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Citations for Roche :
2301 - 2350 of 8008 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... The RT-qPCR assays were performed using a LightCycler 96 instrument (Roche, Basel, Switzerland) according to the manufacture’s protocol with the THUNDERBIRD SYBR qPCR Mix (TOYOBO Co ...
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bioRxiv - Cancer Biology 2022Quote: ... and RT-qPCR was performed using the Universal Probe Library System (Roche, Penzberg, Germany). cDNA ...
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bioRxiv - Plant Biology 2022Quote: ... to perform RT-qPCR in a LightCycler 480 II instrument (Roche Applied Science, UK). The cycle conditions were ...
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bioRxiv - Immunology 2020Quote: ... GAPDH was analyzed by one-step SYBR Green RT-qPCR (Kapa Biosystems, Wilmington, MA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... One-step RT-qPCR was perfomed with LightCycler 480 RNA Master Hydrolysis Probes (Roche) and commercially available CD55 and ACTB TaqMan primers and probes during 5 min at 60°C ...
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bioRxiv - Developmental Biology 2021Quote: ... RT-qPCR was performed using KAPA SYBR FAST qPCR master mix reagents (Kapa Biosystems). Relative mRNA expression was normalized using rpl8 to calculate ΔCTs (threshold cycles) ...
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bioRxiv - Microbiology 2022Quote: ... RT-qPCR was performed with the LightCycler 480 SYBR Green I Master (Roche, 04707516001) in a LightCycler 480 II (Roche ...
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bioRxiv - Microbiology 2024Quote: ... The RT-qPCR was carried out using SYBR green master mix (Roche Applied Science) in a QuantiFast light cycler (Applied Biosystems) ...
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bioRxiv - Genetics 2023Quote: ... The RT-qPCR assay was carried out using FastStart SYBR Green Master Mix (ROCHE) as described previously (Alsayegh et al. ...
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bioRxiv - Plant Biology 2023Quote: ... Expression analysis was performed using RT-qPCR in a Roche LC480 machine (Roche, USA) using the preprogrammed “SYBR green-I 96 well program” ...
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bioRxiv - Microbiology 2023Quote: ... RT-qPCR reaction and results analysis were performed on a LightCycler 96 instrument (Roche).
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bioRxiv - Microbiology 2023Quote: ... followed by a RVFV-specific RT-qPCR amplification [32] in a Lightcycler L96 (Roche) equipment ...
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bioRxiv - Cancer Biology 2024Quote: ... RT-qPCR analysis was performed with LightCycler® 480 SYBR Green I Master (Roche) and primers designed using the Primer-BLAST tool [97] and purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... For each preamplification PCR reaction we used the FastStart High Fidelity PCR System (Roche) at the following concentrations ...
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bioRxiv - Genomics 2019Quote: ... Quantitative PCR analysis was performed using SYBR Green Quantitative PCR Master Mix (Roche 0692404001) in a Light Cycler 96 Real Time PCR system (Roche) ...
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bioRxiv - Neuroscience 2021Quote: ... Q-PCR was performed on a LighCycler Q-PCR machine (Roche, Burgess Hill, UK) following published methods (Read et al. ...
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bioRxiv - Neuroscience 2021Quote: ... qRT-PCR was performed on a LighCycler Q-PCR machine (Roche, Burgess Hill, UK) following published methods (Read et al. ...
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bioRxiv - Genetics 2020Quote: ... The inverse PCRs were performed with the expand long template PCR system (11681842001, Roche) using 30 amplification cycles (94°C 2 min ...
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bioRxiv - Developmental Biology 2021Quote: ... eight independent PCRs were performed using the Expand Long Template PCR System (Roche, 11759060001) that were subsequently pooled and purified using AMPure XP beads (Beckman Coulter ...
