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Citations for Roche :
4651 - 4700 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... and the right inferior lung lobes were digested in 50 μL of 5 mg/mL of Liberase Tm (Roche) and 12.5 μL of 10 mg/mL of DNase I (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... cells were released by incubating gels in a cell culture incubator in a protease solution of 2.5 mg/mL collagenase D (Roche) in PBS until gels were fully degraded ...
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bioRxiv - Systems Biology 2023Quote: ... Up to 5 ng plasma cfDNA (~0.5 mL plasma) was used for cfWGS library with Kapa HiFi Hotstart ReadyMix (Roche) in 11-13 cycles ...
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bioRxiv - Molecular Biology 2023Quote: ... Mouse atria were minced and digested with mild agitation for 30 minutes at 37°C in DMEM containing 1% P/S and 0.14 Wunsch U ml-1 Liberase (5401119001, Roche). MAFs were enriched via negative selection with magnetic beads against mouse CD45 (leukocytes) ...
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bioRxiv - Biophysics 2023Quote: ... Applying voltage to the piezo element produced standing planar acoustic waves generating force applied to the polystyrene microspheres inside the chamber in the direction towards the top side of the chamber The AFS chip was first passivated by treatment with 0.05 mg/ml of anti-digoxigenin antibodies (Roche), followed by incubation with 0.2% BSA (Amresco ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were first mechanically dissociated with a scalpel into approximately 1-3 mm pieces and enzymatically digested in RPMI-1640 medium (Cytiva) containing 0.25 mg/ml DNase I (Roche) and 0.25 mg/ml Collagenase (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... the livers were cut and separated with forceps and digested in 3mg/mL Liberase (589994, Roche ® Life Science) and 3mg/mL DNase I (Roche ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5 mM dithiothreitol (DTT)) containing 10 µg/ml phenylmethyl-sulfonyl fluoride (PMSF) and Complete EDTA-free protease cocktail (Roche). Cells were incubated on ice for 10 min and lysed by passing five times through a 27-Gauge needle ...
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bioRxiv - Neuroscience 2023Quote: ... Tissues were washed in HBSS and then dissociated with 1mg/ml of collagenase and dispase (Roche, Indianapolis, IN, USA) for 45 mins at 37 degrees ...
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bioRxiv - Developmental Biology 2023Quote: ... dissected tissues were dissociated for 30 mins at 37 °C in 200ul of LiberaseTM (Roche, 100ug/ml in PBS). 2 mL of cold PBS (without ca/Mg ...
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bioRxiv - Genetics 2023Quote: ... incubated the islets for 10 min at 37°C in 2 mL PBS with 4U Dispase I (Roche Diagnostics) / 2U DNase I (ThermoFisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen pellets were thawed on ice and lysed with 20mls buffer per gram of pellet (50mM HEPES, 500mM NaCl, 0.5mM TCEP, 1U/ml DNase supplemented with Roche cOmplete™ ...
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bioRxiv - Biochemistry 2023Quote: ... prior to resuspension in 25 mL ice-cold water with another tablet of cOmplete™ ULTRA protease inhibitor (Roche), followed by centrifugation for 30 minutes at 15000×g and 4 °C ...
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bioRxiv - Immunology 2023Quote: ... The fixed cells were collected by scraping in 0.5 ml of ice cold 1x PBS supplemented with 1x cOmplete proteinase inhibitor (Roche) for U2OS and by centrifugation for SUDHL4 at 1000g for 5 min at 4°C ...
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bioRxiv - Genomics 2023Quote: The captured DNA was amplified for ten cycles using KAPA HiFi HotStart ReadyMix (6.25 ml, Roche, catalog number 7958935001) and purified in 1.8 x volume of Agencourt AMPure beads (Beckman Coulter ...
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bioRxiv - Developmental Biology 2023Quote: ... and thus we dechorionated Stage 24-25 embryos (or 2 to 2.5 dpf) pupfish by incubating them on 20 mg/ml Pronase (Roche) in water for 2.5 hours ...
