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Citations for Roche :
4751 - 4800 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Clear supernatant was incubated with 2 ml of pre-equilibrated Ni-beads (cOmplete™ His-Tag Purification Resin, Roche, Switzerland) for 1 h and then transferred to a sigma column to be washed consecutively with His-binding buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... 0.1 mg/mL lysozyme (∼20,000 U/mg; Carl Roth) and one tablet cOmplete™ ULTRA EDTA-free protease inhibitor tablet (Roche). Cells were lysed by homogenization using an EmulsiFlexC5 (Avestin Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... the water was removed with a pipette and 75 μl of Luminol-Mastermix (2 μg/ml horseradish peroxidase (Type II, Roche), 5μM L-012 (WAKO chemicals) ...
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bioRxiv - Neuroscience 2020Quote: ... MSNs were harvested in M-PER Mammalian Protein Extraction Reagent (Pierce) with cOmplete Mini EDTA-free protease inhibitor (1 tablet/10 mL) (Roche). Whole-cell lysates were sonicated with a 5 second pulse followed by a 5 second rest (X 5 times ...
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bioRxiv - Plant Biology 2021Quote: ... Elicitation was performed with the indicated concentration of peptides and 2 μg/ml horseradish peroxidase (Type II, Roche, Penzberg, Germany) and 5μM L-012 (FUJIFILM Wako chemicals ...
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bioRxiv - Microbiology 2021Quote: ... the remaining dermis was washed in Ca2+ and Mg2+ free PBS 5 times and incubated in a digestion buffer containing 2 mg/ml collagenase A (Roche), 100 µg/ml of DNase I (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 60 μl of anti-mouse magnetic beads were washed PBS/BSA (5 mg/ml BSA) and incubated with 3 μl of anti-Myc (Roche) or Anti-Rpb3 antibodies (Neoclone ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting powder was resuspended in 50 ml TAP Lysis buffer plus 1 mM PMSF and 1X Complete EDTA-free protease inhibitor cocktail (Roche). The lysate was cleared by centrifugation at 4000 g for 10 min and then incubated with 1 ml (packed volume 600 μl ...
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bioRxiv - Microbiology 2021Quote: ... The column was washed with 200 mL of the equilibration buffer supplemented with 1 mM PMSF and EDTA-free protease inhibitor (Roche). SolPacL1 and SolPacL1Δ3 were then eluted with 25 mL of the equilibration buffer containing 1 mM PMSF and 250 mM imidazole.
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bioRxiv - Cancer Biology 2022Quote: ... The eluted chromatin was then treated with RNaseA (10mg/mL) for 1 hour at 37°C and with Proteinase K (Roche) for 2 hours at 55°C ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting fat pads were rinsed in PBS and minced in ice-cold digestion buffer (7.5 mg/ml Collagenase D (Roche 1108886001), and 4.8 mg/ml Dispase II (Sigma D4693 ...
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bioRxiv - Molecular Biology 2022Quote: ... was run on a 15% polyacrylamide gel containing 7M urea and 10 µg/ml APM and then transferred to positively charged nylon membranes (Roche). Pre-hybridization and hybridization steps were performed with Dig Easy Hyb (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were diluted to a final total soluble protein concentration of 1 mg/ml in TBS containing cOmplete mini protease inhibitor tablet (Roche). For AD ...
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bioRxiv - Immunology 2022Quote: ... Tissues were subject to a second enzymatic digestion in RPMI 1640 medium supplemented with 25 μg/mL Liberase TM (Roche) and 0.25 μg/mL DNAse I and incubated for 30 minutes at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... The cells were washed with PBS and crosslinked for 30 min in 2 ml ice cold 1% formaldehyde containing 2X Complete EDTA-free Protease Inhibitor Cocktail (Roche). Cross-linking was stopped by the addition of glycine to 333 mM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... resuspended at 1 million cells per mL in CETSA buffer and dispensed (10 μL per well) into 384-well PCR plates (Roche) using a Multidrop Combi (ThermoFisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... and prepared for flow cytometry immunostaining as follows: Matrigel-embedded organoids were digested using a HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml of collagenase type IV (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... The clarified supernatant was filtered through a 0.45 μM cartridge filter and incubated with 10 mL of cOmplete His- Tag purification beads (Roche, Germany) for 45 min ...