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bioRxiv - Genomics 2022Quote: ... PCR was performed by adding 10μl 2×HiFi PCR mix (Kapa Biosystems, Cat. 7958927001) and 0.5μl 60mM SINGV6 primer and running the following program ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Quantitative PCR (qRT-PCR) was performed using Light Cycler 480 instrument (Roche Life Sciences). Anti-Flag® M2 affinity agarose gel was from Sigma-Aldrich (A2220) ...
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bioRxiv - Cell Biology 2023Quote: ... we used PCR with a proofreading Taq polymerase (Roche, Expand Long Template PCR System) to amplify the C-terminal region (∼0.5-1 kb ...
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bioRxiv - Pathology 2023Quote: Quantitative PCR (qPCR) was performed using the Quantitect SYBR green PCR Master mix (Roche) with 10 ng of DNA and 0.5 μM primers in a final volume of 10 μl ...
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bioRxiv - Plant Biology 2019Quote: ... Adapter-ligated cDNA fragments were enriched and amplified with 15 cycles of PCR using the HiFi NGS Library Amplification kit from KAPA Biosystems. Libraries with were multiplexed at equimolar concentration and sequenced on the Illumina HiSeq 2500 for 1×40 bases ...
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bioRxiv - Cancer Biology 2021Quote: ... Indexed libraries that met appropriate cut-offs for both quantity and quality were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Neuroscience 2020Quote: ... The molarity of adapter-modified molecules was defined by quantitative PCR using the Kapa Biosystems Kapa Library Quant Kit (Roche-KK4824). Individual libraries were normalized to 1.30 nM in preparation for Illumina sequence analysis ...
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bioRxiv - Developmental Biology 2021Quote: ... and the whole converted DNA was used as input for library preparation using the Accel-NGS Methyl-Seq DNA Library Kit (Swift Biosciences) according to the recommended protocol and indexing PCR using 2X KAPA HiFi HotStart Uracil+ ReadyMix (KAPA Biosystems). Indexed ...
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bioRxiv - Cell Biology 2022Quote: Genomic DNA from OS/PDB tissues and matched healthy bone marrow samples was isolated using the High Pure PCR Template Preparation Kit following the manufacturer’s instructions (Roche Life Science). Genomic DNA from cell lines (MC3T3 and RPE1 ...
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bioRxiv - Genomics 2019Quote: ... was the substrate for a 10 μL qPCR reaction using either Taq DNA polymerase or KAPA HiFi (KAPA HiFi HotStart PCR kit, Kapa Biosystems) according to the manufacturer’s protocol with barcoded P5 and P7 sequencing primers at 0.5 mM and with 1x EvaGreen dye (Biotium ...
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bioRxiv - Developmental Biology 2019Quote: ... 1 µg of linearized pCR©II-Tc-mef2 or pCR©II-Tc-duf/kirre was in vitro transcribed utilizing the DIG RNA labelling kit (Roche) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Quality control for primer target amplification and performance in multiplex PCR was assessed by screening each primer pair in singleplex reactions using gDNA from PC3 or DU145 cells (10 ng) as template (see below) using the HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems). Reactions were supplemented with uracil N-glycosylase (0.2 U ...
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bioRxiv - Molecular Biology 2020Quote: ... A-tailed and ligated to an adaptor in the form of a uracil-containing stem-loop using the KAPA HTP Library Preparation Kit PCR Free (KAPA Biosystems). Lambda exonuclease (New England Biolabs ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative reverse transcription PCR was performed using Eppendorf Realplex2 Mastercycler with reaction mixtures prepared with KAPA SYBR Fast Universal qPCR Kit (KAPA Biosystems). The PCR primers for KIF1A ...
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bioRxiv - Genomics 2021Quote: ... target sites were amplified directly from genomic DNA in the lysate using a KAPA HiFi HotStart PCR kit (KAPA Biosystems, KK2501) as previously described18 ...
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bioRxiv - Plant Biology 2021Quote: ... a digoxigenin-labeled probe corresponding to 415 bp of the ACMV AC1 gene was generated using a PCR DIG Probe Synthesis kit (Roche Diagnostics) and the primer pair ...
