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Citations for Roche :
3301 - 3350 of 5210 citations for Phthalic Acid Unlabeled 100 Ug Ml In Mtbe since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: Cell pellets of 1 × 106 cells were resuspended in 100 μl RIPA lysis buffer containing a protease inhibitor cocktail (Roche). Samples were centrifuged for 5 min to remove cell debris and stored at −20°C until use for Western blot analysis.
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bioRxiv - Evolutionary Biology 2021Quote: ... Chromo-1 transcriptome sequencing was performed on the Illumina HiSeq platform (UCLA Clinical Microarray Core) with read lengths of 100 bp using the KAPA stranded RNA-seq kit (Roche) to construct paired-end libraries ...
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bioRxiv - Developmental Biology 2020Quote: Cells were lysed in TNT buffer (1% Triton X-100, 150 mM NaCl, 50 mM Tris HCl, pH 8) with protease inhibitors (Roche). 5 uL of whole cell lysate (approximately 40 μg ...
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bioRxiv - Developmental Biology 2020Quote: ... fixed embryos were homogenized in 2-5 ml freshly prepared lysis buffer (50 mM Tris pH7.5; 150 mM NaCl; 5 mM EDTA; 0.5 % NP-40; 1.15 % Triton X-100; 1x Roche Complete protease inhibitors) with a Dounce Homogenizer on ice ...
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bioRxiv - Cell Biology 2022Quote: ... and lysed on ice for 15 minutes with 100 µl lysis buffer (50 mM HEPES, 150 mM NaCl, 1% TritonX100) supplemented with protease and phosphatase inhibitor cocktail (Roche). 10% of the soluble fraction of the lysate was boiled with 4X sample buffer ...
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bioRxiv - Neuroscience 2021Quote: ... 0.1% Triton X-100, pH 7.4, containing phosphatase inhibitors (10 mM NaF, 1 mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and the protein concentration was adjusted to 2 mg/ml using the Bradford method (Thermo Scientific ...
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bioRxiv - Genomics 2020Quote: ... Samples were pooled (150 ng of each reference samples and 100 ng of other samples) and the final library concentration was determined by qPCR (KAPA Library Quantification Kit, Roche). Sequencing was performed on a NextSeq 500 benchtop sequencer (Illumina ...
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bioRxiv - Cell Biology 2019Quote: 10 heads from animals at 8 DAE were resuspended in 100 μl of Hepes/NaOH 50mM with proteases and phosphatases inhibitors (Roche), samples were disrupted using a mechanical tip following two rounds of sonication of 10 sec each ...
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bioRxiv - Microbiology 2019Quote: ... The HA epitope tag was detected with either the anti-HA antibody (1:50 or 1:100; Anti HA high affinity 3F10, Cat. Nr. 11867423001, Roche), anti-V5 (1:50 or 1:100 ...
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bioRxiv - Microbiology 2019Quote: ... and lysed on ice using a lysis buffer (PBS, 0.1% SDS, 1% Triton X-100, EDTA-free complete protease inhibitor (Roche Diagnostics)) ...
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bioRxiv - Biochemistry 2019Quote: ... Cells were then washed twice with cold PBS and resuspended using a cell scraper into lysis buffer (50 mM Tris pH 8.0, 150 mM NaCl, 1% Triton X-100, 0.1 % NaDOC, PMSF and Roche Protease Cocktail). Cells were sonicated and lysates were centrifuged at 16,000xg for 15 minutes at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... resuspended in lysis buffer (1xPBS, 350 mM NaCl, 1% Triton X-100, 10% glycerol, EDTA-free Protease Inhibitor Cocktail (Roche), 30 mM imidazole) ...
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bioRxiv - Genetics 2019Quote: ... and RIPA double-detergent buffer (2% deoxycholate, 2% NP40, 2% Triton X-100 in RIPA homogenizing buffer) supplemented with a protease-inhibitor cocktail (Roche). Cells were subsequently incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Transfected HEK29T cells and COS-7 cells were washed and lysed with 1% Triton X-100 in PBS with protease inhibitor cocktail (Roche) at 4 °C for 1 hr ...
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bioRxiv - Biochemistry 2021Quote: ... the recombinant protein was expressed in HEK293T cells and released by 100 μM digitonin in PBS with protease inhibitor cocktail (Roche). The cell lysate prepared with the same method from HEK293T was used as the negative control.
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bioRxiv - Neuroscience 2021Quote: ... neurons were incubated live for 10 min at 37 °C with anti-HA rat monoclonal antibody (1:100, 11867423001, Roche) or anti-GFP mouse monoclonal antibody (1:200 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were lysed using RIPA buffer (50 mMTris–HCl, 10 mM MgCl2, 20% Glycerol and 1% Triton X-100) containing protease and phosphatase inhibitors (Roche). Protein concentration was determined by BCA assay (Pierce ...
