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Citations for Roche :
2051 - 2100 of 7604 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: ... Cells were then harvested and genomic DNA was extracted with a High Pure PCR Template Preparation Kit (Roche 11796828001). MinION runs were performed on R9 with 400-500ng purified DNA per sample following library preparation with Ligation Sequencing Kit 1D (ONT SQK-LSK108) ...
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bioRxiv - Genomics 2020Quote: ... Bisulfite converted DNA was then used as template for PCR amplification with KAPA HiFi HotStart Uracil kit (Roche, KK2801) using primers surrounding Site 1 in intron 8 of the Fto gene ...
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bioRxiv - Genomics 2019Quote: All 5’ fragments were amplified off the array using HSSF-ATGC and DO_15R_PU (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) and stopped before plateauing ...
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bioRxiv - Genomics 2020Quote: ... The fragmented DNA was then ligated with dual-indices using a KAPA Hyper prep PCR-free library kit (Roche) according to the manufacturer’s directions ...
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bioRxiv - Cell Biology 2020Quote: ... The barcoded sequencing libraries were quantified by quantitative PCR using the KAPA Library Quantification Kit (KAPA Biosystems, Wilmington, MA). Sequencing libraries were loaded on a NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2021Quote: ... The PCR products were used to synthesize dsRNA using a T7 RNA Polymerase Kit (Sigma-Aldrich RPOLT7-RO ROCHE). Generated dsRNAs were treated with TURBO DNA-free Kit (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: Genomic DNA was extracted from BHI broth cultures using the High Pure PCR template preparation kit (Roche, Potsdam, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... mRNA expression levels were determined by qRT-PCR with KAPA SYBR FAST qPCR Master Mix Kit (Kapa Biosystems, KK4610). cDNA was synthesized from human cells using SuperPrep II Cell Lysis & RT Kit (TOYOBO ...
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bioRxiv - Neuroscience 2022Quote: ... and the pool was quantified by qRT-PCR using the KAPA Library Quantification Kit - Illumina/Universal (KAPA Biosystems, KK4824) in combination with the Life Technologies Viia 7 real time PCR instrument ...
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bioRxiv - Plant Biology 2022Quote: ... qRT-PCR reactions were performed using the KAPA SYBR FAST qPCR Master Mix (2X) Kit (KAPA Biosystems, Wilmington, USA) on the QuantStudio 3 Real-Time PCR System (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... was introduced by site directed mutagenesis on C-terminal SNAP-tagged H3.1 (HIST1H3C coding sequence cloned in pSNAPm) and H3.3 (H3F3B coding sequence cloned in pSNAPm) encoding plasmids with a PCR master kit (Roche) and the primers indicated in Supplementary table 2 ...
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bioRxiv - Molecular Biology 2023Quote: ... Highly sensitive DNAprobes labelled with digoxigenin-dUTP were generated using the PCR digoxigenin probe synthesis kit (Roche Applied Science) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2023Quote: ... Quantitative PCR was carried out on standards and test samples using the LightCycler 480 Probes Master kit (Roche, 04707494001) with inverted terminal repeat probe and primer sequences indicated in Table S4.
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bioRxiv - Genomics 2023Quote: ... Library construction was performed on the 5 ng of pooled PCR amplicons using the KAPA HyperPrep Kit (Roche 07962363001) and NEXTFLEX Unique Dual Index Barcodes (7.5µM final concentration ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and the molarity of the library was measured using quantitative PCR with the KAPA Library Quantification Kit (Roche KK4824) on a BioRad CFX Connect thermal cycler ...
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bioRxiv - Genomics 2023Quote: DNA extraction for sequencing with Illumina was done using High Pure PCR Template Preparation Kit from Roche (Mannheim, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Bioengineering 2023Quote: ... The plasmid backbones and DNA fragments were amplified using the KAPA HiFi HotStart PCR kit (Kapa Biosystems, Boston, USA) and the primers described in Table S3 ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 20 x 106 cells were used for isolating genomic DNA with High Pure PCR Template Preparation Kit (Roche) as described by the manufacturer ...
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bioRxiv - Developmental Biology 2023Quote: Genotyping of ESCs and mouse tails were performed by PCR using KAPA Mouse Genotyping Kit (Roche, Cat. No. KK7302). Specific protocol followed the reagent instructions ...
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bioRxiv - Microbiology 2024Quote: ... six reverse primers (10 μM) and Robust DNA polymerase from the KAPA2G Robust PCR Kit (Kapa Biosystems, Wilmington, MA). Cycling conditions were ...
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bioRxiv - Plant Biology 2024Quote: We fragmented 700 ng of high-molecular-weight DNA using a Covaris S220 sonicator and a WGS library was generated for both species using the KAPA Hyper Prep kit with a PCR-free protocol according to the manufacturer’s instructions (Roche). We applied final size selection using a 0.7-fold ratio of AMPureXP beads ...
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bioRxiv - Molecular Biology 2024Quote: Analysis of ChIP-purified DNA by quantitative PCR (qPCR) was performed using KAPA SYBR Fast qPCR kit (KAPA Biosystems) and StepOnePlus real-time PCR systems (Life Technology ...
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bioRxiv - Bioengineering 2020Quote: ... PCR amplification was performed using KAPA HiFi PCR Mix (Kapa Biosystems KK2602). Specifically ...
