Labshake search
Citations for Roche :
4701 - 4750 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... Between 50 and 100 ng of RNA was used as input for the KAPA RNA HyperPrep Kit with RiboErase (Human/Mouse/Rat) library preparation (Roche) on an automated liquid handling platform (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2022Quote: ... In all available cases staining was performed on the Benchmark ultra-automated Stainer (Ventana) using diamino-benzidine as chromogen (DAB detection kit; Ventana, Roche), and anti-AGR2 monoclonal antibody (1:100 ...
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bioRxiv - Genomics 2022Quote: ... Pooled library (18 total – nine from DNA and 9 from total RNA – see below) concentration was determined using a KAPA library quantification kit (Roche). Libraries were sequenced on a Illumina NextSeq 500 instrument (2×150bp paired-end reads ...
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bioRxiv - Microbiology 2022Quote: ... [30] The RNA was then extracted with the MagNA Pure 96 DNA and Viral NA Small volume kit (06 543 588 001, Roche) on the MagNA Pure 96 Instrument (06541089001 ...
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bioRxiv - Cancer Biology 2022Quote: ... 2-10 5 micron sections were extracted using the High Pure FFPE RNA Isolation kit (Roche Life Sciences, Penzberg, Germany) under RNase free conditions following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting RNA was used as input into library preparation at the first strand synthesis step using the KAPA RNA Hyper Prep Kit (Roche). Libraries were sequenced to a depth of 20 million paired-end 2×150bp reads each on the NovaSeq 6000 (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and visualized with anti-DIG-AP antibody according to the DIG High Prime DNA Labeling and Detection Starter Kit II (Roche) instructions on a Chemidoc MP.
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bioRxiv - Cancer Biology 2022Quote: ... followed by DNA purification and amplification for the library with index primers using KAPA HiFi HotStart Kit (Roche, Indianapolis, IN). The libraries were purified ...
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bioRxiv - Cell Biology 2022Quote: ... RNA-Seq library was prepared according to KAPA mRNA HyperPrep kit with 200-300 bp insert size (Roche, Wilmington, MA) using 250 ng of total RNAs as input ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was performed using the Luna Universal One-Step RT-qPCR Kit (Biolaps) according to the manufacturer protocol on a LightCycler real-time PCR thermocycler (Roche). The analysis of the infection rate based on PCR data was done with the binGroup package [42] in R software.
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bioRxiv - Microbiology 2024Quote: ... Plate-based DNA library preparation for Illumina sequencing was performed on the PerkinElmer Sciclone NGS robotic liquid handling system using Kapa Biosystems library preparation kit (Roche). 200 ng of sample DNA was sheared to 300 bp or 600 bp using a Covaris LE220 focused-ultrasonicator ...
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bioRxiv - Cancer Biology 2024Quote: ... Fluorophores with the least autofluorescence on FFPE tissue were selected to minimize false positives: Cyanine 5 (Cy5) (DISCOVERY CY5 Kit, Cat#760238, Roche Diagnostics Corporation ...
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bioRxiv - Cancer Biology 2024Quote: The Terminal dUTP Nick End Labeling (TUNEL) and In Situ Cell Death Detection Kits (TMR Red #12156792910; Roche Applied Science) were used to examine apoptosis ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA (1 μg) was reverse transcribed to cDNA using the Transcriptor First Strand cDNA Synthesis Kit (catalog no. 04379012001, Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by primary antibody labelling (16-120 min) and signal detection carried out with the OptiView DAB IHC Detection Kit (VENTANA/Roche), as described previously (33) ...
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bioRxiv - Cell Biology 2024Quote: ... A total of 500 ng of RNA were then retrotranscribed using the Transcriptor First Strand cDNA Synthesis kit (Roche LifeScience) as previously described (Montañés et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA-seq libraries were prepared using 500 ng of total RNA by using KAPA stranded RNA-seq kit for Illumina (Roche) as per manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2024Quote: ... To detect for DNA containing complementary sequences on membrane-bound DNA a α-32P-dCTP-labelled probe spanning the region of 37-611 nts on human mtDNA was synthesized using High Prime DNA Labeling Kit (Roche). After pre-hybridizing the membrane with Church’s buffer (250 mM NaPi pH 7.2 ...
