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Citations for Roche :
4901 - 4950 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... the common bile duct was clamped and the pancreatic duct was perfused with 3-5 mL solution of collagenase P in HBSS-1% HEPES (Roche). The pancreas was then harvested and transferred to a 50mL conical tube containing 5mL of collagenase P solution and kept on ice until all organs were collected ...
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bioRxiv - Immunology 2023Quote: ... 0.5 –1 L of the crude lysates were treated with 5 mg/mL each of DNase I and RNAse (Roche) for one hour at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... Alkaline phosphatase activity was detected in the dark in AP buffer supplemented with 45 mg/ml 4-nitrobluetetrazolium chloride (NBT, Roche) and 35 mg/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Biochemistry 2023Quote: The cell pellets were re-suspended in 2 ml of mitochondrial isolation buffer (200 mM sucrose, 10 mM Tris HCl, 1 mM EGTA/Tris, protease inhibitor cocktail (Roche), pH7.4 (adjusted with 1M HEPES)) ...
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bioRxiv - Biochemistry 2023Quote: ... The pellet was resuspended in 30 mL of 40 mM Tris pH 8 supplemented with 1 tablet of EDTA-free protease inhibitor tablet (Roche), and 0.1 mM PMSF ...
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bioRxiv - Cell Biology 2023Quote: ... the cell monolayers were scraped into 10 ml KPBS (136 mM KCl and 10 mM KH2PO4, pH 7.25, containing protease (Roche, 1836153001) and phosphatase inhibitors (PhosSTOP Sigma ...
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bioRxiv - Biochemistry 2023Quote: ... CVF samples were normalized to a protein concentration of 1 mg/mL using sterile PBS and EDTA-free protease inhibitor cocktail (Roche) was added ...
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bioRxiv - Immunology 2023Quote: ... were isolated from spleens of naïve WT C57BL/6 mice by cutting samples into small pieces and incubating at 37°C for 45 minutes with 1U/ml DNase I (Roche) and 10U/ml collagenase D (Roche ...
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bioRxiv - Immunology 2023Quote: ... Lung tissues were homogenized with a GentleMACS dissociator (Miltenyi) and digested with a cocktail of Liberase (70 μg/mL, Roche) and aminoguanindine (10mM ...
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ER mediates spatial regulation of lysosome-endosome interactions via motion switch at junction sitesbioRxiv - Cell Biology 2023Quote: ... A total of ∼1×107 HEK293T cells expressing GFP-STARD3 were lysed with 1 mL lysis buffer supplemented with a protease inhibitor cocktail (cOmplete, Roche). Lysates were clarified by centrifugation at 12000 g for 60 min and were mixed with the beads coated with 2 mg GST/GST-VAPA-MSP for 60 min at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... For Salm protein purification from bacterial lysates (lisozima 0,5mg/mL, Imidazol 10mM and protease inhibitors cOmplete™ Protease Inhibitor Cocktail tablets from Roche) we used 3 rounds of 3 minutes sonication with 15 s on/off cycles ...
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bioRxiv - Biophysics 2023Quote: ... Pellets were resuspended in 7 ml of buffer A supplemented with the addition of 200 μl of protease inhibitor cocktail (one tablet (Roche), dissolved in 1 ml buffer A) ...
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bioRxiv - Physiology 2023Quote: ... The signal was then visualized by incubating the slides for 48 hours in a solution containing 0.33 mg/mL NBT (Nitro-blue tetrazolium chloride, Roche, 11383213001) and 0.165 mg/mL BCIP (5-Bromo-4-chloro-3-indolyl phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml vPBS (PBS, 46 mM sucrose, 10 mM glucose) containing EDTA-free protease inhibitors (Roche). The washed cells were pelleted by centrifugation (750xg ...
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bioRxiv - Immunology 2023Quote: Lungs were perfused with sterile PBS and the left lobes of the lungs were digested at 37°C with 630 µg/ml collagenase D (Roche) and 75 U/ml DNase I (Sigma) ...
