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Citations for Roche :
2251 - 2300 of 8008 citations for rno mir 143 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: Pitx1 WISH were performed on 40-45 somite stage mouse embryos (E12.5) using a digoxigenin-labeled Pitx1 antisense riboprobe transcribed from a cloned Pitx1 probe (PCR DIG Probe Synthesis Kit, Roche), as previously described in (Kragesteen et al ...
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bioRxiv - Cell Biology 2019Quote: All genotyping was done using the HotStart Mouse Genotyping Kit with its instructed PCR reaction set up from KAPA Biosystems. For the NF1Afl/fl genotype ...
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bioRxiv - Genetics 2021Quote: We extracted DNA from nail samples using the ISOHAIR (Nippon Gene) and oral mucosa samples using the High Pure PCR Template Preparation Kit (Roche).
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bioRxiv - Neuroscience 2021Quote: ... the circular DNA fragment containing the shotV104 breakpoint region was amplified using a High Fidelity PCR Kit (Eppendorf and Roche). PCR products were gel-extracted ...
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bioRxiv - Genomics 2019Quote: ... Genome libraries were prepared using the KAPA Hyper Library Preparation Kit with Standard PCR Library Amplification (Kapa Biosystems, Wilmington, MA) and sequenced on an Illumina MiSeq using V3 sequencing chemistry (Illumina Inc. ...
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bioRxiv - Microbiology 2021Quote: ... Radiolabeled probe was generated by purifying a digested (Ncol; 1 cut) pCpG vector DNA using High Pure PCR product Purification Kit (Roche) followed by radiolabeling the probe using Random Prime DNA labeling kit (Roche) ...
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bioRxiv - Immunology 2022Quote: ... and 11-week infected half brains and liver lobes of mice (n=4/group) was extracted and purified using a High Pure PCR Template Prep Kit (Roche). DNA concentration of each sample was determined via NanoDrop ...
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bioRxiv - Cell Biology 2022Quote: Following library construction as per manufacturer’s instructions ST libraries were quantified using the KAPA-Illumina PCR quantification kit (KAPA Biosystems) and pooled at 4nM concentration with a sample ratio corresponding to the surface area of tissue coverage obtained from the H&E imaging ...
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bioRxiv - Cancer Biology 2022Quote: The activity of telomerase in the cell lines was detected using the TeloTAGGG telomerase PCR ELISA Kit (Roche, Cat# 12013789001). The assay was performed according to the manufacturer’s instructions and repeated in triplicate ...
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bioRxiv - Genomics 2022Quote: ... and P5 and P7 adapters were ligated per pool following the KAPA HiFi Hotstart PCR kit standard protocol (Kapa Biosystems). Pooled samples were then combined into one sample and cleaned as before ...
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bioRxiv - Microbiology 2022Quote: ... the ligated product was amplified by 12 PCR cycles using the Kapa Hifi Hotstart NGS library amplification kit (Kapa Biosystems), followed by purification with 0.6×AMPure XP ...
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bioRxiv - Plant Biology 2022Quote: ... and 72°C for 20 s) containing the Spartina cDNAs with the PCR DIG Probe Synthesis Kit (Roche, Cat.11636090910) using the SaHKT1 transcripts specific primers (Forward primer ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic PCR for ATP13A2 floxed or KO alleles was performed using 100 ng genomic DNA and the Kapa2g Fast HotStart PCR Kit (Roche). PCR primers included a forward primer (5’-CTGCAGCTTCGAGAGGAAAG-3’) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The short insert paired-end libraries for the whole genome sequencing were prepared with PCR free protocol using KAPA HyperPrep kit (Roche). The library was sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Evolutionary Biology 2023Quote: Antisense probes were synthesized using purified PCR products (1 μg per reaction) (supplementary table S16) and DIG RNA labeling Kit (T7) (Roche), following the manufacturer instructions ...
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bioRxiv - Systems Biology 2023Quote: ... the entire eluate was used as input for a 300 μl PCR reaction mastermix with the KAPA HiFi HotStart DNA Polymerase kit (Roche Sequencing Solutions Inc ...
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bioRxiv - Microbiology 2023Quote: ... Probes were obtained by PCR using the primers pGK-S55 and pGK-S53 (Table S1) and labelled with the PCR DIG Probe Synthesis Kit (Roche) according to the manufacturer and revealed using the DIG Luminescent Detection Kit and DIG Easy Hyb (Roche).
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA was extracted from mouse tail tip or ear notch biopsies using the High Pure PCR Template Preparation Kit (Roche) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... The equally mixed pool of VH and Vκ/Vλ gene fragments was subjected to overlap extension PCR to generate scFv genes using a KAPA Biosystems kit (Roche) with overlap extension primers (No ...
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bioRxiv - Molecular Biology 2024Quote: ... Indexed libraries that met appropriate cutoffs for both were quantified by qRT-PCR using a commercially available kit (KAPA Biosystems) and insert size distribution was determined with the LabChip GX or Agilent Bioanalyzer ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets from the three different cell types were assayed for telomerase activity according to the TeloTAGGG Telomerase PCR ELISA Kit (11854666910; Roche).
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bioRxiv - Genetics 2020Quote: ... PCR amplification was carried out using the Expand Long Template PCR System (Roche) with the following thermocycler conditions ...
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bioRxiv - Immunology 2019Quote: ... PCR reactions were carried out using FastStart™ High Fidelity PCR System (Roche) with the following cycling conditions ...