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bioRxiv - Microbiology 2024Quote: ... and 100 ng were used for paired-end Illumina sequencing library preparation using the KAPA HyperPlus Library Preparation Kit (Roche) and the KAPA Unique Dual-Indexed Adapater Kit (Roche) ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic PCR for ATP13A2 floxed or KO alleles was performed using 100 ng genomic DNA and the Kapa2g Fast HotStart PCR Kit (Roche). PCR primers included a forward primer (5’-CTGCAGCTTCGAGAGGAAAG-3’) ...
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bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
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bioRxiv - Molecular Biology 2024Quote: ... A first PCR reaction was prepared from genomic DNA using the KAPA HiFi HotStart PCR Kit (KAPA Biosystems/Roche Sequencing) in a total reaction volume of 20 µL ...
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bioRxiv - Neuroscience 2024Quote: ... RNAseq libraries were prepared by KAPA Stranded mRNA Sample Preparation Kit according to the manufacturer’s protocol (Kapa Biosystems, MA, USA) as previously described (Grabowska et al. ...
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bioRxiv - Microbiology 2024Quote: ... genomic DNA was obtained by automated purification using the MagNA Pure 96 DNA and Viral NA Small Volume Kit and DNA Blood ds SV 2.0 protocol (Roche Diagnostics). The DNA was quantified with Qubit fluorometer (Invitrogen ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The short insert paired-end libraries for the whole genome sequencing were prepared with PCR free protocol using KAPA HyperPrep kit (Roche). The library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and library preparation was carried out using the Accel-NGS 2S Plus DNA Library Kit (Swift Bioscience) and amplified with the KAPA HiFi DNA polymerase (Roche). The amplified library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Genetics 2024Quote: ... In order to link the sequencing adaptors and double indexed libraries the second PCR step were performed using TG Nextera® XT Index Kit (FC-131-200(1,2,3) and the Kappa Hifi Hot Start (Roche KK2602) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... One hundred ng of genomic DNA was used for PCR amplification of the target loci using KAPA HiFi HotStart PCR kit (Roche) and the primer sets in Table S1 ...
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bioRxiv - Genetics 2024Quote: ... Libraries were quantified by real-time PCR using the KAPA Library Quantification Kit for Illumina Sequencing Platforms (Roche, Basel, Switzerland), followed by quality control using a DNA 100 Chip on a Bioanalyzer 2100 ...
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bioRxiv - Neuroscience 2023Quote: ... RNA-seq libraries were prepared by the Van Andel Genomics Core from total RNA using the KAPA RNA HyperPrep Kit with RiboseErase (v1.16) (Kapa Biosystems). Base calling was done by Illumina NextSeq Control Software (NCS ...
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bioRxiv - Neuroscience 2023Quote: ... RNA sequencing libraries were prepared with 200 ng of total RNA using KAPA RiboErase Stranded RNA-Seq kit (cat # KK8483) following standard protocol (Roche). Sequencing was done at Stanford Functional Genomics Facility (Stanford ...
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bioRxiv - Neuroscience 2023Quote: ... or Ultra View Universal Alkaline Phosphatase Red Detection Kit (yielding pink signals) using standard machine settings (all solutions were from Ventana, Roche). Counterstaining (light blue background ...
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bioRxiv - Systems Biology 2023Quote: ... the entire eluate was used as input for a 300 μl PCR reaction mastermix with the KAPA HiFi HotStart DNA Polymerase kit (Roche Sequencing Solutions Inc ...
