Labshake search
Citations for Roche :
3101 - 3150 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: Tumors from MMTV-PyMT mice and C3(1)-Tag mice were resected and minced using a razor blade in DMEM containing 2 mg/mL collagenase and 100 U/mL hyaluronidase (Roche) in a rotator at 37°C for 30 minutes ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: Whole-cell lysates were prepared by using Triton-X sample buffer containing protease inhibitor cocktail from Roche (Grenzach-Wyhlen, Germany). The protein concentration was assessed by using the DC Protein assay reagent obtained from Bio-Rad Laboratories ...
-
bioRxiv - Biochemistry 2020Quote: ... 2.5 mL of Sf9 cells at a density of 1.2×106 cells/mL were transfected with 5 µL of recombinant bacmid containing the gene of interest using 3 µL of X-tremeGENE HP DNA transfection reagent (Roche) and 100 µL of transfection medium (Expression Systems) ...
-
bioRxiv - Immunology 2021Quote: ... the dura mater was carefully removed from the brain and then chopped for enzymatic digestion in Hanks’ Balanced Salt Solution (HBSS) containing 15 mg/mL collagenase D (Roche) and 1 mg/mL DNase (Roche ...
-
bioRxiv - Biochemistry 2020Quote: Cells were washed twice with ice-cold PBS and lysis in ice-cold 100 mM sodium carbonate containing protease inhibitor cocktail (Roche) by tip-probe sonication ...
-
bioRxiv - Biochemistry 2021Quote: The retina was homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Biochemistry 2021Quote: ... retinas were homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Neuroscience 2021Quote: Protein lysates were prepared following euthanasia of zebrafish larvae (6 dpf) in RIPA buffer containing protease inhibitors (Complete ULTRA Tablets, Roche), followed by homogenisation via probe sonication ...
-
bioRxiv - Genomics 2022Quote: ... The reaction was quenched using 125 mM glycine for 5 minutes with rotation and the cells were washed three times using ice-cold PBS containing complete protease inhibitor cocktail tablets (Roche). Cells were resuspended in lysis buffer (1% SDS ...
-
bioRxiv - Developmental Biology 2021Quote: ... The testicular tissue was finely minced and incubated in a dissociating solution containing 40 mg/mL Collagenase H (Roche, Switzerland), 33.3 mg/mL Dispase II (Roche) ...
-
bioRxiv - Cancer Biology 2021Quote: ... Tumors were digested in 500 µL (STAMP microtumors) or 3000 mL (subcutaneous) of phosphate buffered saline containing 0.1 mg/mL DNAse I (Roche, Switzerland) and collagenase D at 1 mg/mL (Roche ...
-
bioRxiv - Developmental Biology 2021Quote: ... 9 and 12h node induced and contralateral uninduced tissues were dissected in ice cold 1x PBS and transferred to low binding PCR tubes containing 1x Protease Inhibitor Cocktail in 1xPBS (cOmplete mini EDTA-free; Roche) on ice ...
-
bioRxiv - Cancer Biology 2021Quote: ... tumors were mechanically minced and incubated for 30-45 minutes in DMEM/Ham’s F12 medium (Fujifilm, 042-30555) containing Liberase DH (Roche, 5401089001) at a final concentration of 0.26 U/mL at 37°C with continuous stirring ...
-
bioRxiv - Cell Biology 2020Quote: ... followed by addition of 300 μL of RIPA buffer containing cOmplete™ protease inhibitor and phosphatase inhibitor (both from Roche). Cells were scraped with cell scrapers and transferred to 1.5 tubes ...
-
bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
-
bioRxiv - Immunology 2021Quote: ... HEK293T and A549 cells were lysed in ice-cold RIPA lysis buffer containing complete protease inhibitor and phosphatase inhibitor cocktails (Roche), resolved by SDS-PAGE ...
-
bioRxiv - Cell Biology 2021Quote: ... The cell pellets were thawed on ice and lysed (3 freeze thaw cycles) after re-suspending each one in 100 μl of PBS containing protease inhibitors (Roche), 0.8 % NP40 ...
-
bioRxiv - Cell Biology 2021Quote: ... whole testes from mice at postnatal day 21(P21) were lysed in RIPA buffer containing protease-inhibitor cocktail (11697498001, Roche). Protein lysates were then blocked by incubation with protein A Dynabeads (10002D ...