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bioRxiv - Cell Biology 2022Quote: All strains expressing NUP-RITE constructs were grown to mid log phase in SCD supplemented with 300 μg/mL hygromycin B (Roche) to select for non-recombined cells ...
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bioRxiv - Immunology 2020Quote: ... Labelled cells were washed twice with 1mL FACS buffer and resuspended in 500uL FACS buffer containing DNAse I (10ug/mL, Roche). Sorting of the cells was performed into 500uL RNAlater on FACSAria III (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... One mL of yeast lysate (1-2 mg/mL) prepared in column buffer freshly supplemented with protease and DUB inhibitors (cOmplete mini EDTA-free (Roche), 10 mM NEM ...
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bioRxiv - Neuroscience 2021Quote: ... This was spun down (5 minutes at 1800 RMP, 4°C) and the pellet then digested in 10ml collagenase/dispase (1mg/ml; Roche) and DNase I type IV (40µg/ml ...
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bioRxiv - Molecular Biology 2021Quote: ... The washed pellet was resuspended in approximately 1/3 of packed cell volume of ORC lysis buffer containing cOmplete Protease Inhibitor Cocktail Tablet (1 tablet per 25 mL total volume; Roche) and frozen dropwise in liquid nitrogen.
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bioRxiv - Molecular Biology 2020Quote: ... Explants were diced and then digested by sequential incubations with pronase (70 U/mL for 1 hour at 37°C; Roche) and collagenase (0.2% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... 10 mM Tris-HCl (pH 7.4) and 1 mg/ml BSA) supplemented with 10 μl of DIG RNA labeling mix (Roche 12039672910) and incubated for 1 h at 37°C with occasional gentle tapping ...
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bioRxiv - Neuroscience 2021Quote: ... Slides were then pretreated with TdT buffer (25 mm Tris-HCl, 200 mm sodium cacodylate, 0.25 mg/ml BSA, 1 mm cobalt chloride, Roche Diagnostics) at RT for 10 min ...
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bioRxiv - Bioengineering 2021Quote: ... P14 CD8+ T cells were activated for 24 h as described above and resuspended in T cell media + 30 U/ml rhIL-2 (Roche) at 2 × 106 cell/ml ...
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bioRxiv - Bioengineering 2021Quote: ... The skin was finely minced with a scalpel and placed for 30 min at 37 °C on a shaker in a digesting enzyme cocktail of 2 mg/ml Collagenase P (Roche), 2 mg/ml Dispase (Gibco ...
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bioRxiv - Cancer Biology 2020Quote: ... samples were treated for 1 hr at 55⁰C with proteinase K solution (0.4 mg/mL proteinase K (Roche; 3115828001), 50 mM EDTA ...
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bioRxiv - Molecular Biology 2022Quote: ... IMAC lysis buffer (1.5 ml buffer per gram cell pellet) and the complete stock solution (1 tablet Complete EDTA-free (protease inhibitor cocktail, Roche) and 50 μl benzonase nuclease cell resuspension (PSF ...
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bioRxiv - Molecular Biology 2022Quote: ... Enzymatic digestion was carried out in complete DMEM/F12 medium implemented with 1.4 mg/mL Collagenase A (Roche, Penzberg, Germany), for 4–6 hours in a humidified incubator at 37°C and 5% CO2 ...
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bioRxiv - Developmental Biology 2022Quote: ... The liver was dissected from the abdominal cavity and placed into a solution containing Hanks’ buffered saline solution (HBSS) supplemented with 1.07 Wünsch units/ml Liberase DH (Roche Applied Science) and 70 U/ml hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Systems Biology 2022Quote: Limb buds were dissected and incubated for 5 min in 270 µL of 0.22 Wunsch unit/mL Liberase TM (Roche, 5401119001) in EDTA-free ...
