Labshake search
Citations for Roche :
4951 - 5000 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Final libraries were checked for quality and quantity using the Agilent TapeStation system and qPCR using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche). Sequencing was conducted in the Illumina NovaSeq platform (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... 1 µg of purified RNA per sample was used to generate sequencing libraries following the manufacturer’s protocols of KAPA mRNA HyperPrep Kit (KK8581, Roche Life Science). The libraries were indexed using KAPA Unique Dual-Indexed Adapter Kit at 7 µM (KK8727 ...
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bioRxiv - Microbiology 2022Quote: ... and the concentration of the differently indexed samples determined using a KAPA Library Quantification Complete kit (Kapa Biosystems, Woburn, MA, USA). Sequencing was performed using the MiSeq Reagent Kit v3 (600 cycle) ...
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bioRxiv - Biochemistry 2022Quote: ... This native whole cell clone was further used as a template to make the G148P mutant (MopRG148PLUC) in mopR - Pm/Pmop by employing standard ‘site-directed mutagenesis’ protocol using the “site-directed mutagenesis kit” from Kapa biosystems. The cloned MopRG148PLUC construct was then transformed into Escherichia coli DH5α cells and pre-inoculum was setup at 37°C overnight with the transformed colonies for performing the luciferase assay.
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bioRxiv - Evolutionary Biology 2022Quote: ... Each ligated DNA was quantified by qPCR using the Kapa Library quantification kit (Kapabiosystems) on a LightCycler 480 (Roche Life Science) prior to pooling (4 ...
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bioRxiv - Cell Biology 2022Quote: ... Medium calcium content was measured by photometrical absorption measurement of calcium EDTA complex using a Calcium Gen.2 kit (Roche, Germany). After 7 days ...
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bioRxiv - Cancer Biology 2022Quote: ... expanded to passage 4 and transfected with RCAS-PDGFB-HA or RCAS-shp53-RFP using a Fugene 6 Transfection kit (Roche, 11814443001). Cells were cultured with DMEM media (Gibco ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 600 ng of purified gDNA was used to construct a sequencing library using KAPA HyperPlus library preparation kit (Roche Diagnostics). Final library QC for size distribution verification was done on Caliper Lab Chip GX (Perkin Elmer ...
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bioRxiv - Microbiology 2021Quote: ... The final concentration of the DNA library was quantified with real time PCR using the Kapa library quantification kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... as per the manufacturers protocol. Libraries were quantified using Qubit (Life Tech Qubit high sensitivity DNA kit Cat. #Q32854) and Kapa (Kapa Biosystems, KAPA Library Quantification Kit ...
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bioRxiv - Neuroscience 2020Quote: ... and the final concentration was estimated by qPCR using primers shown in Supplementary Table S5 and KAPA Library Quantification Kit Illumina Platforms KK4828 (Roche #07960166001) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit (Kapa Biosystems, Woburn, Massachusetts) prior to cluster generation ...
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bioRxiv - Molecular Biology 2020Quote: ... Poly-A enriched libraries were generated using the Illumina TruSeq Standard mRNA preparation kit and checked via the Bioanalyzer and quantitative PCR (KAPA Biosystems). Paired-end reads (100 nt ...
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bioRxiv - Neuroscience 2021Quote: Cytotoxicity in primary and cell line cultures was assessed using the cytotoxicity Lactate DeHydrogenase (LDH) detection kit according to the manufacturer’s instructions (Roche, Basel, Switzerland). The culture medium was centrifuged at 500xg for 5min to pellet cell debris before used in the experiments.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Indexed libraries (that met appropriate cut-offs for both) were quantified by qRT-PCR using a commercially-available kit (KAPA Biosystems), and insert size distribution was determined using a LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... the PCR products digested with indicated restriction enzymes and ligated into the pBAD28 vector using the Rapid DNA Ligation Kit (Roche Diagnostics). Inserted DNA sequences were confirmed by DNA sequencing (StarSeq).
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bioRxiv - Cell Biology 2021Quote: ... cells transfected with a combination of RCAS plasmids (RCAS PDGFB-HA, RCAS shp53-RFP) using the FuGENE 6 transfection kit (Roche, 11814443001) according to the manufacturer’s protocol and as previously described 86 ...
