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Citations for Roche :
3151 - 3200 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... were lysed in IP buffer (10 mM Tris pH 7.5, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF and Roche complete EDTA free protease inhibitor cocktail) ...
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bioRxiv - Genomics 2021Quote: ... and resuspended in 1 mL Nuclear Isolation Buffer ((20mM HEPES, 10mM KCl, 1mM MgCl2, 0.1% Triton X-100, 20% Glycerol, and 1X Protease Inhibitor (Roche)) per 5-10e6 cells by gently pipetting 5x with a wide-bore tip to release nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... A total of ∼ 8×107 cells were lysed in 2 mL lysis buffer (20 mM Tris HCl pH 7.4, 150 mM NaCl, 1% Triton-X 100 supplemented with protease inhibitor Complete, Roche). Lysates were rotated at 4°C for 20 minutes and subsequently centrifuged at maximum speed for 30 minutes ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl, 5 mM EDTA, 5 mM EGTA, 0.1% Triton X-100, 1 mM PMSF, 1x protease inhibitor cocktail [Roche] ...
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bioRxiv - Molecular Biology 2020Quote: ... and resuspended in proteome lysis buffer (50 mM Tris HCl pH 8.0, 8 M urea, 75 mM NaCl, 100 mM sodium butyrate, cOmplete protease inhibitors – Roche). Cells were then mechanically lysed (MagNAlyzer Roche – 6000 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... at 4 °C for 4 hr and washed two times with its resuspending buffer and once with E100 buffer (25 mM HEPES-KOH pH7.5, 10 % glycerol, 100 mM KCl, 2 mM MgCl2, 1 mM EDTA with Roche cOmplete™ ...
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bioRxiv - Biochemistry 2020Quote: ... Lacrimal glands were also harvested and homogenized in 0.2% Triton X-100 (MilliporeSigma) containing a protease inhibitor cocktail (Roche) and prepared for PRG4 western blotting (2.2) ...
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bioRxiv - Neuroscience 2020Quote: ... The ipsi- and contralateral cortices were immediately transferred to lysis buffer (50 mM Tris, pH 7.6, 150 mM NaCl, 1% Triton X-100, CompleteMini Protease Inhibitor Mixture, Roche). The cortices were homogenized and incubated on ice for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... and resuspended in MBP-lysis buffer (100 mM Tris-HCl pH 7.0, 200 mM NaCl) supplemented with EDTA-free protease inhibitor cocktail (Roche) and lysed using sonication ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Neuroscience 2019Quote: ... which were blocked and permeabilized with 5% normal goat serum in 0.25% Triton X-100 in PBS (Roche Diagnostics) for 30 min at 25°C ...
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bioRxiv - Cell Biology 2021Quote: ... cells were washed with ice-cold 1x PBS added with 1.5 mM Na3VO4 and 50mM NaF before being lysed in lysis buffer (1X PBS, 1% Tx-100, 1.5 mM Na3VO4, 50mM NaF, PhosSTOP (Roche) and cOmplete protease inhibitors (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: Retinas were lysed on ice for 10 min in lysis buffer (20mM HEPES, 150mM NaCl, 1.5mM NgCl2, 2mM EGTA with 0.5% TritonX-100) containing protease inhibitors (Roche #5892970001) and phosphatase inhibitors (10mM NaF ...
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bioRxiv - Neuroscience 2021Quote: Cells were lysed in ice cold 1% Triton lysis buffer (20mM Tris-HCl pH 8.0, 10mM EDTA, 1% Triton X-100 and 1x protease inhibitor cocktail (5892970001, Roche)) and protein concentration was measured using Bio-Rad BSA protein assay (5000116 ...
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bioRxiv - Microbiology 2021Quote: ... saponin lysed late ring-stage parasites that had been cross-linked with 1 mM DSP were solubilised with 0.5% Triton X-100 in 1x PBS supplemented with cOmpleteTM protease inhibitor cocktail (Roche) at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... pH 7.4, 150 mM NaCl, 5 mM EDTA, 5% glycerol, 1% Triton X-100, and 1x complete protease inhibitor [Roche]). Cleared lysates were then incubated with glutathione sepharose (GE healthcare ...