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bioRxiv - Developmental Biology 2023Quote: ... Isolated tissue was cut in pieces of 2 cm and digested for 1h at 37°C in a HBSS solution containing 1% P/S and 2 U ml-1 Dispase II (Roche). Epidermal layer was separated from the dermis using two tweezers ...
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bioRxiv - Cancer Biology 2023Quote: ... the skin from euthanized neonatal mice of indicated genotypes was incubated with 250 units/mL dispase (Roche, Catalog No. 04942078001) in KSFM medium (Thermo Fisher ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were dissociated using a combination of enzymatic disruption using Liberase (Roche, 05 401 119 001, 5 μg/mL final), DNaseI (Sigma ...
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bioRxiv - Developmental Biology 2023Quote: ... Positive controls were generated by incubating some sections from control 3 dpf animals with recombinant DNAse I (400 U/ml; Roche) for 20 minutes at room temperature before the incubation in the reaction mix ...
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bioRxiv - Biophysics 2023Quote: Cell lysate powder from 12 L of cell culture was resuspended in 300 mL of room temperature eIF4G Lysis Buffer supplemented with 2 EDTA-free protease inhibitor tablets (Roche), 20mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... Cells were harvested by centrifugation at 7000 xg for 15 minutes and were lysed in 10 ml PBS supplemented with protease inhibitor (cOmplete, Mini, EDTA-free, Roche). NatT was purified by immunoprecipitation using Anti-FLAG magnetic beads (Merk ...
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bioRxiv - Physiology 2024Quote: Dissected tips of digits and foot pads of mice were minced and incubated in 0.25mg/mL liberase TL (Roche, 5401119001) for 45 min at 37 °C with pipetting every 10min ...
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bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
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bioRxiv - Neuroscience 2024Quote: ... The cell pellet was resuspended (20 mL/L culture volume) in lysis buffer (20 mM Tris pH 8, 150 mM NaCl, and protease inhibitor cocktail, Roche). These resuspended cells were disrupted using sonication (QSonica sonicator ...
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bioRxiv - Cancer Biology 2024Quote: ... in buffer (10 mM Tris-Cl pH8.0, .25 mM EDTA, 100mM NaCl, 0.5% SDS 1mg/ml proteinase K) (Roche Cat #03508838103) at 55 °C for 4 hours to remove proteins ...
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bioRxiv - Bioengineering 2024Quote: ... or 1 M NaCl (for ori designs), 20 µg/mL DNase (#A3778, ITW Reagents) and protease inhibitor cocktail (04693132001, Roche)) ...
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bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
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bioRxiv - Neuroscience 2024Quote: ... Stable cell lines were established by selection with Schneider’s Drosophila medium supplemented with 300µg/ml hygromycin (Roche, Palo Alto, CA).
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bioRxiv - Plant Biology 2024Quote: ... Total 2.5 g powder of each sample was resuspended in 30-ml 1% TFA with 250 μl protease inhibitor cocktail (Roche) and then centrifuged at 12,000 ×g for 20 min at 4℃ ...
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bioRxiv - Plant Biology 2024Quote: Seed histological sections were washed with Phosphate Buffered Saline solution (PBS) and digested with 1 mg/ml proteinase-K (Hoffmann-La Roche). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... The tissue was then washed twice in PBS with antibiotic and antimycotic and digested with 2mg/ml collagenase P (Roche) in DMEM (Gibco ...
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bioRxiv - Cancer Biology 2024Quote: Xenograft OS tumors were harvested and microdissected 12 d after 143B OS cell inoculation and digested with collagenase type I and II (1 mg/ml; Roche) digestion for 45 min ...
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bioRxiv - Molecular Biology 2024Quote: ... samples were washed three times with 10 ml of ice-cold PBS supplemented with Complete Protease Inhibitor (Roche, Basel, Switzerland), and the cells were resuspended in 1 ml of the same buffer ...
