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Citations for Roche :
4601 - 4650 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Myelin was washed by dilution in HBSS and centrifuged at 400 x g for 5 min at 4 °C before suspending in 3 mL ice cold 0.32 M sucrose solution containing protease inhibitor cocktail (Roche). Next ...
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bioRxiv - Immunology 2022Quote: ... T cells were isolated by dissociating tumor tissue in the presence of collagenase D (1 mg/ml) (Roche-Merck) for 10 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 ml urea buffer (8M urea, 1mM EDTA, 10mM TrisHCl pH 7.5, 1 mM PMSF, 1x Roche protease inhibitor) was then added and the sample was gently mixed ...
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bioRxiv - Molecular Biology 2022Quote: ... cells from 100 mL exponential phase cultures resuspended in 1.5 mL lysis buffer (buffer W with added cOmplete Mini protease inhibitor cocktail, Roche) were lysed by repeated sonication (10×20 s ...
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bioRxiv - Biochemistry 2022Quote: ... Clarified supernatant was filtered through a 0.45 μM PES syringe filter and incubated with 10 mL of cOmplete His-Tag purification beads (Roche) for 1 h with gentle rocking ...
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bioRxiv - Biochemistry 2022Quote: ... The cell lysate was diluted to 0.8 mg/ml total protein concentration and 0.47% NP40 using dilution buffer (PBS plus protease inhibitor cocktail (Roche 04693159001) and precleared using Invitrogen™ Dynabeads™ MyOne™ Streptavidin T1 magnetic beads for 2-4 hours at 4°C during gentle rotation ...
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bioRxiv - Bioengineering 2022Quote: Mouse hearts were harvested from euthanized mice and enzymatically digested with collagenase A (1 mg/mL; Roche Life Science) and dispase (2.5 U/mL ...
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bioRxiv - Biophysics 2022Quote: ... Frozen bacteria containing full-length or ΔUBLΔUBA UBQLN constructs were resuspended in Buffer A (50 mM Tris pH 8, 1 mM MgCl2, 1 mM PMSF, and 0.2 mg/mL DNase I and Roche Complete Mini protease inhibitor cocktail) ...
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bioRxiv - Cancer Biology 2022Quote: ... and glands minced with surgical scissors before enzymatical dissociation for 1.5h in DMEM/F12 (1:1) supplemented with 2 mg mL−1 collagenase (Roche) + Gentamicin (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... finely chopped and incubated for 2 hours in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Genomics 2022Quote: ... the captured DNA was amplified for ten cycles using KAPA HiFi HotStart ReadyMix (6.25 ml, Roche, catalog number 7958935001) and purified in 1.8 x volume of Agencourt AMPure beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... Trim24 knockout TC-1 ES cells (background 129S6/SvEvTac) carrying an RMCE selection cassette (described previously82) were selected under hygromycin (250μg/ml, Roche) for 10 days ...
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bioRxiv - Neuroscience 2022Quote: ... 3 mL of Tris (pH = 8.5) and 3 tablets each of the protease/phosphatase inhibitors PhosStop and Complete Mini (Roche). Tissues were then lysed in Precellys Homogenizer Bertin (program 6 x 20s pulse ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were harvested by cell scraping into 2 ml of PBS with a protease inhibitor cocktail (Roche cOmplete 4693159001). The cell suspension was centrifuge at 200xg for 10 min ...
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bioRxiv - Physiology 2023Quote: ... enzymatic digestion was carried out for 20-25 min at 36 °C in the low-Ca2+ solution containing Liberase TH Research Grade (0.15 mg/mL; Roche) and elastase (0.5 mg/mL ...
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bioRxiv - Microbiology 2022Quote: ... Cells were lysed with lysis buffer (final concentration: 50 μg/ml lysostaphin, 0.8% NP-40, 1x protease inhibitor (Roche), 250 U/ml benzonase and 1 mM MgCl2 in PBS ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1.5 ml 5 M NaCl, 12.5 μL 2 M Spermidine, final volume brought to 50 ml with dH2O, complemented with one Roche Complete Protease Inhibitor EDTA-Free tablet) ...
