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Citations for Roche :
3101 - 3150 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... 5µL SYBR Green I Master mix (5 mL: Roche Diagnostics, Indianapolis, IN) and 2 µL of distilled and deionized H2O ...
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bioRxiv - Immunology 2023Quote: ... 50μM beta-mercaptoethanol and 10 ng/ml recombinant human IL-2 (Roche). Both human and murine CD4+ T cells were cultured for 46 hrs under humidified conditions at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mg/ mL of a protease inhibitor cocktail (Roche Diagnostics, Indianapolis, IN). Total protein concentrations within the cleared lysates were then determined using a detergent-compatible Bradford assay (Pierce Biotechnology ...
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bioRxiv - Biochemistry 2023Quote: ... and applied to a 5 mL cOmplete His-tag purification column (Roche). The column was then washed with ∼100 mL of lysis buffer and bound proteins were eluted in 25 mL of lysis buffer supplemented with 300 mM imidazole ...
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bioRxiv - Microbiology 2023Quote: ... 1 tablet per 500 ml complete EDTA-free protease inhibitor cocktail (Roche)) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The supernatant was applied to 2 mL of cOmplete Ni2+-agarose (Roche) prewashed with Buffer B (20 mM NaPi ...
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bioRxiv - Neuroscience 2023Quote: ... The bill-skin was treated with 2 mg/mL collagenase P (Roche) in Krebs solution for 5 minutes ...
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bioRxiv - Developmental Biology 2023Quote: ... Then the samples were treated with 10 µg/mL Proteinase K (Roche) in PTW for 15 min at RT ...
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bioRxiv - Immunology 2023Quote: ... Lung tissue was digested in complete RPMI with Liberase (70µg/mL; Roche) and Aminoguanidine (10mM ...
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bioRxiv - Cell Biology 2024Quote: ... pH 5.5 and 1 mg/mL pronase (both from Roche Diagnostics, Germany), blocked with 5% bovine serum albumin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: Mouse DRG tissues were dissociated with 1 mg/ml Collagenase/Dispase (Roche) in a shaking incubator for 90 min ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 mg/ml lysozyme and protease inhibitor (cOmplete, Mini, EDTA-free, Roche). Lysis was performed by 3 passages through a cell disruptor ...
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bioRxiv - Immunology 2023Quote: ... in a medium containing 125 µg/ml of Liberase (Roche; Meylan, France) and 100 μg/ml of DNase type I (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... pH 7.5) supplemented with 1 mg/ml lysozyme and protease inhibitor (Roche). The cells were lysed by sonication on ice (MSE Soniprep 150) ...
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bioRxiv - Bioengineering 2023Quote: ... LNs were digested with 1 mg/mL Ca2+ supplemented Collagenase D (Roche) for 30 min at 37°C and mashed into a single cell suspension.
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bioRxiv - Genomics 2023Quote: ... and 0.08U KAPA2G Robust HotStart DNA Polymerase (5 U/mL, Roche KK5517). PCR reactions were performed using a thermocycler with the following conditions ...
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bioRxiv - Microbiology 2024Quote: ... 0.2 mM Mg2+-ADP and ∼250 U mL-1 DNase I (Roche), and subsequently lysed in a pre-cooled (4°C ...
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bioRxiv - Immunology 2024Quote: ... and 2 mM L-glutamine (all from Gibco)] supplemented with recombinant human IL-2 (50 U/mL; Roche, cat. 10799068001).
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bioRxiv - Biophysics 2024Quote: ... heart tissue was digested further in 15 mg/ml Collagenase/Dispase (Roche) in Leibovitz medium with 20 mM BDM to create a single-cell suspension ...
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bioRxiv - Cancer Biology 2024Quote: ... then enzymatically dissociated in a 0.1% DNase and Liberase 400μg/mL (Roche/Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: Mammary glands were harvested and digested with collagenase (Roche, 57981821, 3mg/ml) and hyaluronidase (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... and incubated overnight with 0.2 mg/ml of Proteinase K (Roche, 03115801001) at 55 °C ...
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bioRxiv - Biochemistry 2024Quote: ... and 500 µg/ml DNase I (10104159001; Roche Diagnosis GmbH, Manheim, Germany), and the supernatant was clarified by centrifugation (25,000 g ...
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bioRxiv - Biochemistry 2024Quote: ... the 2 ml Ni-NTA resin (cOmplete His-tag purification resin, Roche) was washed with 10 column volume (CV ...
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bioRxiv - Neuroscience 2024Quote: ... the digestion was stopped by adding 0.5 mg/mL DNase I (Roche) in microglia medium (DMEM (PAN Biotech) ...
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bioRxiv - Microbiology 2024Quote: ... and the supernatant was treated with 1 μg/mL DNase I (Roche) for 2 h at 37 °C before 0.22 μm filtration ...
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bioRxiv - Microbiology 2024Quote: ... 10 µg/ml TLCK,and cOmpleteTM EDTA-free protease inhibitor cocktail (Roche)) and added to the lysates ...
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bioRxiv - Microbiology 2024Quote: ... membrane was hybridized with 75 mU/ml Anti-Digoxigenin-AP (#11093274910, Roche) in blocking solution for 60 min at 37°C ...
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bioRxiv - Immunology 2024Quote: ... with 0.17 U/ml of Liberase® (Roche 05-401-020-001), 1 mg/ml of collagenase (Sigma-Aldrich 11088866001 ...
