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Citations for Roche :
2201 - 2250 of 2327 citations for Recombinant Human Epithelial Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Primary human macrophages were washed once with ice cold PBS prior to harvest of cells with TBS supplemented with protease inhibitors (Complete®, Roche) and phosphatase inhibitors (PhosStop® ...
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Quantitative Comparison of Presenilin Protein Expression Reveals Greater Activity of PS2-γ-SecretasebioRxiv - Cell Biology 2023Quote: ... Cells were scrapped into 100 µl of RIPA lysis buffer (Astral Scientific 786-490) supplemented with protease inhibitor cocktail (Roche 11697498001) and transferred to microfuge tubes ...
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bioRxiv - Cancer Biology 2023Quote: The cellular metabolic viability was measured by NADH-dependent mitochondrial dehydrogenase activity using Cell Proliferation Kit II (XTT) (Roche, Basel, Switzerland) according to the manufacturer’s protocol by measuring the absorbance at 492nm in BioTek Synergy HTX multimode reader ...
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bioRxiv - Cancer Biology 2023Quote: ... This plasmid was digested with PacI and transfected into HEK293 cells using X-tremeGENETM HP DNA transfection reagent (Roche, Basel, Switzerland) to produce infectious viral particles ...
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bioRxiv - Cell Biology 2023Quote: ... samples were covered with the staining mixture at 37 °C for 1 h following instructions of the In Situ Cell Death Detection Kit (Roche, 11684795910). After the TUNEL reaction ...
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bioRxiv - Developmental Biology 2023Quote: TUNEL staining was performed according to the manufacturer’s protocol with minor modifications (In Situ Cell Death Detection Kit, TMR red; catalogue number 12156792910; Roche, Mannheim, Germany). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... The cell pellet was resuspended in chilled lysis buffer (Bugbuster) containing one dissolved cOmplete™ EDTA-free Protease Inhibitor cocktail tablet (Roche). The cell lysates were clarified by centrifugation (11,000 × g ...
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bioRxiv - Cell Biology 2023Quote: ... HL-60s were differentiated into a neutrophil-like state by taking an aliquot of cells in their culturing medium and supplementing with an equal volume of Nutridoma-CS (Roche #11363743001) and DMSO diluted in RPMI such that that the final concentrations were 0.2 million/mL HL-60 cells ...
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bioRxiv - Pathology 2023Quote: Cells or tissues were homogenized in RIPA buffer supplemented with protease inhibitors and phosphatase inhibitor cocktails (Roche Diagnostics, Indianapolis, IN, USA). Western blot analysis was performed according to the routine procedure ...
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bioRxiv - Neuroscience 2023Quote: ... 300,000 cells were washed twice (20 mM HEPES pH 7.5, 150 mM NaCl, 0.5 mM spermidine, 1× Roche cOmplete protease inhibitors) and attached to 10 ConA-coated magnetic beads (Bangs Laboratories ...
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bioRxiv - Immunology 2023Quote: ... Wells were pre-coated with 100 μL per well of capture anti-histone antibody contained in the Cell Death Detection ELISA kit (1:40 in 1x coating buffer; Roche 11544675001) or anti-myeloperoxidase (MPO ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed 2X with cold DPBS++ and RIPA buffer (Pierce #89900) containing complete EDTA-free protease inhibitor cocktail (Roche #11873580001) was added to wells ...
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bioRxiv - Cell Biology 2023Quote: ... Infected red blood cells were lysed 10 min on ice with 0.1% saponin in PBS complemented with 1x Protease Inhibitors Cocktail (Roche; PBS+PI). Parasites were pelleted and washed with PBS+PI to remove haemoglobin ...
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bioRxiv - Cell Biology 2023Quote: The tissues from treated mice and HFs were used for TUNEL apoptotic detection (In Situ Cell Death Detection Kit, TMR Red, Roche 12156792910) After fixation and antigen retrieval procedures ...
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bioRxiv - Genomics 2023Quote: ... sgRNA- or cDNA-expressing vectors and lentiviral packaging vectors Delta-VPR and CMV VSV-G were transfected into HEK293T cells using XTremeGene 9 transfection reagent (Roche #636478700). The supernatant containing virus was collected 48 h after transfection and passed through a 0.45-µm filter ...
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bioRxiv - Genomics 2023Quote: hTERT RPE-1 cells at 70% confluence were harvested by trypsinization after 2-hour treatment with colcemid (100 ng/ml, Roche 10295892001) to arrest cells in mitosis ...