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bioRxiv - Genomics 2022Quote: ... Pellets were harvested after 16-18h and resuspended in lysis buffer (50 mM Tris-HCl, 500 mM NaCl, 5% glycerol, 1 tablets per 50 ml protease inhibitor (EDTA-free, Roche), 0.2 mM PMSF ...
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bioRxiv - Immunology 2022Quote: ... PBMCs were incubated with an equimolar amount of DMSO as negative control and with phytohemagglutinin (5 μg/ml) (PHA, Roche) as a positive control ...
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bioRxiv - Cancer Biology 2019Quote: ... PDX tumors were minced with No.22 blades into 1-2 mm fragments then digested with 1mg/ml collagenase/dispase (Roche) for 30-40 minutes ...
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bioRxiv - Molecular Biology 2019Quote: ... for 1 h at 37°C and protein was removed by incubation with 10 μL of proteinase K (18 mg/mL; Roche) for 2 h at 65°C ...
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bioRxiv - Developmental Biology 2019Quote: ... testis lysates were prepared by sonication of ∼100-mg pieces of freshly removed whole testis in 1.0 mL ice-cold RIPA buffer containing protease inhibitors (cOmpleteTM, EDTA-free Protease Inhibitor Cocktail Tablets, Roche, 4693159001) and phosphatase inhibitors (PhosSTOPTM ...
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bioRxiv - Genetics 2019Quote: BAL was performed by cannulation of the trachea and gentle instillation/aspiration (3 times) of 1.0 ml PBS with 0.1% BSA and protease inhibitor cocktail tablets (Roche, Indianapolis, IN). Animals were then perfused with PBS before tissue collection ...
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bioRxiv - Immunology 2020Quote: ... mice were perfused with sterile PBS and the right inferior lung lobes were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: Pancreatic tumors were resected from the mice and mechanically and enzymatically digested in Hanks Balanced Salt Solution (HBSS) with 1 mg/mL Collagenase P (Roche) Cell suspensions were centrifuged for 5 min at 1500 rpm ...
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bioRxiv - Pathology 2020Quote: Lungs harvested and cut into small pieces were digested (1h, 37c) with 4 mg/ml collagenase D (Roche, Mannheim, Germany). Tissue was meshed through a 40-µm cell strainer ...
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bioRxiv - Biochemistry 2020Quote: ... His – tagged SARS-CoV2 S trimers were purified using a two-step purification protocol by 1 mL or 5mL cOmplete His-tag columns (Roche). Proteins were further purified by size-exclusion chromatography using a HiLoad Superdex 200 16/600column (GE Healthcare).
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bioRxiv - Cell Biology 2021Quote: ... 1 mM MgCl2, 10% glycerol, 1% Tween-20, 20 mM imidazole, 1 mg/ml lysozyme, and 1x protease tablet [Roche]) for thirty minutes on ice with 5 min intervals of 10 s vortexing ...
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bioRxiv - Immunology 2020Quote: ... intestines were further chopped and incubated for 30 min at 37°C in medium containing liberase (20 µg/ml, Roche) and DNAse (400 µg/ml ...
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bioRxiv - Microbiology 2021Quote: ... 1 mM NaF, 1 mM Na3VO4, 1 mM PMSF, protease inhibitor cocktail [1 tablet in 50 mL lysis buffer, Roche]). The whole cell extract was denatured ...
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bioRxiv - Microbiology 2021Quote: ... The cell pellet was resuspended in 10 ml PdeL purification buffer and one tablet of c0mplete mini EDTA-free protease inhibitor (Roche) and a spatula tip of DNase I (AppliChem ...
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bioRxiv - Zoology 2020Quote: ... the digoxygenin probe is visualized by replacing the APB is replaced with the same containing 20 µl/ml of a commercial NBT/BCIP stock solution (Roche Diagnostics GmBH ...
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bioRxiv - Physiology 2020Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... the parasites were re-suspended in 1xPBS containing 1 mg/ml soybean trypsin inhibitor and 25x protease inhibitor cocktail (Roche) before the parasites were pelleted and lysed in lysis buffer (4% SDS ...