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Citations for Roche :
2901 - 2950 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... A plasmid (25 ng reporter, 100 ng effector) was transfected with 0.4 μl FuGENE HD (Roche, Basel, Switzerland) per well at a cell confluence level of 50-70% ...
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bioRxiv - Molecular Biology 2020Quote: ... and finally for 5 min in a 1:100 dilution of the substrate CSPD or CDP Star (Roche) in buffer 3.
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bioRxiv - Physiology 2020Quote: ... NaCl 150 mM and Triton X-100 150 mM) with the addition of a protease inhibitors cocktail (Roche), and mechanically disrupted through freeze-thaw cycles ...
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bioRxiv - Cancer Biology 2019Quote: ... washed and incubated with G6B antibody diluted 1:100 in Ventana’s DISCOVERY antibody diluent (Roche Cat#760-108) for 60 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... Nuclei were resuspended in wash buffer [(10 mM Tris-HCl pH 7.5, 100 mM NaCl, 1 mM EDTA pH 8.0, protease inhibitor (Roche)] and rotated for 10 min at 4°C ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche). 150 ul of sonicated chromatin was then incubated 4h at 4°C on a rotating wheel with 3 µg of antibody conjugated to 10 µl of Dynabeads ...
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bioRxiv - Molecular Biology 2020Quote: ... pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% glycerol, 0.5% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2019Quote: ... High Salt buffer (50 mM HEPES pH 7.5, 500 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2020Quote: ... and 0.1% [v/v] Triton X-100) with cOmplete EDTA-free Protease Inhibitor Cocktail (Roche, catalog no. 11873580001) and 1 mM phenylmethylsulfonyl fluoride ...
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bioRxiv - Plant Biology 2019Quote: ... cDNA was diluted to 100 ng/µl and was mixed with the SYBR master mix (Kapa Biosystems, USA), and amplification was carried out according to the manufacturer’s protocol in the StepOne Plus machine (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mM MgCl2) supplemented with 0.5% Triton X-100 and 1 pill Complete® protease inhibitor cocktail (Roche). The cell lysates were subjected to two 5 sec pulses of sonication at the lowest settings (Branson Sonifier 250 ...
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bioRxiv - Genetics 2020Quote: ... then resuspended in 600 µl of ChIP lysis buffer (50mM HEPES, pH 7.5, 140mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, one tablet Roche cOmplete mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2019Quote: ... Cells were washed with PBS twice and lysed with lysis Buffer II (10 mM Tris-HCl pH 7.5, 100 mM NaCl, 0.5% NP-40, 1 mM EDTA and EDTA-free protease inhibitors cocktail [Roche]). As a control of protein synthesis inhibition ...
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bioRxiv - Molecular Biology 2020Quote: ... Fixed cells were resuspended in 10 ml ice cold buffer LB1 (50 mM Hepes-KOH pH 7.5, 140 mM NaCl, 1 mM EDTA, 10% Glycerol, 0.50% NP-40, 0.25% Triton X-100, 1x Roche cOmplete Mini ...
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bioRxiv - Molecular Biology 2021Quote: ... Cell pellets were resuspended in Lysis buffer (50 mM Tris pH 8.0, 300 mM NaCl, 2 mM DTT, 0.3 % TritonX-100, Roche Complete protease Inhibitor Cocktail ...
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bioRxiv - Molecular Biology 2021Quote: ... 2 mM MgCl2, 0.5% Triton X-100, 0.25% Nonidet P-40 and 10% glycerol, supplemented with protease inhibitors [Roche]). After a 10 minute incubation on ice ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl pH8.0, 167 mM NaCl, complete protease inhibitor [Roche]). Antibody (2 μg ...
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bioRxiv - Microbiology 2021Quote: ... Pre-capture libraries were prepared from up to 100 ng input DNA using the KAPA Hyperplus kit (Roche) and TruSeq adapters and barcoded primers (Illumina) ...
