Labshake search
Citations for Roche :
4201 - 4250 of 5547 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... genomic DNA libraries were generated from amplified samples using the KAPA Library Preparation Kit (Kapa Biosystems, Woburn, MA), and then fragmented to approximately 200 base pair (bp ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from PBMCs stored in RNAlater® with a High Pure RNA Isolation Kit (Roche) via a modified protocol ...
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bioRxiv - Genomics 2024Quote: Genomic DNA (gDNA) was isolated from PBMCs via a High Pure PCR Template Preparation Kit (Roche, Penzberg, Germany). The concentration (ng/μL ...
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bioRxiv - Immunology 2024Quote: ... for RNA extraction followed by library preparation using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche Diagnostics) and next-generation sequencing on the Illumina NextSeq 500 Instrument using a High Output 150 cycle kit ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was performed using SYBR green premix pro Taq HS qPCR kit with a LightCycler 96 instrument (Roche) and PCR was performed using 2×TransTaq-T PCR SuperMix ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were then pooled at equimolar concentrations and quantified by qPCR using a KAPA library quantification kit (Roche). Sequencing was performed on a NovaSeq 6000 instrument (Illumina ...
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bioRxiv - Bioengineering 2024Quote: ... lactate dehydrogenase (LDH) activity was measured in culture supernatants using a colorimetric detection kit (Roche, Diagostics, Meylan, France).
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bioRxiv - Cancer Biology 2024Quote: DNA was quantified by qPCR using a KAPA library quantification kit (Cat. #KK4835; Kapa Biosystems, Wilmington, Massachusetts, USA). The supplied primer premix was first added to the supplied KAPA SYBR FAST master mix ...
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bioRxiv - Cancer Biology 2024Quote: ... The total RNAs were converted into individually barcoded polyadenylated mRNAseq libraries with the Kapa HyperPrep mRNA kit (Roche). Libraries were barcoded with Unique Dual Indexes (UDI’s ...
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bioRxiv - Cell Biology 2024Quote: Libraries were constructed from 600ng of DNA using the KAPA Hyper PCR-free Library Prep Kit (KAPA Biosystems) on a SciClone NGS instrument (Perkin Elmer) ...
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bioRxiv - Genetics 2024Quote: ... Probes were labeled using the DIG High-Prime DNA Labeling and Detection Starter Kit I (Cat #: 11745832910; Roche). Repeat tract estimation was performed as previously described (Southern blot analysis of repeat expansion size).
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bioRxiv - Genetics 2024Quote: ... Real-time PCR was done using KAPA SYBR FAST qPCR Master Mix (2X) Kit (Kapa Biosystems, Wilmington, MA) on a CFX96 Real-Time PCR Detection System (Bio-Rad ...
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bioRxiv - Genetics 2024Quote: ... kit was used in all qPCR eDNA runs on 96-well reaction plates (Roche Diagnostics GmbH, Mannheim, Germany) with the parameters shown in Fig 3 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... BAC arrayed library filters were screened with a DIG-labeled Pfbra probe (PCR DIG probe synthesis kit, Roche) following the hybridization protocol (DIG high prime DNA labeling and detection starter kit II ...
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bioRxiv - Molecular Biology 2020Quote: Cryosections (10μM) of ventricular tissue were fixed in 4% paraformaldehyde and stained with In-situ Cell Death Detection kit (Roche). Sections were imaged on a laser-scanning confocal microscope (LSM 510 ...
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bioRxiv - Developmental Biology 2021Quote: ... Digoxigenin-labelled antisense and sense RNA probes were synthesized by in vitro transcription using DIG-labelled UTP according to the manufacturer’s instructions (DIG RNA labelling kit, Roche). Primers used were as follow ...
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bioRxiv - Genomics 2020Quote: ... all multiplexed single-cell libraries (n = 30) were quantified using the KAPA Library Quantification Kit for Illumina Platforms (Roche) and pooled in an equimolar ratio ...
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bioRxiv - Developmental Biology 2021Quote: ... Pax3 digoxigenin (DIG) - labeled antisense riboprobes were transcribed from linearized gene-specific probes (PCR DIG probe Synthesis Kit, Roche). WISH experiment was performed as follows ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Molecular Biology 2020Quote: ... RNA-seq libraries were generated using the KAPA HyperPrep Kit with RiboErase (HMR) and KAPA dual-indexed adapters (Roche Sequencing and Life Sciences ...
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bioRxiv - Microbiology 2021Quote: ... Total RNA was extracted by combining phenol-chloroform–isopropanol treatment using the High Pure RNA Isolation Kit (Roche, Germany) (101) ...
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bioRxiv - Genomics 2020Quote: ... The sheared DNA was used for paired-end library preparation with a KAPA LTP Library Preparation Kit (KAPA Biosystems). The optimal numbers of PCR cycles for individual libraries were determined with a Real-Time Library Amplification Kit (KAPA Biosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... followed by quantitation using real-time PCR using the KAPA Library Quant Kit for Illumina (KAPA Biosystems; Cat#KK4835). Libraries were then pooled and sequenced (1×75bp ...
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bioRxiv - Cancer Biology 2020Quote: One microgram of total RNA was reverse transcribed into cDNA using the Transcriptor First Strand cDNA Synthesis Kit (Roche) with the oligonucleotide (dT)18 primer according to the manufacturer’s instructions (30,34) ...
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bioRxiv - Genetics 2020Quote: ... Staining for apoptotic cells was performed using the AP-In situ Cell Death Detection Kit (Roche Diagnostics, Penzberg, Germany) following manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... Total RNA sequencing libraries were prepared using the KAPA RNA HyperPrep Kit with RiboErase for Illumina Platforms (KAPA Biosystems). Briefly ...