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bioRxiv - Microbiology 2024Quote: ... and 1 tablet per 50 ml complete EDTA-free protease inhibitors (Roche). The cells were subsequently disrupted using an Emulsiflex-C5 high-pressure homogenizer (Avestin ...
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bioRxiv - Developmental Biology 2024Quote: ... and 175 µg/mL 5-bromo-4-chloro-3’-indolyl-phosphate (Roche). Embryos were cleared in 70% glycerol overnight and imaged using Leica M205FA microscope ...
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bioRxiv - Immunology 2024Quote: ... Lungs were digested in DMEM containing 200μg/ml DNase I (Roche, 10104159001), 25μg/ml Liberase TM (Roche ...
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bioRxiv - Molecular Biology 2019Quote: ... mixed-stage transgenic worms expressing CDE-1::GFP were resuspended in equal volumes of 2× lysis buffer (50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, 1% Triton X-100, Roche®cOmplete EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Cell Biology 2020Quote: ... and the pellet resuspended in 100 μl of ice-cold RIPA lysis buffer containing 1x protease inhibitor cocktail (Roche), incubated for 30 min on ice ...
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bioRxiv - Neuroscience 2020Quote: ... the resulting crude synaptosome fraction was then resuspended in IP buffer (50 mM Tris pH 7,4; 100 mM NaCl; 1 mM EDTA, 1% Triton-X; supplemented with Complete Mini protease inhibitors, Roche) and cleared by 3x centrifugation at 20000 g ...
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bioRxiv - Microbiology 2019Quote: ... beads containing bound methylated DNA were resuspended in 250 μl digestion buffer (50 mM Tris pH 8, 10 mM EDTA, 0.5% SDS) with 100 μg Proteinase K (Roche) and incubated at 65°C for 4 h ...
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bioRxiv - Evolutionary Biology 2020Quote: ... tissue was incubated with POD (horseradish peroxidase) conjugated anti-DIG or anti-FLR secondary antibodies (1:100 dilution, Roche Diagnostics ...
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bioRxiv - Microbiology 2021Quote: ... Cells were resuspended in TBSI (50 mM Tris, 100 mM NaCl, pH 8, plus Complete Protease Inhibitor Cocktail, Roche) and broken using 0.1 mm silica spheres (Lysing Matrix B ...
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bioRxiv - Developmental Biology 2020Quote: ... Column was washed three times with wash buffer (PBS 2X, Triton X-100 0.1%, imidazole 50 mM, NaCl 500 mM, DTT 1 mM, 1x Complete protease inhibitor [Roche]) before storage in 50% glycerol (0.01 % Triton) ...
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bioRxiv - Microbiology 2022Quote: Aag2 or U4.4 cells were plated in 48-well plates and transfected with 100 ng of dsRNA per well using 0.4 µL of X-tremeGENE HD (Roche). The medium was refreshed after 3 h and cells were incubated for 48 h ...
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bioRxiv - Developmental Biology 2022Quote: ... cryosections were permeabilized in a sodium citrate solution (0.1% sodium citrate and 0.1% Triton X-100 in 1x PBS) and labelled according to the manufacturer’s protocol (Roche, 11684795910). Afterwards ...
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bioRxiv - Genomics 2019Quote: ... 0.1% Triton X-100) supplemented with 1x protease inhibitors (cOmplete Mini, EDTA-free Protease Inhibitor Cocktail, Roche, CO, #4693159001) and 1x phosphatase inhibitors (PhosSTOP #4906837001 ...
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bioRxiv - Cell Biology 2020Quote: ... 1mM EGTA, 1mM EDTA, 1% Triton X-100, 0,27M sucrose, 1mM DTT, cOmplete EDTA-free protease inhibitor cocktail – Roche, 1mM NaF ...
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bioRxiv - Molecular Biology 2021Quote: ... or NETN Buffer (20 mM TRIS pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% IGEPAL, 1.25 mM DTT and Roche Protease Inhibitor) with several freeze/thaw cycles ...
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bioRxiv - Genomics 2019Quote: We constructed dual indexed libraries using 100-250ng of genomic DNA and the KAPA HTP Library Kit (KAPA Biosystems) on the SciClone NGS instrument (Perkin Elmer) ...
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bioRxiv - Microbiology 2019Quote: ... cell pellets were resuspended in buffer B1 (100 mM Tris pH 7.5, 150 mM NaCl) supplemented with complete EDTA-free protease inhibitor cocktail (Roche), 1 mg/ml lysozyme ...
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bioRxiv - Molecular Biology 2019Quote: Parasite were isolated from rings and early trophozoite stages and lysates were prepared in 1X PBS containing 0.5% TritonX-100 and protease inhibitors (Roche) by sonication (amplitude 30 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell lysates - non-transfected or overexpressing EGFP-tagged proteins -were prepared in lysis buffer (20mM HEPES, 1mM EDTA, 0.2% Triton X-100 and protease inhibitor cocktail (Roche)) briefly sonicated (three times 5 second pulses with 30 seconds rest ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were lysed in 5 pellet volumes of RIPA buffer (25 mM Tris, 150 mM NaCl, 1 % Triton X-100, 0.5 % Sodium deoxycholate, 0.1 % SDS, pH 7.5, 1x Roche Protease Inhibitors) supplemented with 50 units BaseMuncher (Expedeon ...
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bioRxiv - Cell Biology 2019Quote: ... were collected by flash freezing and homogenized in immunoprecipitation buffer (20mM Tris pH7.5, 110mM NaCl, 0.5 % Triton X-100, and protease inhibitors (Roche #11836170001)) with a Dounce homogenizer ...