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Citations for Roche :
3051 - 3100 of 8835 citations for FH1 FH2 domain containing protein 1 FHOD1 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... after fixation in 4% formaldehyde and bleaching (Pearson et al., 2009) using blocking solution containing 10% horse serum and western blot blocking reagent (Roche) (King and Newmark ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed with ice-cold RIPA lysis buffer containing EDTA-free protease inhibitor cocktail and PhosSTOP phosphatase inhibitor (Roche), with DNA-shearing by 21-gauge needle ...
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bioRxiv - Neuroscience 2022Quote: ... and age-matched controls lacking evident tau pathology were gently homogenized at 4°C in 10 mL of TBS buffer containing protease inhibitor cocktails (Roche) using a dounce homogenizer ...
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bioRxiv - Molecular Biology 2022Quote: In vitro transcribed RNA was prepared from linearized plasmid containing the human PTH 3’-UTR (68) using a Biotin RNA Labeling Mix (Roche) and T7 RNA polymerase ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were harvested with a cell scraper and lysed by adding 2 x TNI buffer 8 containing complete Ultra protease inhibitor (Roche) and Halt phosphatase inhibitor (Pierce) ...
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bioRxiv - Biochemistry 2020Quote: ... dried and solubilized in 0.2 ml Hepes (100 mM) buffer containing cOmplete™ mini EDTA-free protease inhibitor cocktail (Roche) (1 tablet/20 ml of buffer solution) ...
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bioRxiv - Bioengineering 2020Quote: ... placing the mucus in a beaker of ice-cold PBS containing a cOmplete protease inhibitor cocktail tablet (Roche, Basel, Switzerland). The crude PIM was centrifuged at 11000 × g at 4 °C for 10 min ...
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bioRxiv - Plant Biology 2020Quote: ... The second index was added to the amplified libraries through a second PCR containing 12.5 μl KAPA HiFi Hotstart ReadyMix (Kapa biosystems), 1.25 μl 5 μM p7 primer (Supplemental Table 3) ...
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bioRxiv - Microbiology 2021Quote: ... The 32P-radiolabeled probe was generated from a plasmid containing the entire HPV16 genome by radiolabeling using a Random Prime labeling kit (Roche).
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bioRxiv - Microbiology 2021Quote: ... each tube contained about 106 of cells in 200 μl of the digestion buffer containing 50 μU of neuraminidase (Roche). The tubes were incubated at 37°C for one hour ...
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bioRxiv - Neuroscience 2020Quote: Equal amounts of protein were collected in co-immunoprecipitation buffer (10 mM HEPES, 140 mM NaCl, 0.5% NP-40, pH = 7.4) containing protease inhibitors (Complete mini, Roche Applied Science) and phosphatase inhibitor (100 mM sodium orthovanadate ...
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bioRxiv - Biochemistry 2020Quote: ... The thorax and the abdomen were then washed with 3 ml of pre-chilled 0.1 M KBP pH 7.4 homogenization buffer (HB) containing 1x protease inhibitor (Roche Complete ULTR). The complex was homogenized in 20 ml of HB using 40 ml glass Dounce homogenizer with a loose B pestle (Wheaton Science ...
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bioRxiv - Neuroscience 2020Quote: Cells of 5 pooled organoids were washed with PBS and then lysed in RIPA buffer containing complete protease and phosphatase inhibitor cocktail (Roche). Lysates were cleared by centrifugation at 20.000g for 10min and quantified with the Pierce™ BCA Protein Assay kit (Thermo Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: Total cellular protein was isolated from exponentially growing cells and lysed in ice cold RIPA buffer containing 1x cOmplete Mini Protease Inhibitor Tablets (Roche). Protein concentration were quantified and 75 ug resolved (SDS-PAGE ...
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bioRxiv - Neuroscience 2020Quote: ... frozen tissue was mechanically homogenized in TES buffer (4% Tris Base pH 7.5, 0.1 mM EDTA, 100 mM Na2Cl) containing protease inhibitor (PI) (cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche, Mannheim, Germany)) with the VDI 12 homogenisator (VWR ...
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bioRxiv - Plant Biology 2020Quote: ... with added Roche cOmplete protease inhibitor (# 11836170001, Roche. Samples were further diluted 21 times in 5x Laemmli loading buffer containing Roche protease inhibitor ...