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bioRxiv - Molecular Biology 2023Quote: Tissue disruption began physically using a pestle in 100μL of a digestion mix composed of 1mg/mL Liberase TM (5401119001, Roche), 1mg/mL Collagenase D (11088858001 ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 37°C for 1h in 0.4 mg/mL dispase II (Roche). The epidermis was collected and then cut into small pieces using scalpels and incubated for 30 minutes with agitation at 37°C in RPMI containing 10% FCS and 0.5 mg/mL DNAse I ...
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bioRxiv - Immunology 2023Quote: ... The epidermis and dermis layers were separated after incubation at 4°C for 16h in 0.2 mg/mL dispase II (Roche). Epidermal layers were fixed in 4% Para-formaldehyde for 20 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 75 μl of the sample were treated for 30 min at 50°C with 1.2 mg/ml Proteinase K (Roche) in Proteinase K Buffer (100 mM Tris-HCl ...
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bioRxiv - Developmental Biology 2023Quote: ... Minced lung was enzymatically dissociated to a single cell suspension using 1 mg/mL collagenase A (Roche, Cat# 10103578001), 2-4U/mL elastase (Worthington ...
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bioRxiv - Physiology 2023Quote: Approximately 50 mg of adipose tissue was homogenized in 1 mL of lysis buffer (250 mM sucrose, 50 mM Tris Cl, pH 7.0, with protease inhibitor cocktail (11873580001, Roche)) ...
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bioRxiv - Immunology 2023Quote: ... Brain and spinal cord were cut into pieces and digested for 30 min at 37 °C with collagenase D (3.75 mg/ml; Roche) and DNase I (1 mg/ml ...
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bioRxiv - Systems Biology 2023Quote: ... The left lobes of the lung were then inflated with digestion solution containing 1.5 mg/mL of Collagenase A (Roche) and 0.4 mg/mL DNaseI (Roche ...
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MET functions in tumour progression and therapy resistance are repressed by intronic polyadenylationbioRxiv - Molecular Biology 2023Quote: ... and reconstituted at 0.5mg/ml in water and then further diluted to a 0.1 mg/ml stock solution in PBS (gibco) 0.1% BSA (Roche, #10735086001). Agarose was purchased from Invitrogen (#16500-500).
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bioRxiv - Developmental Biology 2023Quote: ... were placed in 250µL of ice-cold disassociation media (sterilized 1x PBS and 0.04% bovine serum albumin, 1.2 U/mL Dispase II (Roche 04942078001), 1 mg/mL Collagenase IV (Gibco 17104–019) ...
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bioRxiv - Developmental Biology 2023Quote: Lungs extracted from E14.5 or E18.5 embryos were minced and digested using collagenase I and dispase I (both 1 mg/mL, Roche) and incubated at 37°C for 5 min ...
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Emergence of RNA-guided transcription factors via domestication of transposon-encoded TnpB nucleasesbioRxiv - Genetics 2023Quote: Flash-frozen cell pellets were resuspended in 1.2 ml RIP lysis buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and SUPERase•In RNase Inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed in 100μL or 200μl of 2% SDS lysis buffer (2% SDS, 50mM Tris-HCl pH 7.5, 5mM EDTA, 15U/mL DnaseI (Roche), cOmplete mini EDTA-free protease inhibitor tablet (Roche ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume). The suspension was frozen and stored at −80 °C ...
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bioRxiv - Biochemistry 2023Quote: ... then the pellet was resuspended in 40 ml binding buffer (50 mM NaPi, 300 mM NaCl, 5 mM imidazole, pH 7.5) containing protease inhibitor (Roche cOmplete EDTA-free protease inhibitor cocktail ...
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bioRxiv - Biochemistry 2023Quote: ... pH 7.5) (10 mL/g pellet weight) supplemented with DNAse and 1 cOmplete EDTA-free Protease Inhibitor Cocktail tablet (Roche) per 2 L of culture ...
