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Citations for Roche :
3301 - 3350 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2020Quote: ... HPV DNA capture was performed with NimbleGen’s SeqCap EZ Custom Library containing probes for all HR HPV types (Roche NimbleGen, Inc.,) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... For the neuraminidase treatment each well was supplied with 500 μL of a solution containing 50 mU/mL neuraminidase (Roche Diagnostics) in cold PBS+++ pH adjusted to 5.5 ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Cancer Biology 2020Quote: ... All PCR reactions were performed using SYBR-containing master mix from the KAPA Real-Time Library Amplification Kit (Kapa Biosystems #KK2702) and terminated in the mid-exponential phase to limit over-amplification ...
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bioRxiv - Immunology 2021Quote: ... the lamina propria was dissected into small pieces with scissors and incubated in digestion buffer (RPMI 1640 containing 0.17 mg/mL Liberase TL (Roche, Basel, Switzerland) and 30 µg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were washed with ice-cold PBS and scraped into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested from frozen plates by scraping into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001 ...
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bioRxiv - Developmental Biology 2020Quote: An antisense RNA DIG-probe was generated by transcription from linearized pCS1 vector containing Sorbs1 coding sequence using SP6 RNA polymerase kit where UTPs were labelled with digoxigenin (DIG) (Roche, 11175025910).
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bioRxiv - Cell Biology 2022Quote: Murine cells or aortic tissue were lysed in RIPA buffer containing complete protease inhibitor and phosphoSTOP phosphatase cocktails (Roche Applied Science). Lysate was centrifuged at 10,000 g for 10 min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... pellets were dissolved with 300 µl 1X PMPG buffer (1x PBS no Mg2+/Ca2+, 2% Empigen) containing protease inhibitors (Roche, 11873580001) and RNasin (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were scraped on ice in 500 µL of ice-cold PBS containing cOmplete™ Protease Inhibitor Cocktail EDTA-free (Roche) and Phosphatase Inhibitor Cocktails 1 & 2 (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were scraped on ice in 500 µL of ice-cold PBS containing cOmplete™ Protease Inhibitor Cocktail EDTA-free (Roche) and PhosStop (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Immunology 2021Quote: ... Pancreas and liver tissue extract were prepared in RIPA buffer containing a mixture of protease inhibitors (Complete protease inhibitor, Roche Diagnostics).
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bioRxiv - Immunology 2021Quote: ... We resuspended the glomerular fraction in 1mL HBSS buffer with Ca2+ and Mg2+ (Hyclone, Logan, UT) containing 0.1-0.2 mg/mL Liberase™Research Grade (Roche, Mannheim, Germany), and incubated glomeruli in 24-well tissue culture plates at 37°C for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were homogenised in HB buffer (20 mM NaP pH 7.4, 30 mM NaCl containing phosphatase inhibitors (PierceTM) and protease inhibitors (Complete, Roche Diagnostics GmbH)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by perfusion with Hanks’ balanced salt solution with Mg2+ and Ca2+ containing 0.1 mg/mL of Liberase (Roche Applied Science). Following the removal of the liver ...
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bioRxiv - Neuroscience 2020Quote: ... Brain samples were thawed at room temperature and ground in ice-cold Tris-buffered saline (TBS) containing a Protease Inhibitor Cocktail Tablet (Roche, Switzerland), with a final dilution of 1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... phosphate-buffered saline containing 1X complete protease inhibitors (concentration recommended by use of soluble EDTA-free tablets sold by Roche #11873580001) and 10 mM sodium butyrate as a histone deacetylase inhibitor (Sigma # B5887) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl complete LB3 (LB3 containing 1x Roche cOmplete Mini, EDTA-free Protease Inhibitor Cocktail (Roche, #04693159001)) ...
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bioRxiv - Developmental Biology 2020Quote: ... and washed in PBS-PVA to remove proteins from the medium. All samples were lysed in RIPA buffer (cat. #P0013C, Beyotime, CHN) containing protease inhibitor cocktail (cat. #11697498001, Roche, USA). The samples were loaded into 5×SDS loading buffer (cat ...
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bioRxiv - Plant Biology 2020Quote: ... in a total volume of 6 μL containing 0.5 mM of each specific primer and 3 μl of SYBR Green I Master Mix (Roche Applied Science). The second derivative maximum method was used to determine Cp values and PCR efficiencies were determined using LinRegPCR software (http://LinRegPCR.nl) ...
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bioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation and resuspended in 20 mL of lysis buffer (20 mM Tris-HCl, 500 mM NaCl [pH8], containing a protease inhibitor cocktail (Complete®, Roche)) ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed in a final reaction volume of 20 µL containing 10 µL FastStart universal probe master (Roche Diagnostics), 50 ng/µL lambda DNA (New England Biolabs) ...
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bioRxiv - Microbiology 2021Quote: Full-length wild-type human APN encoding plasmid was used as a template to create a glycosilation sequence at position N739 (NWR to NWT change) in a reaction mixture containing: 2X Kapa HiFi (Kapa biosystems) and the following primers ...
