Labshake search
Citations for Roche :
3351 - 3400 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2019Quote: ... 10 mM Na4P2O7 and 10 mM EDTA supplemented with 1% (v/v) Triton X-100 and one complete protease inhibitor cocktail tablet (Roche)) ...
-
bioRxiv - Immunology 2019Quote: ... Lysis buffer was added (40 mM HEPES, 140 mM NaCl, 0.1% Triton-X-100, Roche EDTA-free complete protease inhibitor) and cells were detached with a cell scraper ...
-
bioRxiv - Microbiology 2019Quote: ... resuspended in 1 mL ice-cold lysis buffer (100 mM NaCl, 5% glycerol, 1 mM dithiothreitol and protease inhibitor cocktail (cOmplete, EDTA-free; Roche) in 50 mM Tris HCl ...
-
bioRxiv - Neuroscience 2019Quote: ... Following permeablisation in PBSTx (0.3% Triton 100 X in PBS) EdU labelling was detected by incubating tails in fresh EdU Click-iT reaction solution (Roche) for 2 hours in the dark at room temperature ...
-
bioRxiv - Neuroscience 2019Quote: ... cells were lysed in Hepes buffer (20 mM Hepes, 1% CHAPS, 100 mM NaCl, 5 mM EDTA, protease inhibitor cocktail (Roche), phosphatase inhibitor cocktail III (Sigma)) ...
-
bioRxiv - Microbiology 2019Quote: Whole cell lysates were prepared by suspending purified parasite pellets in whole cell lysis buffer (1XPBS, 1% v/v Triton X-100) containing Protease Inhibitors (cOmplete EDTA-free, Roche). 24 h in vitro ookinetes were also subjected to cellular fractionation using the following method ...
-
bioRxiv - Cell Biology 2019Quote: Cell pellets were washed twice with ice-cold PBS prior to lysis in lysis buffer supplemented with 1% Triton X-100 and protease inhibitors (Roche) on ice ...
-
bioRxiv - Molecular Biology 2020Quote: ... 0.5 mM EGTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-Deoxycholate, 140 mM NaCl, 1x Roche cOmplete protease inhibitors). Per IP 30 μl of Protein G Dynabeads (Invitrogen ...
-
bioRxiv - Cell Biology 2021Quote: ... Pellets were lysed in 300 µL FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 mL ceramic beads on a Fastprep-24 (MP Biomedicals) ...
-
bioRxiv - Microbiology 2021Quote: ... were added (0.5 g to each 100 mg pellet) along with protease inhibitor solution (complete mini EDTA-free protease inhibitor cocktail, Roche), dH2O and 1 mM phenylmethylsulfonyl fluoride (PMSF ...
-
bioRxiv - Molecular Biology 2020Quote: ... The pelleted nuclei were resuspended in 1 ml ice cold shearing buffer (10 mM Tris-HCl pH 8.0, 100 mM NaCl, 1 mM EDTA, 0.5 mM EGTA, 0.1% Na-Deoxycholate, 0.1% SDS, 1x Roche cOmplete protease inhibitors). Buffer compositions for LB1 ...
-
bioRxiv - Developmental Biology 2020Quote: ... of 90-100% confluent HEK293T cells were lysed directly using CERI and NER buffer supplemented with 1x protease inhibitor cocktail (Roche), 1x PhosSTOP (Roche) ...
-
bioRxiv - Neuroscience 2019Quote: ... The bacterial pellet was dissolved in lysis buffer (20 mM Tris pH 8.0, 100 mM NaCl, 1 protease inhibitor tablet (Roche Complete), 0.5 mM EDTA ...
-
bioRxiv - Genetics 2021Quote: ... cells were collected in lysis buffer (TBS + 1% Triton X-100) containing protease inhibitors (Complete Mini EDTA-Free, Roche 04693159001), briefly sonicated ...
-
bioRxiv - Plant Biology 2019Quote: ... qRT-PCR reaction volumes of 100 nL consisted of 1 X LightCycler 480 SYBR Green I Master (Roche Diagnostics France), a cDNA sample of 1 ng.μL−1 ...
-
bioRxiv - Molecular Biology 2019Quote: Batches of ten 2 day-old adult flies were crushed in 100 µl of lysis buffer (0.3% SDS in PBS plus one EDTA-free cOmplete™ Protease Inhibitor Cocktail tablet, Roche), incubated for 15 min and centrifuged at 15,000 gmax for 10 min (all manipulations at room temperature) ...
