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Citations for Roche :
5001 - 5050 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were maintained in growing media (DMEM + Glutamax, 10% FBS, pen/strep 5ml) supplemented with 0.8mg/ml G418 (Roche, G418-RO) for maintenance or removed for exposure to chemicals ...
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bioRxiv - Cancer Biology 2024Quote: ... If stained with phalloidin (1/500, Scientific Laboratory Supplies P1951) and DAPI (1/1000 in PBS from 1 mg/ml stocks, Roche 10236276001), or just DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... The cell pellets were resuspended in 1 ml of homogenization buffer (250 mM sucrose in 20 mM HEPES, pH 7.4, with protease inhibitor mix “Complete”, Roche Rotkreuz, Switzerland) and then sheared by passing through a 27x gauge needle 10 times.
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bioRxiv - Cell Biology 2022Quote: ... and then the detection reaction was carried out in a buffer containing 3.5 µl/ml of of nitro blue tetrazolium (NBT) and 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Roche, Bâle, Switzerland). The reaction was stopped with 50 mM EDTA in PBS and embryos were washed in PBSTw ...
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bioRxiv - Molecular Biology 2022Quote: ... and the cell pellet resuspended in 1 ml of PBS with the addition of a protease inhibitor cocktail (cOmplete™ Protease Inhibitor Cocktail, Roche) in order to protect histones from degradation by proteases during the next step of cell lysis ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then resuspended in 10 ml of cold PBS and incubated with cOmplete mini EDTA-free protease inhibitor (Roche, 11836170001) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... 5 mM β-mercaptoethanol and 20 μM GDP supplemented with DNase I (10 μg/ml) and cOmplete protease inhibitor cocktail tablets (Roche). Cells were mechanically disrupted using a Microfluidizer (Microfluidics International Corporation ...
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bioRxiv - Cancer Biology 2024Quote: ... The aqueous phase from each MaXtract tube (∼400 µl) was then transferred to 1.5 ml tubes containing 2 µl of 2 µg/µl glycogen (Roche, Cat# 10901393001), 1 ml ethanol was added ...
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bioRxiv - Molecular Biology 2024Quote: ... crosslinked chromatin was resuspended in 1 mL Farnham Lysis Buffer (FLB; 5 mM HEPES pH 8.0, 85 mM KCl, 0.5% NP-40/IGEPAL, Roche Protease Inhibitor Cocktail), centrifuged ...
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bioRxiv - Neuroscience 2024Quote: ... titer was determined at a concentration of 1.1013 gcp/ml by quantitative real-time PCR using the Light Cycler 480 SYBR green master mix (Roche, cat # 04887352001) with primers specific for the AAV2 ITRs (fwd 50-GGAACCCCTAGTGATG GAGTT-3’ ...
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bioRxiv - Genomics 2024Quote: The nuclei were resuspended in 250 µL of 5’ phosphorylation master mix (1X T4 PNK buffer, 500 U/mL of T4PNK, 1 mM ATP, 1 U/µL of RNAse inhibitor (Roche, 3335399001)) and incubated at 37°C while rotating at 800 rpm for 1 hour to phosphorylate the 5’ ends of RNA ...
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bioRxiv - Genetics 2024Quote: ... cut to very small pieces using a scalpel blade and digested using Collagenase D (Roche, Cat No. 11088858001, 1 mg/ml) /DNase I (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2% SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Cell lysates were centrifuged at 14000 rpm for 10 minutes at 4 0C and total protein contents were measured by Bradford protein assay ...
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bioRxiv - Cell Biology 2024Quote: ... and the adipocytes were lysed using PBS containing 2 % SDS and protease inhibitor (1 tablet per 10 mL; cOmplete Mini, Roche Diagnotics). Secretion samples were centrifuged for 5 minutes at 2000 rpm and 4 0C ...
