Labshake search
Citations for Roche :
4401 - 4450 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Sequencing was done using Illumina HiSeq X with sequencing shotgun library preparation using the KAPA HyperPrep Kit (Kapa Biosystems) following the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2023Quote: ... TUNEL staining was performed as in (46) using the TMR red in situ cell death detection kit (Roche, 12156792910).
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (1Lμg) was used to generate RNA-seq library with KAPA HyperPrep RNA Kits with RiboErase (Roche KK8560) using 8 cycles of PCR amplification ...
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bioRxiv - Systems Biology 2023Quote: ... RNA libraries were prepared for paired-end sequencing using the KAPA Stranded RNA-Seq Library Preparation kit (Kapa Biosystems). Libraries were sequenced on a NextSeq system (Illumina ...
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bioRxiv - Molecular Biology 2023Quote: ... Pre-hybridization was done at 68 °C for 1 h and using DIG Northern Blot Starter KIT (12039672910, Roche). DIG-labeled in vitro transcribed lncMtDloop ...
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bioRxiv - Developmental Biology 2023Quote: ... The molarity of adaptor-modified molecules was defined by a qPCR with a KAPA Library Quantification kit (Kapa Biosystems). Individual libraries were normalized to 10nM ...
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bioRxiv - Genomics 2023Quote: Carver Biotechnology Center at the University of Illinois at Urbana-Champaign with the Hyper Library Construction Kit (Kapa Biosystems) to obtain an average library fragment size of 500bp ...
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bioRxiv - Microbiology 2023Quote: ... The PCR DIG Probe Synthesis kit for probe synthesis and the CSPD luminescent detection kit for visualization of the bound probe were used according to the manufacturer’s instructions (Roche Molecular Biochemicals ...
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bioRxiv - Immunology 2023Quote: ... Library concentrations were also assessed by qPCR using the KAPA Library Quantification Kit (Complete, Universal) (Kapa Biosystems, Woburn, MA). Pooled libraries were sequenced on an Illumina NovaSeq 6000 using 150bp PE reads (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... we performed target-capture using a custom-designed probe kit with a SeqCap EZ Workflow (Roche, Indianapolis, IN, USA), which was designed to capture the entire nuclear genome of S ...
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bioRxiv - Cancer Biology 2023Quote: RNA extraction from A549 cells grown in 6-well plates was done using High Pure RNA isolation kit (Roche). Transcriptor First strand kit (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The concentration of each captured library pool was accurately determined through qPCR utilizing the KAPA library Quantification Kit according to the manufacturer’s protocol (Roche) to produce cluster counts appropriate for the Illumina NovaSeq-6000 instrument ...
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bioRxiv - Cell Biology 2023Quote: ... The ribodepletion method of library preparation for RNA sequencing was performed using the Total-RNASeq RiboErase Kit (KAPA Biosystems). All RNA samples were loaded two separate 2-lane flow cells and sequenced using an Illumina HiSeq 2500 system generating 51 bp paired-end reads ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 20 x 106 cells were used for isolating genomic DNA with High Pure PCR Template Preparation Kit (Roche) as described by the manufacturer ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Illumina libraries were prepared using a modified KAPA protocol with the KAPA LTP Library Preparation Kit (Roche, Basel, Switzerland) with adaptor ligation and adaptor fill-in reactions based on Meyer and Kircher (2010) ...
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bioRxiv - Developmental Biology 2023Quote: ... We synthesized digoxigenin (DIG)-labeled sense and antisense RNA probes using a DIG RNA Labeling Kit (Roche, Basel, Switzerland) after digestion with the appropriate restriction enzymes BamHⅠ-HF ...
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bioRxiv - Physiology 2023Quote: ... Cell apoptosis was measured using an in situ cell death detection kit and following the manufacturer protocol (Roche, 12156792910). Images were obtained using an inverted fluorescent microscope (Leica DMi8 ...
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bioRxiv - Cell Biology 2023Quote: MTT activity was determined using the Cell Proliferation Kit I according to the recommendations of the manufacturer (Roche, Spain). Optical density was determined at 575[nm with a reference wavelength of 690[nm using a Varioskan Flash spectrophotometer (Thermo Scientific ...
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bioRxiv - Developmental Biology 2023Quote: Genotyping of ESCs and mouse tails were performed by PCR using KAPA Mouse Genotyping Kit (Roche, Cat. No. KK7302). Specific protocol followed the reagent instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The U6-sgRNA cassette was then amplified by PCR using the KAPA HIFI Hot Start PCR kit (Roche, KK2502) and different tagged primers required for the subsequent Illumina sequencing ...
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bioRxiv - Neuroscience 2024Quote: ... Cell death was evaluated separately using the Fluorescence In Situ Cell Death Detection kit (Roche Diagnostic, Indianapolis, IN, US). Brain sections were analyzed for DNA strand breakage using Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries for RNA-seq were prepared with KAPA Stranded mRNA-Seq poly(A) selected kit (KAPA Biosystems, Wilmington, MA) using 250 ng toal RNAs for each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems, #KK8504) using 8 cycles of PCR ...
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bioRxiv - Microbiology 2024Quote: ... Libraries were constructed from approximately 100 ng of genomic DNA using the Kappa HyperPrep Kit (Roche, Pleasanton, CA, USA) with mechanical shearing (Covaris ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA was purified with High Pure Viral Nucleic Acid Kit according to the manufacturer’s instruction (ROCHE, Mannheim, Germany). Viral RNA was amplified by LightMix® Modular Sarbecovirus E-gene (Roche ...