-
bioRxiv - Genetics 2021Quote: ... 3.2mM MgCl2, 0.25% Triton X-100, 0.25% NP-40, 0.1% Na-deoxycholate and 1mM DTT and Roche Complete protease inhibitor). To this 4ul of Benzonase (E1014 ...
-
bioRxiv - Genetics 2021Quote: ... 3.2mM MgCl2, 0.25% Triton X-100, 0.25% NP-40, 0.1% Na-deoxycholate and 1mM DTT and Roche Complete protease inhibitor), 2ul of Benzonase (E1014 ...
-
bioRxiv - Biochemistry 2020Quote: Cells were washed twice with ice-cold PBS and lysis in ice-cold 100 mM sodium carbonate containing protease inhibitor cocktail (Roche) by tip-probe sonication ...
-
bioRxiv - Cell Biology 2021Quote: Round spermatids enriched by STA-PUT were lysed with 1% Triton X-100 in PBS supplemented with EDTA-free protease inhibitor cocktail (Roche) by gentle rocking at 4°C for 2 hours ...
-
bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Sample quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
-
bioRxiv - Physiology 2020Quote: 100 µL of whole blood was pelleted by rapid centrifugation and pellets were lysed in 100 µL of RIPA lysis buffer with proteinase inhibitor cocktail (Roche). Following an additional centrifugation step ...
-
bioRxiv - Neuroscience 2020Quote: ... and lysed by the addition of 100 μl of ice-cold RIPA lysis buffer (Beyotime) with protease inhibitor cocktail (Roche). Cellular debris was removed by centrifugation at 13,000 g for 15 min at 4°C ...
-
bioRxiv - Biochemistry 2021Quote: ... 10 mM Imidazole pH 8) and supplemented with PMSF (1:100), protease-inhibitor mix HP Plus (1:500, Serva) and DNaseI (1:300, Roche), lysed by sonication and cleared by centrifugation at 108000g for 45 min ...
-
bioRxiv - Biochemistry 2021Quote: The retina was homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Biochemistry 2021Quote: ... retinas were homogenized in lysis buffer (100 mM Tris-HCl [pH 7.4], 300 mM sucrose) containing a 1X cOmplete protease inhibitor cocktail (Roche, Switzerland). After removing the tissue debris (800 × g ...
-
bioRxiv - Microbiology 2021Quote: ... before being resuspended in 1 mL of lysis buffer (100 mM NaCl, 5% glycerol, 10 mM Tris, pH 8; one cOmplete Mini EDTA-free protease inhibitor pellet (Roche) per 15 mL) ...
-
bioRxiv - Microbiology 2021Quote: ... the samples were washed with cold 1x PBS three times and before scrapping the samples in cold lysis buffer (50 mM Tris pH 7.6, 200 mM NaCl, 1% Triton X-100, 0.5% CHAPS + complete protease inhibitor/Roche Cat# 11836153001). The samples were mechanically disrupted by passing them through a 27.5 syringe five times before sonication on ice (1 sec on ...
-
bioRxiv - Neuroscience 2021Quote: ... tissue was homogenized by probe sonication at 20kHz for 10s in ice-cold TBS buffer (tris-buffered saline, 1% Triton X-100; pH 7.4) containing protease inhibitor (Roche 11697498001) and phosphatase inhibitor (Sigma 4906845001 ...
-
bioRxiv - Genetics 2021Quote: Animals were resuspended in lysis buffer (50 mM Tris/HCl pH 7.4, 100 mM NaCl, 1% v/v SDS, complete protease inhibitor cocktail (Roche Diagnostics)) ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... 0.1% SDS and 1%Triton X-100 and protease inhibitors cocktail (Mini-Complete EDTA-free; Roche Applied Science, Penzberg, Germany). Lysates were centrifuged (13000g ...
-
bioRxiv - Neuroscience 2021Quote: Dissected brain regions of interest or culture samples were homogenized with TX-soluble buffer (50 mM Tris [pH 8.0], 150 mM NaCl, 1% Triton-X 100) containing protease and phosphatase inhibitors (Roche, USA). The supernatants were collected for soluble fraction after centrifugation (20 ...