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bioRxiv - Immunology 2024Quote: ... the cells were quickly transferred into 10 ml of pre-warmed RPMI supplemented with 10% FBS and 100µg of Pulmozyme (dornase alpha, Roche, Basel, Switzerland) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2023Quote: ... was performed by cannulation of the trachea and gentle instillation/aspiration (3 times) of 1.0 ml of PBS with protease inhibitor cocktail tablets (Roche, Indianapolis, IN). The lavage fluid was centrifuged at 4000 rpm for 5 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... CDMs were washed with PBS and treated for 1 h at 37°C with a buffer of 50 µg/ml DNaseI (Roche, #11284932001), 5 mM MgCl2 ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord samples specifically processed for the detection of intracellular MBP within phagocytic cells were predigested with 0.5 mg/mL Collagenase/Dispase® (Roche, #11097113001), 0.02 mg/mL DNase I (Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... cecal and colonic tissues were homogenized in 1 mL of PBS −/− supplemented with the cOmplete EDTA-free protease inhibitor cocktail (Roche Diagnostics). Supernatants were collected by centrifuging the homogenate at 16,000 ×g at 4 °C for 20 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
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bioRxiv - Systems Biology 2023Quote: ... per sample were washed three times with 1 ml wash buffer (20 mM HEPES at pH 7.5, 150 mM NaCl, 0.5 mM Spermidine, and 1 × Roche complete protease inhibitor) at room temperature and resuspended in 225ul of wash buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in a 0.2 ml PCR tube preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM P5 transposon primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Cancer Biology 2023Quote: ... Tissue sections were cut into small pieces (1-2 mm) and incubated with 0.25 mg/ml Liberase TL (Roche, Cat# 05401020001) at 37°C for 40 minutes ...
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bioRxiv - Microbiology 2023Quote: ... one half was immunoblotted with NB13 (7 µg/mL) followed by washing and probing with α-HA-peroxidase (1:1000, Roche), whereas the other half was probed with α-His-peroxidase only (1:4000 ...
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bioRxiv - Pathology 2023Quote: ... and kidneys were minced into small pieces and placed into 5mL digestion buffer (15mg/mL Collagenase D (Roche Applied Science, 11088858001), 10mg/mL Collagenase Type 4 (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were further incubated for 48 h and subsequently subjected to Gentamycin (G418) selection (1 μg/ml, Roche, G418-RO). After 4 days cells were sorted on a fluorescence-activated cell sorter (FACS ...
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bioRxiv - Physiology 2023Quote: ... and 1 μL of hemolymph or synthetic HA::spacer::FLAG peptide standard (DYKDDDDKGGGGSYPYDVPDYA) was diluted into 50 μL PBST + 25 ng/mL mouse anti-HA peroxidase (Roche, #12013819001) + 1% non-fat dry milk ...
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bioRxiv - Genetics 2023Quote: ... 25-30 million cells were resuspended in 1 mL lysis buffer (1% SDS, 50 mM Tris-HCl pH 8, 20 mM EDTA, 1x cOmplete (Roche, 4693132001) protease inhibitor) ...
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bioRxiv - Immunology 2023Quote: ... Total uteri and draining lymph nodes from mice perfused with PBS before organ collection were digested with DNAseI 200 units/mL (Roche 10104159001) and Liberase DL 0.3 Wunits/mL (Roche 05466202001 ...
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bioRxiv - Immunology 2023Quote: ... coated with 2.5ug/mL aCD3 and re-stimulated for an additional 3 days in complete IMDM-10 supplemented with 50U/mL IL-2 (Roche 11011456001). For Th17 polarizations ...
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bioRxiv - Biophysics 2023Quote: ... Cells were pelleted and resuspended in 50 mM Tris (pH 7) supplemented with 500 μg/mL lysozyme and 1x cOmplete EDTA-free protease inhibitor cocktail (Roche, 11873580001). The pellet was incubated at 4 ºC while rotating end over end for 45 min.
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bioRxiv - Immunology 2023Quote: ... then subject to gentle cold digestion on ice using 10% FBS-supplemented RPMI media containing 100mg/mL trypsin inhibitor from soybean (Roche, 10109886001), 10mg/mL collagenase A from clostridium histolyticum (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... 4 mM sodium dithionite) supplemented with 0.2 mg/mL bovine pancreatic deoxyribonuclease I and one cOmplete EDTA-free protease inhibitor tablet (Roche, Basel, Switzerland). Subsequently ...