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bioRxiv - Genomics 2021Quote: ... RNA-sequencing (RNA-seq) libraries were generated from 300ng RNA using the KAPA RNA HyperPrep Kit with RiboErase (Roche) and sequenced with NovaSeq 6000 (2×150 bp ...
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bioRxiv - Cell Biology 2021Quote: ... A cDNA library was made from Oligo(dT)12–18 as described in the manufacturer’s protocol (Transcriptor First Strand cDNA Synthesis Kit, Roche). Quantitative PCR was performed using Quantifast SYBR Green PCR Kit (204052 ...
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bioRxiv - Genomics 2020Quote: ... Sequencing libraries were prepared using 50 ng of DNA with the KAPA HyperPlus Kit as per the manufacturer’s protocol (Roche). The libraries were sequenced with a paired-end read length of 150bp ...
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bioRxiv - Microbiology 2021Quote: ... The final pool was quantified by qPCR with Kapa Library Quant Kit for Illumina Platforms (KapaBiosystems, Roche, Cat. # KK4824) on an AriaMX Real Time PCR System (Agilent Technologies) ...
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bioRxiv - Microbiology 2021Quote: Total nucleic acid was extracted from clinical isolates using the AMPLICOR® Respiratory Specimen Preparation Kit (Roche, Basel, Switzerland) and purified with 1.8X AMPure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... Samples were further processed using the Kapa LTP Library Preparation Kit for Illumina Platforms (Kapa Biosystems, Part Number KK8232) and indexed with the NEBNext Multiplex Oligos for Illumina 96 Unique Dual Index Primer Pairs (New England Biolabs ...
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bioRxiv - Bioengineering 2020Quote: ... Prepared libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit (Kapa Biosystems / Roche AG, Basel, Switzerland) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Bioengineering 2020Quote: ... Prepared libraries were quantified using KAPA Biosystem’s next-generation sequencing library qPCR kit (Kapa Biosystems / Roche AG, Basel, Switzerland) and run on a Roche LightCycler 480 real-time PCR instrument ...
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bioRxiv - Neuroscience 2021Quote: ... The eaat2a target site was PCR amplified with a fast-cycling polymerase (KAPA2G Fast HotStart PCR kit, KAPA Biosystems) (primers ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized from 400 ng (melanoblasts) or 1µg (cell lines and tissues) input RNA using Transcriptor first-strand cDNA synthesis kit (04897030001, Roche) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... qPCR was performed on a Roche 480 LightCycler using the LightCycler 480 DNA SYBR Green I Master kit (Roche). In all cases ...
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bioRxiv - Neuroscience 2020Quote: ... cDNA synthesis was performed with equal RNA input using the Transcriptor First Strand cDNA synthesis kit (Roche Molecular Biochemicals). Quantitative PCR was performed using C1000 Touch Thermal Cycler (BioRed ...
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bioRxiv - Cancer Biology 2020Quote: ... total RNA was extracted from LOUCY cells using the High Pure RNA Isolation Kit (Roche Life Sciences, Basel, Switzerland) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... NPCs were washed by phosphate buffer saline (PBS) and stained by in situ Cell Death Detection Kit (Roche, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... The complementary DNA (cDNA) was synthesized using a transcriptor high fidelity cDNA synthesis kit (Roche Applied Science, Penzberg, Germany). Each prepared sample used 20 µL of the SYBR green qPCR reaction kit (Roche Applied Science) ...
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bioRxiv - Microbiology 2020Quote: ... 1 μg poly[d(I-C)] and 0.1 μg poly-Lysine (based on DIG Gel Shift Kit, 2nd Generation, Roche) for 15 min at RT ...
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bioRxiv - Microbiology 2020Quote: ... pooled and quantified by qPCR with the KAPA Library quantification kit for Illumina platforms (Kapa Biosystems, Wilmington MA, USA) on a StepOnePlus qPCR system (Thermo-Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was extracted from the culture supernatant after each passage using a High Pure Viral RNA kit (Roche Diagnostics). For deep sequencing of the S1/S2 cleavage site of the viral S gene ...
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bioRxiv - Neuroscience 2021Quote: ... The library amplification step included 6-7 PCR cycles and was performed using the KAPA Library Amplification kit (Roche). The final Hi-C library was purified using SPRIselect beads and the quality and size of the library (approximately 500 bp ...
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bioRxiv - Genetics 2021Quote: BAM files from previously published multi-region WES data (generated using the SeqCapEx Exome Enrichment Kit v3.0 (Nimblegen/Roche)) from nine sporadic fresh-frozen adenomas were available (Supplementary Table 2).11 Somatic variants were re-called using Mutect2 as above.
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bioRxiv - Microbiology 2021Quote: ... The 16S rRNA V3–V4 amplicon was amplified using a KAPA HiFi HotStart ReadyMix PCR Kit (KAPA BioSystems, USA). the amplicon PCR forward primer (5’-CCTACGGGNGGCWGCAG-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... 4 were purified according to the instructions of the manufacturer using the High Pure PCR Template Preparation Kit (Roche) followed by RNAse A digest and a final purification with the High Pure PCR Product Purification Kit (Roche).
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bioRxiv - Plant Biology 2021Quote: ... Library preparation and sequencing were performed by the iGenSeq genotyping and sequencing core facility (ICM Institute - Hôpital Pitié-Salpêtrière AP-HP, Paris, France) using KAPA HyperPrep Kits (Roche, Basel, Switzerland) and the NovaSeq 6000 sequencer (Illumina ...
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bioRxiv - Microbiology 2021Quote: ... hygromycin B phosphotranferase (HYG) or blastacidin S deamidase gene (BSD) generated using the PCR DIG Probe Synthesis Kit (Roche). The blot was developed according to the manufacturer’s instructions.