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bioRxiv - Immunology 2019Quote: ... PCR reactions were carried out using FastStart™ High Fidelity PCR System (Roche) with the following cycling conditions ...
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bioRxiv - Cancer Biology 2019Quote: ... PCR reactions were performed using the Expand Long Template PCR system (11759060001, Roche) with the following PCR conditions ...
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bioRxiv - Microbiology 2019Quote: ... The PCR reaction mixture (Expand High Fidelity PCR system, Roche Diagnostics, Mannheim, Germany) was composed of 10 μ1 of cDNA ...
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bioRxiv - Developmental Biology 2019Quote: ... Quantitative PCR was conducted using the SYBR Green PCR Master Mix (Roche, 04673514001) on a StepOne Plus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Microbiology 2019Quote: ... Amplicon PCR reactions were carried out using the Faststart PCR system (Roche, Switzerland). The 25-μl PCR mix was composed of 3 ng/μl DNA ...
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bioRxiv - Neuroscience 2020Quote: ... PCR was performed by adding 10 μL 2X HiFi PCR Mix (Kapa Biosystems) and 0.5 μL 60 μM SINGV6 primer and incubating at 98°C for 3 m ...
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bioRxiv - Neuroscience 2021Quote: ... PCR was performed by adding 10 mL 2X HiFi PCR mix (Kapa Biosystems) and 0.5 ml 60 mM SINGV6 primer with the following conditions ...
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bioRxiv - Developmental Biology 2020Quote: ... PCR amplification was performed using Expand High Fidelity PCR System (Roche. Indianapolis, IN). All primers were purchased from Integrated DNA Technologies (Coralville ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification was then performed using FastStrat High Fidelity PCR System (Roche, SUI). The light and heavy chain genes were amplified from the cDNA by PCR using the primer pairs from VK ...
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bioRxiv - Immunology 2020Quote: ... Quantitative PCR was performed using the QuantiTect SYBR green PCR Master mix (Roche) with 10 ng of DNA and 0.5 μM primers in a final volume of 10 μl ...
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bioRxiv - Immunology 2021Quote: ... PCR amplification were carried out using KAPA HiFi PCR Mastermix (Kapa Biosystems KK2602) with 2.00 beads per 50 μL reaction volume ...
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bioRxiv - Neuroscience 2022Quote: ... PCR was performed by adding 10 μL 2X HiFi PCR mix (Kapa Biosystems) and 0.5 μl 60 μM SINGV6 primer with the following conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR was performed using SYBR Green PCR Master Mix (KK4602, Kapa Biosystems) and a StepOnePlus Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2021Quote: ... The signal was revealed using the alkaline phosphatase detection reagents NBT/BCIP (Roche, 11383221001).
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bioRxiv - Cell Biology 2021Quote: ... Detection occurred via an anti-digoxygenin antibody coupled to alkaline phosphatase (1:10,000) (Roche).
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bioRxiv - Neuroscience 2020Quote: ... and detection of digoxigenin-labeled probes were carried out according to manufacturer’s protocol (Roche), using anti-digoxigenin antibody conjugated to alkaline phosphatase for detection ...
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bioRxiv - Immunology 2022Quote: Lactate dehydrogenase (LDH) release from cells was measured using the Cytotoxicity Detection KitPLUS (Roche), according to the manufacturer’s protocol ...
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bioRxiv - Genetics 2020Quote: ... DIG Labelling and chemiluminescent detection were performed as per the manufacturer’s instructions (Roche, Germany).
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bioRxiv - Neuroscience 2021Quote: ... Immunological detection was carried out with anti-DIG antibody conjugated with alkaline phosphatase (Roche). Color reaction was conducted with NBT/BCIP ...
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bioRxiv - Cancer Biology 2021Quote: ... Histone-associated DNA-fragment levels were analyzed using the Cell Death Detection ELISAPLUS (Roche; Cat ...
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bioRxiv - Microbiology 2024Quote: ... Detection of mRNA was performed with anti-DIG-alkaline-phosphatase-conjugated antibody (Roche Diagnostics) and the alkaline phosphatase substrate 5-bromo-4-chloro-3-indolyl phosphate (BCIP ...
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bioRxiv - Developmental Biology 2023Quote: ... Probe detection was by tissue incubation in 1:3000 anti-DIG alkaline phosphatase (Roche) in block buffer for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... as secondary antibodies and detection with the Lumi-Light Plus Western Blotting Substrate (Roche). Images were acquired and quantitated with an ImageQuant LAS 4000 chemiluminescence imager (GE Healthcare).
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bioRxiv - Evolutionary Biology 2021Quote: ... The RT-qPCR assays were performed using a LightCycler 96 instrument (Roche, Basel, Switzerland) according to the manufacture’s protocol with the THUNDERBIRD SYBR qPCR Mix (TOYOBO Co ...
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bioRxiv - Plant Biology 2019Quote: ... 5 μl of KAPA SYBR Fast RT-qPCR solution (Kapa Biosystems, Inc., Woburn, MA), 0.5 μl of 10 μM forward and reverse primers ...
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bioRxiv - Genomics 2021Quote: ... RT-QPCR assays were performed on the Roche LightCycler 480 system (Roche Diagnostics, France). The assays were carried out in a reaction mix of 10 μL containing 2.5 μL of cDNA (diluted 5 times) ...
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bioRxiv - Cell Biology 2021Quote: ... RT-qPCR was performed with Sybr Green® Lightcycler Green I Master mix (Roche), using a Lightcycler 480 instrument and associated software ...