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bioRxiv - Immunology 2024Quote: Cells proteins were lysed and isolated from cell pellet by RIPA buffer (Thermo, 89901) with cOmplete mini EDTA free protease inhibitor (Roche, 11836170001) on ice incubation for 30 min and centrifugation at 15,000 rpm for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: Cells were washed with phosphate-buffered saline (PBS) and lysed in whole cell lysis (WCL) buffer supplemented with cOmplete protease inhibitor cocktail (Roche Diagnostics), 100 μM sodium orthovanadate (NaO ...
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bioRxiv - Cancer Biology 2023Quote: ... unconcentrated cell culture supernatants were incubated with a rabbit monoclonal anti-glycodelin antibody (clone 12C9, kindly provided by ROCHE, Penzberg, Germany) for 1 h at room temperature prior to incubation with immune cells ...
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bioRxiv - Molecular Biology 2024Quote: ... treated with λPPase or that purified from Calyculin A-treated cells was subjected to proteolytic digestion with 0.01 ng/µl trypsin (Roche, sequencing grade) for 0 ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were washed three times with TBS-T and were incubated with a TUNEL reaction mixture (In Situ Cell Death Detection Kit, TMR red, Roche 12156792910) for 1 h at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then resuspended in 10 ml of cold PBS and incubated with cOmplete mini EDTA-free protease inhibitor (Roche, 11836170001) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... and a sgRNA preceded by a rrk1 leader and followed by a Hammerhead Ribozyme as described in [17] was digested and prepared for gap repair as follows: the vector pJB166 was purified from DH5α bacterial cells using the Genopure plasmid midi kit (Roche, 03143414001) and diluted to 1 μg/μL ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Bioengineering 2024Quote: ... the cells were harvested from the matrices by digesting the collagen with liberase DL research grade (24 U; Roche, Basel, Switzerland). The cells were then fixed with 4% PFA ...
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bioRxiv - Cell Biology 2024Quote: ... assay was performed following our previous publication(4, 55) and the instruction of In Situ Cell Death Detection Kit TMR red (Roche, 12156792910). Cells were cultured on coverslips for 36 h ...
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bioRxiv - Cell Biology 2020Quote: ... HUVEC were pre-treated with siRNAs or drug for 24 hr prior to plating an equal cell number onto the microelectrode surface of the E-plate (E-plate 16, Roche Applied Science). Impedance readings were taken automatically every 5 min for 24 hr.
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bioRxiv - Developmental Biology 2020Quote: ... Fixed embryos were homogenized in 2 ml cell lysis buffer (10 mM Tris-HCl pH 7.5, 10 mM NaCl, 0.3% NP-40, 1x Roche Complete protease inhibitors cocktail) with a Dounce Homogenizer on ice and centrifuged 5 min 2,300g at 4°C ...
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bioRxiv - Pathology 2019Quote: ... and plasma HIV-1 RNA levels were determined in HIV-1-infected persons at the Makerere University-John Hopkins University laboratory using standard cell sorting techniques and the Amplicor HIV-1 monitor test (Roche, version 1.5), respectively ...
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bioRxiv - Cancer Biology 2020Quote: Total RNA was obtained from astrocytoma cell line 1321N1 using TRIzol and reverse-transcribed with Transcriptor Reverse Transcriptase according to the manufacturer’s instructions (Roche Diagnostics, Mannheim, Germany). Full-length human PFKFB3-4 was generated by standard PCR using primers PFKFB3-4 reverse and PFKFB3-4 forward (Table S2) ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were lysed with 4% SDS lysis buffer (4% SDS, 150 mM NaCl, 50 mM triethanolamine pH 7.4, Roche protease inhibitor, benzonase). Protein concentrations were determined by the BCA assay (Pierce) ...
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bioRxiv - Microbiology 2019Quote: ... The PBMCs were fused with cells from the mouse–human fusion partner cell line SPYMEG (Medical & Biological Laboratories, Nagoya, Japan) at a ratio of 10:1 with polyethylene glycol (Roche, Basel, Switzerland). Fused cells were cultured in Dulbecco’s modified Eagle medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: ... G355-5 cells were transfected with 1 to 6 µg DNA in 6 cm dishes using X-treameGENE 9 (Roche, Basel, Switzerland). The 293T/17 cell line was obtained from ATCC (CRL-11268 ...
