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Citations for Roche :
3151 - 3200 of 9048 citations for Cow WAP kazal immunoglobulin kunitz and NTR domain containing protein 2 WFIKKN2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... After that pelleted beads were gently resuspended in washing buffer containing 4xTBS, 5% NP-40, phosphatase inhibitors (10mM NaF, 1mM Na3VO4) and protease inhibitors (cOmplete®, Roche) and washed 10 times with gentle agitation ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Molecular Biology 2021Quote: ... were homogenized on ice with a hand-held OMNI TH homogenizer (OMNI International) with ice-cold RIPA lysis buffer containing Complete Mini Protease Inhibitor Cocktail and PhosSTOP Phosphatase Inhibitor Cocktail (Roche Diagnostics). In cell culture experiments ...
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bioRxiv - Immunology 2021Quote: ... was added to the PBMCs for 5 minutes before transferring drop-wise to a 10ml volume of warmed media (RPMI/20%FBS) containing 200U/ml of DNase I (Roche, Switzerland). After 10 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... HPV DNA capture was performed with NimbleGen’s SeqCap EZ Custom Library containing probes for all HR HPV types (Roche NimbleGen, Inc.,) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2019Quote: ... Cellular lysates were generated by washing transfected cells with PBS and lysing cells in NP40 buffer containing cOmplete™ protease inhibitor cocktail (Roche). To detect presence of VP40 ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplification was carried out in 12 μL volumes containing 6 μL of High Resolution Melting Master (Roche Applied Science, Germany), 0.24 μL of each 10 μM primer ...
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bioRxiv - Cell Biology 2020Quote: ... For the neuraminidase treatment each well was supplied with 500 μL of a solution containing 50 mU/mL neuraminidase (Roche Diagnostics) in cold PBS+++ pH adjusted to 5.5 ...
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bioRxiv - Genetics 2019Quote: ... was used as a hypotonic solution and cells were dropped onto a slide with 1% PFA containing protease inhibitors (Roche 1836153). After 3h at 4°C slides were washed and blocked with 0.5x blocking buffer (1.5% BSA ...
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bioRxiv - Microbiology 2019Quote: ... The spores were mixed in phosphate buffered saline (PBS) containing protease inhibitor cocktail (cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail, Roche) and lysed using a Precellys 24 homogenizer (Bertin Technologies ...
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bioRxiv - Immunology 2021Quote: ... the lamina propria was dissected into small pieces with scissors and incubated in digestion buffer (RPMI 1640 containing 0.17 mg/mL Liberase TL (Roche, Basel, Switzerland) and 30 µg/mL DNase (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hours at room temperature and left to incubate for 24 hours with blocking buffer containing a 1:200 dilution of anti-DIG (Roche, 11207733910) or anti-fluorescein antibody conjugated with POD (Roche ...
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bioRxiv - Cancer Biology 2020Quote: ... washed twice in PBS and resuspended in 100 μL annexin V incubation buffer (10 mM HEPES/NaOH, pH 7.4, 140 mM NaCl, 5 mM CaCl2) containing 1% annexin V FLOUS (Roche Molecular Biochemicals) and 500 μg/μl PI stain ...
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bioRxiv - Cancer Biology 2020Quote: Cell lines were washed with ice-cold PBS and scraped into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested from frozen plates by scraping into RIPA buffer containing cOmplete Mini EDTA-free Protease Inhibitor Cocktail (Roche 11836170001) and PhosSTOP Phosphatase Inhibitor Cocktail Tablets (Roche 04906845001 ...
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bioRxiv - Cell Biology 2022Quote: 3T3-L1 adipocytes were washed with ice-cold PBS and harvested in ice-cold HES-I buffer (20 mM HEPES, pH 7.4, 1 mM EDTA, 250 mM sucrose containing protease inhibitors mixture (Roche Applied Science)) ...
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bioRxiv - Cell Biology 2022Quote: Murine cells or aortic tissue were lysed in RIPA buffer containing complete protease inhibitor and phosphoSTOP phosphatase cocktails (Roche Applied Science). Lysate was centrifuged at 10,000 g for 10 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were scraped on ice in 500 µL of ice-cold PBS containing cOmplete™ Protease Inhibitor Cocktail EDTA-free (Roche) and Phosphatase Inhibitor Cocktails 1 & 2 (Sigma) ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were scraped on ice in 500 µL of ice-cold PBS containing cOmplete™ Protease Inhibitor Cocktail EDTA-free (Roche) and PhosStop (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... brains homogenized in 1xPBS containing 1% SDS and protease inhibitors (Complete EDTA-free Protease Inhibitor cocktail, Roche, Sigma Aldrich, Cat#11873580001) and boiled for five min ...