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bioRxiv - Neuroscience 2020Quote: ... Beads containing cDNA were then eluted and cDNA was amplified for 21 cycles using Kapa HiFi Hot start ready mix (Roche #07958935001). Amplified cDNA was then purified (GC biotech CPCR-0050 ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Cancer Biology 2021Quote: 1 μl sample from Denaturation was transferred to the qPCR plate well containing the qPCR Mix (Nuclease-free water + KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) + cDNA Primers (from Visium Reagent kit)) ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with ice-cold PBS (w/o calcium and magnesium) and lysed immediately in RIPA-Buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were collected by centrifugation at 3000 x g and resuspended in 1X PBS lysis buffer containing 1mM PMSF and protease inhibitor cocktail (Roche, 5056489001). WCEs were prepared by vortexing with glass beads ...
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bioRxiv - Neuroscience 2022Quote: Mouse hippocampal proteins from PBS control and Aβ oligomer (JD1 positive) treated brain slices were extracted with 2% SDS (in PBS) containing protease and phosphatase inhibitors (Roche, Switzerland) followed by centrifugation at 14000 x g for 15min to precipitate any insoluble material ...
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bioRxiv - Molecular Biology 2023Quote: ... and CBD patients were gently homogenized at 4 °C in 5 mL of TBS buffer containing one tablet of cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) at a concentration of 20% w/vol using a Dounce homogenizer ...
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bioRxiv - Microbiology 2023Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Immunology 2022Quote: ... IL-17RA KO or IL-17A/F DKO mice injected with B16F10-SIY.GFP and EL4-SIY.GFP and disaggregated first mechanically and then enzymatically with 2mL of RPMI containing 2 mg/mL collagenase IV (Roche, cat: 11088882001) and 50 U/mL DNase I (Roche ...
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bioRxiv - Neuroscience 2023Quote: ... Each brain with known weight was homogenized at 4°C using automated homogenizer and with IX RIPA buffer containing protease inhibitor tablet (Roche, Germany). After that the brain lysates were centrifuged at 12,000 rpm for 10 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... the T cells were activated in X-VIVO 15 containing 150 U/mL of human IL-2 (#Ro 23-6019, Roche, Switzerland), 10 ng/mL of recombinant IL-7 (#200-07 ...
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bioRxiv - Biochemistry 2023Quote: ... qPCRs reactions were prepared in a 20 uL final volume containing Fast Start Universal SYBR Green Master (Rox) (Roche Applied Science), cDNA template ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in one microwell of a 96-well plate preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM N5XX primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Genomics 2023Quote: The 19.5 μl eluate was placed in a 0.2 ml PCR tube preloaded with a PCR reaction mixture containing 25 μl of 2x KAPA HiFi HotStart Uracil+ ReadyMix (Roche, KK2801), 1.5 μl of 25 μM P5 transposon primer (ordered from IDT with the sequence shown in Supplementary Fig ...
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bioRxiv - Genomics 2023Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Neuroscience 2023Quote: ... was performed in HB buffer (20 mM Na phosphate pH 7.4, 30 mM NaCl containing phosphatase inhibitors (PierceTM) and protease inhibitors (Complete, Roche Diagnostics GmbH) and nuclei were eliminated by 900 x g centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... The resulting cell pellets were resuspended in Ni column buffer (25 mM Tris HCl, 50 mM NaCl, 30 mM imidazole pH 7.4) containing protease inhibitors (Roche Applied Science). Cells were lysed with 4 passes through an Emulsiflex C3 (Avestin) ...
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bioRxiv - Microbiology 2022Quote: ... incubated overnight at 32°C and replica-plated on a YES agar plate containing 100 mg/L hygromycin B (Roche, #10843555001). The hygromycin plates were incubated for 1-2 days at 32°C until colonies had formed ...
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bioRxiv - Genomics 2022Quote: ... 3 mM MgCl2 and 2 mM CaCl2) containing 0.3 M of sucrose and MNase (30 units of MNase for every 2 million sperm, Roche Nuclease S7) was added to each tube ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were washed and lysed in 2 mL of lysis buffer (containing in mM: 50 Tris pH 7.2, 5 EDTA, 150 NaCl, 10 NEM, Protease Inhibitor Cocktail (Roche, Basel, Switzerland), 2 PMSF ...
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bioRxiv - Biochemistry 2024Quote: ... Quantitative PCR was performed with 2 µL of cDNA as a template in a total volume of 20 µL containing LightCycler FastStart DNA MasterPlus SYBR Green I (Roche, USA) and 0.5 µM primer ...
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bioRxiv - Neuroscience 2023Quote: 30 heads of 8-10 day-old-males per genotype were homogenized in 30 μL Laemmli buffer containing protease inhibitor (Complete mini Protease Inhibitor Cocktail, Roche Diagnostics), using a Minilys apparatus (Bertin Instruments ...
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bioRxiv - Cancer Biology 2024Quote: ... The aqueous phase from each MaXtract tube (∼400 µl) was then transferred to 1.5 ml tubes containing 2 µl of 2 µg/µl glycogen (Roche, Cat# 10901393001), 1 ml ethanol was added ...
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bioRxiv - Microbiology 2024Quote: ... Cells were washed with cold phosphate-buffered saline (PBS) and lysed with NP-40 lysis buffer (P0013F, Beyotime, China) containing protease inhibitor cocktail (04693132001, Roche, Switzerland). Cell lysates were incubated overnight at 4 [with Dynabeads (Sc-2003 ...
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bioRxiv - Cell Biology 2024Quote: The whole lysates of AT or AT-EVs was prepared on ice by using the RIPA lysis buffer (Beyotime, China) containing protease and phosphatase inhibitors (Roche, Germany). After lysis for 20 min ...