStart ReadyMix PCR kit (Roche) according to the manufacturer’s recommendations ...
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bioRxiv - Biochemistry 2024Quote: DNA from 1×106 cells of permanently transfected and non-transfected HEK293 cells was extracted using the High Pure PCR Template Preparation Kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used a Covaris E220 sonicator to shear DNA and we then prepared genomic libraries using the KAPA HyperPlus kit (Roche). Individual libraries were indexed using KAPA UDI’s and pooled libraries were sent to Novogene for Illumina paired-end 150 bp sequencing on a Novaseq X.
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bioRxiv - Microbiology 2020Quote: ... the DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Cancer Biology 2021Quote: ... Library qualities and sizes were checked with the TapeStation 4200 and then quantified using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems). Libraries were sequenced on the Illumina NextSeq500 sequencer to a depth of about 50 millions of 75-bp pair reads per library.
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bioRxiv - Cancer Biology 2021Quote: ... RT-PCR was performed using first strand synthesis with specific primers (Reagents) and SYBR FAST qPCR Kit (Kapa Biosystems, Wilmington, MA) in a StepOne cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Microbiology 2020Quote: ... The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA Illumina Library prep kit (KAPA biosystems). Libraries for the rest of the samples were prepared in 96-well plates ...
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bioRxiv - Developmental Biology 2021Quote: ... Illumina-compatible sequencing libraries were prepared by using a modified version of the KAPA HyperPrep library kit (KAPA BioSystems, Willmington, MA). Quality check steps were performed to assess the fraction of proximally ligated DNA labeled with biotin ...
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bioRxiv - Cell Biology 2020Quote: ... Each RNA-seq library was analyzed by the fragment analyzer (Advanced Analytical Technologies) and quantified by KAPA Library Quantification Kit (KAPA Biosystems) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2020Quote: ... Quantitative PCR reactions were performed with fast Start Universal Probe Master kit and UPL-probe system in a LightCycler 480 Instrument (Roche Diagnostics) as recommended by the manufacturer ...
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bioRxiv - Developmental Biology 2021Quote: ... with on-column DNase digestion followed by cDNA synthesis using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Diagnostics, 04379012001) using random primers ...
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bioRxiv - Immunology 2021Quote: Approximately 200-500 ng of FFPE RNA extracted from FFPE slides with a DV200 range between 3-99 or 65-100 ng of fresh frozen RNA (DV200 98-99) per sample were used for RNA library construction using the KAPA RNA Hyper library prep kit (Roche, Switzerland). The number of pre-capture PCR cycles was adjusted based on the quality and quantity of RNA extracted from the samples ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... The library size distribution was checked on an Agilent high-sensitivity DNA chip and the library quantified using the KAPA library quantification kit (KAPA Biosystems). For each reaction ...
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bioRxiv - Genetics 2021Quote: ... PCR products were cleaned using Ampure XP Beads (Agencourt) and quantified using the KAPA Library Quantification Kit for Illumina® Platforms (Roche). Libraries were sequenced on a NextSeq sequencer (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... and samples with RIN >7 were used for paired-end sequence library construction using the KAPA mRNA HyperPrep Kit (Roche # 08098123702). For ChIP samples ...
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bioRxiv - Developmental Biology 2021Quote: ... mRNA was enriched by poly-A capture and mRNA-seq libraries were generated using KAPA Stranded mRNA-seq Kit (KAPA Biosystems). Libraries were generated from two biological replicates ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Synthetic Biology 2022Quote: ... The PCR products from each level were pooled in equimolar concentration and the pool was converted into a sequence-ready library with the Kapa Hyper Library Construction kit (Roche, CA) with no PCR amplification ...
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bioRxiv - Molecular Biology 2020Quote: ... The entire ChIP DNA or 10 ng DNA in the case of ChIP input was used to make DNA library with the KAPA Hyper Prep Kit (KAPA Biosystems) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was synthesized from 1 μg total RNA for each sample using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche, 05081963001), with a mixture of oligo dT and random primers ...
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bioRxiv - Genomics 2020Quote: ... The libraries were visualized with the Fragment Analyzer (Advanced Analytical Technologies) and quantified using the Kapa library quantification kit for Illumina platforms (Kapa Biosystems). [3] Based on the concentration ...
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bioRxiv - Immunology 2021Quote: ... and used as a template for the synthesis of cDNA utilizing the Transcriptor First Strand cDNA Synthesis Kit (Roche, Basel, Switzerland). Total genomic DNA was extracted by boiling BMDCs in a 50 mM NaOH solution as previously described (47) ...
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bioRxiv - Microbiology 2020Quote: ... cDNA was synthesized with 12 µL of RNA and anchored oligo(dT)18 primer in a 20 µL reaction using the Transcriptor First Strand cDNA synthesis kit at 55°C for 30 min for reverse transcriptase reaction (Roche Diagnostics).
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bioRxiv - Immunology 2020Quote: ... Tagmented DNA was ligated to unique i5/i7 barcoded primer combinations using the Illumina Nextera XT Index Kit v2 and KAPA HiFi HotStart ReadyMix (2X; KAPA Biosystems) and then purified using AmPure Beads XP (Agencourt) ...
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bioRxiv - Cell Biology 2020Quote: ... Libraries were prepared by the Genome Technologies core facility at The Jackson Laboratory using the Ovation RNA-seq System V2 (NuGEN Technologies) and Hyper Prep Kit (Kapa Biosystems). Libraries were checked for quality and concentration using the D5000 ScreenTape assay (Agilent Technologies ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Bioengineering 2021Quote: ... 1μg of total RNAs were used to prepared ribo-minus RNA-seq libraries using the KAPA Stranded RNA-Seq Kit with RiboErase (Roche, KK8484), and the depletion of rRNAs confirmed by fluorometric quantification using the Qubit RNA HS Assay kit (Invitrogen) ...