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bioRxiv - Cell Biology 2021Quote: Two days after transfection, cells were lysed with RIPA Lysis Buffer (P0013C, Beyotime) supplemented with 1x cOmpleteTM EDTA-free Protease Inhibitor Cocktail (Roche, Basel, Switzerland) for 40 min at 4 °C and then clarified by centrifugation at 12,000 × g for 20 min ...
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bioRxiv - Physiology 2022Quote: ... n=3/genotype) were used for terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) to visualize cell apoptosis/death (Roche, Basel, Switzerland). Sections were counterstained with DAPI and visualized using epifluorescence (Nikon Eclipse Ni E800) ...
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bioRxiv - Pathology 2022Quote: We detected DNA fragmentation resulting from apoptotic signaling cascades with the In Situ Cell Death Detection Kit Fluorescein (Roche, 11684795910, Basel, Switzerland) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: Total RNA was extracted from 200μL of VERO cells culture infected by herpes virus (treated or not with hemolymph) using the MagNA Pure extractor (Roche, Basel, Switzerland). The Herpes simplex virus quantitative real time PCR assay was performed was described by Read et al. ...
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bioRxiv - Microbiology 2020Quote: α-GDV1-GFP-DD and α-Pfs16 IFAs were performed with methanol-fixed cells using mouse mAbs α-GFP (1:200) (Roche, #11814460001) and α-Pfs16 (1:500 ...
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bioRxiv - Biochemistry 2021Quote: Cell-free histone levels were assessed in plasma from healthy controls and COVID-19 patients using a photometric enzyme immunoassay (Cell Death Detection ELISAPLUS kit, Roche Applied Science) that measures histone-associated DNA fragments according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... Nucleic acids from these 200μL mosquito pools and from 100μL venous and finger prick whole blood samples in RNAprotect Cell Reagent were isolated using the bead-based MagNAPure LC automatic extractor (Total Nucleic Acid Isolation Kit—High Performance, Roche Applied Science) and eluted in 50μL of water ...
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bioRxiv - Microbiology 2020Quote: ... plasmids were transfected into HeLa cells growing on 35 mm glass bottom dishes using X-treme GENE HP DNA transfection reagent (Roche, Laval, QC) according to manufacturer’s protocols.
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bioRxiv - Immunology 2020Quote: ... Ice cold 1.5mM EDTA was slowly poured into the lysozyme-treated cells and 1x protease inhibitor was added (Roche cat no. 04693159001). The suspensions were sonicated in an ice/water slurry for eight minutes total with the following parameters ...
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bioRxiv - Neuroscience 2020Quote: The extent of cell death following MPP+ treatment with and without LMX1B overexpression was assessed by using the terminal deoxynucleotidyl transferase mediated dUTP-biotin nick end-labeling (TUNEL) method (In situ cell death detection kit, TMR red, Roche, Cat# 12156792910), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: The cell lysates were extracted with RIPA lysis buffer that contained a protease inhibitor cocktail and phosphatase inhibitor cocktail (Roche, Basel, Switzerland). Equivalent concentrations of protein were separated using gel electrophoresis and were then transferred to a PVDF membrane ...
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bioRxiv - Cancer Biology 2022Quote: ... a debris removal step was performed (Miltenyi #130-109-398) and blood cells were removed using a blood lysis buffer (Roche 11814 389001).
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bioRxiv - Microbiology 2019Quote: ... the ORF50 trans-complementing BHK-21 cells were transfected with 2 μg of ΔORF50 BAC DNA using FuGENE HD Transfection Reagent (Roche, Mannheim, Germany). When cells showed a visible CPE ...
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bioRxiv - Microbiology 2019Quote: ... Cell lysates were harvested 20 hours post-infection (hpi) and suspended in RIPA buffer with protease and phosphatase inhibitors (Roche, Thermo Fisher). Samples containing 10-20 µg protein were boiled for 20 min in sample buffer ...
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bioRxiv - Plant Biology 2020Quote: ... In situ nick-end labeling of nuclear DNA fragmentation was performed for 1 h in the dark at 37°C using the In-Situ cell death detection kit (Roche Applied Science) according to the manufacturer’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... Ganglia were mechanically disrupted with a pipette tip until obtaining a single cell suspension in culture media supplemented with DNAse I grade II at final concentration 3mg/ml (Roche, Cat# 104159), which was centrifuged at 1300 rpm (302 rcf ...
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bioRxiv - Molecular Biology 2019Quote: ... the cells were treated with the oligonucleotide mixed with DOTAP liposomal transfection agent (35 μg/ml) (#11811177001, Roche Diagnostics GmbH, Mannheim, Germany) for 3 to 6 hrs (16) ...