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bioRxiv - Cancer Biology 2019Quote: ... and then resuspended in lysis buffer (10 mM Hepes-NaOH, 100 mM NaCl, 2 mM EDTA, 1 mM EGTA, 1 mM PMSF, 0.2% SDS, 0.1% sarkozyl, Roche proteases inhibitor). Sonication was performed with a Misonix sonicator (fifteen cycles of 20 seconds sonication interspaced by a pause of 50 seconds) ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were lysed in 300 µL FA lysis buffer (50 mM HEPES–KOH pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Triton X-100, 0.1% sodium deoxycholate, 1 mM PMSF, Roche protease inhibitor) with ∼1 mL ceramic beads using a Fastprep-24 (MP Biomedicals) ...
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bioRxiv - Neuroscience 2020Quote: ... frozen tissue was mechanically homogenized in TES buffer (4% Tris Base pH 7.5, 0.1 mM EDTA, 100 mM Na2Cl) containing protease inhibitor (PI) (cOmplete, EDTA-free Protease Inhibitor Cocktail (Roche, Mannheim, Germany)) with the VDI 12 homogenisator (VWR ...
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bioRxiv - Biochemistry 2020Quote: ... 50 mM sodium phosphate pH 8.0, 3% glycerol, 1% Triton X-100, 15 mM imidazole, and 1x protease inhibitor [Roche, Sigma]) and sonicated ...
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bioRxiv - Systems Biology 2020Quote: ... The filters were washed with 130 μl 50 mM ammonium bicarbonate and centrifuged again before overnight digestion with 100 μl 5 ng/μl trypsin (Roche) at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Tissue samples were homogenized in pre-chilled lysis buffer (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 1% Triton X-100, protease inhibitor cocktail (Roche 04693132001) and phosphatase inhibitor (Roche ...
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bioRxiv - Microbiology 2021Quote: ... The cell lysate was prepared by sonication of 2×108 cells on ice in 100 mM sodium phosphate buffer pH 7 including EDTA-free Complete Protease Inhibitors (Roche) and 0.01% TX-100 ...
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bioRxiv - Molecular Biology 2021Quote: ... in 48-well plates and transfected at 50-60% confluence with a total of 100 ng Dual-pMIR-report plasmids using X-tremeGENE 9 DNA Transfection Reagent (Roche), followed by transfection with mimics (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... washed in cold PBS and resuspended in 100 – 300 μl DI-water with 1mM PMSF and cOmplete EDTA-free protease inhibitor tablet (Roche). Resuspended pellets were freeze-thawed three times with liquid nitrogen/37°C water bath ...
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bioRxiv - Molecular Biology 2021Quote: ... Then, spikelets were dehydrated through a series of ethanol (50%, 70%, 85%, 95%, and 100%) and embedded in paraffin (Roche). Cross-sections of 10 µm thickness were cut using the rotary microtome followed by a dehydration gradient of 95% ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... rRNA-depleted RNA-seq libraries were prepared from 100-1,000ng RNA per sample using the KAPA RNA HyperPrep Kit with RiboErase (HMR) (Roche, #KK8560). RNA fragmentation was performed at 94°C for 6 minutes and 10 PCR cycles were used during library amplification with TruSeq single-index adapters (Illumina ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated overnight at 4 °C in digoxigenin antibodies conjugated to horseradish peroxidase (anti-digoxigenin-POD; Fab fragment; 1:100; Roche), rinsed in TBS (0.1 M Tris-HCl with 0.9% NaCl ...
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Programmed ER fragmentation drives selective ER inheritance and degradation in budding yeast meiosisbioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended by bead beating for 5 min in 100 μL TE supplemented with 3 mM and 1x protease inhibitors (Roche) with 100 μL acid-washed glass beads ...
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bioRxiv - Cell Biology 2020Quote: POT-2 expression pellets were resuspended in Tris buffer (50 mM Tris/HCL pH 7.5, 100 mM NaCl, 10 mM MgCl2, 1x EDTA-free protease inhibitor (Roche, #4693132001)) and divided into 2 mL flat lid micro tubes containing 0.1 mm zirconia beads (Carl Roth ...
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bioRxiv - Cancer Biology 2021Quote: ... and cells were lysed in 300 μl Triton X-100 lysis buffer supplemented with EDTA-free protease inhibitor mix (Roche). 100 μl supernatant and 100 μl diluted cell lysates were independently incubated with 100 μl AP substrate p-nitrophenyl phosphate (PNPP ...