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bioRxiv - Immunology 2024Quote: ... adhering to the manufacturer’s recommendations for low-input DNA (MyBaits v5 kit Arbor Biosciences) and using KAPA Universal Enhancing Oligos ((Roche) to prevent non-specific binding ...
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bioRxiv - Genomics 2024Quote: Total RNA and DNA:RNA hybrid samples were reverse transcribed into complementary DNA (cDNA) using Evoscript universal cDNA Master Kit (Roche, Germany ...
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bioRxiv - Developmental Biology 2024Quote: ... Digoxigenin labeled antisense riboprobes were synthesized by in vitro transcription using a DIG RNA labeling kit (Roche, Cat#11277073910), followed by purification using Micro Bio-Spin P-30 gel columns (Bio-Rad ...
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bioRxiv - Plant Biology 2024Quote: The labeling of probes with digoxigenin was performed using the 2nd generation DIG Gel Shift EMSA kit (Roche, USA), following the instructions provided by the manufacturer ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA fragmentation was visualized by using an in situ cell death detection kit (Fluorescein; Cat #, 11684795910; Roche, Basel, Switzerland) following the manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... On the following day 10 µl MTT labeling reagent (5 mg/ml) of the Cell Proliferation Kit I (Roche) was added and cells were incubated at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting polyA RNA was used as input into the KAPA RNA Hyper prep RNAseq library prep kit (Roche). Libraries were prepared with dual-index adapters ...
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bioRxiv - Immunology 2024Quote: ... Adapter ligation and indexing PCR was performed on these amplicons using KAPA HTP Library Preparation Kit (KK8234, Kapa biosystems) for multiplexing and sequencing on an Illumina HiSeq5 platform.
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated from A549 cells under different treatment conditions using the High Pure RNA Isolation Kit (Roche), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... pre-capture libraries were prepared from up to 100 ng of input DNA using the KAPA Hyperplus kit (Roche) and TruSeq adapters and barcoded primers (Illumina) ...
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bioRxiv - Genomics 2024Quote: ... EM-seq and WGBS libraries were quantified using the KAPA library quantification kit (KK4854; Roche Molecular Systems, Pleasanton, CA) and pooled in equimolar amounts ...
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bioRxiv - Plant Biology 2024Quote: ... The final libraries were pooled equimolarly and quantified by qPCR using a Kapa Library Quantification Kit (Roche Diagnostics 7960204001).
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bioRxiv - Microbiology 2024Quote: ... The total RNAs were converted into individually barcoded polyadenylated RNAseq libraries with the Kapa HyperPrep mRNA kit (Roche, CA), with prior removal of rRNAs with the FastSelect Bacteria kit (Qiagen ...
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bioRxiv - Evolutionary Biology 2024Quote: A PCR free library was prepared following the Kapa Hyper Prep Kit procedure provided by Roche (Roche, Basel, Switzerland). The library was sequenced on a paired-end mode with an Illumina NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... whole cell lysates were harvested to determine protein expression levels of CAT using CAT ELISA kit (Roche, Sydney, Australia). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... and 1.25 µL of 2.5 µM indexing adapter from the KAPA Dual-Indexed Adapter Kit (Kapa Biosystems, MA, USA). The samples were incubated at 25°C for 15 min ...
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bioRxiv - Neuroscience 2024Quote: Digoxigenin-labeled RNA antisense probes and corresponding sense probes were synthesized using DIG RNA Labelling Kit (Roche, Basel, Switzerland) and the specific primers are listed in Supplementary Table 1 ...
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bioRxiv - Microbiology 2024Quote: ... The library pool was quantified by qPCR using a Kapa Library Quant RT-qPCR kit (Kapa/Roche, Basel, Switzerland) and sequenced on an Illumina NovaSeq 6000 instrument with paired-end 150 bp reads (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s instructions and the complementary DNA (cDNA) was synthesized using Transcriptor First Strand cDNA Synthesis kit (Roche). Synthesized cDNA was used for the amplification of gene of interest by PCR ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the concentration measured by qPCR with the KAPA Library Quant Kit (Kapa Biosystems Pty, Cape Town, South Africa). Sequencing consisted of paired-end sequencing with 150 bp read length and was performed by the SNP&SEQ Technology Platform in Uppsala on a NovaSeq 6000 with a S4 flowcell and v1.5 sequencing chemistry.
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bioRxiv - Genomics 2024Quote: ... Tasmanian Ophthalmic Biobank samples were prepared for sequencing using the KAPA Hyper PCR-free sequencing library preparation kit (Roche) while BioHEART samples were prepared using the DNA PCR-free sequencing library preparation kit (Illumina).
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bioRxiv - Molecular Biology 2024Quote: ... The purified DNA fragments were prepared according to the protocol of the KAPA LTP Library Preparation kit (KR0453, Roche) before sequencing ...
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bioRxiv - Microbiology 2024Quote: ... Influenza A Subtyping Kit with the SuperScript III Platinum One-Step qRT-PCR Kit System on the MagNA Pure LC 2.0 platform (Roche).
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bioRxiv - Neuroscience 2024Quote: ... The genotyping of these mice was performed with a KAPA2G (15) Fast Hot Start DNA polymerase kit (KAPA Biosystems). The following primers were used to identify Pink1 WT and KO mice ...
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bioRxiv - Plant Biology 2024Quote: ... The RT-qPCR reaction was performed using the FastStart Essential DNA Green Master Kit (Roche Diagnostics GmbH, Mannheim, Germany) and the LightCycler 96 thermal cycler (Roche ...