-
bioRxiv - Biochemistry 2022Quote: Cells were washed and harvested in PBS 1x, lysed in RIPA buffer 1x (Thermo, 88900) containing phosphatase and protease inhibitors (Roche). Lysates were centrifuged at 15,000 g to separate soluble and pellet fractions ...
-
bioRxiv - Cell Biology 2023Quote: ... CD4+ T-cells were plated in 200ul of medium (RPMI, 10% human serum) containing IL-2 (Roche, 10 IU/mL) and IL-7 (Peprotech ...
-
bioRxiv - Immunology 2022Quote: ... frozen tissue fragments were thawed and incubated for 5 minutes at 37°C in a dissociation buffer containing RPMI-1640 supplemented with 10 U/mL DNase (Roche), 200 U/mL collagenase IV (Worthington Biochemical) ...
-
bioRxiv - Plant Biology 2023Quote: ... untreated and chitosan treated tissue samples were ground in liquid nitrogen and dissolved in an extraction buffer containing Phosstop (Roche), and the concentration of proteins determined by the Bio-Rad protein assay kit with bovine serum albumin (BSA ...
-
bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged for 10 min at 16000 xg and resuspended in 300 µl PBS containing protease inhibitors (cOmplete, Roche). A 16 µl sample was added to 5 µl of 5x SDS sample buffer (300mM Tris-HCl ...
-
bioRxiv - Molecular Biology 2022Quote: ... The dtSARM1 was released from the cytoplasm by incubating with ice-cold PBS containing 200 μM Digitonin and protease inhibitor (Roche).The cleared lysate was incubated with StrepTactinTM resin (IBA ...
-
bioRxiv - Neuroscience 2022Quote: ... Pulverized cortex and hippocampal samples were homogenized on ice using a pestle pellet motor in 10X volume of homogenization buffer containing PhosphoSTOP (Roche) and cOmplete Mini Protease Inhibitor Cocktail (Roche ...
-
bioRxiv - Molecular Biology 2022Quote: ... cell pellets were resuspended in 400 µL Equilibration Buffer A containing protease-inhibitor mix (Complete EDTA-free; 11 873 580 001, Roche). Cells were lysed using the equivalent of 100 µL glass beads and 4 cycles of 30-second on/off vortexing (incubating on ice during the “off” cycle) ...
-
bioRxiv - Molecular Biology 2022Quote: ... cells were collected by centrifugation and re-suspended in Binding Buffer (PBS containing: 2 x cOmplete EDTA-free protease inhibitor cocktail [Roche] ...
-
bioRxiv - Cancer Biology 2024Quote: ... human 293FT cells were grown on 10cm dishes and transfected with sgRNA-containing vector (pX330A-1x2-cNAT10/PITCh) and donor vector using Xtremegene 9 transfection reagent (Roche). 72 h after transfection ...
-
bioRxiv - Bioengineering 2024Quote: ... the supernatant was discarded, and 5 mL of Hank’s Balanced Salt Solution (Ca-, Mg-) (gibco) buffer containing liberase (Roche, #05401119001) (50 mg/mL ...
-
bioRxiv - Neuroscience 2024Quote: hiPSC-DANs cell pellets were collected in ice-cold PBS and were homogenised in RIPA buffer containing protease (cOmplete EDTA-free, Roche) and phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Molecular Biology 2024Quote: ... Pellets were resuspended in 15 ml of Lysis buffer (50 mM Tris HCl pH 7.5, 500 mM NaCl) containing Complete EDTA-free protease inhibitor (Roche, 11873580001), and sonicated 10 cycles of 15 seconds on ...
-
bioRxiv - Molecular Biology 2024Quote: ... Ovaries were cut into 6-8 pieces and enzymatically digested in L-15 media containing Liberase TM (Roche, Indianapolis, IN) and DNase I (Worthington Biochemicals ...
