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Citations for Roche :
2951 - 3000 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The blocking solution was removed and replaced with antibody solution containing 1% heat inactivated goat serum and a 1:5000 dilution of anti-DIG-AP antibody (Roche). Slides were then incubated overnight at 4°C in a humidified chamber ...
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bioRxiv - Microbiology 2023Quote: ... Samples were centrifuged for 10 min at 16000 xg and resuspended in 300 µl PBS containing protease inhibitors (cOmplete, Roche). A 16 µl sample was added to 5 µl of 5x SDS sample buffer (300mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: Spleens were injected with HBSS (with Ca2+ and Mg2+) containing 1 mg/mL of collagenase D and 10 μg/mL of DNase I (Roche) and incubated for 20 minutes at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1mM DTT [modified from Cilia Wash Buffer in (Gaertig et al., 2013)] containing the following inhibitors: 1x cOmplete mini EDTA-free protease inhibitors (Roche), 1x PhosSTOP EASY phosphatase inhibitor (Roche ...
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bioRxiv - Immunology 2023Quote: ... and digested using a homogenizer in 500 ml PBS containing 100 mg/ml collagenase/dispase (10-269-638; La Roche). Samples (100 ml each ...
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bioRxiv - Cell Biology 2023Quote: ... 0.1% sodium dodecyl sulfate) containing protease and phosphatase inhibitors (Pierce Protease Inhibitor Mini Tablet, EDTA-free, Thermo Scientific; PhosSTOP, Roche) for 45 minutes at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Infected cells were selected 48 h after transduction with antibiotic supplemented media containing either 0.1 mg/mL Hygromycin B (Roche, H3274), 5 μg/mL Blasticidin S Hydrochloride (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... the cell monolayers were scraped into 10 ml KPBS (136 mM KCl and 10 mM KH2PO4, pH 7.25, containing protease (Roche, 1836153001) and phosphatase inhibitors (PhosSTOP Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Washes were followed by blocking in 10% heat-inactivated sheep serum for 1-3 hours and incubation in buffer containing sheep antidigoxigenin antibody (Roche) at 1:5000 dilution for 16-20 hours at 4oC ...
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bioRxiv - Neuroscience 2023Quote: ... slides were incubated at 37oC in a staining solution containing nitro blue tetrazolium and 5-bromo-4-chloro-3-indolyl-phosphate (Roche) for 20-24 hours ...
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bioRxiv - Physiology 2023Quote: ... in PEB Buffer (150 mM NaCl, 50 mM Tris-HCl, 5mM EDTA, 1% NP-40) containing proteases (cOmplete™, Roche) and phosphatase (Phosphatase Inhibitor Cocktail 2 ...
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bioRxiv - Neuroscience 2023Quote: ... pons and frontal cortex tissues were obtained by homogenization in cold phosphate-buffered saline (PBS) containing protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Neuroscience 2023Quote: ... were homogenized in lysis buffer (PBS containing 0.3% Triton X-100, 1 mM sodium orthovanadate and protease inhibitors (Roche, 4693132001). Total protein of each sample was determined using the Bio-Rad DC kit (Bio-Rad ...
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bioRxiv - Neuroscience 2023Quote: Frozen brain tissues from human and animals were used to prepare 10% (w/v) homogenates in RIPA buffer containing PI and PhosStop (Roche). Briefly ...
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Bni5 tethers myosin-II to septins to enhance retrograde actin flow and the robustness of cytokinesisbioRxiv - Cell Biology 2023Quote: ... Cells were then lysed by sonication 6 times for 15 sec each in one of the following buffers each containing the Protease Inhibitors Cocktail tablets (Roche) and 1 mg/mL lysozyme (L6876 ...
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bioRxiv - Neuroscience 2023Quote: ... Conditioned media was collected into cooled tubes on ice (already containing 20× pre-dissolved protease inhibitor cocktail (PI; cOmplete EDTA-free, Roche) and subsequently centrifuged at 500×g and 5,000×g for 5 min at 4°C ...
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bioRxiv - Physiology 2023Quote: ... The signal was then visualized by incubating the slides for 48 hours in a solution containing 0.33 mg/mL NBT (Nitro-blue tetrazolium chloride, Roche, 11383213001) and 0.165 mg/mL BCIP (5-Bromo-4-chloro-3-indolyl phosphate ...