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bioRxiv - Immunology 2024Quote: ... whole pituitaries or separate posterior and anterior pituitaries were mechanically lysed by suspending and digested with 50 µg/ml DNase 1 (Roche) and 1 mg/ml Collagenase D (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... supplemented with a 20-fold excess of unlabeled oligonucleotide with the same sequence as the labeled one (to prevent reannealing) and 1 µl of Proteinase K (18 mg/ml) (Roche). After deproteination for 30 min at 37 °C ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...
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bioRxiv - Genomics 2024Quote: ... were digested for 1 hour at 37°C under 110 rpm shaking conditions in DMEM supplemented with 2 mg/mL collagenase D (Roche) and 0.4 mg/mL Dispase (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: ... The minced hearts were transferred to digestion buffer (20mM BDM, 1.5mg/ml Roche Collagenase/Dispase enzyme mix in Leibovitz L15 medium)(Roche, 10269638001)(cellgro ...
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bioRxiv - Cell Biology 2024Quote: ... dissected TA muscles were first digested with 10ml muscle digestion buffer (DMEM containing 1% penicillin/streptomycin, 0.125mg/ml Dispase II (Roche, 04942078001), and 10mg/ml Collagenase D (Roche ...
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bioRxiv - Plant Biology 2024Quote: ... A total of 5 mL of either test buffer supplemented with 1 mM phenylmethylsulfonyl fluoride and one protease inhibitor tablet (Roche) was then to resuspend the fine powder ...
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bioRxiv - Neuroscience 2024Quote: ... Tissue was dounce homogenized in a nuclear extraction buffer (10mM Tris, 146mM NaCl, 1mM CaCl2, 21mM MgCl2, 0.1% NP-40, 40u/mL Protector RNAse inhibitor (Roche 3335399001). Dounce homogenizer was washed with 4mL of a washing buffer (10mM Tris ...
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bioRxiv - Plant Biology 2024Quote: ... incubated three days at room temperature in the dark and then moved to selection media (CIM + Hygromycin (40 mg/ml final concentration, Roche) + Timentin (200 mg/ml final concentration ...
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bioRxiv - Cancer Biology 2021Quote: ... and estrous cycle phase were pooled in PBS and minced followed by enzymatic digestion in DMEM/F12 media containing 2 mg/ml Collagenase A (Roche #11088793001) and 100 U/ml Hyaluronidase (Sigma #H3506 ...
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bioRxiv - Cell Biology 2019Quote: ... pH 7.5) containing protease inhibitor cOmplete Tablets EASYpack or phosphatase inhibitor PhosSTOP EASYpack (each 1 tablet per 15 mL, Roche, Basel, Switzerland), and diluted in 6×SDS-PAGE sample loading buffer (Beyotime ...
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bioRxiv - Cell Biology 2020Quote: ... 1 mM sodium molybdate) and protease inhibitors (1 mini-Complete EDTA-free tablet per 10 ml lysis buffer; Roche Life Sciences)) and sonicated three times for 15 sec each with intermittent cooling on ice ...
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bioRxiv - Immunology 2021Quote: Single-cell suspensions of tumors were generated by dicing tumors with a razor blade followed by enzymatic digestion for 30 minutes at 37C in medium containing 1 mg/mL Collagenase D (Roche Diagnostics) and 1μg/mL DNase I (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2021Quote: ... PBMC were seeded at 1 × 106 cells/mL in erythroid differentiation-promoting medium based on StemSpan™ Serum-Free Expansion Medium (SFEM) supplemented with human recombinant EPO (2 U/ml, Roche), human recombinant stem cell factor (25 ng/ml ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The wells containing fixed cells were washed once with 1X PBS and stained with DAPI (50 μg/ml) for (Roche, 10236276001) 10 min at RT and protected from light ...
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bioRxiv - Neuroscience 2021Quote: ... Pooled tissue from each brain region was suspended in 6 ml of 0.32 M sucrose homogenization buffer (4 mM HEPES, 0.1 mM CaCl2, 1 mM MgCl2, plus Roche protease inhibitor tablet) and homogenized with a Teflon homogenizer using 10 strokes at 900 rpm ...