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Citations for Roche :
2201 - 2250 of 8008 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2020Quote: ... RT-qPCRs were performed using 1× LightCycler 480 SYBR Green I Master (Roche), 0.5 μM primers (Suppl ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-qPCR was performed on a LightCycler® 480 II (Roche Life Science) using SYBR® Premix Ex Taq(tm ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was subsequently performed as described [23] using Sybr green (Roche, 06924204001) on The LightCycler 480 System ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using LightCycler 480 SYBR Green I master mix (Roche). For quantification of gene expression ...
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bioRxiv - Immunology 2022Quote: ... slides were blocked at RT for 1 h in 1x Blocking reagent (Roche) and incubated at 4°C over night with a phospho-histone H2AX (pSer139 ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR assays were carried out on LightCycler LC480 II (Roche Diagnostics, Germany) using SYBR Green Jumpstart Taq Ready mix (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR master mix (Kapa Biosystems) with gene-specific primers (Supplementary Table 6 ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed in a LightCycler® 2.0 instrument (Roche, Mannheim, Germany), using 45 cycles of the following program ...
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bioRxiv - Genetics 2019Quote: ... RT-qPCR reactions were carried out in quadruple on a LC480 machine (Roche). The reaction mix consisted of cDNA ...
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bioRxiv - Microbiology 2024Quote: ... RT-qPCR was then performed using SYBR green master mix (Roche, Indianapolis, IN) and a Light Cycler 480 Instrument II (Roche ...
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bioRxiv - Microbiology 2024Quote: ... and RT-qPCR was performed using SYBR green master mix (Roche, Indianapolis, IN) and a Light Cycler 480 Instrument II (Roche ...
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bioRxiv - Cancer Biology 2024Quote: ... The RT-qPCR cycling analysis was performed using a LC-480 device (Roche). qBasePlus software 3.2 (www.biogazelle.com ...
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bioRxiv - Molecular Biology 2022Quote: ... a minimum of 50ng of RNA was used to prepare cDNA (Roche RT) and splice isoform specific PCRs were performed (Sigma Aldrich High fidelity Taq ...
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bioRxiv - Immunology 2023Quote: ... The RT-qPCR was run on a Roche LightCycler 480 (Roche Diagnostics Ltd) using the conditions outlined in Table 4.
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bioRxiv - Biophysics 2022Quote: GGCAGCGCTACCATAACGGA-3’) (11) by RT-qPCR was performed using LightCycler 480 II (Roche). GAPDH mRNAs were used as a loading control (14) ...
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bioRxiv - Plant Biology 2022Quote: ... Expression analysis by RT-qPCR was performed using SYBR Green master I (Roche) and the LightCycler® 96 system following a standard protocol (40 cycles ...
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bioRxiv - Cell Biology 2023Quote: ... RT-qPCR was performed with the LightCycler® 480 Instrument (Roche Life Science) and data were analyzed according to manufacturer’s instruction (Roche LightCycler® 480 software ...
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bioRxiv - Molecular Biology 2023Quote: ... The RT-qPCR was run on a LightCycler 480 II (Roche Applied Science) using 384 well plates ...
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bioRxiv - Plant Biology 2023Quote: ... RT-qPCR was performed using LightCycler 480 SYBR Green I Master mix (Roche) and run in a LightCycler 480 System (Roche) ...
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bioRxiv - Biochemistry 2023Quote: ... The expression of selected genes was measured by RT-qPCR (LightCycler 480; Roche), cDNA was prepared from 1 µg of RNA using QuantiNova Reverse Transcription Kit (Qiagen) ...
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bioRxiv - Developmental Biology 2023Quote: ... RT qPCR samples were prepared using the KAPPA SYBR R fast (Roche, KK4611). Primer Sequences:
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bioRxiv - Cell Biology 2024Quote: ... RT-qPCR was performed with a Roche Lightcycler 480 (Roche Molecular Systems, Inc.). Each reaction contained 1µl of cDNA ...
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bioRxiv - Genetics 2024Quote: ... RT- qPCR was performed in triplicate with the LightCycler 480 instrument (Roche Diagnostics) and LightCycler 384-well plates with sealing foil (Roche Diagnostics) ...
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bioRxiv - Cell Biology 2020Quote: ... Target regions were amplified by using specific PCR primers (ETV2_F: CACTCGGGATCCGTTACTCC; ETV2_R: GTTCGGAGCAAACGGTGAGA, KDR_F: CAAGCCCTTTGTTGTACTCAATTCT; KDR_R: ATTAATTTTTCAGGGGACAGAGGGA) and KAPA HiFi HotStart PCR kit (KAPA Biosystems, Cat No. KK2601). Sanger sequencing (Genewiz ...
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bioRxiv - Microbiology 2020Quote: ... Both the pET28a vector and the Rv1630 gene amplification product (1446 bp) were purified using the PCR quick spin kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Pathology 2022Quote: ... We used genomic DNA isolated from ear tissue with a High Pure PCR Template Preparation Kit (Roche Diagnostics, Basel, Switzerland). The transgene was detected using RT2 qPCR Primer Assays from Qiagen (Venlo ...
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bioRxiv - Genomics 2022Quote: ... The libraries were quality controlled on an Agilent 2100 Bioanalyzer with the DNA 7500 assay for size and the concentration was estimated using quantitative PCR with the KAPA Library Quantification Kit Illumina Platforms (Roche).