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bioRxiv - Microbiology 2022Quote: ... South Korea) was made in 10 ml per brain of digestion cocktail containing 0.5 mg/ml DNase I (Roche, Basel, Switzerland) and 1 mg/ml Collagenase A (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... AML blast cells were incubated in 1 ml of ice-cold 0.1 M Na2CO3 containing complete protease inhibitor (Roche, Penzberg, Germany) and phosphatase inhibitor PhosSTOP (Roche ...
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bioRxiv - Immunology 2021Quote: ... Pancreas and liver tissue extract were prepared in RIPA buffer containing a mixture of protease inhibitors (Complete protease inhibitor, Roche Diagnostics).
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bioRxiv - Immunology 2021Quote: ... We resuspended the glomerular fraction in 1mL HBSS buffer with Ca2+ and Mg2+ (Hyclone, Logan, UT) containing 0.1-0.2 mg/mL Liberase™Research Grade (Roche, Mannheim, Germany), and incubated glomeruli in 24-well tissue culture plates at 37°C for 24 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Brains were homogenised in HB buffer (20 mM NaP pH 7.4, 30 mM NaCl containing phosphatase inhibitors (PierceTM) and protease inhibitors (Complete, Roche Diagnostics GmbH)) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... followed by perfusion with Hanks’ balanced salt solution with Mg2+ and Ca2+ containing 0.1 mg/mL of Liberase (Roche Applied Science). Following the removal of the liver ...
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bioRxiv - Neuroscience 2020Quote: ... Brain samples were thawed at room temperature and ground in ice-cold Tris-buffered saline (TBS) containing a Protease Inhibitor Cocktail Tablet (Roche, Switzerland), with a final dilution of 1:100 ...
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bioRxiv - Developmental Biology 2020Quote: ... phosphate-buffered saline containing 1X complete protease inhibitors (concentration recommended by use of soluble EDTA-free tablets sold by Roche #11873580001) and 10 mM sodium butyrate as a histone deacetylase inhibitor (Sigma # B5887) ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.5% N-Lauroylsarcosine) and resuspended in 100 μl complete LB3 (LB3 containing 1x Roche cOmplete Mini, EDTA-free Protease Inhibitor Cocktail (Roche, #04693159001)) ...
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bioRxiv - Developmental Biology 2020Quote: ... and washed in PBS-PVA to remove proteins from the medium. All samples were lysed in RIPA buffer (cat. #P0013C, Beyotime, CHN) containing protease inhibitor cocktail (cat. #11697498001, Roche, USA). The samples were loaded into 5×SDS loading buffer (cat ...
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bioRxiv - Plant Biology 2020Quote: ... in a total volume of 6 μL containing 0.5 mM of each specific primer and 3 μl of SYBR Green I Master Mix (Roche Applied Science). The second derivative maximum method was used to determine Cp values and PCR efficiencies were determined using LinRegPCR software (http://LinRegPCR.nl) ...
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bioRxiv - Synthetic Biology 2019Quote: ... The cell pellet was resuspended in 45 mL of start buffer (20 mM Tris, pH 8.0, 500 mM NaCl, 10% glycerol, containing a protease inhibitor cocktail from Roche Applied Science) and lysed by homogenization at variable pressures of 10,000–15,000 psig and centrifuged at 25,000 × g for 1 h at 4°C ...
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bioRxiv - Physiology 2021Quote: ... Collagen was then extracted from the tissue samples using a salt extraction buffer (1 M NaCl, 25 mM EDTA, 50 mM Tris-HCl, pH 7.4) containing protease inhibitors (Roche, Basel, Switzerland). Samples were extracted overnight at 4°C with agitation ...
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bioRxiv - Microbiology 2020Quote: ... cells were harvested by centrifugation and resuspended in 20 mL of lysis buffer (20 mM Tris-HCl, 500 mM NaCl [pH8], containing a protease inhibitor cocktail (Complete®, Roche)) ...