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bioRxiv - Cancer Biology 2020Quote: ... 100-500 ng of DNA were used to prepare libraries using the KAPA Hyper Prep Kit (Kapa Biosystems KK8504) with 8 cycles of PCR ...
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bioRxiv - Cell Biology 2021Quote: ... membranes were removed from neurons cultured in MatTek dishes at 8-10 days in vitro (DIV) by incubating cells with extraction buffer (1M sucrose, 0.15% Triton X-100 (Roche, Basel ...
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bioRxiv - Cell Biology 2021Quote: ... Frozen pellets were thawed into 10 volumes of ice-cold hypotonic lysis buffer (0.1% SDS, 1% Triton X-100, 1x cOmplete protease inhibitor cocktail (Roche)) ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in a 50 mM sodium phosphate buffer at pH 7.8 (buffer A) supplemented with DNase I (100 U, DNase I recombinant, RNase-free Roche), and disrupted using a French Pressure Cell (3 cycles at 1100 psi) ...
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bioRxiv - Molecular Biology 2022Quote: ... in lysis buffer (50 mM Tris–HCl pH 7.5, 300 mM NaCl, 10 mM imidazole, 5 mM β-mercaptoethanol, 0.5% Triton X-100, Roche Complete Mini EDTA-free protease inhibitor tablets (Sigma-Aldrich)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 1 mM protease inhibitor cocktail (1 mM EDTA, 20 mM NaF, 0.5% NP-40, 1% Triton X-100) (Roche) and 0.5% Na3VO4 on ice ...
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bioRxiv - Physiology 2022Quote: ... for 3 min and incubated twice for 3 min with CSK buffer containing 0.5% Triton X-100 and complete protease inhibitors cocktail (catalog no. 11873580001, Roche). Cells were after washed with PBS-S ...
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bioRxiv - Microbiology 2022Quote: ... These samples were then mixed with an equal volume of 2-fold lysis buffer (2% TX-100, 20 mM MES, 30 mM Tris, pH 7.4, 20 mM DTT, 1x protease inhibitor cocktail [PIs, Roche cOmplete] with 0.2 M ...
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bioRxiv - Genomics 2022Quote: ... Beads were washed twice 10 min with cold buffer I (150 mM NaCl, 1% Triton X-100, 0.1% SDS, 2 mM EDTA, protease inhibitor cocktail (Roche)) ...
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bioRxiv - Cancer Biology 2022Quote: Cells were washed in cold PBS and resuspended in 0.5% v/v Triton X-100 in PBS supplemented with a cocktail of protease (Roche) and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... cells were lysed in 100 mM Tris pH 8.0 with 1% SDS supplemented with protease inhibitor (Complete EDTA-free, Roche). Cells in lysis buffer were heated 3 times for 5 min at 95 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 µg of pcDNA 3.1(-) empty vector or C/EBPβ deletion mutants in 100 mm dishes using X-tremeGENE HP (Roche) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... Embryos were then rinsed in methanol followed by PTX (0.3% Triton X-100 in PBS 1x) and incubated in blocking reagent (Roche) for 1 hour at RT ...
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bioRxiv - Biochemistry 2020Quote: ... cells were extracted using NETN buffer (20 mM Tris at pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5% Igepal, 1.25 mM DTT, Roche protease inhibitor) with several freeze/thaw cycles ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% SDS and 1% sodium deoxycholate) supplemented with protease and phosphatase inhibitors (100 mM PMSF and PhosSTOP tablet (Roche)) ...
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bioRxiv - Molecular Biology 2020Quote: ... 1 l E.coli culture was harvested and re-suspended in the lysis buffer (50mM HEPES pH7.5, 150mM NaCl, 5mM MgCl2, 1% Triton X-100, EDTA-free protease inhibitor cocktail (Roche) and 2mM PMSF (Sigma/Merck) ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl) plus 0.5% Triton X-100 and one tablet of cOmplete ™ Protease Inhibitor Cocktail Tablet (Roche).
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bioRxiv - Genomics 2020Quote: ... and resuspended in 1 mL Nuclear Isolation Buffer (20mM HEPES, 10mM KCl, 1mM MgCl2, 0.1% Triton X-100, 20% Glycerol, and 1X Protease Inhibitor (Roche)) per 5-10 e6 cells by gently pipetting 5x with a wide-bore tip to release nuclei ...