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bioRxiv - Cell Biology 2023Quote: ... and the cell pellet was resuspended in 1 ml vPBS (PBS, 46 mM sucrose, 10 mM glucose) containing EDTA-free protease inhibitors (Roche). The washed cells were pelleted by centrifugation (750xg ...
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bioRxiv - Genomics 2023Quote: One hundred mg of 14-day-old seedlings were ground and nuclei were isolated with 4°C Buffer (0,25M Sucrose, 10mM Tris-HCl, 10mM MgCL2, 1% Triton, 5mM ß- mercaptoethanol) containing proteinase inhibitor cocktail (Roche) and filtered in 63 µm ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysates were prepared using a 1% triton X-100 containing lysis buffer (CST) supplemented with protease inhibitor cocktail (Roche) on ice for 15 min ...
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bioRxiv - Systems Biology 2024Quote: ... The lungs were perfused through the pulmonary artery and inflated via the airway with DMEM containing 1 mg/ml Collagenase/Dispase (Roche), 3 U/ml Elastase (Worthington) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were extracted by resuspending cells from 6-well plates in 100 μl of RIPA or 7M Urea Buffer containing protease inhibitor (11873580001, Roche) and ...
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bioRxiv - Synthetic Biology 2024Quote: ... These barcode primers were combined separately with another constant primer to create short double-stranded fragments containing the barcode flanked by BsaI cut sites in a single cycle of PCR using Kapa HiFi (Roche). 1μL of this reaction was added into a second Kapa HiFi (Roche ...
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bioRxiv - Immunology 2023Quote: Cells were washed with PBS and lysed with ice-cold CelLytic M Cell Lysis Reagent containing protease inhibitor cocktail (Roche) and phosphatase inhibitors (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: Protein extracts from DCN and frontal cortex tissues were obtained by homogenization of brain tissue in cold phosphate-buffered saline (PBS) containing protease inhibitor cocktail (cOmplete Protease Inhibitor Cocktail, Roche) and phosphatase inhibitors (PhosSTOP ...
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bioRxiv - Immunology 2023Quote: ... and lysed in RIPA buffer (CST; cat# 9806S) or NP-40 lysis buffer containing PhosSTOP phosphatase inhibitor (Roche; cat# 04906845001) and EDTA-free protease inhibitor (BioShop ...
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bioRxiv - Bioengineering 2024Quote: ... livers were perfused at low flow for about 10 min with digestion buffer (low-glucose DMEM supplemented with 1 mM Hepes) containing freshly added Liberase (32 μg/ml; Roche). Digestion was stopped using isolation buffer (low-glucose DMEM supplemented with 10% FBS) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were re-suspended in 500 µL of lysis buffer (PBS containing 1 % Triton-X 100 and complete protease inhibitors [Roche]). DAPI was added to lysis buffer (final concentration 5 µM ...
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bioRxiv - Neuroscience 2023Quote: ... containing 10mL 1X artificial CSF (pH 7.4) supplemented with 1x cOmplete mini EDTA-free protease inhibitor cocktail tablet (Roche, 11836170001) per 10mL ...
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bioRxiv - Pathology 2024Quote: ... The proteins were redissolved in 8 M Urea/50 mM TEAB buffer containing protease- and phosphatase inhibitors (EDTA free cOmpleteTM, PhosSTOPTM Roche) and treated 30 minutes at 37 °C with 50 units/100 µg tissue benzonase (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were lysed 80 h post seeding in RIPA buffer (Thermo, 89901) containing cOmplete EDTA-free protease inhibitor cocktail (Roche). Lysate were centrifuged at 15000 rpm for 15 min ...
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bioRxiv - Neuroscience 2023Quote: ... were washed with ice cold PBS and incubated in 100 µL of RIPA buffer containing protease inhibitors (Complete ULTRA tablets, Roche). Cells were gently agitated on orbital shaker for five minutes before being spun down at 18 000g for 15 minutes ...
