Labshake search
Citations for Roche :
4601 - 4650 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2022Quote: ... The sheared fragments were treated with end repair and A-tailing using the KAPA-Illumina library creation kit (KAPA biosystems) followed by immobilization of mate pair fragments on strepavidin beads (Invitrogen) ...
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bioRxiv - Developmental Biology 2022Quote: ... qRT-PCR was performed using an Eva Green 2X qPCR MasterMix-No Dye kit (Abm, MasterMix-S) on a LightCycler 480 instrument (Roche). Relative gene expression was analyzed based on the 2-ΔΔCt method with β-actin as internal controls ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative real-time PCR (qRT-PCR) was performed with the LightCycler 480 SYBR Green I Master kit (Cat. 04887352001, Roche). Primers for pmp-3 and ACTIN were used as housekeeping for C ...
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bioRxiv - Cancer Biology 2022Quote: Viability and cell growth ratios were determined analyzing cell density by MTT using the Cell Proliferation Kit I (Roche, 11465007001) in DND41 cells treated with EX-527 (10-6 to 10-3 M ...
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bioRxiv - Cell Biology 2022Quote: ... the RNA concentration was determined and 1 µg RNA was used for the cDNA synthesis according to the Transcriptor First Strand cDNA Synthesis kit (Roche). The expression of the following genes FTSH7 (AT3G47060) ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s protocol. Quantitative PCR was performed using SensiMixTM II Probe Kit (Cat#. BIO-83005) with Universal ProbeLibrary (Roche Diagnostics), and the CFX96 Touch System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2022Quote: ... The KAPA Stranded mRNA Sample Preparation Kit was used to prepare polyA enriched RNA libraries (Kapa Biosystems, Wilmington, MA, USA). Trimmomatic [73] (version 0.36 ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were then purified and reverse transcribed with digoxigenin labeling using a DIG RNA labelling kit (Roche, Switzerland). For dissected adult pituitary or 5-8 dpf larvae ...
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bioRxiv - Pathology 2020Quote: ... Total and small RNA (containing microRNA) were extracted from MΦ using the Roche High Pure miRNA Isolation kit (Roche, Milan) or the Quick-RNA MicroPrep from Zymo Research (Irvine ...
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bioRxiv - Cell Biology 2020Quote: ... Stranded RNA-seq libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase (HMR) and KAPA Unique Dual-Indexed Adapters (Roche) according to instructions provided by the manufacturer ...
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bioRxiv - Cancer Biology 2020Quote: ... The concentration of each library was determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (KAPA Biosystems/Roche) to produce appropriate cluster counts Illumina NovaSeq6000 instrument ...
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bioRxiv - Microbiology 2021Quote: Alkali-labile digoxigenin labelled deoxyuridine triphosphate (DIG-dUTP) was incorporated in Southern blot probes by PCR using PCR DIG Probe Synthesis kit (Roche) with the following specifications (refer to sequences in Supplementary table 3) ...
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bioRxiv - Plant Biology 2021Quote: ... A PCR master mix was prepared with the LightCycler 480 SYBR Green I Master Kit (Roche Applied Science, Mannheim, Germany). Each reaction was performed in a total volume of 10 μL ...
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bioRxiv - Microbiology 2020Quote: The cell viability of circPSD3 siRNA treated cells was determined at day 2 post-transfection using Cell Proliferation Kit I (MTT, Roche) as previously described [59].
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bioRxiv - Molecular Biology 2021Quote: Serum albumin measurements were determined using a commercial serum albumin kit (Alpha Laboratories Ltd., Eastleigh, UK) adapted for use on Cobas Mira analyser (Roche Diagnostics Ltd ...
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bioRxiv - Developmental Biology 2021Quote: Genomic DNA was extracted from 3 week old tail or ear biopsies using KAPA Mouse DNA Extraction Kit (KAPA Biosystems) or High Pure PCR Template Kit (Roche).
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bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Labelling efficiencies were tested by dot blotting with anti-digoxigenin alkaline phosphatase conjugate and CSPD chemiluminescence substrate for alkaline phosphatase from DIG High Prime DNA Labelling and Detection Starter Kit II (Roche) according to the manufacturer`s protocol ...
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bioRxiv - Immunology 2020Quote: ... 2.7 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK 8504). Samples were barcoded and used for 50bp/50bp paired end runs with the TruSeq SBS Kit v4 (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... RT-qPCR was performed using a Bio-Rad sequence detection system according to the manufacturer’s instructions using a double-stranded DNA-specific SYBR Green Premix Ex TaqTM II Kit (Roche, Switzerland). Experiments were conducted in duplicate in three independent assays ...
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bioRxiv - Molecular Biology 2020Quote: ... Intact RNA was depleted of rRNA and prepared for sequencing using the KAPA RNA HyperPrep Kit with RiboErase (Kapa Biosystems). Libraries were sequenced on the NextSeq 500 (Illumina) ...
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bioRxiv - Synthetic Biology 2020Quote: ... Library quality control and quantification were performed using the KAPA Library Quantification Kit for Illumina platforms (Kapa Biosystems, Massachusetts, USA) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2021Quote: ... 10.6 kbp and 16.4 kbp runs were constructed using the Oxford Nanopore Technologies (ONT) SQK-LSK109 kit according to the manufacturer’s instructions except that Kapa beads (Roche, UK) were used to perform the clean up steps rather than Ampure XP beads ...
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bioRxiv - Genetics 2020Quote: ... One short-insert WGS library was constructed using the Kapa Hyper Prep Kit (KR0961) from Kapa Biosystems (Woburn, MA, USA) with 150 ng of sheared DNA as input following standard manufacturer’s protocol with 10 cycles of PCR ...