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bioRxiv - Microbiology 2021Quote: ... 1.1% Triton X-100, 1.2 mM EDTA, 20 mM Tris-HCl PH8.0, 500 mM NaCl, complete protease inhibitor [Roche]), and LiCl (250 mM LiCl ...
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bioRxiv - Immunology 2019Quote: ... Cells were washed twice with PBS before lysis in 0.1ml lysis buffer (1% Triton X-100, 0.1% SDS, PBS, Complete EDTA-free protease inhibitor [Roche]). Total protein concentration of lysates was quantitated using the Pierce BCA assay (cat no 23225 ...
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bioRxiv - Cell Biology 2019Quote: ... 50 mM Tris, pH 7.2, 125 mM NaCl, 2 mM DTT, 0.1% Triton X-100, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Cell Biology 2019Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche). 5xSDS-PAGE loading buffer containing 25 mM dithiothreitol was added to the lysates ...
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bioRxiv - Cell Biology 2019Quote: Whole-cell lysates were obtained by incubating cells in lysis buffer (1% Triton X-100, 50mM HEPES pH 7.4, 140mM NaCl, 1.5mM MgCl2, 1mM EGTA, 10% glycerol; supplemented with Roche protease/phosphatase inhibitor cocktail (Sigma-Aldrich ...
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bioRxiv - Genomics 2019Quote: ... 0.5 mM EGTA,1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 140 mM NaCl, 1x protease inhibitors-Roche) and once with Buffer-C (10 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100, 0.1% deoxycholate Na, 0.1% SDS, 1 mM AEBSF [Euromedex] and cOmplete EDTA-free [Roche]) with glass beads (diameter ...
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bioRxiv - Genomics 2021Quote: Crosslinked MEFs per protocol above from 6-well dish were washed once in 2 mL PBS and collected in 1 mL cold NE1 buffer (20 mM HEPES pH 7.5, 10 mM KCl, 1 mM MgCl2, 1 mM DTT, 0.1% Triton X-100, Roche Protease Inhibitor Cocktail (Millipore (Burlington ...
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bioRxiv - Developmental Biology 2020Quote: ... 1.5% Triton X-100, 1% IGEPAL, 0.5% Sodium Deoxycholate, 0.1% SDS, 1 mM DTT, 1x Complete protease inhibitor [Roche]) for 30 minutes at 4°C under rotation ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting pellets were resuspended in 1 ml of lysis buffer (Tris-Cl pH 8.0 20 mM, NaCl 150 mM, Triton X-100 0.5%, EDTA 0.5 mM, cOmplete mini [Roche] 3X), frozen in N2 and thawed on ice ...
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bioRxiv - Genomics 2021Quote: ... 2.5 μL of 100 μM sub-barcode primer and 25 μL of KAPA HiFi Hotstart master mix (Roche) was added to each well and the plate was thermocycled (95 °C 3 min. ...
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bioRxiv - Genomics 2020Quote: ... followed by cDNA synthesis using 100 ng RNA with the First Strand cDNA synthesis kit (Roche Applied Science) according to manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... 100 ng of sheared chromatin was end-repaired and A-tailed using the Kapa hyper prep kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... o,1% SDS and 5mM DTT) supplemented with a 1:100 dilution of plant protease inhibitor cocktail (Roche) was added to the ground samples and centrifuged 20 minutes at 6,000 x g at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100, 50 mM Tris pH 7.5, 0.1% SDS, 0.5% sodium deoxycholate, and protease inhibitors [Roche 11836170001]). After 20 min incubation on ice ...
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bioRxiv - Microbiology 2023Quote: ... Pre-capture libraries were prepared from up to 100 ng input DNA using the KAPA Hyperplus kit (Roche) and TruSeq adapters and barcoded primers (Illumina) ...