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bioRxiv - Genomics 2020Quote: ... The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA-Illumina library creation kit (KAPA biosystems). The prepared library was then quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Cell Biology 2021Quote: ... the small fragments from the cDNA amplification are purified and the library is barcoded and amplified using PCR with the KAPA high fidelity PCR kit(KAPA Biosystems). After all libraries are ready their quality and size are measured using the bioanalyzer before being sequenced on a NEXTseq (Illumina).
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bioRxiv - Cell Biology 2022Quote: ... Gene expression was analyzed by LightCycler 480 SYBR Green Ⅰ Master kit with LightCycler 480 Detection System (Roche Diagnostics, Mannheim, Germany). qPCR values were normalized by GAPDH expression ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The fragments were treated with end-repair, A-tailing, and ligation of Illumina compatible adapters (IDT, Inc) using the KAPA-Illumina library creation kit (KAPA biosystems). Additionally ...
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bioRxiv - Genetics 2022Quote: ... Libraries are validated on a Bioanalyzer DNA High Sensitivity chip to check for size and absence of primer dimers and quantified by qPCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were paired-end sequenced on an Illumina HiSeq X platform following Illumina’s recommended protocol to generate paired-end reads of 150-bases in length.
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bioRxiv - Genetics 2022Quote: ... Hybridization was performed as described in the Instruction Manual of the DIG High Prime DNA Labeling and Detection Starter Kit I (Roche Diagnostics). Washing conditions was performed according to Huang et al (Huang et al ...
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bioRxiv - Developmental Biology 2022Quote: ... 100ng of total RNA were then sent to the Harvard University Bauer Core Facility for library preparation with the Kapa mRNA Library Prep kit (Roche, Basel). Generated RNA-seq libraries were then sequenced on a single sequencing lane of the Illumina NextSeq 500 using 2×38bp reads at the Harvard University Bauer Core Facility – see Table S1 for per-sample sequencing information ...
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bioRxiv - Cancer Biology 2022Quote: ... The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems). Cluster generation was performed according to the manufacturer’s recommendations for onboard clustering (Illumina) ...
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bioRxiv - Developmental Biology 2020Quote: ... Strand-specific polyA-enriched libraries were prepared using the Kapa mRNA-seq Hyper kit (Kapa Biosystems, Cape Town, South Africa KK8581). Untreated samples were sequenced on an Illumina HiSeq4000 with 1×100bp reads ...
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bioRxiv - Systems Biology 2020Quote: ... We generated digoxigenin (DIG)-labeled mRNA antisense probes against Nrxn3 using reverse-transcribed mouse cDNA as a template and an RNA DIG-labeling kit from Roche (Sigma). Primer and probe sequences for the Nrxn3 probe is available in Allen Brain Atlas (http://www.brain-map.org) ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Enrichment success and size-adjusted DNA concentrations of pools were assessed using the SYBR FAST qPCR kit (Kapa Biosystems, Wilmington, MA) and all pools were combined into an equimolar final pool ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... The reagents were added in the dark according to the instructions of the In Situ Cell Death Detection Kit (Roche, US). Images were acquired under a fluorescence microscope.
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bioRxiv - Pathology 2020Quote: ... Libraries were measured using Agilent 2200 TapeStation System and quantified using the KAPA Library Quant Kit ABI Prism qPCR Mix (Roche KK4835). Libraries were sequenced on a NextSeq 500 at SE75 and the reads mapped to the genome using RRBSMAP and PCR duplicates removed by proprietary script ...
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bioRxiv - Microbiology 2020Quote: ... and cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit for Illumina library quantitation (Kapa Biosystems) prior to cluster generation ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of each library was determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce appropriate cluster counts Illumina NovaSeq6000 instrument ...
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bioRxiv - Pathology 2020Quote: ... cDNA was synthesized using 500 μg of total RNA and reagents from the Transcriptor First Strand cDNA Synthesis kit (Roche Diagnostics), using the following thermal profile ...
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bioRxiv - Microbiology 2021Quote: ... were used for the purification steps and the libraries were amplified (9 amplification cycles) with the KAPA HiFi PCR Kit (Kapa Biosystems) in KAPA HiFi Fidelity Buffer (5x) ...