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bioRxiv - Cell Biology 2021Quote: ... Approximately eight million cells were harvested for each sample and lysed into Pierce lysis buffer (Thermo) containing Complete protease inhibitors (Roche). Lysate was clarified by centrifugation at 10,000 g for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... At 80% confluency the cells were harvested on ice by scraping and washed once with PBS containing protease inhibitors (Roche). The cells were pelleted at 300g for 5 min at 4°C and were washed once with KPBS buffer (136 mM KCL ...
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bioRxiv - Plant Biology 2021Quote: ... qPCR reactions were performed in a 10-μl volume containing 5 μl of LightCycler 480 SYBR Green I Master (Roche), 4 μl of the corresponding primer pair (1.5 μM each) ...
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bioRxiv - Microbiology 2021Quote: ... the remaining dermis was washed in Ca2+ and Mg2+ free PBS 5 times and incubated in a digestion buffer containing 2 mg/ml collagenase A (Roche), 100 µg/ml of DNase I (Sigma ...
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bioRxiv - Genetics 2020Quote: ... These pieces were then added to the 3-4 ml of digestion media containing 0.1 mg/ml liberase (Roche, 501003280), 100 U/ml DNase I (Sigma ...
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bioRxiv - Immunology 2021Quote: ... Lungs were cut into small pieces and digested in 5 mL of RPMI containing DNase I (100 μg/mL, Roche), Dispase II (800 μg /mL ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were briefly washed in ice-cold PBS and lysed in RIPA buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Cancer Biology 2021Quote: 160 μl DMEM containing 10 % FBS were added to the bottom wells of xCELLigence Real-Time Cell Analyzer CIM plate (Roche), as chemo-attractant ...
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bioRxiv - Cell Biology 2021Quote: ... Cells lysed with lysis buffer were incubated in the buffer containing protease and phosphatase inhibitor cocktails (04693159001 and 04906845001, Roche), 1 mM serine protease inhibitor PMSF (phenylmethylsulfonyl fluoride ...
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bioRxiv - Cell Biology 2021Quote: The cell pellet was resuspended in 1 mL of ice-cold PBS containing 0.1% NP-40 supplemented with proteinase inhibitor cocktail (PIC) (cOmplete™, Mini, EDTA-free, Roche). The cell suspension was vortexed for five seconds at maximum speed ...
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bioRxiv - Molecular Biology 2022Quote: ... was run on a 15% polyacrylamide gel containing 7M urea and 10 µg/ml APM and then transferred to positively charged nylon membranes (Roche). Pre-hybridization and hybridization steps were performed with Dig Easy Hyb (Roche) ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... hippocampi from each animal were homogenized in ice-cold Homogenization buffer (2 M sucrose, 500 mM HEPES (pH 7,4)) containing complete Protease Inhibitor (Roche/Sigma) and spun down for 10 min at 1000 x g at 4 °C ...
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bioRxiv - Physiology 2022Quote: Mouse platelets were isolated using modified standard procedures,[50] as previously outlined.[44] Washed platelets were resuspended directly in NP-40 lysis buffer containing Complete protease inhibitors (Roche). Glomeruli were isolated using magnetic bead perfusion as previously described,[48 ...
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bioRxiv - Neuroscience 2022Quote: Flash frozen striatal samples were homogenized in a RIPA buffer (Pierce; 89900) containing freshly added phosphatase (Pierce) and protease (Roche) inhibitors ...
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bioRxiv - Cancer Biology 2022Quote: ... Large scale preps of bacteria cultures containing the sgRNA plasmid library were harvested using the Genopure plasmid maxi kit (Roche). SIDP library was packaged in lentiviral particles by large scale co-transfection of HEK293T cells with CELLECTA ready-to-use packaging plasmid (Cellecta – cat.no CPCP-K2A ...
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bioRxiv - Developmental Biology 2022Quote: ... and prepared for flow cytometry immunostaining as follows: Matrigel-embedded organoids were digested using a HBSS solution containing 10 U/ml dispase II (Roche) and 125 U/ml of collagenase type IV (Gibco ...
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bioRxiv - Neuroscience 2022Quote: ... The cortices were immediately removed from the skull and homogenized in RIPA buffer containing protease inhibitors (Roche cOmplete Ref. 11697498001). Homogenates were cleared by centrifugation at 500 x g twice for 7 min ...