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bioRxiv - Molecular Biology 2022Quote: ... the isolated skin was incubated overnight at 4 °C in a tube containing 10 mL of 1x Dispase (Roche) and 2x Pen/Strep (Sigma ...
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bioRxiv - Biophysics 2022Quote: ... The transcription mixture was incubated at 37 °C for 4 h and treated with 50U/mL DNAse I (Roche) at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Remaining red blood cells were resuspended in extraction buffer (50 mM Tris [pH8.0], 1 mM EDTA [pH8.0], 0.5% SDS and 1 mg / ml Proteinase K [Roche, Nutley, NJ]) and incubated overnight at 55°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... Hippocampus was then sliced with a scalpel and digested for 1 h at room temperature in HBSS supplemented with 0.5 mg/ml collagenase D (Roche) and 10 µg/ml DNase I (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2024Quote: ... kidney was thawed in 1 ml of hypotonic buffer (10 mM Tris pH 7.5, 150 mM sucrose including EDTA-free cOmplete protease inhibitor (Roche)) and homogenized using bead mill (5 cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK cells were lysed in 1xPBS pH 7.4 containing Protease Inhibitor Cocktail tablet (1 tablet/10 mL) (Roche Diagnostics) and separated in 4–16% gradient Native pre-cast gel (SERVA Electrophoresis GmbH) ...
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bioRxiv - Molecular Biology 2023Quote: ... MIO-M1 cells were transfected with 20 µg/ml of pEGFP or pEGFP-SIK2 vector using FugeneHD (Roche, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ZNF274 expression was induced with 1µg/ml doxycycline and verified via western blot with an anti-HA antibody (1:1000, ref.12013819001, Roche) and anti-beta actin antibody (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... Ganglia were then treated for 20 min at 37°C with 3 mg/ml collagenase (type I; Roche Diagnostics) and 3 mg/ml dispase II (Roche Diagnostics ...
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bioRxiv - Developmental Biology 2024Quote: ... ovaries were crosslinked in 250 µL YSS buffer (50 mM pH 8.0 Tris HCl, 50 mM NaCl, 0.1% NP40, 1 U/mL of RNAsin (Roche, 3335402001), protease inhibitor (Thermo Scientific ...
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bioRxiv - Immunology 2024Quote: ... followed by tissue digestion for 1 h at 37°C with Iscov’s digestion media containing 1 mg/ml Liberase TL (Roche), and 0.5 mg/ml DNAse (Thermo ...
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bioRxiv - Cell Biology 2024Quote: ... 50 µg of cryoground tissue powder was placed in a 1.5mL lobind tube and brought to 1 mL with lysis buffer (1% triton, 1x PBS, 1x Roche Complete protease inhibitor cocktail ...
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bioRxiv - Immunology 2024Quote: ... The digestion was stopped by adding one volume of 2X STOP Buffer (340mM NaCl, 20mM EDTA, 4mM EGTA, 0.02% Digitonin, 50ug/mL RNase A (Cat #3335399001, Roche), 50ug/mL Linear Acrylamide (Cat #K548 ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Immunology 2024Quote: ... at a ratio of 1:3 (cell:bead) in the presence of 2000 IU/mL recombinant human IL-2 (TECIMTM, Hoffman-La Roche provided by NCI repository ...
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bioRxiv - Genomics 2024Quote: ... whole blood (14.0 – 17.0 mL per subject) was collected using Cell-Free DNA Collection tubes (Roche Diagnostics; Rotkreuz, Switzerland), with no in-clinic processing (e.g. ...