-
bioRxiv - Cancer Biology 2024Quote: ... Samples were minced by mechanical chopping and incubated in 1xPBS containing 150 mg/mL DNAse I (Roche Diagnostics, Indianapolis, IN) and 25 mg/mL liberase (Roche Diagnostics ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We did PCRs in a reaction volume of 25 μl containing 12.5 μl 2x FastStart PCR master mix (Roche, Germany), one forward and two reverse MHC IIB primers (Bracamonte et al. ...
-
bioRxiv - Cell Biology 2024Quote: ... 100 synchronized young adult worms (24h post-L4 stage) were picked into 30 μl of 1XTE (Tris-EDTA Buffer, pH=8) containing 1X complete protease inhibitor cocktail (Roche) and flash-frozen in liquid nitrogen ...
-
bioRxiv - Immunology 2023Quote: ... Hemolymph was extracted by proboscis clipping from groups of 40 mosquitoes per genotype in sterile 1x phosphate-buffered saline (PBS) containing a protease inhibitor cocktail (Roche), and protein concentration determined using Bradford protein quantification assay (Fermentas) ...
-
bioRxiv - Immunology 2024Quote: ... All lung lobes were cut into small pieces and digested in R5 media (RPMI 1640 supplemented with FCS (5 %) containing DNase I grade II (30 µg/ml) and Liberase TM (50 µg/ml, Roche) for 45 min at 37 °C with a magnetic stirrer.
-
bioRxiv - Neuroscience 2023Quote: Frozen brain tissues from human and animals were used to prepare 10% (w/v) homogenates in RIPA buffer containing PI and PhosStop (Roche). Briefly ...
-
Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
-
bioRxiv - Neuroscience 2023Quote: ... Conditioned media was collected into cooled tubes on ice (already containing 20× pre-dissolved protease inhibitor cocktail (PI; cOmplete EDTA-free, Roche) and subsequently centrifuged at 500×g and 5,000×g for 5 min at 4°C ...
-
bioRxiv - Physiology 2023Quote: ... The signal was then visualized by incubating the slides for 48 hours in a solution containing 0.33 mg/mL NBT (Nitro-blue tetrazolium chloride, Roche, 11383213001) and 0.165 mg/mL BCIP (5-Bromo-4-chloro-3-indolyl phosphate ...
-
bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
-
bioRxiv - Neuroscience 2023Quote: Protein extracts from DCN and frontal cortex tissues were obtained by homogenization of brain tissue in cold phosphate-buffered saline (PBS) containing protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (PhosSTOP ...
-
bioRxiv - Immunology 2023Quote: ... and lysed in RIPA buffer (CST; cat# 9806S) or NP-40 lysis buffer containing PhosSTOP phosphatase inhibitor (Roche; cat# 04906845001) and EDTA-free protease inhibitor (BioShop ...
-
bioRxiv - Neuroscience 2023Quote: ... containing 10mL 1X artificial CSF (pH 7.4) supplemented with 1x cOmplete mini EDTA-free protease inhibitor cocktail tablet (Roche, 11836170001) per 10mL ...
-
bioRxiv - Pathology 2024Quote: ... The proteins were redissolved in 8 M Urea/50 mM TEAB buffer containing protease- and phosphatase inhibitors (EDTA free cOmpleteTM, PhosSTOPTM Roche) and treated 30 minutes at 37 °C with 50 units/100 µg tissue benzonase (Sigma Aldrich ...
-
bioRxiv - Molecular Biology 2024Quote: ... cells were lysed 80 h post seeding in RIPA buffer (Thermo, 89901) containing cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysate were centrifuged at 15000 rpm for 15 min ...
-
bioRxiv - Neuroscience 2023Quote: ... were washed with ice cold PBS and incubated in 100 µL of RIPA buffer containing protease inhibitors (Complete ULTRA tablets, Roche). Cells were gently agitated on orbital shaker for five minutes before being spun down at 18 000g for 15 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... the V4 region of the 16S rRNA gene was amplified with barcode primers containing the index sequences using a KAPA HiFi HotStart Real-time PCR Master Mix (Roche). We monitored PCR product amplification and concentration on a Bio-Rad CFT Connect Real-Time PCR system ...
-
bioRxiv - Genetics 2022Quote: ... with 5mM EDTA to remove waste and epithelial cells then were minced and dissociated in RPMI containing collagenase (100 mg/ml collagenase A; Roche), DNase I (50 μg/ml ...