Labshake search
Citations for Roche :
4301 - 4350 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The library was constructed following the manufacturer protocol of the KAPA LTP Library Preparation Kit (KAPA Biosystems, Roche, Switzerland). ChIP DNA libraries were ligated with the Bioo scientific barcoded adaptors (BIOO Scientific ...
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bioRxiv - Genetics 2020Quote: ... Southern blot was performed according to the manufacturer’s protocol of DIG HIGH prime DNA labeling and detection starter kit 1 (Roche). 10 µg aliquot of genomic DNA was digested overnight with DraIII ...
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bioRxiv - Genetics 2020Quote: ... RNA-seq libraries were prepared from high-quality RNA samples (RIN scores >9.0) with KAPA Stranded RNA-seq Kit with RiboErase (Roche). Paired 150-bp reads (21.2 - 118.8 million reads per sample ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Detection of the respective bands was carried out by the Dig DNA labeling and detection kit (Roche, Penzberg, Germany) according to the manufacturers instructions.
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bioRxiv - Evolutionary Biology 2021Quote: ... Antisense Digoxygenin-UTP riboprobes were synthesized using SP6 or T7 RNA polymerases and the DIG RNA Labeling kit (Roche).
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bioRxiv - Cell Biology 2021Quote: ... RNA-seq libraries were prepared from 100 ng of total RNA using RNA HyerPrep kit with RiboErase (Kapa Biosystems). Sequencing was performed at the MD Anderson core facility equipped with an Illumina HiSeq 4000 instrument ...
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bioRxiv - Genomics 2021Quote: ... We performed capture-based target enrichment using the SeqCap EZ Hybridization and Wash Kit (Roche NimbleGen, Madison, WI, U.S.). We then used the sequencing platform ...
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bioRxiv - Genomics 2021Quote: ... Pre-capture libraries were prepared from up to 100 ng input genomic DNA using the Kapa Hyperplus kit (Roche), using a fragmentation time of 8 minutes and standard-chemistry end repair/A-tailing ...
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bioRxiv - Genomics 2020Quote: ... The fragmented DNA was then ligated with dual-indices using a KAPA Hyper prep PCR-free library kit (Roche) according to the manufacturer’s directions ...
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bioRxiv - Zoology 2021Quote: ... nasal and tonsil swab) to a final elution volume of 80ul using MagNA Pure LC RNA isolation kit (Roche) and the KingsFisher Flex 96 robot (Thermofisher) ...
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bioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing libraries were loaded on a NovaSeq 6000 (Illumina ...
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bioRxiv - Genomics 2021Quote: ... Library preparation was carried out with 500 ng sheared DNA using Kapa Hyper library prep kit (Roche, Basel, Switzerland). Barcodes were added using the Illumina UD 96 index kit (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... The isotypes of anti-Drosophila antibodies were determined by the IsoStrip™ Antibody Isotyping Kit (11493027001 Roche, Basel, Switzerland) according to the instructions of the manufacturer.
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bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... 1 μg of total RNA was reverse transcribed using a Transcriptor First Strand cDNA synthesis kit (Roche Applied Science) and the provided random hexamer primers for the mix preparation ...
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bioRxiv - Genomics 2020Quote: ... An Illumina compatible NGS library was then prepared with 50ng of genomic DNA using the KAPA HyperPlus kit (Roche). Sequencing was performed on a NextSeq500 instrument (Illumina) ...
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bioRxiv - Immunology 2020Quote: ... Stranded RNA libraries were prepared with 100ng total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche). RNA sequencing was performed on Illumina NovaSeq 6000 system (Illumina) ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from BHI broth cultures using the High Pure PCR template preparation kit (Roche, Potsdam, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2020Quote: ... The library concentration was determined by qPCR using the Library Quantification Kit Illumina Platforms (Kapa Biosystems, Boston, MA, USA). An equimolar pool of the barcoded libraries was made and the pool was sequencing on a HiSeq 2500 system (Illumina ...
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bioRxiv - Developmental Biology 2020Quote: ... stranded RNA libraries were prepared with 100ng total RNA using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche). RNA sequencing was performed on Illumina NovaSeq 6000 system (Illumina).
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bioRxiv - Genomics 2020Quote: ... the total DNA was sheared to ~500 bp size fragments and processed using KAPA LTP Library Preparation Kit (Roche Sequencing Solutions ...
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bioRxiv - Genomics 2021Quote: ... Sequencing libraries were prepared from these diluted samples using a miniaturized version of the KAPA HyperPlus kit (Roche, KK8514) with Illumina-compatible KAPA Unique Dual-Indexed Adapters (Roche ...
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bioRxiv - Neuroscience 2021Quote: ... antisense and sense digoxogenin (DIG)-labeled RNA probes were generated using the DIG RNA labeling kit (Roche Diagnostics, Germany), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... two PCR reactions were performed with PCR anchor primer (included in the 2nd Generation 5’/3’ RACE Kit, Roche) and GB3 (oligo 61 ...
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bioRxiv - Immunology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Molecular Biology 2022Quote: The library of mtDNA was prepared from 500 ng mitochondrial DNA with a KAPA HyperPlus Kit PRC-free (Roche), according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: NGS libraries from DRIP DNA and inputs were constructed according to the manufacturer’s instructions (KAPA Hyper Prep Kit: Roche, cat ...