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bioRxiv - Bioengineering 2022Quote: ... PCR was performed using 12.5 μl KAPA2G Fast PCR mastermix (KAPA Biosystems), containing reaction buffer ...
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bioRxiv - Immunology 2020Quote: ... qRT-PCR was performed using the LightCycler 480 II PCR System (Roche) with program as followed ...
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bioRxiv - Neuroscience 2019Quote: ... qRT-PCR was performed using the SYBR Green PCR Master Mix (Roche) on a ViiA7 Real-Time PCR system (Applied Biosystems) ...
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bioRxiv - Plant Biology 2019Quote: ... Quantitative PCR (qRT– PCR) was performed on a 480 LightCycler thermocycler (Roche) using the manufacturer’s instructions with Light cycler 480sybr green I master (Roche ...
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bioRxiv - Microbiology 2019Quote: ... A second PCR step using the Expand High Fidelity PCR System (Roche) allowed the incorporation of the Illumina MiSeq version 2 indexes and adapters ...
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bioRxiv - Immunology 2021Quote: ... qRT-PCR was performed using the LightCycler 480 II PCR System (Roche) with the following program ...
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bioRxiv - Immunology 2019Quote: ... 2.5 ×105 haemocytes from each treatment/time point were added to lysis buffer (50mM potassium phosphate, 2mM EDTA, 1mM DTT and a proteinase inhibitor cocktail (Roche cOMPLETE™ Mini kit) and centrifuged at 14,000 × g for 10 minutes at 4°C ...
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Nucleo-cytoplasmic shuttling of splicing factor SRSF1 is required for development and cilia functionbioRxiv - Molecular Biology 2020Quote: ... This was followed by SybrGreen detection system (Lightcycler 2x SybrGreen Mix, Roche, #04707516001).
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bioRxiv - Evolutionary Biology 2021Quote: ... Chromosome slides were subjected to the detection with avidin-FITC (Roche, Basel, Switzerland) and then counterstained with DAPI in VECTASHIELD Antifade Mounting Medium (Vector Laboratories ...
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bioRxiv - Biochemistry 2022Quote: ... QPCR was performed using Roche’s SYBER Green detection in the Lightcycler 480 (Roche). Quantification of mRNA was performed by relative normalization to the constitutive gene GAPDH ...
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bioRxiv - Plant Biology 2021Quote: ... Detection was performed with the BM-chemiluminescence blotting substrate (Roche catalogue no 11500708001) and FusionFx7 imaging system (PeqLab) ...
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Integrative analyses of the RNA modification machinery reveal tissue- and cancer-specific signaturesbioRxiv - Molecular Biology 2019Quote: ... Detection of the labelling was performed using the ChromoMAP DAB (760-159, Roche). Sections were counterstained with hematoxylin (760-2021 ...
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bioRxiv - Microbiology 2020Quote: ... the labeled DNAs were detected using a CSPD-based chemiluminescence detection system (Roche) (26) ...
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bioRxiv - Cancer Biology 2022Quote: ... Detection of tagged constructs was done using: HA-peroxidase antibody (Roche, ref: 12013819001), anti- TAP antibody (Thermofisher Scientific ...
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Glutamine synthetase mRNA releases sRNA from its 3’UTR to regulate carbon/nitrogen metabolic balancebioRxiv - Microbiology 2022Quote: ... The RNAs were visualized by using a detection system with digoxigenin (DIG) (Roche) and then captured using the imaging system ChemiDoc XRS Plus (BioRad) ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were lysed in Cell Death Detection Lysis Buffer (Roche, Basel, Switzerland) for analysis of insulin content ...
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bioRxiv - Developmental Biology 2023Quote: ... Detection was performed using an anti-digoxigenin AP-conjugate antibody (Roche, Cat# 11093274910) followed by an NBT/BCIP reaction (Roche) ...
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bioRxiv - Plant Biology 2024Quote: ... Chemiluminescent detection of DIG-labelled DNA was carried using CDP-Star solution (Roche) before detection.
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR® FAST Master Mix (KAPA Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... RT-qPCRs were performed using 1× LightCycler 480 SYBR Green I Master (Roche), 0.5 μM primers (Suppl ...
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bioRxiv - Cancer Biology 2020Quote: ... RT-qPCR was performed on a LightCycler® 480 II (Roche Life Science) using SYBR® Premix Ex Taq(tm ...
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bioRxiv - Molecular Biology 2022Quote: ... RT-qPCR was subsequently performed as described [23] using Sybr green (Roche, 06924204001) on The LightCycler 480 System ...
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bioRxiv - Genomics 2022Quote: ... RT-qPCR was performed using LightCycler 480 SYBR Green I master mix (Roche). For quantification of gene expression ...
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bioRxiv - Immunology 2022Quote: ... slides were blocked at RT for 1 h in 1x Blocking reagent (Roche) and incubated at 4°C over night with a phospho-histone H2AX (pSer139 ...
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bioRxiv - Plant Biology 2022Quote: ... RT-qPCR assays were carried out on LightCycler LC480 II (Roche Diagnostics, Germany) using SYBR Green Jumpstart Taq Ready mix (Sigma-Aldrich ...
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bioRxiv - Plant Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR master mix (Kapa Biosystems) with gene-specific primers (Supplementary Table 6 ...
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bioRxiv - Immunology 2021Quote: ... RT-qPCR was performed in a LightCycler® 2.0 instrument (Roche, Mannheim, Germany), using 45 cycles of the following program ...