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bioRxiv - Genetics 2023Quote: ... 10% Glycerol, 0.2% NP-40, 5mM MgCl2, 5mM DTT, containing one tablet per 10 ml of protease inhibitor cocktail, Roche). Cell debris was removed by centrifugation at 12 Krpm at 4°C for 20 min and the supernatant was recovered ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were subsequently washed with PBS three times and nuclei were counterstained with DAPI (2.5 μg ml−1, Roche) before mounting with Prolong Gold (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2023Quote: ... the mixture was incubated at 42 °C overnight and then added to 10 ml DIG-Easy hybridization solution (Roche).
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bioRxiv - Genomics 2023Quote: ... samples were minced and 0.15-0.2g of the minced tissue was transferred into the digestion mix (1.07 wU/ml Liberase DH (Roche, 5401054001), (70 U/ml ...
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bioRxiv - Immunology 2023Quote: ... euthanized mice were perfused with PBS and the left lung lobes digested for 1 h with collagenase D (1.5 mg/ml, Roche) and DNAse A (40 U/ml ...
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bioRxiv - Developmental Biology 2023Quote: ... The aqueous layer was then transferred to a siliconized Eppendorf tube followed by the addition of 2.0 mL glycogen (Roche) and 500 mL isopropanol (Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: Skin samples collected from the ventral neck area of 4-5 mice were minced in Hank’s balanced solution (HBSS) and incubated at 4°C overnight with 1.5mg/ml dispase II (Roche; 25766800). The next day ...
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bioRxiv - Immunology 2023Quote: ... Popliteal lymph nodes were harvested from immunized mice 1 day later and digested with collagenase D (2.5 mg/mL, Roche) in RPMI-1640 medium supplemented with 10% FBS for 30 min at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Cerebral cortex was dissociated by trituration in DMEM/F12 containing 10% FBS and 20 µg/ml DNase I (Roche) using a fire-polished Pasteur pipette ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed by sonication in 50 mL lysis buffer (20 mM Tris pH 8.5, 200 mM NaCl, 10% glycerol, 1 mM PMSF, 2x Roche protease inhibitor tablets ...
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bioRxiv - Microbiology 2022Quote: ... according to the manufacturer’s instructions and maintained in RPMI GlutaMax supplemented with 10% FCS and 30 U/ml recombinant IL-2 (Roche).
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bioRxiv - Biochemistry 2022Quote: Cells were thawed and resuspended in 250 mL of ice-cold hypotonic lysis buffer (10 mM HEPES, pH 7.5, containing Roche protease inhibitor tablets and 0.1 mU/mL apyrase to prevent separation of the GPCR-miniG complex) ...
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bioRxiv - Molecular Biology 2022Quote: ... flow cytometry analysis was performed on cell suspensions from kidney homogenates that were digested with 0.2 mg/ml liberase (Roche) and 0.1 mg/ml DNase I (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% NP40, 150 mM NaCl, 2 mM EDTA, 50 mM NaF, 0.1mM Na3VO4, 4 μg/ml leupeptin, one Roche cOmplete™ protease inhibitor tablet ...
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bioRxiv - Biochemistry 2023Quote: ... 0.75%/0.15% dodecyl maltoside/cholesteryl hemisuccinate (DDM/CHS) with 2 mg/ml iodoacetamide and a Protease Inhibitor tablet (Roche). After 4 hours ...
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bioRxiv - Molecular Biology 2023Quote: ... was purified via Immobilized Metal Affinity Chromatography (IMAC) using a prepacked 5 mL cOmpleteTM His-Tag purification column (Roche) equilibrated in HEPES-buffered saline buffer (HBS ...
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bioRxiv - Microbiology 2023Quote: ... bacterial cell pellets were resuspended in 1.5 ml of lysis buffer (25 mM Tris pH 7.5, 150 mM NaCl, benzonase, EDTA-free protease inhibitor cocktails (ROCHE)) and disrupted at 4°C with 0.1 mm glass beads and using a PRECELLYS 24 homogenizer ...