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bioRxiv - Immunology 2022Quote: ... Precipitated pellet was then resuspended in 100 μL of RIPA buffer plus laemli supplemented with 5 mM diisopropylfluorophosphate (DFP) and protease inhibitor cocktail (Roche) and heat denaturated for 10 min at 95°C ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (1%vol/vol)) containing PMSF (final concentration 1 mM) and complete protease inhibitor cocktail (without EDTA, from Roche). A 500 μl volume of glass beads was added to the tubes ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was removed followed by the pellet being triturated in 100 μL of lysis buffer (7M Urea, 2M Thiourea, 20mM Tris-HCl) and 1x protease inhibitor (Roche cOmplete mini-inhibitor ...
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bioRxiv - Developmental Biology 2022Quote: ... Ten micrometer cryo-sections were permeabilized with 0.5% Triton X-100/PBS for 10 min and then incubated with blocking buffer (1% blocking reagent [Roche]/TNT) for 1 h ...
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bioRxiv - Cell Biology 2022Quote: ... washed with PBS and resuspended in lysis buffer (0.5% Triton X-100, 4% w/v SDS, 0.5xPBS) supplemented with protease inhibitor cocktail (Roche, Basel, Switzerland). The lysates were then resuspended in 5 x SDS-PAGE loading buffer containing 25mM DTT ...
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bioRxiv - Immunology 2022Quote: One million cells were collected by centrifugation and resuspended in cold lysis buffer (1% Triton X-100 in PBS) containing protease inhibitors (Complete protease inhibitor cocktail, Roche) and was incubated on ice for 30 minutes ...
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bioRxiv - Cell Biology 2022Quote: Cells were harvested in cold PBS and subsequently lysed in 1% (v/v) Triton X-100 in PBS supplemented with Protease Inhibitor Cocktail (Roche), N-ethylmaleimide and sodium orthovanadate ...
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bioRxiv - Neuroscience 2020Quote: ... Protein aggregation was evaluated by western blot in total cell extracts prepared in 1% Triton X-100 in PBS containing proteases and phosphatases inhibitors (Roche). Protein quantification was performed with the Pierce BCA Protein Assay Kit (Thermo Scientific).
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bioRxiv - Neuroscience 2020Quote: Surface expression of HA-C99 and cc-del(X) fusion proteins was measured with monoclonal HA-antibody (Roche 1:100) by flow cytometry as described previously (Staerk et al. ...
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bioRxiv - Immunology 2020Quote: 100 ng TCRB chain cDNA was used as template for PCR with KAPA2G Fast Multiplex Mix (Kapa Biosystems, Wilmington, MA). Forward TCRB FR3 primers from the Full ...
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bioRxiv - Plant Biology 2021Quote: ... For IPs 0.3 g of flower buds were ground in 1.5 ml of ice-cold lysis buffer (50 mM Tris-HCl pH 8, 50 mM NaCl, 1 % Triton X-100, supplemented with Roche cOmplete ...
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bioRxiv - Molecular Biology 2020Quote: ... resuspended in nuclear extraction buffer (20mM HEPES pH7.5, 10% glycerol, 350mM NaCl, 0.1% TritonX-100, 1mM DTT, 1mM MgCl2, 0.5mM EDTA, 10mM NaF, Roche protease inhibitor cocktail) and incubated for 30 min at 4°C on a rotator ...
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bioRxiv - Molecular Biology 2020Quote: ... The crude nuclear pellet was rinsed in wash solution (20mM HEPES pH7.5, 10% glycerol, 150mM NaCl, 0.1% TritonX-100, 1mM DTT, 1mM MgCl2, 0.5mM EDTA, 10mM NaF, Roche protease inhibitor cocktail) twice ...
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bioRxiv - Cell Biology 2020Quote: ... TNbuffer 10 min at RT and TNT buffer 15’ at RT) and by the incubation of cryo-section using anti-digoxigenin-ap fab fragments (1/100, #11093274910, Roche) dissolved in TNbuffer for 2 hours at RT ...
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bioRxiv - Microbiology 2021Quote: ... and 100 ng of DNA was converted into paired-end Illumina sequencing libraries using KAPA HyperPlus library preparation kit (Roche) with the KAPA unique dual-indexed adapters (Roche) ...
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bioRxiv - Genetics 2022Quote: ... APRE-19 were cultured in a 100 mm dish for 24 hours and then lysed with 1×NP40 lysis buffer supplemented with protease inhibitors Coaktails (Roche) and phosphatase inhibitors(Roche) ...
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bioRxiv - Genomics 2022Quote: ... The eluted material and input were incubated for 6 h at 65°C to reverse crosslinks and were treated with 100 ng RNaseA (Roche) for 1 h at 50°C ...
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bioRxiv - Cell Biology 2019Quote: ... 0.1% SDS,1% NP-40 and 1% Triton X-100) supplemented with 1 mM PMSF and protease inhibitor cocktail (Roche) at 4°C for 20 min ...