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bioRxiv - Cancer Biology 2023Quote: ... Total RNA (3–4 μg) was used to prepare cDNA with the Transcriptor First-Strand cDNA synthesis kit (Roche). qPCR was performed with TaqMan gene expression assay primers (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... Library concentration was assessed using both Qubit fluorometric quantification and the qPCR-based KAPA Library Quantification Kit (Roche, KK4824). Appropriate fragment size distributions were confirmed by running each sample on an Agilent Bioanalyzer using the DNA High-Sensitivity Kit (Agilent ...
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bioRxiv - Genetics 2023Quote: ... The molar concentration of the pool was measured using the KAPA Illumina Library Quantification Kit (KAPA Biosystems, Wilmington, MA) and the pool was sequenced on one lane of the Illumina HiSeq 4000 sequencer with a 75 bp Paired-End SBS chemistry (Illumina).
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bioRxiv - Genetics 2023Quote: ... Real time PCR was performed on the cDNA using KAPA SYBR FAST qPCR Master Mix (2X) kit (Roche, #KK4618) with intron spanning primers in a QuantStudio 3 Real-time PCR system ...
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bioRxiv - Molecular Biology 2023Quote: ... Highly sensitive DNAprobes labelled with digoxigenin-dUTP were generated using the PCR digoxigenin probe synthesis kit (Roche Applied Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... Libraries were quantified by qPCR using the Library Quantification Kit for Illumina sequencing platforms (cat#: KK4824, KAPA Biosystems, Boston), using 7900HT Real Time PCR System (Applied Biosystems) ...
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bioRxiv - Immunology 2023Quote: ... 10 ng of amplified cDNA was used to prepare libraries with the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) using 8 cycles of PCR ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 6ng of ChIP DNA was processed for library preparation for Next Generation Sequencing by using Kapa kit (Roche) following the manufacturer’s instruction with one modification ...
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bioRxiv - Neuroscience 2023Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). Sections were treated with Discovery CC1 cell conditioning solution at 95°C for 60 min followed by a low concentration of protease (Protease 3 ...
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bioRxiv - Microbiology 2023Quote: ... stranded mRNA-seq libraries were prepared from total RNA (100 ng) using the KAPA Stranded mRNA-Seq kit (Roche) according to the manufacturer’s standard protocol ...
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bioRxiv - Genomics 2023Quote: TP pre-capture libraries were prepared as previously described [15] using the KAPA HyperPlus kit with UDI adapters (Roche). 17.5µL DNA was used as input for fragmentation for eight minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... B-16 cells were collected after 48 hours of transfection and RNA was extracted using (Roche, RNA isolation Kit). Total RNA was quantified on a nanodrop (Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... Poly(A)-enriched next-generation sequencing library construction was performed using the KAPA mRNA Hyper Prep Kit (KAPA Biosystems) with 100 ng of input total RNA and 15 amplification cycles according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... Quantitative PCR was carried out on standards and test samples using the LightCycler 480 Probes Master kit (Roche, 04707494001) with inverted terminal repeat probe and primer sequences indicated in Table S4.
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bioRxiv - Cell Biology 2023Quote: ... In vitro transcription (IVT) was performed using T7 mMassage mMachine kit (invitrogen) and purified using RNA purification column (Roche). MCU-IVT was injected at single cell stage and embryos were cultured in E3 water ...
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bioRxiv - Bioengineering 2023Quote: ... of the VH gene was subjected to second-strand synthesis in a 25 μl reaction volume using IGHV gene-specific primers (primer No. 23–32, 7.5 μl) and a KAPA Biosystems kit (Roche). The reaction conditions were as follows ...
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bioRxiv - Neuroscience 2022Quote: ... and Ventana proprietary detection reagents utilizing 3,3′-diaminobenzidine tetrahydrochloride as the chromogen (DAB Map Detection Kit; Roche Tissue Diagnostics). For haematoxylin and eosin (H&E ...
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bioRxiv - Physiology 2022Quote: Both ATP release and intracellular ATP from isolated mesenteric arteries were performed using a ATP Bioluminescence HSII kit (Roche) kit as previously described.3
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bioRxiv - Molecular Biology 2022Quote: The psyllid midguts were diagnosed for cell-death or apoptosis using In situ cell-detection kit TMR-red (Roche) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Viral RNA isolation was performed using the MagNA Pure LC system (Total nucleic acid isolation kit, Roche Molecular System) or the EZ2 Connect system (EZ1&2 Virus Mini Kit v2.0 ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR amplification was performed in two technical replicates using KAPA Hifi Hotstart PCR kit (Kapa Biosystems, MA, USA) via a Bio-Rad CFX96 thermal cycler (Bio-Rad Laboratories ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... We prepared RNA libraries using the KAPA mRNA HyperPrep Kit and Unique Dual-Indexed Adaptors (KAPA Biosystems; Massachusetts, USA), with 200 ng of RNA as input ...
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bioRxiv - Molecular Biology 2024Quote: ... The preparation of next generation sequencing libraries was done using the KAPA HyperPrep ChIP Library kit (Roche Sequencing solutions) at the molecular biology and functional genomics platform of the Institut de Recherche Clinique de Montréal (IRCM) ...