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bioRxiv - Cancer Biology 2023Quote: ... The gel plugs were incubated at 50°C for 3 days and treated with fresh proteinase K at 20 mg/ml concentration (Roche Diagnostics), every 24 h ...
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bioRxiv - Plant Biology 2023Quote: ... the aerial parts were dipped for 30 s in 1 ml of 0.01% (v/v) Tween-20 aqueous solution containing 50 µM MG132 (Roche Sigma-Aldrich) in DMSO ...
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bioRxiv - Cell Biology 2023Quote: ... Crypts were isolated from the distal half of the small intestine as described above and dissociated in 10% FBS in 1X PBS supplemented with 20 μg/mL Liberase TH (Roche 05401135001), and 35 μg/ml DNaseI (Roche 10104159001 ...
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bioRxiv - Bioengineering 2023Quote: ... the membrane of day 28 ALI cultures was removed from the transwell using a scalpel and incubated in Accutase™ supplemented with 200 μg/mL DNase I (Roche) for 15-20 min in a shaking 37°C water bath ...
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bioRxiv - Physiology 2023Quote: ... Then the heart was perfused with the same solution containing 1 mg/mL collagenase Type A (Catalog # 9036-06-0; Roche, Germany) and 0.08 mg/mL protease Type XIV (Cat log # 48655321 ...
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bioRxiv - Physiology 2024Quote: ... For single cell preparation samples were incubated for 60-min at 37°C with gentle shaking in RPMI media supplemented with 50 mg/ml of Liberase TL (Roche #5401020001), 40 U/ml of DNAse I (Sigma-Aldrich #D4527) ...
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bioRxiv - Microbiology 2023Quote: ... Bacterial lysis and protein digestion were performed simultaneously by adding 50 µL of 1 mg/mL trypsin (Roche Diagnostics, Mannheim, Germany) prepared in a 150 mM NH4HCO3 solution ...
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bioRxiv - Microbiology 2023Quote: ... mycelia were resuspended in lysis buffer (10 mM Tris-HCl pH 8.0, 50 mM NaCl, 15 mg/mL lysozyme, 1× protease inhibitor (Roche, Bavaria, Germany)) and incubated at 37°C for 20 min ...
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bioRxiv - Biochemistry 2023Quote: ... The S100 extract was eluted from DEAE resin with 5 ml of S100 Elution Buffer (250 mM KHPO4 (pH 6.5),1x Mini protease inhibitor cocktail tablet (Roche, Basel, Switzerland)) and aliquoted and flash-frozen in liquid nitrogen ...
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bioRxiv - Biochemistry 2023Quote: ... and resuspended in 20 ml of S100 Lysis Buffer (10 mM KHPO4 pH 7.5, 1x Mini protease inhibitor cocktail tablet (Roche, Basel, Switzerland)) for 1 hour ...
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bioRxiv - Developmental Biology 2023Quote: ... and weighed on a fine balance to put 0.6g of skin per 3.5ml of digestion medium (0.385mg/ml Liberase TL-Roche 246749-in HBSS/PBS). Samples were incubated at 37°C for 40-60 minutes while shaking ...
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bioRxiv - Immunology 2023Quote: ... Uteri were isolated and carefully dissected to remove the yolk sac containing the fetus followed by mechanical disruption and digestion with DNase I 200 units/mL (Roche 10104159001) and Liberase DL 0.3 W units/mL (Roche 05466202001 ...
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bioRxiv - Cell Biology 2023Quote: Between 20,000 and 40,000 cells were seeded the day before the imaging experiment on 8-well ibidi dishes coated with 25 μg/ml laminin (Roche, 11243217001). When indicated ...
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bioRxiv - Plant Biology 2024Quote: ... The transformation mixture was plated on TAP medium containing 18 μg/ml Hygromycin B (Roche 10 843 555 001; Mannheim, Germany) using 0.9% agar to screen for colony morphology ...
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bioRxiv - Developmental Biology 2024Quote: Immune cell FACS was performed from ear skin from which cells were isolated using DNAse I 40 µg/ml (Roche, 11284932001) and 20 U/ml Collagenase Type I Worthington (LS0004194 ...