-
bioRxiv - Microbiology 2020Quote: ... Plasmids were digested in 50-100 μl total volumes with restriction enzymes and their appropriate buffer (ether Roche or NEB) in accordance with the manufacturers recommendations (typically 1 μg of DNA was digested with 1 unit of enzyme for 1 hour at 37°C) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Coverslips were immediately moved to ice cold cytoskeletal buffer (10mM PIPES pH 6.8, 300mM sucrose, 50mM NaCl, 3mM EDTA, 0.5% Triton X-100, Protease Inhibitor Cocktail [cOmplete™ EDTA-free; Roche, 27368400]) for 5 minutes before fixation in 2% PFA ...
-
bioRxiv - Cell Biology 2021Quote: HEK293T cells transfected with sfGFP-TAOK2 WT and sfGFP-TAOK2 K57A were lysed with HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, Complete)) ...
-
bioRxiv - Cell Biology 2021Quote: ... and lysed in HKT buffer (25mM HEPES pH7.2, 150mM KCl, 1% Triton X-100, 1mM DTT, 1 mM EDTA, Protease Inhibitors (Roche, Complete), Halt Phosphatase Inhibitors (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2021Quote: ... The cell pellets were thawed on ice and lysed (3 freeze thaw cycles) after re-suspending each one in 100 μl of PBS containing protease inhibitors (Roche), 0.8 % NP40 ...
-
bioRxiv - Microbiology 2020Quote: ... The cells were then washed with PBS and lysed in HEPES buffer supplemented 100 μM N-ethylmaleimide and protease inhibitor cocktail (Roche). The lysates were centrifuged and incubated with 2 μg Mcl-1 antibody (S-19 ...
-
bioRxiv - Cancer Biology 2020Quote: ... 50 mM NaCl, 50 mM sodium pyrophosphate, 50 mM sodium fluoride, 1% Triton-X-100, 10% glycerol, 5 mM EDTA, Roche MiniProtease Inhibitor cocktail tablet ...
-
bioRxiv - Neuroscience 2022Quote: ... and proteins were transferred to an activated (100% ethanol, 1 min; followed by two washing steps with water) PVDF membrane (Roche Diagnostics GmbH ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 1x Roche cOmplete Protease inhibitors), 1x 1 ml LiCL buffer (250 mM LiCl ...
-
bioRxiv - Developmental Biology 2022Quote: ... 10 mM Tris-HCl pH 8.0, 1 mM EDTA, 1% Triton X-100, 0.1% SDS, 0.1% Na-deoxycholate, 1x Roche cOmplete Protease inhibitors) in a total volume of 900 μl ...
-
bioRxiv - Molecular Biology 2022Quote: Pellets were thawed and lysed in 300 µl FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 ml ceramic beads on a Fastprep-24 (MP Biomedicals) ...
-
bioRxiv - Cancer Biology 2022Quote: 100 to 500 ng of total RNAs were reverse-transcribed with Transcriptor First Strand cDNA synthesis kit (Roche, Basal, Switzerland). qPCR experiments were performed using the StepOnePlus qPCR system (Applied Biosystems ...
-
bioRxiv - Biophysics 2022Quote: ... cells were gently washed twice with 500 μl cold PBS and directly lysed in 50 μl of lysis buffer (PBS, 1% (v/v) Triton X-100 and protease inhibitor cocktail (Roche)) ...
-
bioRxiv - Genetics 2022Quote: ... COS-7 cells transiently expressing POPDC constructs with the appropriate epitope tag were lysed using a 1% (v/v) Triton X-100 based lysis buffer supplemented with cOmplete protease inhibitor cocktail (Roche). Lysates were subjected to Co-IP using the ProFoundTM c-Myc Tag IP/co-IP Kit (Thermo Scientific ...
-
bioRxiv - Genomics 2022Quote: ... RNA Libraries were prepared from 100 ng of total RNA with the KAPA Stranded mRNA-Seq Kit (Kapa Biosystems KK8401). Indexed libraries were then pooled and 2×50 bp ...
-
bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 20% glycerol, 1% Triton X-100, and 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma) ...
-
bioRxiv - Plant Biology 2022Quote: ... 150 mM NaCl, 1 mM EDTA, 10% glycerol, 1% Triton X-100, 1 mM PMSF, 1x cOmplete protease inhibitor cocktail from Roche, 1x phosphatase inhibitor cocktail 2 and 3 from Sigma ...
-
bioRxiv - Plant Biology 2022Quote: ... total proteins were extracted with lysis buffer (10 mM Tris-Cl pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 0.5% NP-40, 100 mM PMSF, and 1X Roche protease inhibitor cocktail), followed by incubated with anti-GFP beads (AB_2631358 ...