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bioRxiv - Cell Biology 2024Quote: ... dissected TA muscles were first digested with 10ml muscle digestion buffer (DMEM containing 1% penicillin/streptomycin, 0.125mg/ml Dispase II (Roche, 04942078001), and 10mg/ml Collagenase D (Roche ...
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bioRxiv - Immunology 2024Quote: ... and lung lobes were finely minced and incubated in 15 mL of complete media containing 1 mg/mL collagenase (Roche) and 17 U/mL DNase I (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... desoxycholic acid-Na-salt 1%, SDS 0.1%) (Serva electrophoresis, Germany) containing protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Switzerland). Western blots were performed following routine protocols67 ...
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bioRxiv - Neuroscience 2024Quote: ... The mixture for qPCR reaction was prepared in a final volume of 20 μl containing 1 μl cDNAs and 10 μl of LightCycler 480 SYBR Green I Master (Roche) in the presence of primers at 500 nM ...
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bioRxiv - Genetics 2024Quote: Total protein was isolated from ∼15 larval brains or adult fly heads by homogenization of fly tissue in Radioimmunoprecipitation Assay (RIPA) buffer containing protease inhibitors (Roche). Approximately 20-30µg of total protein was separated using 4-20% SDS-PAGE (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... Total cell lysates were extracted from CAR T cells using radioimmunoprecipitation assay (RIPA) buffer (Thermo) containing protease inhibitor cocktail (Roche). Total protein concentration was determined using Pierce BCA assay (Thermo) ...
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bioRxiv - Immunology 2024Quote: Human and murine cells were incubated in lysis buffer (20 mM Tris-HCl, 150 mM NaCl, 1 % NP-40, 5 % glycerol) containing 1X protease inhibitor cocktail (Roche) on ice ...
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Direct and indirect regulation of β-glucocerebrosidase by the transcription factors USF2 and ONECUT2bioRxiv - Molecular Biology 2024Quote: ... cells were washed in PBS and lysed in 50 mL McIlvaine buffer containing EDTA-free cOmplete Mini Protease Inhibitors (Roche). Cells were shaken at 4°C and 450 rpm for 10 min followed by one hour of incubation at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Genomic DNA containing either the on-target or off-target sites was amplified with KAPA HiFi HotStart DNA polymerase (Roche) or SUN-PCR Blent (SUN GENETICS ...
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bioRxiv - Neuroscience 2024Quote: ... life-Tein LLC) was mixed with 50µl of PBS containing 1% Tween20 and 5ng/mL anti-HA-Peroxidase 3F10 antibody (Roche #12013819001) and transferred to the wells ...
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bioRxiv - Physiology 2024Quote: ... the legs of each genotype were homogenized in Radioimmunoprecipitation assay buffer containing a complete Protease Inhibitor Cocktail (Roche, Basel, Switzerland). A Filter Aided Sample Preparation (FASP ...
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bioRxiv - Genetics 2023Quote: ... Final libraries were quantified by qPCR using the KAPA Library Quantification Kit (Complete kit; Universal) (Roche) and sequenced on a MiSeq sequencer using a 300-cycle v2 kit (Illumina).
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bioRxiv - Evolutionary Biology 2024Quote: ... Digoxigenin (DIG)-labelled RNA probes were then synthesized with the kit DIG RNA Labelling Kit (Roche) with T7 RNA polymerase and DIG–dUTP ...
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bioRxiv - Synthetic Biology 2021Quote: ... KAPA2G Robust PCR kits (Roche KK5005) were used to perform colony PCR and multiplex PCR for a screening of Yeast Artificial Chromosomes (YACs) ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification analysis was performed by Illumina Library Quantification Kit (KAPA Biosystems, KK4824). The library was sequenced on an Illumina NovaSeq system or Nextseq 500 instrument.
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bioRxiv - Genetics 2021Quote: ... with KAPA HiFi Uracil+ Kit (Roche) used in place of Enhanced PCR Mix for the amplification step ...
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bioRxiv - Molecular Biology 2020Quote: ... and KAPA library quantification kit (Roche). RNA-seq libraries were sequenced using the Illumina NextSeq 500/550 platform with the High Output v2 kit (150 cycles ...
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bioRxiv - Molecular Biology 2021Quote: ... KAPA-SYBR FAST qPCR kit (Roche) was used to quantify the libraries ...