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bioRxiv - Microbiology 2020Quote: ... The sgRNA expression cassettes were amplified from gDNA in a two-step nested PCR using KAPA HiFi HotStart ReadyMixPCR Kit (Kapa Biosystems). For PCR1 ...
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bioRxiv - Plant Biology 2021Quote: ... The obtained library was amplified by emulsion polymerase chain reaction (PCR) using a GS FLK Titanium SV/LV emPCR Kit (Lib-L; Roche Diagnostics), added to a GS FLK Titanium PicoTiterPlate (Roche Diagnostics) ...
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bioRxiv - Zoology 2021Quote: ... a fragment of the EHP small subunit ribosomal RNA (SSU rRNA) gene was used to generate an EHP probe with a PCR-DIG labeling kit (Roche, Germany) ENF779 and ENR779 (Tangprasittipap et al. ...
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bioRxiv - Pathology 2021Quote: ... The obtained individual libraries were also quantified by an Agilent 2100 Bioanalyzer using a DNA7500 LabChip kit and an equimolecular pool of libraries were titrated by quantitative PCR using the “Kapa-SYBR FAST qPCR kit for LightCycler 480” (Kapa BioSystems) and a reference standard for quantification ...
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bioRxiv - Molecular Biology 2020Quote: ... Poly-A enriched libraries were generated using the Illumina TruSeq Standard mRNA preparation kit and checked via the Bioanalyzer and quantitative PCR (KAPA Biosystems). Paired-end reads (100 nt ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Indexed libraries (that met appropriate cut-offs for both) were quantified by qRT-PCR using a commercially-available kit (KAPA Biosystems), and insert size distribution was determined using a LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Microbiology 2020Quote: ... the PCR products digested with indicated restriction enzymes and ligated into the pBAD28 vector using the Rapid DNA Ligation Kit (Roche Diagnostics). Inserted DNA sequences were confirmed by DNA sequencing (StarSeq).
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bioRxiv - Microbiology 2019Quote: ... and Southern hybridization with blaKPC and rep IncFIIK DIG-labelled probes (16) prepared using published primers (35,36) and the PCR DIG Probe Synthesis Kit (Roche, Mannheim, Germany) following manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: All 192 bp enhancers were amplified from the array using HSSF-ATGC and HSS-R-clon (Supplemental Table 8) with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) with SYBR Green (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2019Quote: PCR amplification of RNA editing containing sequence were performed by using cDNA and gDNA as templates with KAPA HiFi HotStart ReadyMix PCR kit (Roche, Germany) and the following program ...
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bioRxiv - Microbiology 2021Quote: ... were used for the purification steps and the libraries were amplified (9 amplification cycles) with the KAPA HiFi PCR Kit (Kapa Biosystems) in KAPA HiFi Fidelity Buffer (5x) ...
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bioRxiv - Genetics 2020Quote: Genomic DNA was isolated from well-developed leaves of 14-day plants according to the modified CTAB procedure (Murray and Thompson, 1980) or by using the KAPA3G Plant PCR Kit (Kapa Biosystems). The PCR for genomic DNA isolated by CTAB was carried out in a 25 ml reaction mixture using Platinum Taq DNA Polymerase (Invitrogen ...
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bioRxiv - Genomics 2019Quote: All libraries were amplified off the array using the primers indicated in Supplemental Table 10 with KAPA HiFi HotStart Uracil+ ReadyMix PCR Kit (Kapa Biosystems) as described above ...
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bioRxiv - Genomics 2021Quote: ... Strand-specific Illumina cDNA libraries were prepared using the KAPA Stranded RNA-Seq library preparation kit with 10 cycles of PCR (KAPA Biosystems). Library quality was assessed by BioAnalyzer (Agilent ...
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bioRxiv - Molecular Biology 2019Quote: ... and an equimolecular pool of libraries was prepared and titrated by qRT-PCR using the “Kapa-SYBR FAST qPCR kit forLightCycler480” (Kapa BioSystems, Fritz Hoffmann-La Roche ...