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bioRxiv - Physiology 2021Quote: ... All mice were genotyped by polymerase chain reaction (PCR) using KAPA Taq PCR Kits (Kapa Biosystems, Inc., Wilmington, MA, USA). Specific primers purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Physiology 2020Quote: 1 μg RNA was reverse transcribed into cDNA with Oligo(d)T primers using the Transcriptor First Strand cDNA Synthesis kit (Roche). The qRT-PCR was performed with the LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Physiology 2021Quote: ... cDNA libraries were validated using high sensitivity DNA chip on the Agilent 2100 Bioanalyzer and quantified using the KAPA Library Quantification Kit (Roche). Indexed libraries were pooled and sequenced with a 50-bp single-end protocol on a HiSeq4000 platform (Illumina ...
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bioRxiv - Molecular Biology 2020Quote: ... Only one sample was obtained per patient: one portion of the sample was extracted using the High Pure Viral RNA Kit (Roche), and another portion of the same sample was extracted using the acid pH method ...
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bioRxiv - Microbiology 2020Quote: ... Total RNAs were extracted from 200 μl of mice sera using a High Pure viral RNA kit (Roche Applied Science) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were generated by random shearing of genomic DNA followed by ligation of custom adapters using the Kapa HyperPrep Kit (Roche). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... The resulting RNA was used as a matrix for reverse transcription using the AMV cDNA first strand synthesis kit (Roche). The produced cDNA was used to assess ecpD expression as well as 16S rRNA gene as a control.
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bioRxiv - Genomics 2020Quote: ... These DNA fragments were labeled with digoxigenin (DIG) using a DIG High Prime DNA Labeling and Detection Starter Kit II (Roche), and signals were detected according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2021Quote: Pitx1 WISH were performed on 40-45 somite stage mouse embryos (E12.5) using a digoxigenin-labeled Pitx1 antisense riboprobe transcribed from a cloned Pitx1 probe (PCR DIG Probe Synthesis Kit, Roche), as previously described in (Kragesteen et al ...
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bioRxiv - Plant Biology 2021Quote: ... Antisense or sense strand digoxygenin-UTP labeled RNA was generated for PEPCK and NADP-ME using a DIG RNA labeling kit (Roche). For PEPCK hybridizations ...
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bioRxiv - Immunology 2021Quote: ... The qPCR reactions were performed in triplicate for each cDNA sample using the Thunderbird Next SYBR qPCR Mix kit (Toyobo) on Light Cycler 96 (Roche). The primers used were the followings ...
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bioRxiv - Cancer Biology 2021Quote: ... RNA was extracted for tissues with sufficient tissue and bulk RNAseq libraries were prepared with KAPA mRNA HyperPrep kit (Roche). Bulk RNA sequencing data was processed with the same pipeline as the GLASS samples.
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bioRxiv - Neuroscience 2021Quote: Digoxigenin RNA-labeled or Fluorescein RNA-labeled probes were transcribed in vitro using the RNA Labeling Kit (Roche Diagnostics Corporation) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche). Diluted cDNAs (1:5 ...
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bioRxiv - Cancer Biology 2020Quote: ... The short-insert paired-end libraries for the whole genome sequencing were prepared with KAPA HyperPrep kit (Roche Kapa Biosystems) with some modifications ...
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bioRxiv - Cancer Biology 2020Quote: ... The short-insert paired-end libraries for the whole genome sequencing were prepared with KAPA HyperPrep kit (Roche Kapa Biosystems) with some modifications ...
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bioRxiv - Genetics 2021Quote: We extracted DNA from nail samples using the ISOHAIR (Nippon Gene) and oral mucosa samples using the High Pure PCR Template Preparation Kit (Roche).
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bioRxiv - Genomics 2020Quote: Barcoded 2×300 bp metagenomic libraries were prepared with the KAPA Hyperplus Library Preparation kit (KAPA Biosystems, Wilmington, MA, US) and sequenced on an Illumina MiSeq system at the National Oceanography Centre (Southampton ...
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Cancer Biology 2021Quote: Reverse transcription for qPCR analysis was done with the first strand synthesis kit (Fermentas) using oligo (dT)18 as a primer and qPCR was carried out with LightCycler 96 (Roche) using FastStart Essential DNA Green Master Mix (Roche) ...
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bioRxiv - Cancer Biology 2021Quote: The in vitro synthesis of digoxigenin (DIG)-labeled antisense SatIII probe (158 bp SatIII repeat cloned in 1μg of PGEM-2-98 construct) with T7 RNA polymerase was carried out using the DIG labeling kit from Roche Products Pvt ...
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bioRxiv - Cancer Biology 2021Quote: ... libraries were prepared in an automated fashion using an Agilent Bravo robot with aKAPA Standard mRNA-Seq Kit (KAPA BIOSYSTEMS). In-house sequencing adaptors were ligated to 100-300 bp fragments of input dsDNA ...
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bioRxiv - Immunology 2020Quote: ... RT-qPCR was performed using the LightCycler 480 Probe Master Kit and the LightCycler 480 II system (Roche Applied Science) as described previously92 ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Microbiology 2020Quote: ... Illumina compatible Indexed libraries were prepared from 12ng of Diagenode Biorupter Pico sheared ChIP DNA using the KAPA Hyper Library Preparation Kit (Roche). Libraries were enriched with 2 cycles of PCR then assessed for size distribution using the 4200 TapeStation High Sensitivity D1000 ScreenTape (Agilent Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... 1μl of cDNA diluted with 10-fold MQ was mixed with KAPA SYBR Fast qPCR kit and amplified with Lightcycler 96 (Roche; 95℃ 5 min ...