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bioRxiv - Microbiology 2022Quote: ... UK) at 1kbar (10g of cells in 40ml final of 20mM Tris pH 8.0 containing protease inhibitor (Roche, EDTA free), at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed once with room temperature PBS and then lysed in a solution of 1 volume 5x SDS-PAGE sample buffer (containing fresh 5% (v/v) beta-mercaptoethanol) to 4 volumes of Immunoprecipitation buffer (IPB; (Thomas et al. 2012)) containing fresh Protease Inhibitor Cocktail (Roche) and 1 μM microcystin-LR ...
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bioRxiv - Immunology 2020Quote: ... Labelled cells were washed twice with 1mL FACS buffer and resuspended in 500uL FACS buffer containing DNAse I (10ug/mL, Roche). Sorting of the cells was performed into 500uL RNAlater on FACSAria III (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... cells were washed with cold phosphate buffered saline (PBS) then scraped into cold PBS containing protease inhibitors (Roche, Laval, QC) and 5 mM sodium fluoride (New England Biolabs ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were dissociated into a single-cell suspension by incubating the retinas in 1mL of HBSS containing 10U of papain (Roche) and 200µM cysteine ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue was thawed on ice and homogenized using a motorized pestle in RIPA buffer containing both protease and phosphatase inhibitor cocktails (Roche). Lysis was completed for 15 minutes on ice ...
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bioRxiv - Biophysics 2021Quote: ... Cells were washed with ice-cold PBS and resuspended in Tris-HCl containing 4mM EDTA and 1X Complete protease inhibitor cocktail (Roche). Every step was then performed at 4° C ...
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bioRxiv - Neuroscience 2021Quote: ... were either left empty or filled with 6 μl of a mix of post-translational modification (PTM) inhibitors containing the following reagents: phosphatase inhibitors (PhosSTOP, 04906845001, Roche), protease inhibitors (cOmplete Protease Inhibitor Cocktail ...
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bioRxiv - Developmental Biology 2022Quote: ... The liver was dissected from the abdominal cavity and placed into a solution containing Hanks’ buffered saline solution (HBSS) supplemented with 1.07 Wünsch units/ml Liberase DH (Roche Applied Science) and 70 U/ml hyaluronidase (Sigma-Aldrich) ...
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bioRxiv - Genomics 2022Quote: Human embryonic hindlimb muscles were collected in sterile PBS and mechanically dissociated using fine scissors in 10 mL (per gram of tissue) of enzyme solution containing 2.5 U/mL Dispase II (Roche, #4942078001) and 1 mg/mL Collagenase B (Roche ...
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bioRxiv - Immunology 2022Quote: ... Tissue pieces then were transferred to a 50-mL conical tube containing 5 ml of digestion medium #2 (RPMI without phenol red containing 5% of FCS, 25 µg/ml of Liberase (Roche), and 200 µg/ml of DNase I (Sigma)) ...
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bioRxiv - Pathology 2020Quote: ... Total and small RNA (containing microRNA) were extracted from MΦ using the Roche High Pure miRNA Isolation kit (Roche, Milan) or the Quick-RNA MicroPrep from Zymo Research (Irvine ...
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bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were harvested in ice-cold PBS using a cell scraper and lysed by sonication for three times 10 seconds in ice-cold PBS containing Complete protease inhibitors (Roche). Unbroken cells were removed by centrifugation at 500 g for 2 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Hemolymph was collected from cold anesthetized mosquitoes by the proboscis clipping method directly into 1X PBS containing 2X EDTA-free cOmplete Mini Protease Inhibitor Cocktail (Roche). Hemolymph protein concentrations were determined using a modified Bradford assay (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... in a reaction volume of 20 μL containing 10 μL of the LightCycler 480 SYBR Green I Master Mix (Roche), 0.3 μM of each specific forward and reverse primer (Table S9 ...
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bioRxiv - Immunology 2020Quote: ... intestines were further chopped and incubated for 30 min at 37°C in medium containing liberase (20 µg/ml, Roche) and DNAse (400 µg/ml ...
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bioRxiv - Zoology 2020Quote: ... the digoxygenin probe is visualized by replacing the APB is replaced with the same containing 20 µl/ml of a commercial NBT/BCIP stock solution (Roche Diagnostics GmBH ...