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bioRxiv - Neuroscience 2022Quote: Brain sections were washed three times then permeabilized in TBS with 0.2% Triton-X 100 (TBST; Roche, Switzerland) at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... in Buffer D (20mM HEPES pH 7.9, 150mM KCl, 20% glycerol, 0.2mM EDTA, 0.2% Triton X-100 and 1x complete Roche inhibitor) overnight at 4°C ...
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bioRxiv - Genomics 2024Quote: ... 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% Sodium Deoxycholate, 0.1% SDS, 1× Protease Inhibitor cocktail, Roche) and incubated at 4 °C for 1 hour with rotation ...
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bioRxiv - Cell Biology 2023Quote: DIV 12-14 cortical neurons were scraped from the plate in lysis buffer (PBS pH 7.5, 0.5% v/v Triton X-100 supplemented with protease inhibitors (EDTA-free Complete; Roche), kept on ice for 15 min and then centrifuged at 15,000 x g for 10 min ...
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bioRxiv - Cell Biology 2024Quote: ... in Buffer D (20 mM HEPES pH 7.9, 150 mM KCl, 20% glycerol, 0.2 mM EDTA, 0.2% Triton X-100 and complete Roche inhibitor) overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 100 / 30 ng (immunoprecipitation/input) used for library preparation using KAPA LTP library preparation kit (KAPA Biosystems). Library preparation was stopped prior to PCR amplification ...
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bioRxiv - Developmental Biology 2023Quote: Dissected embryonic brain structures were lysed in RIPA buffer (Tris HCl 1M, pH 7.7; NaCl 5M; EDTA 0.5M; Triton X-100) supplemented with protease inhibitors (Roche) and phosphatase inhibitors (Sigma Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... cells were treated with 10 µM Olaparib-containing medium for the 48 h before being washed with PBS and pre-extracted with pre-extraction buffer (10 mM Tris-HCl, 2.5 mM MgCl2, 0.5% NP-40, 100× Protease inhibitor cocktail (Roche)) for 5 min at 4°C ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A total of 100 ng RNA was subsequently reverse transcribed using the Transcriptor First Strand cDNASynthesis Kit (Roche) at 55 °C for cDNA synthesis ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Triton X-100) complemented with final concentration of 1X EDTA-free Complete protease inhibitor cocktail (34044100, Roche) and 1 mM PMSF (78830 ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were then resuspended in sonication buffer (50 mM Tris-HCl pH 8.1; 10 mm EDTA; 1% Triton-X; 0,1% deoxycholate sodium; 100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) and sonicated 6 times with 2 min cycles (Branson Sonifier 250 ...
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bioRxiv - Molecular Biology 2023Quote: ... and then lysed and harvested in ChIP lysis buffer (50 mM Tris-HCl pH 8.1; 0.5% SDS; 10 mm EDTA;100 mM NaCl, 1mM PMSF, Proteinase inhibitor Roche) by centrifugation for 6 min at 2,000 × g ...
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bioRxiv - Plant Biology 2023Quote: ... 50 grams of Arabidopsis leaves were homogenized for 1 min in 2 volumes of resuspension buffer (100 mM MES, pH 6.4, 3mM EDTA, 0.5 mM MgCl2, 1mM EGTA, protease inhibitors (Roche)) using a Waring Blender ...
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bioRxiv - Molecular Biology 2023Quote: ... 1% Triton X-100) supplemented with 1X complete protease inhibitor cocktail and 1X complete phosphatase inhibitor cocktail (Roche) using a Precellys Evolution homogenizer (Bertin Technologies) ...
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bioRxiv - Genetics 2023Quote: ... 150 mM NaCl, 2.5 mM MgCl2, 250 mM sucrose, 0.05% NP40, 0.5% Triton X-100, 1x Roche-Complete). Samples were cleared by centrifugation at 10000 × g at 4°C for 10 min ...
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bioRxiv - Immunology 2023Quote: ... 100 ng total RNA was reverse transcribed into cDNA using Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany) according to the manufacturer’s instructions ...