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bioRxiv - Biophysics 2020Quote: ... RNC samples were buffer exchanged into deuterated Tico buffer (100% D2O and d8-HEPES, pH 7.5) supplemented with protease inhibitors (Roche), with a final sample concentration of 7.5 μM ...
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bioRxiv - Cell Biology 2021Quote: ... mitochondria were lysed in lysis buffer (50 mM Tris pH 7.4, 100 mM KCl, 1 mM EDTA, 1× Complete Protease Inhibitor cocktail (Roche), 1mM PMSF ...
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bioRxiv - Cell Biology 2021Quote: ... 1% Triton X- 100, 2 mM EDTA, 20 mM Tris, pH 8.1, 150 mM NaCl, and protease inhibitors; Roche), and then washed once with 1 ml of “high-salt buffer” (the same but with 500 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... The frozen retinas were subsequently homogenized by repetitive pipetting on ice in PBS with 0.5% TritonX-100 and cOmplete protease inhibitors (Roche). The homogenates were precleared by incubation with protein G Sepharose (GE Healthcare ...
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bioRxiv - Neuroscience 2022Quote: Whole brain samples were homogenized in carbonate buffer (pH 11.5, 100 mM Na2CO3, 50 mM NaCl, protease inhibitor) using the MagNA Lyser (Roche) for 5 s at 5000 speed ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... pH 8.0) augmented with protease and phosphatase inhibitors (1:100 HALT PI cocktail, Thermofisher Sci Cat# 78440; or Roche cOmplete ULTRA [Cat# 05892970001] plus phosStop [Cat# 4906845001] tablets) ...
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bioRxiv - Biochemistry 2019Quote: ... nuclei were harvested as above and the nuclear pellet resuspended in 100 µl extraction buffer (3mM EDTA, 0.2mM EGTA and protease inhibitors (Roche)) ...
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bioRxiv - Neuroscience 2019Quote: Microdissections were triturated in 100 μl of lysis buffer (150 mM NaCl, 1% Triton, 100 mM Tris, pH 7.4, 10 mM EDTA, 1X protease/phosphatase inhibitor (Roche)) per 10 mg of tissue and incubated 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were washed 3x with PBS and then lysed in 1 ml TX-100 lysis buffer (1% Triton X-100, 150mM NaCl, 50 mM Tris-HCl, pH 7.4) supplemented with protease inhibitor cocktail (Roche). Cell lysates were cleared by centrifugation at 10,000 × g for 10 mins at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... 0.5% NP-40, 0.5% TX-100, 150 mM NaCl plus complete EDTA-free Protease Inhibitor Cocktail Tablets, Roche, #11697498001), diluted 2:1 in 4X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Microbiology 2019Quote: ... were incubated 15 min in lysis buffer (20 mM HEPES buffer, pH 7.0; 100 mM NaCl; 5 mM MgCl2; 1% Triton X100; protease inhibitors (Roche)) and centrifuged for 15 min at 14 000 rpm in a refrigerated microfuge ...
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bioRxiv - Microbiology 2019Quote: ... Transfected cells were washed two times in cold PBS and lysed in lysis buffer (20 mM Hepes, 100 mM NaCl, 5 mM MgCl2, 0,2 % Triton X100, 10 % Glycerol, protease inhibitors (Roche)) ...
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bioRxiv - Genomics 2019Quote: ... were lysed in 100 ul Buffer-B (50 mM Tris-HCl, pH 8.0, 10 mM EDTA, 1%SDS, 1x protease inhibitors -Roche) and sonicated in a microtube (Covaris ...
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bioRxiv - Molecular Biology 2019Quote: ... Beads were washed with Low Salt buffer (50 mM HEPES pH 7.5, 140 mM NaCl, 1% Triton X-100, 1x protease (Roche) and phosphatase inhibitors (Sigma-Aldrich)) ...
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bioRxiv - Developmental Biology 2021Quote: ... is followed by probe titration (100-300 ng per slide dependent on the probe, dissolved in Ribohybe reagent (Roche)) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and lysed by sonication in prenylation buffer (50 mm HEPES, pH 7.2, 50 mm NaCl, 2 mm MgCl2, 100 μm GDP, 1× Roche complete EDTA-free protease inhibitor cocktail) ...