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bioRxiv - Cell Biology 2024Quote: ... and the cell pellet was gently resuspended in 1 ml ice-cold vPBS containing EDTA-free protease inhibitor (Roche). The cells were again pelleted by centrifugation (1000 x g ...
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bioRxiv - Molecular Biology 2024Quote: ... at 10 mL/g of cell pellet) including protease inhibitors (complete protease inhibitor cocktail tablet, Roche Life Sciences, UK). The suspension was lysed using an Emulsiflex extruding homogenizer (Avestin ...
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bioRxiv - Biochemistry 2024Quote: ... 3 mM magnesium chloride) supplemented with 1 cOmplete protease inhibitor cocktail tablet per 50 ml of lysis buffer (Roche) and mechanically disrupted in a Dounce homogenizer ...
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bioRxiv - Bioengineering 2024Quote: ... The media was removed and 150 μL of serum free RPMI containing 0.25 mg/mL Liberase TL (Roche 054010120001) were added to the samples ...
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bioRxiv - Microbiology 2023Quote: ... the pellets were resuspended in buffer M (50 mM NaH2PO4, 50 mM Na2HPO4, pH 6.8) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche) and opened by sonication ...
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bioRxiv - Molecular Biology 2023Quote: ... flash-frozen pellets were first resuspended in 1.2 mL RIP lysis buffer supplemented with cOmplete Protease Inhibitor Cocktail (Roche) and SUPERase•In RNase Inhibitor (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2023Quote: ... thrombus samples were obtained and homogenized with 0.3 ml of PBS (pH 7.2) containing Complete Protease Inhibitor Mixture (Roche Diagnostics). The homogenates were centrifuged at 12,000 g for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol and 0.5 mM TCEP supplemented with 5 Units/mL Benzonase and an EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cell Biology 2023Quote: ... cells were detached 24 h after transfection with Trypsin-EDTA (0.25% wt/vol) and re-plated onto 10 µg/ml fibronectin (Roche) pre-coated MatTek dishes with glass bottoms ...
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bioRxiv - Molecular Biology 2023Quote: SDGC-SEC-purified stress granule cores (strain JD1370) were incubated at 0.23 A260 units/mL with 2 or 4 units of RNase H (10786357001; Roche) in 40 μL of RNase H buffer (20 mM HEPES-KOH pH 8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was stored as flow through and the beads were washed 6 times with 1 ml RIPA buffer complemented with 0.25× protease inhibitor (cOmplete, Roche). Bound proteins were eluted from agarose beads with 1 bead volume of Laemmli buffer heated at 95°C for 5 min ...
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bioRxiv - Biophysics 2024Quote: ... were resuspended in 20 mM Tris (pH 7.4) and 300 mM NaCl (50 mL volume) containing complete protease inhibitor cocktail (Roche) and lysed by soni- cation ...
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bioRxiv - Biochemistry 2023Quote: ... benzonase (to 1 µg/mL) and cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, 2 tablets/L initial culture volume) were added to the cell suspension and stirred at RT for 30 mins ...
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bioRxiv - Immunology 2023Quote: Tumors were mechanically homogenized using a Dounce Tissue Homogenizer and enzymatically digested using 0.05mg ml-1 Liberase TL (Roche), 0.2mg ml-1 Dnase I (Roche ...
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bioRxiv - Physiology 2024Quote: Islets were isolated by the Vanderbilt Islet and Pancreas Analysis (IPA) core by injecting 0.6 mg/ml collagenase P (Roche) into the pancreatic bile duct followed by a Histopaque-1077 (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... cell fusion was performed by incubating the dish with 1 ml of prewarmed polyethylene glycol 1500 (PEG 1500, Roche) for 1 min at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... spores were then washed twice with sterile PBS and resuspended in a 1.0 mg/mL Proteinase K (Roche Laboratories) in 10mM Tris-HCl (Sigma-Aldrich) ...