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bioRxiv - Microbiology 2023Quote: ... Probes were obtained by PCR using the primers pGK-S55 and pGK-S53 (Table S1) and labelled with the PCR DIG Probe Synthesis Kit (Roche) according to the manufacturer and revealed using the DIG Luminescent Detection Kit and DIG Easy Hyb (Roche).
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bioRxiv - Molecular Biology 2023Quote: ... the cDNA was used for real-time quantitative PCR (qPCR) with the Kapa HiFi HotStart Library Amp kit (Roche, KK2612). For Illumina short-read sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... Purified libraries were controlled by capillary electrophoresis (LabChip - Perkin) and quantitated by qPCR (KAPA Library Quantification Kits Illumina Platforms - Roche). Pooled libraries (12 pM ...
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bioRxiv - Genomics 2023Quote: High quality short-read libraries were also generated and sequenced from the same DNA samples using a Kapa HyperPrep kit (Roche) and a DNBseq G50 platform by BGI Genomics (Hong Kong ...
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bioRxiv - Molecular Biology 2023Quote: cDNA libraries were generated from the RNA samples using the Kapa RNA HyperPrep Kit with RiboErase (Roche, Indianapolis, IN, USA). Quality was measured by Agilent D1000 Screen Tape (Santa Clara ...
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from mouse tail tip or ear notch biopsies using the High Pure PCR Template Preparation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Library preparation was performed using the Kapa Hyper Prep library preparation reagents following the manufacturer’s protocol (Kapa Hyper Prep Kit, Roche 07962363001). ChIP libraries were sequenced using the Illumina HiSeq 4000 at 50 bp single reads or NovaSeq 6000 at 100 bp single reads.
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bioRxiv - Bioengineering 2023Quote: ... RNAs were then converted into individually barcoded polyadenylated mRNA sequencing libraries using the Kapa Hyper Stranded mRNA library kit (Roche) and fused with unique dual indexes ...
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bioRxiv - Cancer Biology 2023Quote: ... or OSU-ERb-12 (100nM) + Enza (1uM) for 48h and processed as previously(24) reported using Annexin-V-FLUOS staining kit (Roche) and Propidium Iodide ...
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bioRxiv - Cell Biology 2023Quote: ... First-strand cDNAs were obtained by reverse transcription of 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... The Van Andel Genomics Core prepared RNA libraries from 500ng of total RNA using a KAPA mRNA Hyperprep kit (v4.17, Kapa Biosystems). RNA was sheared to 300-400bp ...
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bioRxiv - Neuroscience 2023Quote: ... A dioxigenin (DIG)-11-UTP-labeled antisense riboprobe targeting the gene transcript of interest was synthesized using the DIG labelling kit (Roche) and T7 RNA polymerase80 ...
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bioRxiv - Neuroscience 2023Quote: ... The concentration of each library was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce cluster counts appropriate for the Illumina NovaSeq6000 instrument ...
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bioRxiv - Systems Biology 2023Quote: ... 2 ng of the re-cloned library were used as template and a 367 bp barcode-variant fragment was amplified using the KAPA HiFi HotStart DNA Polymerase Kit (Roche Sequencing Solutions Inc ...
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bioRxiv - Plant Biology 2023Quote: Quantitative real-time PCR (qRT-PCR) analysis was performed using the KAPA SYBR FAST qPCR kit (KAPA Biosystems Inc., Switzerland) to check the gene expression levels of GLXI;1 ...
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bioRxiv - Immunology 2023Quote: ... The equally mixed pool of VH and Vκ/Vλ gene fragments was subjected to overlap extension PCR to generate scFv genes using a KAPA Biosystems kit (Roche) with overlap extension primers (No ...
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bioRxiv - Plant Biology 2023Quote: ... Paromomycin probe was labeled with dUTP-DIG during PCR reaction (PCR DIG probe synthesis kit, Roche; see table primers list). Membrane was pre-hybridized 1h at 50°C in a DIG eazy Hyb (Roche ...