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bioRxiv - Microbiology 2021Quote: The full-length hAce2 gene was PCR-amplified from Mammalian Gene Collection cDNA clone (clone number MGC:47598) using a Kapa HiFi PCR kit (Kapa Biosystems) with a primer pair (Forward ...
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bioRxiv - Microbiology 2021Quote: ... the SARS-CoV-2 Spike gene with a 54-nucleotide deletion at the C-terminus was PCR-amplified from synthetic DNA (provided by Alex Ma, Academia Sinica, Taiwan) using the Kapa HiFi PCR kit (Kapa Biosystems) with a primer pair (Forward ...
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bioRxiv - Microbiology 2021Quote: ... Ten nanograms of cDNA were used as a template in a 5- l reaction mixture from a KAPA SYBR FAST qPCR kit (Kapa Biosystems). Primers used are listed in table S3 ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from well-developed leaves of 14-day plants according to the modified CTAB procedure (Murray and Thompson, 1980) or by using the KAPA3G Plant PCR Kit (Kapa Biosystems). The PCR for genomic DNA isolated by CTAB was carried out in a 25 ml reaction mixture using Platinum Taq DNA Polymerase (Invitrogen ...
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bioRxiv - Genetics 2020Quote: ... cDNA was synthesised from the total RNA with anchored-oligo(dT)18 primer and random hexamer primer using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany). One μg of RNA was added to each 20 μl RT reaction ...
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bioRxiv - Developmental Biology 2020Quote: PCR was performed on genomic DNA extracted from cell pellets or mouse tails to determine the loss of the Y chromosomal genes Uba1y and Ssty1 by utilizing KAPA Mouse Genotyping Kit (Roche, KK7302). Specific procedures were followed according to the reagent instructions ...
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bioRxiv - Genomics 2021Quote: ... The Illumina sequencing libraries were prepared by the Genomic Technology Core (Institute of Plant and Microbial Biology, Academia Sinica) using KAPA LTP Library Preparation Kit (KK8232; Roche, Switzerland) and KAPA Dual-Indexed Adapter Kit (KK8722 ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems). Library quality was assessed by BioAnalyzer (Agilent ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... a paired-end library with a target insert size of 550-bp was prepared using KAPA LTP Library Preparation Kit (KK8232; Roche, Switzerland) without amplification ...
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bioRxiv - Genetics 2020Quote: ... Amplicons were pooled to 10 nM in EB buffer and the final concentration was determined using the Illumina Universal qPCR Amplification kit from Kapa Biosystems. All pooled samples were diluted to 4 nM ...
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bioRxiv - Genomics 2020Quote: ... Libraries were assessed using Bioanalyzer DNA High Sensitivity chips and quantified by quantitative PCR using Kapa Library Quantification Illumina/ABI Prism Kit protocol (KAPA Biosystems). Validated libraries were pooled in equimolar quantities and paired-end sequenced on an Illumina HiSeq X platform following Illumina’s recommended protocol to generate paired-end reads of 150 bases in length.
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bioRxiv - Evolutionary Biology 2021Quote: ... The fragments were end-repaired, A-tailed and ligated to Illumina compatible adapters (IDT, Inc) using a KAPA-Illumina library creation kit (KAPA biosystems). For CLR libraries ...
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bioRxiv - Cell Biology 2020Quote: ... Library size distribution was checked on an Agilent high-sensitivity DNA chip and the concentration of the indexed library was determined using the KAPA library quantification kit (KAPA Biosystems) according to the manufacturer’s instructions on the StepOnePlus system (Applied Biosystems) ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA libraries were quantitated using qPCR in a Roche LightCycler 480 with the Kapa Biosystems kit (Kapa Biosystems, Woburn, Massachusetts) prior to cluster generation ...
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bioRxiv - Genomics 2021Quote: ... RNA sequencing libraries were prepared on a Beckman Coulter Biomek i7 liquid handling platform using Roche Kapa mRNA HyperPrep strand specific sample preparation kits (Roche 08098123702) from 200 ng of purified total RNA according to the manufacturer’s protocol ...