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bioRxiv - Immunology 2020Quote: ... Real-time PCR was performed in a final reaction volume of 20 µL containing 10 µL FastStart universal probe master (Roche Diagnostics), 50 ng/µL lambda DNA (New England Biolabs) ...
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bioRxiv - Immunology 2021Quote: ... and were enzymatically digested in a shaker incubator at 37°C for 40 minutes in RPMI medium containing 1 mg/ml Collagenase D (Roche-Diagnostics), 1 mg/ml hyaluronidase and 50 U/ml DNase I (Sigma Aldrich) ...
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bioRxiv - Microbiology 2019Quote: ... For cell disruption the pellets were thawed on ice and resuspended in buffer A (50 mM Tris-HCl, 1mM EDTA, pH 8.0) containing cOmplete Protease Inhibitor (Roche, Basel, Switzerland). This cell suspension was then treated with a French pressure cell for three passages at 172MPa ...
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bioRxiv - Physiology 2021Quote: ... containing cOmplete EDTA-free protease inhibitor cocktail at a concentration of 1 tablet/7 ml (Roche Applied Science, Indianapolis, IN, USA)) by repeated passage through a 20-gauge needle to obtain plasma membrane-enriched preparations ...
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bioRxiv - Microbiology 2021Quote: Full-length wild-type human APN encoding plasmid was used as a template to create a glycosilation sequence at position N739 (NWR to NWT change) in a reaction mixture containing: 2X Kapa HiFi (Kapa biosystems) and the following primers ...
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bioRxiv - Neuroscience 2020Quote: ... Beads containing cDNA were then eluted and cDNA was amplified for 21 cycles using Kapa HiFi Hot start ready mix (Roche #07958935001). Amplified cDNA was then purified (GC biotech CPCR-0050 ...
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bioRxiv - Molecular Biology 2021Quote: ... Library Enrichment was performed in a 30 μl reaction volume containing 20 μl DNA from the previous step and the following final concentrations: 1X KAPA buffer (Roche #KK2502), 0.3 mM each dNTP (Roche #KK1017) ...
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bioRxiv - Cancer Biology 2021Quote: 1 μl sample from Denaturation was transferred to the qPCR plate well containing the qPCR Mix (Nuclease-free water + KAPA SYBR FAST qPCR Master Mix (KAPA Biosystems) + cDNA Primers (from Visium Reagent kit)) ...
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bioRxiv - Microbiology 2020Quote: Frozen fecal samples were diluted in lysis buffer [100mg feces per 1 ml of PBS containing protease inhibitors (Roche, Indianapolis, IN)] ...
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bioRxiv - Molecular Biology 2021Quote: ... washed with ice-cold PBS (w/o calcium and magnesium) and lysed immediately in RIPA-Buffer containing phosphatase inhibitor cocktail (PhosStop, Roche, USA) and protease inhibitor cocktail (HaltProtease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2022Quote: ... amplification was performed in a 20-μl reaction mixture containing 1× Light Cycler Fast Start DNA master hybridization probes (Roche Diagnostics), 4 mM MgCl2 ...
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bioRxiv - Developmental Biology 2022Quote: ... at 15-20k cells/ well in Cardiac Mesoderm Patterning Media One made up of CDM containing ROCKi and for all protocols besides the LV cardioids 1 µg/ml of insulin (Roche, #11376497001) plus specific factors depending on cardioid subtype (see below) ...
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bioRxiv - Cancer Biology 2022Quote: Cancer transplantation was performed as described in the section “spatial transcriptomics.” The obtained livers were minced and dissociated in RPMI1640 containing DNase I (100 μg/ml: Roche, Switzerland) and collagenase IV (1 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... the frozen human tumors and normal tissues and the tumors from animal xenograft studies were lysed in RIPA lysis buffer containing protease and phosphatase inhibitors (Roche Inc), and sonicated for 20s ...
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bioRxiv - Immunology 2022Quote: ... these tissues were dissected and incubated for 1h at 37ºC in the enzymatic cocktail containing Colleagenase D (Roche, 1 mg/ml) and DNAse I (Roche ...
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bioRxiv - Immunology 2022Quote: The dorsal halves of the ear skin of mice were collected and incubated for 70 min at 37°C in cRPMI containing 0.33 mg/ml Liberase TL (Roche, Basel, Switzerland) and 0.05% DNase I (Sigma-Aldrich ...