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Citations for Roche :
3151 - 3200 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... The cell pellet was resuspended in buffer A (20mM Sodium Phosphate pH 7.4, 300mM NaCl, 30mM Imidazole) containing cOmplete™ protease inhibitor cocktail (Roche) and sonicated ...
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bioRxiv - Cancer Biology 2022Quote: ... Wells were then washed twice with PBT 0.2% and blocked with PBT 0.2% containing 5% bovine serum albumin (Roche, Cat# 10735094001). Primary antibody staining with anti-Wg (1:500 ...
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bioRxiv - Developmental Biology 2022Quote: ... 0.05% Tween 20) before exchange of the pre-incubation solution for hybridization buffer containing 3 ng/µl of digoxigenin-labeled (Roche) riboprobe ...
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bioRxiv - Developmental Biology 2022Quote: ... Matrigel drops (at least 4 samples per time point) were first digested with HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml collagenase type IV (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Whole endothelial extracts were prepared from 70-80% confluent cells in lysis buffer (3% SDS, 60 mM sucrose, 65 mM Tris (pH 6.8) containing protease inhibitor cocktail (Roche-11836170001). Protein extracts were sonicated briefly and the concentration of the isolated proteins was assessed using BCA Protein Assay Reagent (Thermo Scientific™ ...
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bioRxiv - Immunology 2022Quote: ... Single cell suspension was generated by mechanically disrupting tissues through a 100µm filter into FBS followed by a Percoll gradient centrifugation (40% Percoll, GE Healthcare; FBS free media containing 0.5mg/ml DNase, Roche). Cells were then washed with complete media and analyzed by flow cytometry.
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 15 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Tissue was incubated at 37°C for 30 minutes in FBS free DMEM media containing Liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Immunology 2022Quote: ... Cell pellets were lysed by the lysis buffer 10 M urea containing the cOmplete™ protease inhibitor cocktail (Roche, #11697498001). The cell tubes were ultrasonicated at 4 ℃ for two cycle (1 min per cycle ...
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bioRxiv - Genomics 2022Quote: Cells were lysed in RIPA buffer (Beyotime, China) containing protease inhibitors (Complete Mini Ethylene Diamine Tetraacetic Acid-Free Protease Inhibitor Cocktail, Roche). The protein lysates were centrifuged ...
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bioRxiv - Immunology 2022Quote: ... tumor tissue was incubated at 37°C for 30minutes in FBS free DMEM media containing liberase™ TL (0.25mg/ml; Roche) and DNAse I (0.5mg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... washing them twice with PBS containing 1 mM CaCl2 and MgCl2 and collecting cells by scraping them in CaCl2/MgCl2 PBS containing cOmplete protease inhibitor cocktail (Roche). Lysates were cleared by centrifuging at 1000 x g for 10 min at 4 °C and pellets were resuspended in PBS with 1 % Igepal ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Microbiology 2022Quote: ... 33.75 µL cells were mixed with a PCR master mix containing 37.5 µL KAPA HiFi HotStart ReadyMix (Roche, CN KK2602), 2.25 µL second strand synthesis primer SMRT_dT (10 µM ...
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bioRxiv - Molecular Biology 2022Quote: ... Tissue fragments were dissected with sterile scissors in a FBS-free DMEM solution containing 1x Liberase TM Research Grade (Roche) and Antibiotic-Antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2022Quote: Cells were washed twice with PBS and scraped in the according lysis buffer containing 1x cOmplete protease inhibitor cocktail (PI) (Roche) on ice ...
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bioRxiv - Neuroscience 2022Quote: ... eIF2αS/S and eIF2αA/A MEFs transduced with TauRD-YFP and Biosensor cell transfected with wild-type and PS19 brain lysates were lysed with SDS lysis buffer (2% SDS in PBS containing protease and phosphatase inhibitors (11836153001, Roche)) or RIPA buffer ...
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bioRxiv - Molecular Biology 2023Quote: HEK293 tauRD biosensor cell pellets were lysed by resuspension in cold 0.01% Triton in PBS containing cOmplete™ mini EDTA-free protease inhibitor tablet (Roche) at a concentration of 10% w/vol followed by incubation in the lysis buffer for 15 minutes on ice ...
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bioRxiv - Molecular Biology 2023Quote: ... and DS10 cell pellets were lysed by resuspension in cold 0.05% Triton in PBS containing cOmplete™ mini EDTA-free protease inhibitor tablet (Roche) at a concentration of 10% w/vol followed by incubation in the lysis buffer for 20 minutes on ice ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then processed by immobilized metal affinity chromatography (IMAC) on a gravity column containing 2 mL bed volume of cOmplete His Tag purification resin (Roche). After loading the lysate ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM DTT [modified from Cilia Wash Buffer in (Gaertig et al., 2013)] containing the following inhibitors: 1x cOmplete mini EDTA-free protease inhibitors (Roche), 1x PhosSTOP EASY phosphatase inhibitor (Roche ...
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bioRxiv - Immunology 2023Quote: ... and digested using a homogenizer in 500 ml PBS containing 100 mg/ml collagenase/dispase (10-269-638; La Roche). Samples (100 ml each ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) containing protease and phosphatase inhibitors (Pierce Protease Inhibitor Mini Tablet, EDTA-free, Thermo Scientific; PhosSTOP, Roche) for 45 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... the cell monolayers were scraped into 10 ml KPBS (136 mM KCl and 10 mM KH2PO4, pH 7.25, containing protease (Roche, 1836153001) and phosphatase inhibitors (PhosSTOP Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated at 37oC in a staining solution containing nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) for 20-24 hours ...