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bioRxiv - Genomics 2020Quote: ... Entire material of one conversion was equally distributed to a 96-well plate and amplified via PCR using KAPA HiFi Uracil+ Kit (Roche) in a total volume of 16µl ...
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PHF2 regulates homology-directed DNA repair by controlling the resection of DNA double strand breaksbioRxiv - Molecular Biology 2019Quote: ... cDNA synthesis and PCR amplification were carried out in the same tube using the qScript One-Step SYBR Green qRT-PCR Kit (Quantabio) and a LightCycler480 II (Roche). All reactions were performed in triplicate ...
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bioRxiv - Microbiology 2019Quote: ... RNA transcripts of the cloned 5’UTRB7 fragments were produced in vitro from the resulting purified PCR products by using the T7 Transcription kit (Roche) and labeled by using the RNA 3’ end biotinylation kit (Pierce) ...
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bioRxiv - Plant Biology 2019Quote: ... with 340bp insert size according to KAPA Library Preparation Kit with no PCR Library Amplification/Illumina series (Roche-Kapa Biosystems) protocol and sequenced on HiSeq2000 (v4 ...
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bioRxiv - Plant Biology 2019Quote: ... with 340bp insert size according to KAPA Library Preparation Kit with no PCR Library Amplification/Illumina series (Roche-Kapa Biosystems) protocol and sequenced on HiSeq2000 (v4 ...
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bioRxiv - Genomics 2021Quote: ... were used to prepare single-end (SE) libraries with an insert size of 300 bp using a PCR-free workflow with the KAPA HyperPrep Kit (Roche) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... were generated cloning each enhancer from the genomic zebrafish DNA DNA using KAPA Long Range HotStart PCR kit (Kapa Biosystems) and cloned into the E1b:GFP:AC-DS vector (102417 Addgene (Chong-Morrison et al. ...
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bioRxiv - Microbiology 2020Quote: ... gRNA encoding DNA sequences were amplified in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems) and sequenced on an Illumina NextSeq (High Output ...
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bioRxiv - Cell Biology 2020Quote: ... qRT-PCR was performed with Kapa SYBR Fast qPCR Kit Master Mix (2×) Universal (Kapa Biosystems Ltd., Wilmington, MA, USA) on a CFX connect real-time system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Microbiology 2022Quote: ... and by quantitative PCR (qPCR) using a KAPA Library Quantification Kit for Illumina sequencing platforms (Kapa Biosystems, Roche, Wilmington, MA). Samples were multiplexed and sequenced with a 15% spike-in of PhiX DNA in an Illumina NovaSeq platform (Illumina ...
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bioRxiv - Cancer Biology 2022Quote: ... genomic (g)DNA was extracted both from primary Irf4−/− tumours as well as FACS-sorted control B220+mIgM- BM fr.A-D cells using the High Pure PCR Template Preparation kit from Roche (11796828001). Integrity of resultant gDNA was confirmed in a 2 % Agarose gel ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, no. KK4610). Relative expression levels were normalized to human ACTB or mouse Actb ...
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bioRxiv - Microbiology 2020Quote: ... The membrane was hybridized with digoxigenin-labeled DNA probes synthesized with a PCR DIG probe synthesis kit (Roche Molecular Biochemicals) as recommended by the supplier ...
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bioRxiv - Bioengineering 2021Quote: Biotin-labeled dsDNA template was first amplified from the plasmid encoding the template design with biotin-labeled primers using KAPA HiFi HotStart ReadyMix PCR Kit (Roche) for 35 cycles (98°C for 20 s ...
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bioRxiv - Cancer Biology 2022Quote: ... 500-1000 ng of sheared DNA was used as a template for a 6-cycle PCR to construct a fragmented library using the KAPA HTP Library Preparation Kit (Roche). Exome enrichment was performed using SeqCap EZ Enrichment Kit (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... according to manufacturer’s protocol. Quantitative PCR was performed using SensiMixTM II Probe Kit (Cat#. BIO-83005) with Universal ProbeLibrary (Roche Diagnostics), and the CFX96 Touch System (Bio-Rad) ...
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bioRxiv - Developmental Biology 2022Quote: ... The PCR products were then purified and reverse transcribed with digoxigenin labeling using a DIG RNA labelling kit (Roche, Switzerland). For dissected adult pituitary or 5-8 dpf larvae ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Libraries were subsequently index-amplified for 20 cycles using a KAPA HiFi HotStart PCR kit (Kapa Biosystems, Wilmington, Ma. USA) in 50-μl reactions following the manufacturer’s guidelines ...
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bioRxiv - Genetics 2019Quote: ... located in exon 18 and reverse primer 5’-TTGGTGGCTACAAAGACGTG-3’ located in exon 22 using the One Tube reverse transcription-PCR reaction kit (Roche) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2019Quote: The relative concentration of the resulting DNA ligated-probes was assessed by quantitative PCR (qPCR) using the LightCycler 480 SYBR Green I Master kit (Roche). 3.5 µl of diluted samples was used to assemble a 10 µl reaction and run on a Light Cycler 480 II (Roche ...
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bioRxiv - Plant Biology 2021Quote: ... A PCR master mix was prepared with the LightCycler 480 SYBR Green I Master Kit (Roche Applied Science, Mannheim, Germany). Each reaction was performed in a total volume of 10 μL ...
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bioRxiv - Cancer Biology 2020Quote: ... Exon 1 and exon 3 digestion products were purified using the High pure PCR product purification kit (Roche, Basel, Switzerland) according to the manufacturer’s protocol ...