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bioRxiv - Neuroscience 2023Quote: ... pons and frontal cortex tissues were obtained by homogenization in cold phosphate-buffered saline (PBS) containing protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Biochemistry 2024Quote: ... For lysis the pellets are thawed and resuspended in 2 ml of lysis buffer containing benzonase and cOmplete protease inhibitors (Roche), and processed as described for small scale expression in E ...
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bioRxiv - Physiology 2024Quote: ... the legs of each genotype were homogenized in Radioimmunoprecipitation assay buffer containing a complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland). A Filter Aided Sample Preparation (FASP ...
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bioRxiv - Developmental Biology 2024Quote: ... and incubated for 10 min x2 on a shaker at RT before being transferred to wells containing blocking solution (500 µl horse serum and 500 µl Roche Western Blocking Reagent [both Sigma Aldrich] diluted in 9 ml TNTx) ...
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bioRxiv - Cell Biology 2024Quote: ... lysis buffer (50 mM Tris-HCl pH 7.5, 150 mM NaCl) containing cOmplete EDTA-free protease inhibitor cocktail (Roche #5056489001) and phosphatase inhibitor cocktail (PhosSTOP ...
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bioRxiv - Biochemistry 2024Quote: Treated cells were resuspended in 200 µL of cold DPBS containing protease inhibitor (cOmplete ULTRA tablets mini EDTA-free, Roche, Cat # 05892791001 ...
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bioRxiv - Microbiology 2024Quote: ... Candidate gene fragments inserted into pJC53.2 were amplified by PCR and were used as a template for a transcription reaction containing either Digoxigenin-11-UTP (DIGUTP-RO, Roche) or Fluorescein-12-UTP (11427857910 ...
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bioRxiv - Neuroscience 2024Quote: ... Frozen tissue was transferred to tubes with 1.4 mm stainless steel beads containing 350 ml buffer RLT and homogenized using a MagNA Lyser Rotor (5000 rpm, 10sec, Roche). Samples were further processed according to the respective protocols ...
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bioRxiv - Immunology 2024Quote: Small intestine draining mesenteric lymph nodes (sMLN) and spleens were cut into small pieces and placed in enzymatic digest medium containing 1mg/mL collagenase D (Roche) for 40 minutes at 37°C with shaking at 250rpm ...
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bioRxiv - Genetics 2024Quote: Total protein was isolated from ∼15 larval brains or adult fly heads by homogenization of fly tissue in Radioimmunoprecipitation Assay (RIPA) buffer containing protease inhibitors (Roche). Approximately 20-30µg of total protein was separated using 4-20% SDS-PAGE (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... Total cell lysates were extracted from CAR T cells using radioimmunoprecipitation assay (RIPA) buffer (Thermo) containing protease inhibitor cocktail (Roche). Total protein concentration was determined using Pierce BCA assay (Thermo) ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA containing either the on-target or off-target sites was amplified with KAPA HiFi HotStart DNA polymerase (Roche) or SUN-PCR Blent (SUN GENETICS ...
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bioRxiv - Physiology 2024Quote: 5-10mg of liver tissue was lysed with 400 μl of RIPA buffer (Pierce) containing Complete mini protease inhibitor without EDTA (Roche) and PhosStop phosphatase inhibitors (Roche ...
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bioRxiv - Physiology 2024Quote: ... were transferred in 5ml of NMR Cell Isolation Medium containing 500µl of Cell Dissociation Enzyme Mix: 10mg/ml Collagenase (Roche #11088793001) and 1000Units/ml Hyaluronidase Sigma #H3506 ...
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bioRxiv - Molecular Biology 2024Quote: 5*10^6 cells per condition were harvested after indicated treatments and lysed in RIPA lysis buffer (without SDS) containing cOmplete mini protease inhibitor and PhosSTOP phosphatase inhibitor cocktail (Roche). 2 mg protein from each sample was either incubated with no antibody or rabbit polyclonal anti-RNF20 antibody O/N at 4°C under rotatory agitation ...
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bioRxiv - Molecular Biology 2024Quote: Whole cell lysates were typically obtained by suspending cell pellets or mechanically disrupting mouse tissues in RIPA buffer containing Protease Inhibitor Cocktail (Roche) and 1 mM PMSF (Sigma Aldrich ...
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bioRxiv - Neuroscience 2024Quote: ... the slides were incubated for ∼72 hours at 37°C in staining solution containing nitro blue tetrazolium and 5- bromo-4-chloro-3-indolyl-phosphate (Roche). The slides were finally washed ...
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bioRxiv - Bioengineering 2024Quote: ... lung tissue was minced and incubated in 3 mL of serum-free media containing 0.5 mg/mL DNase I (Roche, 10104159001) and 1 mg/mL collagenase type IV (Worthington ...
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bioRxiv - Neuroscience 2024Quote: ... Lung tissues were cut into small pieces and then digested in RPMI1640 containing 3 mg/mL of collagenase A (Roche) and 0.15 mg/mL of DNase I (Roche ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 15 ng of DNA was amplified in 15 µL reactions containing 7.5 µL of SYBR Green Master mix (Roche, 04887352001) and 0.03 µM of primers [forward ...
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bioRxiv - Biochemistry 2024Quote: IL-2 plasmids were mutated by PCR amplification using primers containing the mutations of interest the KAPA HiFi HotStart ReadyMix PCR kit (Roche) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2024Quote: Mouse tissues were homogenized in the RIPA buffer containing the cOmplete® EDTA-free protease inhibitors (Roche Diagnostics, Mannheim, Germany). After centrifuge at 12,000 rpm for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 x 107 cells were harvested in 500 μL of cold HEPES (10 mM, pH 7.5) containing protease inhibitors cocktail without EDTA (Roche, USA) and phosphatase inhibitors cocktail (Roche ...